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Volume 17, Number 1, Issue of January 1, 1997 pp. 83-90
Copyright ©1997 Society for Neuroscience

Pituitary Adenylate Cyclase-Activating Polypeptide (PACAP-38) Protects Cerebellar Granule Neurons from Apoptosis by Activating the Mitogen-Activated Protein Kinase (MAP Kinase) Pathway

Martin Villalba, Joël Bockaert, and Laurent Journot

Centre National de la Recherche Scientifique (CNRS), Unité Propre de Recherche 9023, Centre CNRS-Institut National de la Santé et de la Recherche Médicale de Pharmacologie-Endocrinologie, F-34094 Montpellier Cedex 05, France

ABSTRACT
INTRODUCTION
MATERIALS AND METHODS
RESULTS
DISCUSSION
FOOTNOTES
REFERENCES

ABSTRACT

Pituitary adenylate cyclase-activating polypeptides (PACAP-27 and PACAP-38) are neuropeptides of the vasoactive intestinal polypeptide (VIP)/secretin/glucagon family. PACAP receptors are expressed in different brain regions, including cerebellum. We used primary culture of rat cerebellar granule neurons to study the effect of PACAP-38 on apoptosis induced by potassium deprivation. We demonstrated that PACAP-38 increased survival of cerebellar neurons in a dose-dependent manner by decreasing the extent of apoptosis estimated by DNA fragmentation. PACAP-38 induced activation of the extracellular signal-regulated kinase (ERK)-type of mitogen-activated protein (MAP) kinase through a cAMP-dependent pathway. PD98059, an inhibitor of MEK (MAP kinase kinase), completely abolished the antiapoptotic effect of PACAP-38, suggesting that MAP kinase pathway activation is necessary for PACAP-38 action.

Key words: PACAP; cerebellum; apoptosis; MAP kinase; cAMP; PD 98059

INTRODUCTION

Cerebellar granule cells are among the most abundant neuronal population in the mammalian CNS. During the first few weeks of postnatal life, there is a well documented cell loss in the maturing granule cell layer of the cerebellum (Landis and Sidman, 1978). Cerebellar granule cells undergo apoptosis between postnatal days 5 and 9, whereas cell loss between the third and fifth postnatal weeks is not associated with DNA fragmentation (Wood et al., 1993). In vitro culture of newborn rat cerebellar neurons provided a good model to study neuronal apoptosis because of the high degree of cellular homogeneity (Marini and Paul, 1992). Cerebellar granule cells survive and differentiate in vitro in the presence of depolarizing concentrations of KCl (25-30 mM) without additional need for neurotrophic factors (Gallo et al., 1990). The mechanism of action of KCl remains obscure so far, but, generally, it is believed that an increase in intracellular Ca2+ concentration (D'Mello et al., 1993; Yan et al., 1994; Galli et al., 1995) and mitogen-activated protein (MAP) kinase activation (Rosen et al., 1995) induced by depolarization are involved. In the presence of a normal concentration of KCl (5-10 mM), cerebellar granule cells undergo apoptosis, which is inhibited by different categories of molecules: (1) forskolin (D'Mello et al., 1993) and cholera toxin (Yan et al., 1995a), which raise cAMP levels; (2) IGF-1 (D'Mello et al., 1993), which activates a tyrosine kinase receptor; and (3) agonists of muscarinic cholinergic receptors (Yan et al., 1995b) and metabotropic glutamate receptors (Copani et al., 1995), which stimulate phospholipase C. The effect of cAMP is of particular interest because it was also demonstrated in other neuronal systems, such as sympathetic and sensory neurons (Rydel and Greene, 1988), dopamine neurons (Mena et al., 1995), and developing septal cholinergic neurons (Kew et al., 1996). The mechanism underlying the cAMP antiapoptotic effect is not well understood; however, it was suggested that the MAP kinase pathway is involved (Kew et al., 1996). Although cAMP inhibits the MAP kinase cascade in some cell lines (Burgering et al., 1993; Cook and McCormick, 1993; Graves et al., 1993; Sevetson et al., 1993; Wu et al., 1993) and has no effect in rat sympathetic neurons (Virdee and Tolkovsky, 1995), it stimulates MAP kinase cascade in other cell lines, including PC12 (Faure et al., 1994; Frödin et al., 1994). MAP kinase activation also has been involved in the protection of PC12 cells from NGF withdrawal-induced apoptosis (Xia et al., 1995).

