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Volume 16, Number 15, Issue of August 1, 1996 pp. 4617-4624
Copyright ©1996 Society for Neuroscience

Selective G-Protein Regulation of Neuronal Calcium Channels

Peter T. Toth1, Lee R. Shekter1, Gloria Hui Ma1, Louis H. Philipson2, and Richard J. Miller1

1 Department of Pharmacological and Physiological Sciences, and 2 Department of Medicine, The University of Chicago, Chicago, Illinois 60637

ABSTRACT
INTRODUCTION
MATERIALS AND METHODS
RESULTS
DISCUSSION
FOOTNOTES
REFERENCES

ABSTRACT

We examined the properties and regulation of Ca channels resulting from the expression of human alpha 1B and alpha 1E subunits stably expressed in HEK293 cells. The ancillary subunits beta 1B and alpha 2/delta were also stably expressed in these cell lines. Ca currents in alpha 1B-expressing cells had the properties of N-type currents. Ca currents in cells expressing alpha 1E exhibited a novel profile that was similar to the properties of the ``R type'' Ca current. Introduction of GTP-gamma -S into alpha 1B cells greatly enhanced the extent of prepulse facilitation of the Ca current, whereas it had only a very small effect in alpha 1E-expressing cells. Activation of somatostatin receptors endogenous to HEK293 cells or kappa  opioid receptors, expressed in the cells after transfection, inhibited Ca currents in alpha 1B-expressing cells. This inhibition was blocked by pertussis toxin and was partially relieved by a depolarizing prepulse. In contrast, no inhibitory effects were noted in cells expressing alpha 1E channels under the same circumstances. HEK293 cells normally contained G-proteins from all of the four major families. Inhibition of Ca currents by kappa  agonists in alpha 1B-expressing cells was enhanced slightly by the cotransfection of several G-protein alpha  subunits. kappa  agonists, however, had no effect in alpha 1E-containing cells, even after overexpression of different G-protein alpha -subunits. In summary, these results demonstrate that there is a large difference in the susceptibility of alpha 1B- and alpha 1E-based Ca channels to regulation by G-proteins. This is so despite the fact that the two types of Ca channels show substantial similarities in their primary sequences.

Key words: Ca channel; G-protein; kappa receptor; patch clamp; HEK293; somatostatin

INTRODUCTION

Regulation by receptors, G-proteins, and second messengers is one of the most widely observed characteristics of Ca channels (Hille, 1994; Dolphin, 1995). For example, the activation of L channels in the heart by cAMP-dependent phosphorylation has been studied extensively, and the physiological significance of these observations is clear (Perez-Reyes et al., 1994). In the nervous system, it has been shown frequently that activation of various G-protein-linked receptors can produce inhibition of N channels (Hille, 1994). Because it is known that N channels play a critical role in the regulation of transmitter release at many synapses (Miller, 1990; Hille, 1994), this process is believed to be a key element in the receptor regulation of synaptic communication, particularly the phenomenon of presynaptic inhibition. Rather than being mediated by a diffusible second messenger, ``rapid'' inhibition of N channels is thought to be attributable to direct effects of G-protein subunits on some part of the Ca channel complex (Hille, 1994). Therefore, this type of N-channel regulation has been described as ``membrane delimited.'' It is not yet clear, however, which subunits of the G-protein heterotrimer actually produce these effects or to which part of the N channel they bind (Sternweis, 1994; Wickman and Clapham, 1995).

N channels, which result from the expression of the alpha 1B Ca channel subunit, are found primarily in neurons and neuroendocrine cells (Williams et al., 1992; Wheeler et al., 1995). This is also the case for at least two other types of Ca channels: the P/Q type, which probably results from the expression of alpha 1A, and the recently described alpha 1E subunit, which may produce a Ca current of the ``R'' type (Williams et al., 1994; Wheeler et al., 1995). Interestingly, the alpha 1A, alpha 1B, and alpha 1E subunits form a Ca channel subfamily and exhibit a high degree of sequence homology (Dolphin, 1995; Wheeler et al., 1995). None of these Ca channels are sensitive to dihydropyridines, which block alpha 1C, alpha 1D, and alpha 1S Ca channels and to which they exhibit less sequence homology (Dolphin, 1995; Wheeler et al., 1995). It is now clear that Ca currents of the P/Q type, which can also support neurotransmitter release (Wheeler et al., 1994), are regulated by G-proteins and receptors in a manner similar to N channels (Mintz and Bean, 1993; Rhim and Miller, 1994; Kanemasa et al., 1995). It is not known at this point, however, whether Ca currents resulting from the expression of alpha 1E are also regulated in this way. Indeed, very little is known about the properties and biological functions of Ca channels resulting from alpha 1E expression. We have now investigated this question and demonstrate that there seem to be considerable differences in the ability of alpha 1B and alpha 1E to be regulated by G-proteins.

