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Volume 16, Number 24, Issue of December 15, 1996 pp. 7832-7840
Copyright ©1996 Society for Neuroscience

Thyroid Hormone-Responsive Genes in Developing Cerebellum Include a Novel Synaptotagmin and a hairless Homolog

Catherine C. Thompson

Department of Embryology, Carnegie Institution of Washington, Baltimore, Maryland 21210

ABSTRACT
INTRODUCTION
MATERIALS AND METHODS
RESULTS
DISCUSSION
FOOTNOTES
REFERENCES

ABSTRACT

Proper development of the mammalian CNS requires sufficient thyroid hormone; thyroid hormone deficiency during a brief perinatal period produces severe neurological defects in humans and experimental animals. Thyroid hormone exerts its effects through nuclear receptors, which modulate the transcription of downstream genes in response to hormone binding. Surprisingly, few genes that are regulated by thyroid hormone receptors in the CNS have been described. Here, I report the isolation and characterization of genes that are expressed in response to thyroid hormone in developing rat brain. One such gene (Srg1) encodes a novel protein related to synaptotagmin, a protein involved in regulating neurotransmitter release; another (hr) encodes a putative zinc finger protein related to the product of a recently identified mouse gene, hairless. Both Srg1 and hr are induced rapidly (<4 hr), suggesting that they are regulated directly by thyroid hormone. The temporal and spatial expression of both Srg1 and hr is characteristic of genes important to nervous system development. Srg1 and hr are likely part of a cascade of gene activation induced by thyroid hormone that is critical for CNS organization and development.

Key words: cerebellum; development; synaptotagmin; hairless; thyroid hormone; nuclear receptor

INTRODUCTION

Thyroid hormone is essential for proper development of the mammalian CNS. In humans, inadequate levels of thyroid hormone during a critical perinatal period lead to a complex of deficits (termed cretinism), which include severe mental retardation and cerebellar ataxia (DeLong and Adams, 1991). Restoration of thyroid hormone to physiological levels within a brief postnatal period can restore normal development; after this time, the damage caused by thyroid hormone deficiency is irreversible (Eayrs, 1968; Morreale de Escobar et al., 1983; Schwartz, 1983). Studies in the rat have revealed multiple morphological, biochemical, and behavioral abnormalities associated with low levels of thyroid hormone. Morphological defects include reduction in dendritic branching, axonal density, and synapse number (Morreale de Escobar et al., 1983; Schwartz, 1983). Reduction in overall myelination is also observed and may be caused by delayed differentiation of oligodendrocytes (Barres et al., 1994).

The effects of thyroid hormone are mediated via the action of specific nuclear receptor proteins that function as ligand-activated transcription factors (Oppenheimer, 1991; Tsai and O'Malley, 1994; Mangelsdorf et al., 1995). The finding that thyroid hormone receptors are present in the developing brain suggests that, as in other tissues, thyroid hormone in the brain exerts its effects by regulating the expression of specific genes (TH-responsive genes), yet little is known about the genes regulated by these receptors. Identification and functional characterization of target genes is critical, because such genes encode downstream effectors of receptor action. Of the many genes expressed in the developing nervous system, expression of only a few has been shown to be influenced by thyroid hormone (Farsetti et al., 1991; Munoz et al., 1991; Pipaon et al., 1992; Strait et al., 1992). Given the widespread effects of thyroid hormone deficiency on CNS development, it is likely that additional genes regulated by thyroid hormone remain to be discovered.

I have used subtractive hybridization coupled with PCR amplification to screen for TH-responsive genes expressed in rat brain within a critical developmental stage (postnatal days 10-15) and a specific region of the brain (cerebellum). Cerebellum was selected because it develops postnatally, at which time thyroid hormone is required, and striking effects of thyroid hormone deficiency on the cerebellum have been described (Nicholson and Altman, 1972a,b; Legrand, 1979). Several TH-responsive genes have been identified; two novel responsive genes with homology to classes of important regulatory proteins have been characterized. One gene (Srg1) encodes a novel protein related to synaptotagmin(s), a family of Ca2+/phospholipid-binding proteins primarily found in the brain, at least one of which has a role in neurotransmitter release (Bennett and Scheller, 1994; Sudhof, 1995). Another gene identified in this screen encodes a putative zinc finger protein, likely the rat homolog of a recently identified mouse gene, hairless (Cachon-Gonzalez et al., 1994). The first step in characterizing these genes with respect to their potential role in neural development has entailed determination of kinetics and developmental and tissue-specific patterns of expression. Based on temporal and spatial patterns of expression, both Srg1 and hr have properties of genes likely to be involved in nervous system development. The results of kinetic analysis suggest that hr and Srg1 are among the first direct-response genes for thyroid hormone identified in the nervous system.

MATERIALS AND METHODS

Animal care and treatment. Timed pregnant rats (Sprague Dawley) were obtained from Harlan Sprague Dawley (Indianapolis, IN). So that hyperthyroidism could be induced, pregnant rats were fed a low iodine diet (Purina Mills) and 0.025% methimazole (Sigma, St. Louis, MO) in their drinking water, starting at day 13 of gestation (E13) and continuing throughout the period of study. Methimazole blocks the synthesis of thyroid hormone in the mother and fetuses and, after birth, the pups (via nursing). For all experiments, the form of thyroid hormone used was L-T3 (3,5,3'-triiodothyronine; Sigma). The efficacy of methimazole treatment in reducing T3 levels was confirmed by direct measurement of serum T3 by RIA (Amersham, Arlington Heights, IL) and assessment of growth hormone (GH) RNA levels in the anterior pituitary (see Fig. 1). Hypothyroid pups received daily subcutaneous injection of either saline (control) or 0.25 µg/gm body weight of T3. Treatment with hormone was for 48 hr. Injection of saline had no effect on serum T3 or GH levels. Injection of T3 raised serum T3 to slightly hyperthyroid levels (5-10 vs 1-2 nM) and restored GH expression to approximately euthyroid levels. For experiments with adult animals, animals were 10 weeks old; hypothyroid adults were treated with methimazole continuously from E13. Equal numbers of males and females were used for all experiments.
Fig. 1. Isolation of novel thyroid hormone-responsive genes from developing rat brain. A, Influence of thyroid hormone on expression of rat growth hormone (GH) mRNA in methimazole-treated rats. RNA prepared from the pituitaries of 12-d-old rats was used for Northern analysis with a radiolabeled rGH cDNA probe. hypothyroid, Methimazole-treated; euthyroid, untreated; control, hypothyroid animals injected with saline; TH-treated, hypothyroid animals injected with thyroid hormone. Thyroid hormone treatment was for 24 hr. Top panel, Autoradiograph of Northern blot; bottom panel, ethidium bromide-stained gel. B, Northern analysis for two novel thyroid hormone-responsive genes (TRG16 and TRG37). A cDNA fragment isolated from the subtracted library is the probe; total RNA (15 µg per lane) prepared from cerebellum of hypothyroid P12 rats injected with saline or thyroid hormone for 48 hr was used. Right panel, Ethidium bromide-stained gel indicates equivalent loading and positions of 18S and 28S RNAs.
[View Larger Version of this Image (63K GIF file)]