Modulation of granule cell loss by physiological agents has not been described carefully. Evidence for the presence of pituitary adenylate cyclase-activating polypeptides (PACAP) and PACAP receptor in the cerebellum is compelling and suggests a physiological role for PACAP in cerebellum development (Lam et al., 1990; Cauvin et al., 1991; Basille et al., 1993, 1995; Hashimoto et al., 1993; Spengler et al., 1993; Favit et al., 1995). PACAPs are neuropeptides of the vasoactive intestinal polypeptide (VIP)/secretin/glucagon family, which are named according to their amino acid number: PACAP-27 and PACAP-38. PACAP-27 corresponds to the 27 N-terminal amino acids of PACAP-38 and displays 68% homology with VIP. Two classes of PACAP receptors have been described with respect to their pharmacological properties: type I PACAP receptors bind PACAP-27 and -38 two orders of magnitude more potently than VIP, whereas type II PACAP receptors do not discriminate between PACAP-27, -38, and VIP. At present three genes encoding PACAP/VIP receptors have been cloned. PACAP1-R corresponds to type I binding sites, whereas PACAP/VIP1-R and PACAP/VIP2-R correspond to type II PACAP receptors. No VIP-specific receptor has been cloned yet. PACAP-38 modulates the release of several pituitary hormones (Miyata et al., 1989) and of catecholamines from the adrenal gland (Watanabe et al., 1992; Isokobe et al., 1993). In addition, PACAP-38 promotes neurite outgrowth in PC12 cells (Deutsch and Sun, 1992; Hernandez et al., 1995) and NB-OK neuroblastoma (Deutsch et al., 1993), stimulates neuritogenesis and survival of cultured rat sympathetic neuroblasts (Pincus et al., 1990; DiCicco-Bloom and Deutsch, 1992), and prevents natural neuronal cell death in chick embryo and HIV gp120-induced cell death in hippocampal cultures (Arimura et al., 1994). Because of the demonstrated presence of PACAP-38 in the cerebellum and the neurotrophic and neuroprotective activity of PACAP-38 in other systems and because PACAP-38 stimulates cAMP production, we tested PACAP-38 as a modulator of apoptosis in primary culture of cerebellar granule cells.

MATERIALS AND METHODS

Materials. PACAP was obtained from Neosystem (Strasbourg, France), [gamma -32P]ATP and [2-3H]adenine from Isotopchim, Biotrak MAP Kinase Enzyme assay kit from Amersham (Arlington Heights, IL), IGF-1 from Bachem AG (Torrence, CA), Rp-cAMP and Sp-cAMP from RBI, antibody against phospho-ERK from New England Biolabs (Beverly, MA), H89 from Calbiochem (Lucerne, Switzerland), BMEM and BMEM-Glutamax-I from Life Technologies (Gaithersburg, MD), and Taq DNA polymerase from Eurobio. PD98059 was a generous gift from Dr. Alan R. Saltiel (Parke-Davis Pharmaceutical Research). All other reagents, unless otherwise indicated, were from Sigma (St. Louis, MO).

Culture of cerebellar granule cells. Rat cerebellar granule neurons were prepared from 8-d-old Wistar rat pups, as previously described (Levi et al., 1984). Briefly, freshly dissected cerebella were incubated with 0.25 mg/ml trypsin for 10 min at 37°C. Trypsin inhibitor (0.5 mg/ml) and 0.08 mg/ml DNase were added to stop the reaction. Digested cerebella were centrifuged and disrupted mechanically with a Pasteur pipette in the presence of DNase and trypsin inhibitor. Cells were seeded at a density of 0.25 × 106 cells/cm2 in Falcon dishes coated with poly-L-lysine in BMEM supplemented with 10% fetal calf serum (FCS), 10 U of penicillin, 10 µg of streptomycin, 30 mM KCl, 10 mM HEPES, pH 7.4, 17 mM glucose, and 2 mM glutamine. After 24 hr, 10 µM cytosine arabinoside was added to the culture medium. Cultures were used after 7 d in vitro (DIV).