MATERIALS AND METHODS

HEK293 cell lines. HEK293 cell lines expressing Ca channels with various subunit compositions were kindly provided by SIBIA Inc. (Williams et al., 1992; Bleakman et al., 1995). Briefly, the cell lines were developed by stable co-transfection of HEK293 cells with human alpha 1, alpha 2Bdelta , and beta  Ca channel subunit expression plasmids. The G1A1 cell line consisted of alpha 1B-1-alpha 2Bdelta -beta 1B subunits. The subunit composition of the A4A2 cells was alpha 1B-1-alpha 2Bdelta -beta 1C. The E52-3 cell line expressed the alpha 1E-type Ca channel with the alpha 1E-3-alpha 2Bdelta -beta 1B subunit composition.

HEK293 cells stably expressing Ca2+-channels were grown in plastic Falcon dishes in DMEM (Life Technologies, Gaithersburg, MD) containing 5% defined bovine serum (HyClone, Logan, UT) plus penicillin G (100 U/ml), streptomycin sulfate (100 µg/ml), and geneticin (500 µg/ml). One day before recording, cells were dissociated by gentle trituration with a fire-polished Pasteur pipette and replated onto poly-L-lysine-coated glass coverslips.

Preparation of G-protein expression plasmids. cDNAs encoding alpha  subunits of Gi1-3 and Go (kindly provided by Randall R. Reed, Johns Hopkins University) were subcloned into the mammalian expression vector pCMV5 (Andersson et al., 1989) and confirmed by DNA sequencing using a modification of the dideoxy-chain termination method (Sequenase 2.0; USB, Cleveland, OH). The cDNA Gsalpha was similarly subcloned into pCMV6b and confirmed by sequencing (Robishaw et al., 1986). The cDNAs encoding G13 and Gq in pcDNA3 were obtained from G. Babnigg (University of Chicago). kappa  opioid receptor cDNA in pCMV5 was a kind gift of Graeme Bell (University of Chicago).

Transfection of HEK293 cells. Monolayers of HEK293 cells not exceeding 75% confluence were dissociated and replated onto poly-L-lysine-coated (Sigma, St. Louis, MO) glass coverslips. Cells were cotransfected with plasmids containing the cDNAs for the kappa  receptor, G-protein, and beta -galactosidase using the standard calcium-phosphate precipitation technique (Ausubel et al., 1993) or transfection kit (Mammalian Transfection Kit; Stratagene, La Jolla, CA).

Analysis of gene and cDNA expression. For patch-clamp experiments, duplicate coverslips were routinely stained for beta -galactosidase expression, with an average transfection efficiency of 40-70% determined by cell counting. Currents were recorded 48-72 hr after transfection.

The expression of the kappa  receptor was detected using the specific antibody (kindly provided by Robert Elde, University of Minnesota) and ABC kit (Vector Laboratories, Burlingame, CA).

The expression of the G-protein subunits was evaluated using PCR-Southern blot and Northern blot analysis, as follows. Total RNA was prepared using the CsCl guanidinium isothiocyanate method (Sambrook et al., 1989). Poly(A+) RNA was prepared using an oligo-dT-Sepharose column or an mRNA purification kit (Pharmacia, Uppsala, Sweden); 15-20 µg of total RNA or 1-2 µg of poly(A+) RNA was loaded per lane. RNA was transferred to Hybond-N+ (Amersham) by overnight blotting. The blots were hybridized using the 32P-labeled-specific oligonucleotide probes (internal sequence) for the individual G-protein alpha  subunits as shown below. In some control experiments (not shown), cDNAs for specific G-proteins were 32P-labeled by random priming and also used as probes. After hybridization [in the solution containing 20% formamide, 5× SSC (1× SSC: 150 mM NaCl, 15 mM sodium citrate), 2.5× Denhardt's solution] for 12-20 hr, the membranes were rinsed in 2× SSC, 0.1% SDS for 10 min at room temperature, washed in 0.5× SSC, 0.1% SDS at 42°C for 20-40 min, and then exposed to Kodak X-omat film for 12 hr.