Construction of subtractive library. A cDNA library enriched for genes upregulated by thyroid hormone was constructed via subtractive hybridization/PCR amplification essentially as described (Wang and Brown, 1991). The starting material was 2 µg of poly(A+) RNA from the cerebella of 12-d-old hypothyroid animals injected with either saline (control) or T3 (TH-treated), as described above. cDNA synthesized from RNA isolated from TH-treated and control rats was digested with AluI, ligated to specific linkers (Wang and Brown, 1991), and amplified by PCR. Amplified material from the control cDNA was digested with EcoRI to cleave the PCR primer site and biotinylated with photoprobe biotin (Vector Laboratories, Burlingame, CA). Twenty-fold excess control cDNA was hybridized with TH-treated cDNA, followed by removal of biotinylated cDNA with streptavidin to eliminate control/TH-treated hybrids as well as unhybridized control cDNA. The remaining material was PCR-amplified, and the procedure was repeated two times. The final pool of cDNA was amplified, cloned into pBluescript (Stratagene, La Jolla, CA), and transformed into bacterial strain DH5alpha . DNA was prepared from individual colonies, digested with EcoRI to excise the cDNA insert, and separated by agarose gel electrophoresis. These gels were used for Southern analysis with the final pool of cDNA as probe. Approximately 30% of the colonies gave a detectable hybridization signal; these represented the most abundant members of the library and therefore those most enriched and likely to be TH-responsive. To confirm that isolates corresponded to TH-responsive genes, I used the cDNA inserts from positive clones as probes for Northern analysis of RNA prepared from control and TH-treated hypothyroid P12 rats. Fragments from six different TH-responsive genes were isolated. The two genes chosen for further study on the basis of their kinetics and expression patterns (TRG16 and TRG37) are described; the other genes have not been characterized further.

RNA preparation/Northern analysis. Animals were killed by decapitation, and tissues were removed and frozen in dry ice. RNA was prepared with acid/phenol as described (Chomczynski and Sacchi, 1987). Poly(A+) RNA was selected by using oligo-dT-coupled magnetic beads (Promega, Madison, WI) or oligo-dT cellulose chromatography (Sambrook et al., 1989). For Northern analysis, RNA samples were separated on 1% agarose/formaldehyde gels and transferred to nitrocellulose. Radiolabeled probes were prepared by random priming (Feinberg and Vogelstein, 1984). Unless specified otherwise, the probe for hr was a 2.2 HindIII fragment from nucleotide position 2245 to 3771 of the rat hr cDNA; the probe for Srg1 was from nucleotide position 253 to 2071 of the cDNA. Ethidium bromide staining was used to assure that equivalent amounts of RNA were loaded per lane; filters were checked after blotting to confirm equivalent transfer of RNA. The probe for detecting TRalpha 1 was an HphI-XbaI fragment from rbeA12 (Thompson et al., 1987) corresponding to the unique C terminus of TRalpha 1 and included 250 bp of 3' untranslated region. The probe for detecting TRbeta 1 was a HindIII-XbaI fragment from rc-erbAbeta 1 (Murray et al., 1988; kindly provided by H. Towle, University of Minnesota) corresponding to the unique N terminus of TRbeta 1. The probe for rat growth hormone was an EcoRI fragment from the rGH cDNA (kindly provided by R. Evans, Salk Institute).

Isolation of full-length cDNA clones. An oligo-dT-primed cDNA library was constructed in lambda UNI-ZAP (Stratagene) under conditions recommended by the manufacturer. The starting material was RNA from the cerebella of hypothyroid P12 animals treated with thyroid hormone for 48 hr. The library was screened by using cDNA fragments isolated from the subtractive library as probes.

For TRG16 (Srg1), 10 different cDNAs were isolated; two contained the entire open reading frame. Six isolates differed by a deletion of 57 nucleotides between positions 196 and 253. Five isolates were 800 bp longer at the 3' end, likely attributable to internal priming of a stretch of consecutive T residues beginning at nucleotide 2063. For TRG37 (hr), four different cDNAs were isolated; the largest of these was 3.5 kb. To isolate cDNA encompassing the rest of the rat hr gene, I used a specific oligonucleotide corresponding to position 1898-1916 in the cDNA (CT102) to reverse transcribe RNA from cerebellum, followed by 5' RACE with a nested primer (CT103; Rapid Amplification of cDNA Ends, Life Technologies, Bethesda, MD) (Frohman et al., 1988; Loh et al., 1989). Specificity of the amplified cDNA was confirmed by hybridization with a nested oligonucleotide (CT104). This resulted in the isolation of an additional 1.4 kb cDNA. Specific oligonucleotides were synthesized to position 630-650 in the cDNA (CT152) and used for reverse transcription, followed by 5' RACE (CT153) to isolate an additional 500 bp. Specificity of the amplified cDNA was confirmed by hybridization with a nested oligonucleotide (CT154). Primers for 5' RACE (nucleotide positions correspond to the full-length cDNA) included the following: CT102, 5'-CAGATGTATCCTCAAGTCTG (nt 1898-1916); CT103, 5'-GATTCCCGGAGCCGAATCCT (nt 1877-1897); CT104, 5'-GGCCCTCTTTGCTCCTCTTGTTGCTGTGCC (nt 1837-1866); CT154, 5'-TAGGCACAGTGCCCCATGGT (nt 577-596); CT153, 5'-CCTCCAAAACCCAACAGGTTC (nt 600-621); and CT152, 5'-AGCCAGGAGTCTGGGGCGCTC (nt 630-650). Both 5' RACE products hybridized to the same size RNA as the original cDNA (data not shown). The combined size of the overlapping cDNAs was 5.3 kb and contained the entire open reading frame.

The complete nucleotide sequences for Srg1 and hr were determined on both strands by the chain termination method (Sequenase/USB, Cleveland, OH) with either subcloned restriction fragments or specific oligonucleotides. The complete nucleotide and amino acid sequences have been submitted to GenBank under accession numbers U71293[GenBank] (hr) and U71294[GenBank] (Srg1).

Cell culture. GH1 (rat pituitary) cells were obtained from ATCC (Rockville, MD). Cells were grown in DMEM supplemented with 10% fetal calf serum. For induction experiments, serum was depleted of thyroid and steroid hormones by treatment with AG-1-X8 resin (Bio-Rad, Hercules, CA) and charcoal (Sigma), as described (Samuels et al., 1979). Cells were grown for 2 d in hormone-depleted media; then thyroid hormone (T3) was added to 10-7 M, and cycloheximide was added to 10 µg/ml. After treatment for 16 hr, cells were harvested, and RNA was prepared as described.

In situ hybridization. Animals (P14) were killed by decapitation, brains were removed, and cerebellum and brain stem were separated from midbrain and forebrain and frozen with dry ice/ethanol in O.C.T. compound embedding medium (Baxter, McGaw Park, IL). Sections (10 µm) were prepared with a cryostat and transferred to slides (ProbeOn Plus, Fisher Scientific, Pittsburgh, PA). Sections were stored at -20°C. 35S-labeled cRNA probes were prepared by synthesis with either T3 or T7 RNA polymerase in the presence of 35S-UTP (New England Nuclear, Boston, MA). Hybridization conditions were as described (Simmons et al., 1989; Simerly and Young, 1991), except that fixation was for 1 hr and proteinase K treatment was for 15 min. Hybridization with sense strand cRNA was used as a negative control. Autoradiographic images were obtained by direct exposure of hybridized sections to x-ray film (Amersham Hyperfilm beta -max). Probe for hr was from nucleotide 2703 to 5186 of the cDNA; probe for Srg1 spanned nucleotides 783-1574 of the cDNA.

RESULTS

Isolation of TH-responsive genes from developing rat brain

To identify genes regulated by thyroid hormone in developing brain, I treated pregnant rats with methimazole to induce hypothyroidism in their pups (see Materials and Methods). Because the critical period for thyroid hormone action in rat brain is between postnatal days 10 and 15, on postnatal day 12 (P12) one-half of the methimazole-treated pups were injected with thyroid hormone (TH-treated), the other one-half with saline (control). Treatment with thyroid hormone was for 48 hr. To demonstrate the efficacy of this paradigm, I monitored the level of mRNA for a known responsive gene (growth hormone). Growth hormone mRNA was greatly reduced in the pituitaries of hypothyroid animals and was restored to euthyroid (normal) levels after thyroid hormone treatment (Fig. 1A). RNA isolated from the cerebella of control and TH-treated animals was the starting material for construction of a cDNA library enriched for genes upregulated by thyroid hormone. The procedure for preparing the library is based on subtractive hybridization coupled with PCR amplification (Wang and Brown, 1991; see Materials and Methods). Identification of positive clones (cDNA fragments originating from TH-responsive genes) was accomplished by using the isolated fragments as probes for Northern analysis. Fragments that detected higher expression in RNA from cerebella of TH-treated animals relative to control animals were judged to be from TH-responsive genes. Fragments corresponding to six different TH-responsive genes were isolated. Two genes were chosen for further study on the basis of their kinetics and expression patterns (described below); Northern analysis for these genes (TRG16 and TRG37) is shown in Figure 1B. Low basal level expression is observed for both TRG16 and TRG37 in hypothyroid cerebellum. Expression of TRG16 is induced approximately threefold by thyroid hormone; induction of TRG37 is ~10-fold.

Identification of novel TH-responsive genes

To obtain full-length cDNAs for TRG16 and TRG37, I constructed a cDNA library, which was then screened using the isolated cDNA fragments as probes. For TRG37, I used library screening and 5' RACE to isolate a combined cDNA of 5.3 kb. The open reading frame (ORF) contained within this sequence encodes a predicted protein of 1207 amino acids. The only recognizable structural motif in the primary amino acid sequence is a single putative zinc finger (Fig. 2A). A search of the database (GenBank) revealed that this putative protein is similar to that encoded by a recently identified mouse gene, hairless (hr). The TRG37 ORF is 94% identical to the ORF of the mouse hairless gene, suggesting that TRG37 is the rat homolog of hairless. Therefore, TRG37 was renamed hr.
Fig. 2. TRG37 encodes a putative zinc finger protein related to hairless; TRG16 encodes a novel synaptotagmin. A, Amino acid sequence of TRG37 (94% amino acid identity with ORF from mouse hairless gene). Cysteine residues potentially involved in formation of a zinc finger are underlined. B, Amino acid sequence of TRG16 (Srg1). Protein kinase C-related (C2) domains are underlined; putative transmembrane domain is indicated by dashed underline. C, Schematic representation of Srg1 structure. Cross-hatched boxes represent C2 domains; Srg1 shows between 26 and 37% amino acid identity in the region encompassing the C2 domains with synaptotagmins I-VIII (19-31% in C2-A; 33-42% in C2-B). Black box (TM) at the N terminus indicates putative transmembrane domain. The complete nucleotide sequences have been submitted to GenBank under accession numbers U71293[GenBank] (hr) and U71294[GenBank] (Srg1).
[View Larger Version of this Image (46K GIF file)]

For TRG16, I isolated multiple cDNAs that contained an ORF encoding a predicted protein of 421 amino acids (Fig. 2B). A search of the database (GenBank) revealed that this putative protein is most similar to synaptotagmin(s). Synaptotagmins share a common structure that includes a C-terminal region consisting of two domains related to the regulatory domain of protein kinase C (C2 domains; Fig. 2C). The eight members of the synaptotagmin family are related within this region (41-89% amino acid identity with synaptotagmin I), but they do not show significant amino acid identity outside this region. TRG16 has between 26 and 37% amino acid identity with the different synaptotagmins in this C-terminal region. Also characteristic of synaptotagmins, the TRG16 ORF lacks a signal sequence but has a hydrophobic region near the N terminus, a putative transmembrane domain. Because of this homology with synaptotagmins, TRG16 has been termed Srg1 (Synaptotagmin related gene 1).

Srg1 and hr respond rapidly to thyroid hormone

Genes that respond to hormones or other activators often are divided into the broad categories of primary (direct) response genes and secondary (indirect) response genes. Primary or direct response genes are defined as those that respond rapidly and without the need for protein synthesis, indicating that they are direct targets of regulation by a particular transcriptional activator. The induction of most previously described TH-responsive genes is blocked by protein synthesis inhibitors, suggesting that they are secondary response genes (Kanamori and Brown, 1992).

To determine whether Srg1 and hr are induced directly by thyroid hormone, I examined the kinetics of regulation. Hypothyroid neonatal (P12) rats were injected with thyroid hormone and sacrificed at various times after treatment. Northern analysis of RNA prepared from cerebellum shows that both Srg1 and hr are rapidly upregulated (Fig. 3). Induction of Srg1 occurs within 2 hr of treatment, reaching maximal levels by 4-8 hr. hr is induced within 4 hr, reaching maximal levels by 8-12 hr. The rapid response of Srg1 and hr suggests that these genes may be direct targets of thyroid hormone action.