For PCR-based detection of expression, first-strand cDNA was prepared from total RNA using random primers and Superscript reverse transcriptase (Life Technologies, Grand Island, NY). The following oligonucleotide primer pairs (forward and reverse) were used.

Gialpha 1---Forward: 5'-CTGTGGAAGGACAGCGGTGTG-3'

Reverse: 5'-CAGCAACAGAGAATGTAGTG-3'

Internal: 5'-GCAGTGGGGTAGTAAAAATGCATT-3'

Gialpha 2---Forward: 5'-CCTGTCGGGCGTCATCCGGAG-3'

Reverse: 5'-CCATGCTCCCTGCCTGTTCCC-3'

Internal: 5'-GATGAATCGCATGCATGAGAGC-3'

Gialpha 3---Forward: 5'-GATTAAACGTTTATGGCGAG-3'

Reverse: 5'-GCATGACAGGACCAAGGAATG-3'

Internal: 5'-GAGGATGGCATAGTAAAAGCT-3'

Go---Forward: 5'-CACTGAACCATTCTCTGCAG-3'

Reverse: 5'-TTTGGCCTTTGTAAGACACAC-3'

Internal: 5'-CACTCAGCGGCTATGACCAG-3'

Gs---Forward: 5'-GACCAACCGCCTGCAGGAGGC-3'

Reverse: 5'-GGGCATGATTAACAAAGCAACC-3' (bovine)

Reverse: 5'-GGGCATGATTCACACCGCAACC-3' (rat)

Internal: 5'-CACGCAGTTGATCACCCACC-3'

Gq---Forward: 5'-CTACCCCTGGTTCCAGAACTCC-3'

Reverse: 5'-CACGCTCACAGAGTCCAGGACG-3'

Internal: 5'-GTCGACTAGGTGGGAATACATG-3'

G13---Forward: 5'-CGGGTTTTCAGCAACGTCTCC-3'

Reverse: 5'-TCAGCAGCTGTCAGCCACA-3'

Internal: 5'-CTCTTAAGCAGTGGGGGTCC-3'

The PCR was used under the following conditions: 45 sec at 94°C, 1 min at 55°C, and 1 min at 72°C for 30 cycles, followed by 10 min at 72°C. An aliquot of the PCR sample was analyzed by electrophoresis in 1% agarose. DNAs were transferred to Hybond-N+ (Amersham) and then hybridized with the specific oligonucleotide probe (internal) for each G-protein alpha  subunit shown above, using the conditions described.

Whole-cell patch clamp. The tight-seal whole-cell configuration of the patch-clamp technique (Hamill et al., 1981) was used to record Ca currents. Recordings were made at room temperature (21-24°C). Currents were recorded using an Axopatch 1D (Axon Instruments, Foster City, CA) amplifier, filtered at 2 kHz by the built-in filter of the amplifier, and stored on the computer. Capacitative transients were canceled at 10 MHz, and their values were obtained directly, together with the series-resistance values from the settings of the Axopatch 1D amplifier. Series-resistance compensation between 40 and 80% was applied. Leak corrections were performed using a P/N protocol. Command pulses were delivered at 20 sec intervals. Soft, soda-lime capillary glass was used to make patch pipettes, which were coated with Sylgard (Dow Corning, Midland, MI) and had resistances of 1.8-3.5 MOmega when filled with internal solution. Extracellular buffer solution for whole-cell voltage-clamp experiments was composed of (in mM): 160 tetraethylammonium chloride, 5 CaCl, 1 MgCl2, 10 HEPES, 10 glucose; pH was adjusted to 7.4 with TEAOH. The standard internal solution consisted of (in mM): 100 CsCl, 37 CsOH, 1 MgCl2, 10 BAPTA, 10 HEPES, 3.6 MgATP, 1 GTP, and 14 Tris2CP, and 50 U/ml-1 CPK. The pH was adjusted to 7.3 with CsOH. The osmolarity of the pipette solution was 300 mOsm/l, and the osmolarity of the extracellular solution was between 315 and 323 mOsm/l. GTP was replaced by GTP-gamma -S or by GDPbeta S in the double-pulse and tail-current experiments.