Fig. 3. Srg1 and hr respond rapidly to thyroid hormone. So that the kinetics of response to thyroid hormone could be determined, 12-d-old hypothyroid rats were injected with saline (-) or thyroid hormone (+) and killed at various times after injection. Total RNA (10 µg per lane) prepared from cerebellum was used for Northern analysis with 32P-labeled cDNA probes for hr (top) or Srg1 (bottom). (-), Control; (+), TH-treated; hr, hr after injection. Two independent experiments gave the same results. B, hr responds to thyroid hormone in the absence of protein synthesis. GH1 (rat pituitary) cells were grown in hormone-free media (-) for 2 d. T3 was added to 10-7 M (TH); cycloheximide (CHX) was added to 10 µg/ml for 16 hr. Total RNA (15 µg per lane) was used for Northern analysis.
[View Larger Version of this Image (68K GIF file)]

Cultured cells were used to determine whether induction of hr by thyroid hormone (TH) could be blocked by inhibition of protein synthesis with cycloheximide (CHX); Srg1 was not analyzed, because it was not detected in any of the cell lines tested. GH1 (rat pituitary) cells grown in the absence of TH were treated with TH, CHX, or both for 16 hr. Northern analysis shows that TH induces a fourfold increase in hr expression and that this increase is resistant to cycloheximide treatment (Fig. 3). Simultaneous treatment with CHX and TH showed increased expression relative to CHX or TH alone, likely attributable to stabilization of the message by CHX. Hybridization of the same blot with a probe for growth hormone (GH) showed that, as expected, induction of GH by TH was partially blocked by cycloheximide (data not shown). The induction of hr expression by thyroid hormone in the absence of protein synthesis shows that hr responds directly to thyroid hormone.

Expression patterns of Srg1 and hr are consistent with regulation by thyroid hormone during development

To establish whether Srg1 and hr are regulated by thyroid hormone during normal development, I determined the expression pattern of both genes and compared it with that of thyroid hormone receptors (TRalpha 1 and TRbeta 1). TH-responsive genes likely to function in neural development should be expressed at the time known to be important for the effect of thyroid hormone (postnatal days 10-15) and when thyroid hormone receptors are present. Northern analysis of RNA isolated from embryonic and postnatal brain shows that Srg1 and hr both are expressed at high levels shortly after birth, reaching a peak between postnatal days 14 and 21 (Fig. 4). Consistent with previous observations (Strait et al., 1990; Forrest et al., 1991; Mellström et al., 1991; Bradley et al., 1992), expression of TRbeta 1 rises at birth, immediately preceding expression of Srg1 and hr. TRalpha 1 is expressed prenatally and remains at high levels after birth (Forrest et al., 1991; Mellström et al., 1991; Bradley et al., 1992). Therefore, both Srg1 and hr are expressed at the appropriate time in normal development, both with respect to the critical time frame for the effect of thyroid hormone and the presence of thyroid hormone receptors.
Fig. 4. Developmental expression of hr and Srg1 in brain. Northern analysis of RNA prepared from the brains of embryonic and neonatal rats. Parallel blots (10 µg of total RNA per lane) were hybridized with 32P-labeled cDNA probes from hr, Srg1, TRbeta 1, TRalpha 1 (top to bottom). E, Day of gestation; E22/P0, birth; P, days after birth.
[View Larger Version of this Image (71K GIF file)]

Srg1 and hr show tissue-restricted expression

To determine whether expression of Srg1 and hr is restricted to the CNS, I performed Northern analysis of RNA isolated from various rat tissues. Expression of Srg1 was not detected outside the nervous system in either neonatal (Fig. 5A) or adult animals (data not shown). Neonatal expression of hr is abundant in brain and skin and is also detected at low levels in other tissues, including gut, lung, muscle, and pituitary (Fig. 5A). The expression pattern is the same in adult tissues, except for an additional RNA of ~3.5 kb detected in brain (see Fig. 4). In contrast to brain, the hairless gene is not regulated by thyroid hormone in skin (Fig. 5B).
Fig. 5. Tissue distribution of hr and Srg1. A, RNA was prepared from various tissues collected from euthyroid animals on postnatal day 12. Total RNA (15 µg per lane) was used for Northern analysis with 32P-labeled cDNA probes from hr (top) or Srg1 (bottom). Spleen RNA is somewhat degraded; all other RNAs are equivalent, as assessed by ethidium bromide staining. B, hr is regulated by thyroid hormone in brain, but not in skin. Shown is Northern analysis of RNA from cerebellum (CEREB) and skin (SKIN) of hypothyroid and euthyroid neonatal (P14) rats. Eu, Euthyroid; H, hypothyroid.
[View Larger Version of this Image (45K GIF file)]

Srg1 and hr are expressed specifically in the internal granule cell layer of the cerebellum

To examine more precisely the expression of Srg1 and hr in the cerebellum, I used in situ hybridization. Hybridization of coronal sections from P14 rats showed that both Srg1 and hr were expressed in the internal granule cell layer (IGL) of the cerebellum (Fig. 6). During maturation of the cerebellum, cells migrate from the external granule cell layer (EGL) to the IGL, a region where the cell bodies of maturing neurons are found (Altman, 1972a-c; Rakic, 1972). The absence of expression in the EGL and presence in the IGL suggest that Srg1 and hr are expressed only in differentiated cells. To determine whether injection of hypothyroid animals with thyroid hormone restored this expression pattern, I used in situ hybridization of sections from hypothyroid animals injected with either saline or thyroid hormone (Fig. 6D). For both Srg1 and hr, expression induced in the cerebellum of thyroid hormone-treated animals was indistinguishable from expression in euthyroid animals. Little or no expression above background was detected in hypothyroid animals. Induction of Srg1 by thyroid hormone was greater in cerebellum than in more rostral brain regions (data not shown).
Fig. 6. Expression of Srg1 and hr in neonatal cerebellum. Shown is in situ hybridization of coronal sections from cerebellum of P14 rat. Shown are results from Srg1; signal in the cerebellum with an hr probe is comparable (see B). A, Thionine counterstain (after hybridization); B, hybridization with antisense probe; C, hybridization with sense probe. EGL, External granule cell layer; IGL, internal granule cell layer. Sections (10 µm) of frozen tissue were hybridized with 35S-labeled cRNA probes. B, C, Shown are photomicrographs of an autoradiographic image of the hybridized section exposed directly to x-ray film. D, Expression induced by thyroid hormone injection is indistinguishable from euthyroid expression in neonatal cerebellum. In situ hybridization of coronal sections from cerebella of P14 rats using a cRNA probe from Srg1 (top) or hr (bottom). Eu, Euthyroid; H, hypothyroid; H + TH, hypothyroid treated with thyroid hormone for 48 hr. Sections were hybridized simultaneously with the same probe preparation. Shown are photomicrographs of the autoradiographic image of the hybridized section exposed directly to x-ray film.
[View Larger Version of this Image (70K GIF file)]

Although this strategy initially focused on the cerebellum, thyroid hormone affects many different regions of the brain, suggesting that at least some TH-responsive genes are expressed outside the cerebellum. In situ hybridization was used to examine expression of Srg1 and hr in other regions of the brain. hr is broadly expressed at low levels throughout the forebrain and midbrain (data not shown), whereas Srg1 shows a more restricted expression pattern (Fig. 7). High levels of Srg1 expression are detected in several regions of the brain, including the cortex, caudate putamen, hippocampus, thalamus, piriform cortex, and amygdala. Previous studies have shown that these regions express other synaptotagmins as well (Ullrich et al., 1994). This is a common feature of synaptotagmins; different family members are often coexpressed. Because Srg1 is regulated by thyroid hormone, this raises the question of whether synaptotagmins are regulated by thyroid hormone as well. Northern analysis of RNA from the brains of hypothyroid and normal animals showed that two other synaptotagmins (syt I and syt III) are not regulated by thyroid hormone (data not shown). Thus, regulation by thyroid hormone is not a feature common to all members of the synaptotagmin family and may be a property unique to Srg1.