Stock solutions of U69593 (RBI, Natick, MA) and nor-BNI (RBI) were prepared in ethanol and stored at -20°C. [D-Trp8] somatostatin (SOM) stock solutions (Bachem, Belmont, CA) were kept at -80°C. Stock solutions of PTX (100 µg/ml; RBI) were prepared in water and stored at 4°C. Cells were treated with PTX at a final concentration of 200 ng/ml overnight.

RESULTS

Effects of GTP analogs

We examined the regulation of alpha 1B- and alpha 1E-based Ca channels stably expressed in HEK293 cells. Ca channels in these cells also contained stably expressed alpha 2/delta and beta 1B subunits (Williams et al., 1992; Bleakman et al., 1995). It has been shown that the presence of these ancillary subunits is essential for Ca channels to be expressed efficiently and display all of their normal properties (Williams et al., 1992; Bleakman et al., 1995). Ca currents could be elicited in these cells by step depolarizations to different potentials. Cells expressing alpha 1B produced Ca currents that had properties of typical N currents (Bleakman et al., 1995). Cells expressing alpha 1E produced currents whose properties we have described previously and which are similar to currents described in the literature as ``R'' type (Williams et al., 1994; Wheeler et al., 1995).

We wished to compare the regulation of alpha 1B and alpha 1E channels by G-proteins. To do this, we began by examining the effect of activating the G-proteins normally found in HEK293 cells directly, using the nonhydrolyzable analog of GTP, GTP-gamma -S. One interesting phenomenon was apparent immediately. Normally there was a marked increase in the amplitude of the Ca current in alpha 1B-expressing cells in the period after the pipette broke into the cell (Fig. 1A). This ``run-up'' of the current was not apparent in GTP-gamma -S-containing cells (Fig. 1B). One of the hallmarks of rapid ``membrane-delimited'' N-current inhibition is that it can be relieved by a depolarizing prepulse to high voltages (Bean, 1989; Hille, 1994). If the current observed during GTP-gamma -S perfusion represents an inhibited current, then a depolarizing prepulse should increase its amplitude. We therefore used a voltage-clamp protocol that consisted of two test pulses with or without a strong intervening depolarization to +80 mV (Fig. 1C,D). The ratio of current integrals (P2/P1) was plotted as a function of time (Fig. 2A-C). The ratio P2/P1 became larger after the prepulse (Fig. 2A). Figure 2A shows that the maximum GTP-gamma -S effect developed ~4 min after establishing whole-cell recording conditions. At this time, there was no difference in P2/P1 ratios with GDPbeta S in the pipette (Fig. 2B), consistent with the ``inhibitory'' action of GDPbeta S on G-protein function. The small difference in the P2/P1 ratios observed using 1 mM GTP in the patch pipette was presumably attributable to a small degree of basal G-protein activation under these conditions (Fig. 2C). Interestingly, the degree of ``run-up'' in alpha 1E-expressing cells was less marked than in alpha 1B-expressing cells. Although there was a trend suggesting that this small degree of run-up was also suppressed by GTP-gamma -S, this was not significant (Fig. 1E). Furthermore, in these cells, the current evoked by a test pulse after a depolarization also was actually smaller than without the prepulse (Fig. 1F). This was presumably attributable to differences in the extent of voltage-dependent inactivation between the two types of Ca channels (Williams et al., 1994; Bleakman et al., 1995). Consistent with this finding, the ``R''-type current in cerebellar granule neurons also displayed very pronounced voltage-dependent inactivation (Zhang et al., 1993). Thus, for alpha 1E currents, the value of the P2/P1 ratio after the prepulse fell below that seen without the prepulse (Fig. 2D-F). We therefore normalized the data by subtracting out the degree of voltage-dependent inactivation. This was obtained from cells perfused with GDPbeta S, in which we assumed that no G-protein-induced inhibition would be present. After this transformation, the data obtained with GTP or GTP-gamma -S in the pipette followed a trend similar to that obtained with alpha 1B-based channels (Fig. 2D-F, red data sets). Comparison of the data in Figure 2A-C with that in D-F, however, suggests that although there is some G-protein-mediated inhibition of alpha 1E channels, this is 7- to 10-fold less than that obtained with alpha 1B channels. This suggests that there is a considerable difference in the ability of G-proteins to regulate alpha 1B- and alpha 1E-based Ca channels directly.

Fig. 1. Ca currents in HEK293 cells expressing the alpha 1B and alpha 1E Ca channel subunits. A, Run-up with intracellular GTP using Ca (5 mM) as the charge carrier in HEK293 cells expressing the alpha 1B Ca channel subunit. Currents were evoked from a holding potential of -90 mV by 200 msec depolarizing pulses to +10 mV every 20 sec. B, Average normalized current with 1 mM GTP (black-square) or 0.3 mM GTP-gamma -S (black-triangle) in the patch pipette. The run-up of currents from individual experiments was normalized with respect to the first peak current obtained. The normalized values were then averaged, and the mean ± SEM was plotted (1 mM GTP, n = 11; 0.3 mM GTP-gamma -S, n = 6). C, D, Relief of GTP-gamma -S-induced inhibition of alpha 1B Ca currents by an intervening prepulse depolarization. Ca currents were evoked using a double-pulse protocol without (C, lower trace) or with (D, lower trace) a depolarizing prepulse using GTP-gamma -S (0.3 mM) in the patch pipette. The intervening depolarization increased the current amplitude during the second pulse (D, lower trace). Upper lines in C and D are the voltage templates (HP = -90 mV; TP = +10 mV; TP duration = 25 msec; prepulse depolarization potential = +80 mV; duration = 50 msec). P1 and P2 denote the current integrals during the first and second test pulses and are used as such in Figure 2. E, Characteristics of Ca current run-up in cells expressing alpha 1E subunit. Plot of averaged Ca current amplitude (mean ± SEM) in the presence of GTP-gamma -S (black-triangle, 0.3 mM; n = 15) or GTP (black-square, 1 mM; n = 6). Calculations as in B. F, Superimposed Ca2+ current traces evoked by the double-pulse voltage protocol with or without a depolarizing prepulse (1 mM GTP in the patch pipette). The Ca current following the prepulse depolarization was actually smaller than without it in the cell line expressing alpha 1E subunit.
[View Larger Version of this Image (25K GIF file)]

Fig. 2. Comparison of the effect of different GTP analogs on the alpha 1B (A-C) and alpha 1E (D-F) Ca current using the double-pulse protocol (see Fig. 1C,D). Currents were evoked every 20 sec in HEK293 cells expressing the alpha 1B subunit by applying the double-pulse voltage protocol with GTP-gamma -S (A), GDPbeta S (B), or GTP (C) in the patch pipette. The P2/P1 ratios from individual cells were calculated, and then the isochronal values were averaged during the time course of the experiments from cells in which experiments were carried out under identical conditions. The mean ± SE is plotted; black-square denotes the P2/P1 ratio without the prepulse, black-triangle denotes the ratio with the prepulse. GTP analogs were applied in the following concentrations: GTP-gamma -S (0.3 mM; n = 5); GDPbeta S (0.3 mM; n = 7); GTP (1 mM; n = 11). D-F, Effect of GTP analogs on alpha 1E Ca currents using the double-pulse protocol. Plot of the average P2/P1 ratios in the presence of GTP-gamma -S, 0.3 mM (n = 15; D), GDPbeta S, 0.3 mM (n = 7; E), and GTP, 1 mM (n = 6; F). Note that the values of P2/P1 ratios after prepulse application (black-triangle) fell below those of P2/P1 values obtained without the prepulse depolarization (black-square) (black data sets on D-F). D-F, black-triangle red data sets show P2/P1 ratios with prepulse after ``subtraction'' of voltage-dependent inactivation obtained from data with GDPbeta S in the patch pipette.
[View Larger Version of this Image (30K GIF file)]