Fig. 7. Expression of Srg1 in neonatal rat brain. Shown is in situ hybridization of coronal sections from P14 rat brain. Sections are shown rostral (A) to caudal (D). Hybridization with an antisense 35S-cRNA probe from Srg1 is shown; sense probe gave no detectable signal. Shown are photomicrographs of an autoradiographic image of the hybridized section exposed directly to x-ray film. AAA, Anterior amygdaloid area; ACB, nucleus accumbens; BMA, basal medial amygdala; CA1, CA3, regions of hippocampus; CoAp, cortical nucleus amygdala, posterior part; CP, caudate putamen; DG, dentate gyrus; IsoCtx, isocortex; LA, lateral nucleus; LHA, lateral hypothalamic area; MeA, medial nucleus amygdala; OT, olfactory tubercle; PIR, piriform area; thal, thalamus; VB, ventrobasal complex, thalamus; ZI, zona incerta.
[View Larger Version of this Image (87K GIF file)]

DISCUSSION

Novel TH-responsive genes isolated from neonatal rat brain

This study describes the identification and initial characterization of genes regulated by thyroid hormone in developing rat brain. Srg1 and hr represent the most rapidly regulated (<4 hr) TH-responsive genes identified in the mammalian nervous system; to date, hr is also the most highly induced (>10-fold). hr does not require protein synthesis for upregulation and thus is a direct target of thyroid hormone receptors. Srg1 is activated with similar kinetics, so it is likely that Srg1 is a direct response gene as well. Direct response genes are of particular interest because such genes likely constitute the first step in the genetic program responsible for TH-mediated aspects of neural development.

Srg1 and hr have been shown to be regulated by thyroid hormone in this experimental system; it is likely that they are regulated by thyroid hormone during normal development as well. Both genes are highly expressed within the first 2 weeks after birth, a time known to be crucial for the effect of thyroid hormone on development. Temporal expression of Srg1 and hr also is correlated with the presence of thyroid hormone receptors. As shown here and consistent with previous studies examining receptor mRNA levels in the brain (Strait et al., 1990; Forrest et al., 1991; Mellstrom et al., 1991; Bradley et al., 1992), expression of Srg1 and hr immediately follows that of TRbeta 1. TRalpha 1 is present at this time as well, but unlike TRbeta 1 it is also present at high levels prenatally. The timing of Srg1 and hr expression suggests that regulation of these genes is attributable either to the increased levels of thyroid hormone present after birth or specific regulation by TRbeta 1. These alternatives can be distinguished by examining expression of these genes after thyroid hormone levels are raised precociously.

In situ hybridization analysis of Srg1 and hr showed that both are expressed in the IGL, but not the EGL, of the cerebellum. In the course of cerebellar development, neuroblasts residing in the EGL undergo terminal mitosis. The resulting postmitotic cells migrate inward and ultimately populate the IGL (Altman, 1972a-c; Rakic, 1972; Hatten and Heintz, 1995). Both genes appear to be expressed in terminally differentiated cerebellar neurons and not in neuronal precursors. Thyroid hormone has been implicated in promoting neuronal differentiation in the cerebellum (Nicholson and Altman, 1972b) and has been shown to be involved in differentiation of oligodendrocytes (Barres et al., 1994). Based on these observations, an attractive hypothesis is that the function of hr is in establishment or maintenance of a differentiated state. The postulated function of Srg1 as a component of synapses (see below) makes it unlikely that Srg1 is involved directly in differentiation.

Srg1, a novel gene related to synaptotagmin

One of the genes isolated in this screen (Srg1) encodes a novel protein related to synaptotagmin(s). Synaptotagmins comprise a family of Ca2+/phospholipid-binding proteins found in the brain (Perin et al., 1990; Geppert et al., 1991; Hilbush and Morgan, 1994; Mizuta et al., 1994; Li et al., 1995). Among the eight related genes that have been identified (syt I-VIII), four also are expressed outside the nervous system (Mizuta et al., 1994; Li et al., 1995). The functional significance of multiple synaptotagmins is not clear. Mice lacking a functional syt I gene are deficient in the fast component of Ca2+-mediated neurotransmitter release at hippocampal synapses (Geppert et al., 1994). Syt III is expressed in the same hippocampal neurons as syt I but does not substitute for syt I, suggesting that synaptotagmins do not have redundant functions (Ullrich et al., 1994).

Srg1 and synaptotagmins share a common structure that includes a C-terminal region consisting of two domains related to the regulatory region of protein kinase C (C2 domains). The N-terminal C2 domain (C2-A) of multiple synaptotagmins has been shown to bind Ca2+ and phospholipids (Davletov and Sudhof, 1993; Li et al., 1995). The C-terminal C2 domain (C2-B) has been shown to bind a clathrin-coated vesicle protein complex (AP-2) and syntaxins (Zhang et al., 1994; Li et al., 1995); all known synaptotagmins bind AP-2 and syntaxin. Srg1 is related to synaptotagmins more in the C2-B domain (33-42% amino acid identity) than in the C2-A domain (19-31%) and is most related to syt IV and syt VI, forms that do not bind Ca2+ and phospholipids. Based on structural similarity, Srg1 is likely to bind AP-2 and syntaxins but may not bind Ca2+ and phospholipids. Further studies will determine whether Srg1 can be classified functionally with synaptotagmins.

Regulation by thyroid hormone suggests that Srg1 may have unique functions among the synaptotagmins; other synaptotagmins have been tested (syt I and syt III) and are not induced by thyroid hormone. The brains of thyroid hormone-deficient animals are estimated to have approximately one-half the number of synapses of euthyroid animals (Eayrs, 1968; Nicholson and Altman, 1972a). Overall reduction in the number of interneuronal connections has been proposed to be the cause of behavioral changes in experimental animals and mental retardation in humans (Eayrs, 1968; Schwartz, 1983). Therefore, it is tempting to speculate that Srg1 may have a role in synaptogenesis or synaptic remodeling, processes in which synaptotagmins previously were not implicated. The temporal and spatial pattern of expression of Srg1 in the cerebellum is consistent with this hypothesis. Srg1 is expressed in the cerebellar IGL; migration of cells from the EGL to the IGL immediately precedes synapse formation by these cells.

hr, the rat homolog of hairless

This screen also identified a gene, hairless, not previously known to be TH-responsive. hr is regulated by thyroid hormone in brain, yet its expression is not influenced by thyroid hormone in skin. This unusual tissue-specific regulation may indicate a functional difference in the action of hr in brain and skin.