Receptor regulation of Ca currents

The results presented above demonstrate that direct activation of G-proteins in these cell lines produces strong inhibition of alpha 1B but not alpha 1E channels. G-proteins are normally activated by ``serpentine'' receptors, and this can lead to inhibition of Ca currents in neurons. We attempted to reconstitute receptor regulation of Ca channels in HEK293 cells using endogenous and exogenous receptors. There are several examples in the literature of the regulation of Ca currents by somatostatin receptors (Ikeda and Schofield, 1989; Golard and Siegelbaum, 1993; Fujii et al., 1994; Hille, 1994), and endogenous somatostatin receptors have been reported to exist in HEK293 cells (Law et al., 1993). We found that activation of these receptors with the somatostatin analog SOM produced substantial inhibition of the Ca currents in alpha 1B-expressing cells (Fig. 3A,D). Little or no inhibition, however, was observed in cells expressing alpha 1E channels (Fig. 3C,D). Inhibition of the alpha 1B Ca current by SOM was repeatable, exhibiting modest desensitization (38.5 ± 5.5% inhibition on first application, 26.2 ± 3.8% inhibition on second; n = 6). Furthermore, inhibition of alpha 1B currents was blocked by pretreatment of cells with PTX (Fig. 3D) and was relieved partially by a depolarizing prepulse (Fig. 3B). We also observed that SOM was equally effective in inhibiting the Ca current in a second alpha 1B-expressing cell line that differed in the type of beta  subunit (beta 1C) expressed (Fig. 3D).
Fig. 3. Effect of SOM on alpha 1B-type and alpha 1E Ca currents. A, Plot of alpha 1B-type Ca current versus time showing a typical SOM (300 nM) response. Cell was depolarized from -90 HP to +10 TP every 20 sec. Inset shows Ca currents recorded before and during SOM application. B shows alpha 1B-type Ca currents evoked by the double-pulse protocol in the presence of SOM (300 nM). Decreased inhibition can be seen after the prepulse. C, Plot of alpha 1E-type Ca current versus time: SOM had little or no effect on the Ca current. D, Average responses (mean ± SEM) to SOM (300 nM) application. The number in parentheses represents the number of experiments.
[View Larger Version of this Image (19K GIF file)]

Activation of kappa  opioid receptors in some neurons has also been shown to produce inhibition of Ca currents (Lipscombe et al., 1989; Rhim and Miller, 1994). In contrast to the effects of SOM, however, addition of a selective agonist for the kappa  opioid receptor U69593 produced no inhibition of Ca currents in either the alpha 1B or alpha 1E cell line. This was consistent with the lack of kappa  opioid receptors in HEK293 cells as indicated by immunostaining or Northern blot analysis (data not shown). We therefore transiently transfected the kappa  opioid receptor into both alpha 1B- and alpha 1E-expressing cell lines. Northern blots and immunostaining indicated similar degrees of transfection into the two cell lines (not shown). After transfection, U69593 robustly inhibited the Ca current in alpha 1B- but not in alpha 1E-containing cells (Fig. 4). Inhibition by SOM was retained in these cells (data not shown). U69593-induced inhibition was blocked by nor-BNI, a specific inhibitor of kappa  opioid receptors (Fig. 4). Inhibition produced by U69593 was also partially voltage-dependent and inhibited by PTX treatment (Figs. 4, 5).

Fig. 4. Effects of the kappa  receptor-selective agonist U69593 on kappa  receptor-transfected HEK293 cells expressing alpha 1B and alpha 1E Ca channels. Average responses (mean ± SEM) to U69593 (200 nM); n = number of cells showing response to kappa  receptor agonist application (left to right, bars 1, 3, and 4) or all cells tested (bars 2 and 5). In experiments in which the blocking effect of nor-BNI was examined, cells were also transfected with the alpha  subunit of Go (see below). In the experiments examining the blocking effect of PTX (200 ng/ml overnight), cells were also transfected with the alpha -subunit of Gi2 (see text).
[View Larger Version of this Image (22K GIF file)]

Fig. 5. Effect of G-protein alpha  subunit overexpression on Ca current inhibition by kappa  receptor activation in cells expressing alpha 1B Ca channels. A-C, Plots of Ca current versus time. Insets show Ca current traces at the points indicated before and during the application of the kappa  receptor agonist U69593 (200 nM). HEK293 cells were transfected with only the kappa  receptor (A), kappa  receptor + Gialpha 1 (B), kappa  receptor + Goalpha (C), and kappa  receptor + Gialpha 3 (D). D, Superimposed control and inhibited (U69593; 200 nM) Ca current traces from a cell expressing the alpha 1B Ca channel, kappa  receptor, and Gialpha 3. The U69593 inhibition was partially relieved by a prepulse depolarization (see Fig. 2).
[View Larger Version of this Image (18K GIF file)]