The murine hairless locus was identified as a spontaneous mutation caused by the insertion of an endogenous retrovirus between exons 6 and 7 (Stoye et al., 1988; Cachon-Gonzalez et al., 1994). The most prominent phenotype of homozygous mutant (hr/hr) mice is that ~2 weeks after birth they experience progressive hair loss. After their coat is shed completely (3-4 weeks of age), they remain bald. These animals show increased sensitivity to UV and chemically induced skin cancer (Poland et al., 1982; de Gruijl and Forbes, 1995). In both skin and brain, homozygous mutant hairless mice have ~20-fold less hr mRNA than heterozygotes (my unpublished observations). Although reduction of hr mRNA and, presumably, protein results in an obvious skin phenotype, the phenotype in the brain has not been determined. Generation of hairless null mutant mice will provide a useful tool for determining the in vivo function of hr in the nervous system; future studies will also examine whether mice homozygous for existing hr alleles have brain defects resembling those of hypothyroid animals.

The protein encoded by hr has a putative zinc finger domain. Although the zinc finger motif usually occurs in multiple copies, several large proteins in a variety of species contain a single zinc finger (Friden and Schimmel, 1987; Evans and Hollenberg, 1988; Fu and Marzluf, 1990; Kudla et al., 1990; Tang et al., 1994), many of which have been implicated in transcriptional regulation. hr has a cluster of six cysteines with novel spacing that is conserved among mouse, rat, and human (Cachon-Gonzalez et al., 1994; my unpublished observations). In addition, hr is related to a gene with testes-specific expression identified in rat (TSGA; Höög et al., 1991). The proteins encoded by hr and TSGA show 33% amino acid identity over a 260 amino acid region at their respective C termini. The putative zinc finger domain lies outside this 260 amino acid region, yet the arrangement of six cysteine residues is conserved (shown below; underlined residues are conserved between hr and TSGA).

-<UNL>C</UNL>-S-R-<UNL>C</UNL>-H-H-G-L-<UNL>F</UNL>-<UNL>N</UNL>-T-<UNL>H</UNL>-<UNL>W</UNL>-R-<UNL>C</UNL>S-H-C-<UNL>S</UNL>-H-R-L-<UNL>C</UNL>-<UNL>V</UNL>-A-<UNL>C</UNL>-G-<UNL>R</UNL>-I-

This configuration may be functionally significant; hr and TSGA may be representative of a new class of zinc finger proteins. Given the functional roles of other zinc finger proteins, the zinc finger domain of hr is likely to have a role in nucleic acid binding and/or protein-protein interactions.

Conclusions

Thyroid hormone affects neural development by initiating a finely tuned program of gene expression. The results presented here suggest that this thyroid hormone-induced genetic program includes Srg1 and hr, making these genes excellent candidates for performing important functions in neural development. The success of this screen in identifying TH-responsive genes opens new avenues for studying the molecular effects of thyroid hormone on the mammalian CNS.

FOOTNOTES

Received July 26, 1996; revised Sept. 24, 1996; accepted Sept. 26, 1996.

  

This work was supported by the John Merck Fund at Community Trust, the American Cancer Society, and National Institutes of Health (National Institute of Diabetes and Digestive and Kidney Diseases). I thank L. Buckbinder, A. Lanahan, and Z. Wang for advice on subtractive hybridization; R. Simerly for advice on in situ hybridization; D. Brown and A. Kanamori for helpful discussions; O. Martin for technical assistance; A. Pinder for oligonucleotide synthesis; M. Sepanski for sectioning; H. Towle for the rat TRbeta cDNA; and R. Evans for the rat growth hormone cDNA. I am grateful to S. Dymecki, C.-M. Fan, P. Rorth, and G. Seydoux for critical comments on this manuscript.

Correspondence should be addressed to Dr. Catherine C. Thompson, Department of Embryology, Carnegie Institution of Washington, 115 West University Parkway, Baltimore, MD 21210.