It seemed possible that our inability to observe strong G-protein regulation of alpha 1E currents was attributable to the fact that HEK293 cells lacked a particular G-protein that was required for regulation of alpha 1E. We observed, however, that under normal conditions the cells expressed mRNAs for many different G-proteins, including representative members of all of the four major families (alpha i/o, alpha s, alpha q, alpha 13) (data not shown). Nevertheless, it was still possible that the actual quantities of some of these might be limiting. We therefore overexpressed several different G-protein alpha  subunits with and without the kappa  opioid receptor and examined the ability of U69593 or SOM to inhibit Ca currents in the two cell lines. Overexpression of each of the G-protein alpha  subunits was confirmed by Northern blot analysis. Overexpression of some of the G-protein alpha  subunits actually slightly enhanced the ability of U69593 to inhibit Ca currents in alpha 1B-expressing cells (Figs. 5B-D, 6A), although the effects of SOM were not altered (Fig. 6B). In cells overexpressing G-protein alpha  subunits, the effects observed were still blocked by PTX (Figs. 4, 6A) and were still partially voltage-dependent (Fig. 5D). On the other hand, no substantial effect of U69593 (Fig. 6C) on alpha 1E currents was observed, even in cells in which we overexpressed different G-protein alpha  subunits.

Fig. 6. A, Average inhibition (mean ± SEM) of alpha 1B Ca currents by U69593 (200 nM) in HEK293 cells expressing alpha 1B Ca channels, different G-protein alpha  subunits, and the kappa  opioid receptor. n denotes the number of cells showing agonist responses, except the first and last bars, where all of the responses were averaged. B, Inhibitory effects (mean ± SEM) of SOM (300 nM) in HEK293 cells expressing alpha 1B Ca channels, kappa  receptors, and various G-protein alpha  subunits. n denotes the number of responsive cells. C, Average inhibition (mean ± SEM) of the Ca current by U69593 (200 nM) in HEK293 cells expressing alpha 1E Ca channels, kappa  receptors, and different G-protein alpha  subunits. n = total number of cells.
[View Larger Version of this Image (21K GIF file)]

DISCUSSION

We have used an HEK293 cell expression system to make some initial attempts at understanding the G-protein regulation of Ca channels. The two types of Ca channels investigated in the present series of experiments, alpha 1B and alpha 1E, are both members of the family of nondihydropyridine-sensitive channels generally found in neurons and neuroendocrine cells. These alpha  subunits exhibit a high degree of homology with each other; they are ~80% homologous in those regions that have been suggested as compromising the 24 transmembrane helices. The degree of homology between the two types of channels falls to ~40%, however, in nontransmembrane-spanning regions such as the intracellular loop connecting domains 3 and 4 and the intracellular C-terminal extension (Soong et al., 1993; Schneider et al., 1994; Williams et al., 1994; Wheeler et al., 1995). Considering the high degree of overall homology between alpha 1B and alpha 1E and of these proteins with alpha 1A, it is of considerable interest to note the large difference in the ability of the two channel types to be regulated by G-proteins. The regulation of N channels that we have observed in HEK293 cells closely resembles that frequently described in neurons (Hille, 1994). Little is known as yet, however, about the normal properties and functions of the Ca channels that are formed by expression of alpha 1E, although the protein is widely expressed on the soma and dendrites of neurons throughout the brain (Williams et al., 1994; Volsen et al., 1995; Yokoyama et al., 1995). The experiments reported here suggest that direct G-protein regulation of these channels may be quite minimal. It should be noted that in our studies we found that G-proteins representative of all of the four major families were represented in HEK293 cells, and at least one member of each of these families was also overexpressed in our experiments. Thus, although it remains possible that alpha 1E channels are normally regulated through an exceedingly restricted direct G-protein pathway that we have missed, this seems unlikely. It should be noted in this regard that although N channel inhibition tends to be mediated by PTX-sensitive G-proteins, there are several examples of non-PTX-sensitive G-proteins also producing this type of direct, membrane-delimited inhibition (Hille, 1994; Zhu and Ikeda, 1994). This implies that for N channels, many types of G-protein alpha  subunits may be able to interact with the channel. Another interpretation of these observations would be that it is actually the beta /gamma subunits that produce the inhibition and that these may be released from various receptor-activated G-protein heterotrimers (Ikeda, 1996; Herlitze et al., 1996). Whichever mechanism is involved, it seems unlikely that any G-protein regulation of highly homologous alpha 1E channels would be highly selective. In our experiments, we have also tried to produce regulation of alpha 1E channels in several different ways. Thus, introduction of GTP-gamma -S into cells would activate the entire complement of HEK293 cell G-proteins (Zhou et al., 1995; Zong et al., 1995). Furthermore, we also used somatostatin receptors (Law et al., 1993; Shapiro and Hille, 1993; Reisine and Bell, 1995) and kappa  opioid receptors (Shen and Crain, 1994; Avidor-Reiss et al., 1995; Ikeda et al., 1995; Lai et al., 1995; Tallent et al., 1995), both of which can activate a wide variety of G-proteins. Interestingly, all of these manipulations produced robust inhibition of alpha 1B channels, which had the same ancillary subunit composition as the alpha 1E channels. We conclude that it is unlikely that we failed to observe regulation of alpha 1E channels because of the fact that we failed to express the correct components for observing such regulation, if it did indeed exist.

The characteristics of inhibition of alpha 1B channels observed in the present experiments are similar in many respects to those reported previously. For example, the inhibition displayed both voltage-dependent and -independent components (Bean, 1989; Hille, 1994; Diverse-Pierluissi and Dunlap, 1995; Diverse-Pierluissi et al., 1995). Whether this is attributable to activation of more than one G-protein-linked pathway, thereby producing more than one effect on the channels as suggested in the literature, is difficult to assess (Diverse-Pierluissi and Dunlap, 1995; Diverse-Pierluissi et al., 1995). Thus, HEK293 cells normally contained various G-proteins, even before the introduction of more of these molecules by transfection. Furthermore, both somatostatin and kappa  opioid receptors potentially can activate several of these G-proteins. The results, however, are also consistent with a single process. The effect of the depolarizing prepulse is assumed to lower the affinity of the interaction between the G-protein subunit(s) and the channel that allows unbinding (Boland and Bean, 1993). It is possible that rebinding of the G-protein might occur with a time course that is beyond the resolution of this study.

The present observations set some limitations on the localization of G-protein-binding to the alpha 1B channel. Thus, there is a high degree of sequence identity between alpha 1B and alpha 1E in their 24 putative membrane-spanning domains (Soong et al., 1993; Schneider et al., 1994; Williams et al., 1994; Wheeler et al., 1995). On the other hand, other areas exhibit less homology. This is particularly true of the large intracellular loop between domains 2 and 3 and the N and C termini. Furthermore, the smaller intracellular loop between domains 1 and 2 also shows considerable divergence. It is interesting to note that this small loop is the site of interaction between the Ca channel alpha  subunit and the small beta  subunit (Pragnell et al., 1994). Some studies have suggested that Ca channel beta  subunits and G-protein subunits may compete for a binding site on the Ca channel alpha 1 subunit, although it is not clear whether such an interaction is competitive or allosteric (Berrow et al., 1995; Campbell et al., 1995b; Roche et al., 1995). It has been suggested further that the alpha  subunits of Ca channels may possess GAP activity (Campbell et al., 1995a) and that the Ca channel alpha  and beta  subunits cooperate in enhancing the GTPase activity of the G-protein alpha  subunit. Such observations are interesting in light of recent studies showing that G-protein alpha  and beta /gamma subunits both bind to GIRK-1, one of the G-protein-regulated K channels (Huang et al., 1995). These studies also suggested that GIRK-1 may also possess GAP activity (Slesinger et al., 1995). It is possible that a similar arrangement also applies to Ca channels. Resolution of this problem will require elucidation of precisely which G-protein subunits bind to and regulate Ca channels.

FOOTNOTES

Received Feb. 15, 1996; revised April 18, 1996; accepted May 2, 1996.

  

This work was supported by Public Health Service Grants DA02121, MH40165, DA02575, and NS33502. We thank Drs. Michael Harpold (SIBIA Inc.) and David Lodge (Eli Lilly) for providing the cell lines and for helpful discussions. We thank Dongjun Ren for her technical help.

Correspondence should be addressed to Richard J. Miller, Department of Pharmacological and Physiological Sciences, The University of Chicago, 947 East 58th Street (MC 0926), Chicago, IL 60637.


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