REFERENCES

  • Altman J (1972a) Postnatal development of the cerebellar cortex in the rat. I. The external germinal layer and the transitional molecular layer. J Comp Neurol 145:353-398 . [Web of Science][Medline]
  • Altman J (1972b) Postnatal development of the cerebellar cortex in the rat. II. The external germinal layer and the transitional molecular layer. J Comp Neurol 145:399-464 . [Web of Science][Medline]
  • Altman J (1972c) Postnatal development of the cerebellar cortex in the rat. III. The external germinal layer and the transitional molecular layer. J Comp Neurol 145:465-514 . [Web of Science][Medline]
  • Barres BA, Lazar MA, Raff MC (1994) A novel role for thyroid hormone, glucocorticoids, and retinoic acid in timing oligodendrocyte development. Development (Camb) 120:1097-1108 . [Abstract]
  • Bennett MK, Scheller RH (1994) A molecular description of synaptic vesicle membrane trafficking. Annu Rev Biochem 63:63-100 . [Web of Science][Medline]
  • Bradley DJ, Towle HC, Young WS (1992) Spatial and temporal expression of alpha  and beta  thyroid hormone receptor mRNAs, including the beta 2 subtype, in the developing mammalian nervous system. J Neurosci 12:2288-2302 . [Abstract]
  • Cachon-Gonzalez MB, Fenner S, Coffin JM, Moran C, Best S, Stoye JP (1994) Structure and expression of the hairless gene of mice. Proc Natl Acad Sci USA 91:7717-7721 . [Abstract/Free Full Text]
  • Chomczynski P, Sacchi N (1987) Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal Biochem 162:156-159 . [Web of Science][Medline]
  • Davletov BA, Sudhof TC (1993) A single C2-domain from synaptotagmin I is sufficient for high affinity Ca2+/phospholipid-binding. J Biol Chem 268:26386-26390 . [Abstract/Free Full Text]
  • de Gruijl R, Forbes PD (1995) UV-induced skin cancer in a hairless mouse model. BioEssays 17:651-660. [Web of Science][Medline]
  • DeLong GR, Adams RD (1991) The neuromuscular system and brain in hypothyroidism. In: The thyroid (Braverman, LE, Utiger, RD, eds) , p. 1027. New York: Lippincott.
  • Eayrs JT (1968) In: Endocrinology and human behavior (Michael RP, ed), pp 331-363. . London: Oxford UP.
  • Evans RM, Hollenberg SM (1988) Zinc fingers: gilt by association. Cell 52:1-3 . [Web of Science][Medline]
  • Farsetti A, Mitsuhashi T, Desvergne B, Robbins J, Nikodem VM (1991) Molecular basis of thyroid hormone regulation of myelin basic protein gene expression in rodent brain. J Biol Chem 266:23266-23232.
  • Feinberg AP, Vogelstein B (1984) A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity. Anal Biochem 132:6-13.
  • Forrest D, Hallböök F, Persson H, Vennströmm B (1991) Distinct functions for thyroid hormone receptors alpha  and beta  in brain development indicated by differential expression of receptor genes. EMBO J 10:269-275 . [Web of Science][Medline]
  • Friden P, Schimmel P (1987) LEU3 of Saccharomyces cerevisiae encodes a factor for control of RNA levels of a group of leucine-specific genes. Mol Cell Biol 7:2708-2717 . [Abstract/Free Full Text]
  • Frohman MA, Dush MK, Martin GR (1988) Rapid production of full-length cDNAs from rare transcripts: amplification using a single gene-specific oligonucleotide primer. Proc Natl Acad Sci USA 85:8998-9002 . [Abstract/Free Full Text]
  • Fu Y-H, Marzluf GA (1990) Nit-2, the major nitrogen regulatory gene of Neurospora crassa, encodes a protein with a putative zinc finger DNA-binding domain. Mol Cell Biol 10:1056-1065 . [Abstract/Free Full Text]
  • Geppert M, Archer III, BT, Sudhof TC (1991) Synaptotagmin II: a novel differentially distributed form of synaptotagmin. J Biol Chem 266:13548-13552 . [Abstract/Free Full Text]
  • Geppert M, Goda Y, Hammer RE, Li C, Rosahl TW, Stevens CF, Sudhof TC (1994) Synaptotagmin I: a major Ca2+ sensor for transmitter release at central synapses. Cell 79:717-727 . [Web of Science][Medline]
  • Hatten ME, Heintz N (1995) Mechanisms of neural patterning and specification in the developing cerebellum. Annu Rev Neurosci 18:385-408 . [Web of Science][Medline]
  • Hilbush BS, Morgan JI (1994) A third synaptotagmin gene, Syt3, in the mouse. Proc Natl Acad Sci USA 91:8195-8199 . [Abstract/Free Full Text]
  • Höög C, Schalling M, Grunder-Brundell E, Daneholt B (1991) Analysis of a murine male germ cell-specific transcript that encodes a putative zinc finger protein. Mol Reprod Dev 30:173-181 . [Web of Science][Medline]
  • Kanamori A, Brown DD (1992) The regulation of thyroid hormone receptor beta  genes by thyroid hormone in Xenopus laevis. J Biol Chem 267:739-745 . [Abstract/Free Full Text]
  • Kudla B, Caddick MX, Langdon T, Martinez-Rossi NM, Bennett CF, Sibley S, Davies RW, Arst HN Jr (1990) The regulatory gene areA mediating nitrogen metabolite repression in Aspergillus nidulans. Mutations affecting specificity of gene activation alter a loop residue of a putative zinc finger. EMBO J 9:1355-1364 . [Web of Science][Medline]
  • Legrand J (1979) Morphogenetic actions of thyroid hormones. Trends Neurosci 2:234-236. [Web of Science]
  • Li C, Ullrich B, Zhang JZ, Anderson RGW, Brose N, Sudhof TC (1995) Ca2+-dependent and independent activities of neural and non-neural synaptotagmins. Nature 375:594-599 . [Medline]
  • Loh EY, Elliot JF, Cwirla S, Lanier LL, Davis MM (1989) Polymerase chain reaction with single-sided specificity: analysis of T cell receptor delta  chain. Science 243:217-220 . [Abstract/Free Full Text]
  • Mangelsdorf DJ, Thummel C, Beato M, Herrlich P, Schutz G, Umesono K, Blumberg B, Kastner P, Mark M, Chambon P, Evans RM (1995) The nuclear receptor superfamily: the second decade. Cell 83:835-839 . [Web of Science][Medline]
  • Mellström B, Naranjo JR, Santos A, Gonzalez AM, Bernal J (1991) Independent expression of the alpha  and beta  c-erbA genes in developing rat brain. Mol Endocrinol 5:1339-1350 . [Abstract/Free Full Text]
  • Mizuta M, Inagaki N, Nemoto Y, Matsukura S, Takahashi M, Seino S (1994) Synaptotagmin III is a novel isoform of rat synaptotagmin expressed in endocrine and neuronal cells. J Biol Chem 269:11675-11678 . [Abstract/Free Full Text]
  • Morreale de Escobar G, Ruiz-Marcos A, Escobar del Rey F (1983) Thyroid hormone and the developing brain. In: Congenital hypothyroidism (Dussault, JH, Walker, P, eds) , p. 85. New York: Dekker.
  • Munoz A, Rodriguez-Pena A, Perez-Castillo A, Ferreiro B, Sutcliffe JG, Bernal J (1991) Effects of neonatal hypothyroidism on rat brain gene expression. Mol Endocrinol 5:273-280 . [Abstract/Free Full Text]
  • Murray MB, Zilz ND, McCreary NL, MacDonald MJ, Towle HC (1988) Isolation and characterization of rat cDNA clones for two distinct thyroid hormone receptors. J Biol Chem 263:12770-12777 . [Abstract/Free Full Text]
  • Nicholson JL, Altman J (1972a) Synaptogenesis in the rat cerebellum: effects of early hypo- and hyperthyroidism. Science 176:530-532 . [Abstract/Free Full Text]
  • Nicholson JL, Altman J (1972b) The effects of early hypo- and hyperthyroidism on the development of the rat cerebellar cortex (I and II). Brain Res 44:13-36 . [Web of Science][Medline]
  • Oppenheimer JH (1991) Thyroid hormone action at the molecular level. In: The thyroid (Braverman, LE, Utiger, RD, eds) , p. 204. New York: Lippincott.
  • Perin MS, Fried VA, Mignery GA, Jahn R, Sudhof TC (1990) Phospholipid binding by a synaptic vesicle protein homologous to the regulatory region of protein kinase C. Nature 345:260-261 . [Medline]
  • Pipaon C, Santos A, Perez-Castillo A (1992) Thyroid hormone up-regulates NGFI-A gene expression in rat brain during development. J Biol Chem 267:21-23 . [Abstract/Free Full Text]
  • Poland A, Palen D, Glover E (1982) Tumour production by TCDD in skin of HRS/J mice. Nature 300:271-273 . [Medline]
  • Rakic P (1972) Extrinsic cytological determinants of basket and stellate cell dendritic pattern in the cerebellar molecular layer. J Comp Neurol 146:335-354 . [Web of Science][Medline]
  • Sambrook J, Fritsch EF, Maniatis T (1989) In: Molecular cloning: a laboratory manual, pp. 7.26-7.29. . Cold Spring Harbor, NY: Cold Spring Harbor Laboratory.
  • Samuels HH, Stanley F, Casanova J (1979) Depletion of L-3,5,3'-triiodothyronine and L-thyroxine in euthyroid calf serum for use in cell culture studies of the action of thyroid hormone. Endocrinology 105:80-85 . [Abstract/Free Full Text]
  • Schwartz HL (1983) Effect of thyroid hormone on growth and development. In: Molecular basis of thyroid hormone action (Oppenheimer, JH, Samuels, HH, eds) , p. 413. New York: Academic.
  • Simerly RB, Young BJ (1991) Regulation of estrogen receptor mRNA in rat hypothalamus by sex steroid hormones. Mol Endocrinol 5:424-432 . [Abstract/Free Full Text]
  • Simmons DM, Arriza JL, Swanson LW (1989) A complete protocol for in situ hybridization of messenger RNAs in brain and other tissues with radiolabeled single-stranded RNA probes. J Histotechnol 12:169-181.
  • Stoye JP, Fenner S, Greenoak GE, Moran C, Coffin JM (1988) Role of endogenous retroviruses as mutagens: the hairless mutation of mice. Cell 54:383-391 . [Web of Science][Medline]
  • Strait KA, Schwartz HL, Perez-Castillo A, Oppenheimer JH (1990) Relationship of c-erbA mRNA content to tissue triiodothyronine nuclear binding capacity and function in developing and adult rats. J Biol Chem 265:10514-10521 . [Abstract/Free Full Text]
  • Strait KA, Zou L, Oppenheimer JH (1992) beta 1 isoform-specific regulation of a triiodothyronine-induced gene during cerebellar development. Mol Endocrinol 6:1874-1880 . [Abstract/Free Full Text]
  • Sudhof TC (1995) The synaptic vesicle cycle: a cascade of protein-protein interactions. Nature 375:645-653 . [Medline]
  • Tang CSL, Buenos A, Russell P (1994) Ntf1+ encodes a 6-cysteine zinc finger-containing transcription factor that regulates the nmt1 promoter in fission yeast. J Biol Chem 269:11921-11926. [Abstract/Free Full Text]
  • Thompson CC, Weinberger CW, Lebo R, Evans RM (1987) Identification of a novel thyroid hormone receptor expressed in the mammalian central nervous system. Science 237:1610-1614 . [Abstract/Free Full Text]
  • Tsai M-J, O'Malley BW (1994) Molecular mechanisms of action of steroid/thyroid receptor superfamily members. Annu Rev Biochem 63:451-486 . [Web of Science][Medline]
  • Ullrich B, Li C, Zhang JZ, McMahon H, Anderson RGW, Geppert M, Sudhof TC (1994) Functional properties of multiple synaptotagmins in brain. Neuron 13:1281-1291 . [Web of Science][Medline]
  • Wang Z, Brown DD (1991) A gene expression screen. Proc Natl Acad Sci USA 88:11505-11509 . [Abstract/Free Full Text]
  • Zhang JZ, Davletov BA, Sudhof TC, Anderson RGW (1994) Synaptotagmin I is a high affinity receptor for clathrin AP-2: implications for membrane recycling. Cell 78:751-760 . [Web of Science][Medline]
Copyright ©1996 Society for Neuroscience   0270-6474/1996/167832-09$05.00/0



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Home page
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M. Fukuda, E. Kanno, and K. Mikoshiba
Conserved N-terminal Cysteine Motif Is Essential for Homo- and Heterodimer Formation of Synaptotagmins III, V, VI, and X
J. Biol. Chem., October 29, 1999; 274(44): 31421 - 31427.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
M. Fukuda and K. Mikoshiba
A Novel Alternatively Spliced Variant of Synaptotagmin VI Lacking a Transmembrane Domain. IMPLICATIONS FOR DISTINCT FUNCTIONS OF THE TWO ISOFORMS
J. Biol. Chem., October 29, 1999; 274(44): 31428 - 31434.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
N. Koibuchi, H. Fukuda, and W. W. Chin
Promoter-Specific Regulation of the Brain-Derived Neurotropic Factor Gene by Thyroid Hormone in the Developing Rat Cerebellum
Endocrinology, September 1, 1999; 140(9): 3955 - 3961.
[Abstract] [Full Text]

Home page
 J. Biol. Chem.Home page
R. J. Denver, L. Ouellet, D. Furling, A. Kobayashi, Y. Fujii-Kuriyama, and J. Puymirat
Basic Transcription Element-binding Protein (BTEB) Is a Thyroid Hormone-regulated Gene in the Developing Central Nervous System. EVIDENCE FOR A ROLE IN NEURITE OUTGROWTH
J. Biol. Chem., August 13, 1999; 274(33): 23128 - 23134.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Pathol.Home page
A. A. Panteleyev, N. V. Botchkareva, J. P. Sundberg, A. M. Christiano, and R. Paus
The Role of the Hairless (hr) Gene in the Regulation of Hair Follicle Catagen Transformation
Am. J. Pathol., July 1, 1999; 155(1): 159 - 171.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
S. Butz, R. Fernandez-Chacon, F. Schmitz, R. Jahn, and T. C. Sudhof
The Subcellular Localizations of Atypical Synaptotagmins III and VI. SYNAPTOTAGMIN III IS ENRICHED IN SYNAPSES AND SYNAPTIC PLASMA MEMBRANES BUT NOT IN SYNAPTIC VESICLES
J. Biol. Chem., June 25, 1999; 274(26): 18290 - 18296.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
K. Ibata, M. Fukuda, and K. Mikoshiba
Inositol 1,3,4,5-Tetrakisphosphate Binding Activities of Neuronal and Non-neuronal Synaptotagmins. IDENTIFICATION OF CONSERVED AMINO ACID SUBSTITUTIONS THAT ABOLISH INOSITOL 1,3,4,5-TETRAKISPHOSPHATE BINDING TO SYNAPTOTAGMINS III, V, AND X
J. Biol. Chem., May 15, 1998; 273(20): 12267 - 12273.
[Abstract] [Full Text] [PDF]

Home page
 ScienceHome page
W. Ahmad, M. Faiyaz ul Haque, V. Brancolini, H. C. Tsou, S. ul Haque, H. Lam, V. M. Aita, J. Owen, M. deBlaquiere, J. Frank, et al.
Alopecia Universalis Associated with a Mutation in the Human hairless Gene
Science, January 30, 1998; 279(5351): 720 - 724.
[Abstract] [Full Text]

Home page
 Proc. Natl. Acad. Sci. USAHome page
A. Guadano-Ferraz, M. J. Obregon, D. L. St. Germain, and J. Bernal
The type 2 iodothyronine deiodinase is expressed primarily in glial cells in the neonatal rat brain
PNAS, September 16, 1997; 94(19): 10391 - 10396.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
C. C. Thompson and M. C. Bottcher
The product of a thyroid hormone-responsive gene interacts with thyroid hormone receptors
PNAS, August 5, 1997; 94(16): 8527 - 8532.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
R. J. Denver, S. Pavgi, and Y.-B. Shi
Thyroid Hormone-dependent Gene Expression Program for Xenopus Neural Development
J. Biol. Chem., March 28, 1997; 272(13): 8179 - 8188.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
Y. Itoh, T. Esaki, M. Kaneshige, H. Suzuki, M. Cook, L. Sokoloff, S.-Y. Cheng, and J. Nunez
Brain glucose utilization in mice with a targeted mutation in the thyroid hormone alpha or beta receptor gene
PNAS, August 14, 2001; 98(17): 9913 - 9918.
[Abstract] [Full Text] [PDF]

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