Neuronal viability. Viable granule neurons were quantified as previously described (Didier et al., 1990). Briefly, 7 DIV cultures were washed twice with HK medium (BMEM-glutamax-I supplemented with 30 mM KCl, 10 mM HEPES, pH 7.4, and 17 mM glucose) and cultivated for 48 hr in HK or LK medium (BMEM-glutamax-I supplemented with 10 mM KCl, 10 mM HEPES, pH 7.4, and 17 mM glucose) with or without the different drugs. For control cultures in serum-containing medium, the cells were washed twice with HK medium and refed with culture medium used throughout the culture to avoid the neurotoxic effect of fresh serum-containing medium (Yan et al., 1994). Cultures were exposed to 15 µg/ml fluorescein diacetate (FDA) for 10 min at 37°C. The medium was aspirated, and lysis buffer (15 mM Tris-HCl, pH 7.4, and 1% SDS) was added. After 15 min, the supernatant was collected, and the amount of fluorescein was determined with a spectrofluorometer.

Quantification of DNA fragmentation. Cultures (7 DIV) were washed twice with HK medium. HK or LK medium with or without the different drugs was added for 24 hr. In experiments in which antagonists were tested, neurons were preincubated for 1 hr with the antagonists in serum-containing medium to allow loading of the cells. Neurons (5 × 106) were trypsinized, scraped, and collected by centrifugation. Three hundred microliters of lysis buffer (0.5% Triton X-100, 20 mM EDTA, and 5 mM Tris-HCl, pH 7.4) were added, and incubation was continued for 20 min at 4°C. Cells were centrifuged at 27,000 × g for 15 min. Pellets (nuclei plus intact chromatin) were incubated with 300 µl of lysis buffer plus 100 µg of proteinase K. Proteinase K (60 µg) was added to the supernatant (soluble DNA). Samples were incubated for 2 hr at 60°C and then overnight at 37°C with gentle shaking. The DNA was extracted with phenol-chloroform. Soluble DNA (200 µl) or nonsoluble DNA (50 µl) was incubated in TNE buffer (10 mM Tris-HCl, pH 7.4, 200 mM NaCl, and 1 mM EDTA) with 0.5 µg/ml of Hoechst 33258. Fluorescence was measured with a spectrofluorometer (excitation 356 nm; emission 492 nm). Similar results were obtained with the Cell Death Detection ELISA kit from Boehringer Mannheim (Cat. No. 1544 675; Mannheim, Germany).

DNA laddering. DNA extraction was performed as described above. After phenol-chloroform extraction, the DNA was precipitated with ethanol, resuspended in TE buffer (10 mM Tris-HCl, pH 8.0, and 1 mM EDTA) supplemented with 2 µg/ml RNase A, and incubated overnight at 37°C. Samples were electrophoresed through a 1.2% agarose gel. DNA was visualized by ethidium bromide staining.

Photomicrographs. Cultures (7 DIV) were incubated for 48 hr as indicated above with the different drugs. Cells were incubated with FDA as indicated above and photographed with a phase-contrast microscope or with a fluorescence microscope using the fluorescein filter after three washes with PBS. For propidium iodide staining, 7 DIV cultures were incubated for 48 hr as indicated above with the different drugs. At the end of the incubation period, cells were fixed with 3% paraformaldehyde in PBS and then permeabilized with 0.2% Triton X-100 in PBS for 15 min at room temperature. Cells were incubated overnight with 50 µg/ml propidium iodide at 4°C. After three washes with PBS, coverslips were mounted with Mowiol and examined by fluorescence microscopy using a rhodamine filter.

Extracellular signal-regulated kinase (ERK) activity. ERK activity was determined by the Biotrak p42/p44 MAP kinase enzyme assay kit as recommended by the manufacturer (Amersham). Briefly, 7 DIV cultures were washed twice with HK medium. HK or LK medium with or without the different drugs was added for 10 min at 37°C. In experiments in which antagonists were tested, neurons were preincubated for 1 hr with the antagonists in serum-containing medium to allow loading of the cells. The incubations were terminated by aspiration, and the cells were lysed immediately in lysis buffer containing (in mM): Tris-HCl 10, pH 7.4, NaCl 150, EGTA 2, DTT 2, orthovanadate 1, and PMSF 1 with 10 µg/ml leupeptin, 10 µg/ml aprotinin, and 0.2% Triton X-100. Cell extract (15 µl) was mixed with 10 µl of substrate buffer containing an ERK-specific peptide substrate, and the reaction was started by adding 5 µl of [gamma 32P]-ATP. After 30 min at 30°C, the reaction was terminated by adding 10 µl of stop reagent containing orthophosphoric acid. Then 30 µl was pipetted onto the center of paper disks, followed by washing twice in 75 mM orthophosphoric acid for 2 min and twice in water for 2 min. Disks containing phosphorylated peptide were counted on a scintillation counter.

Detection of phospho-ERKs. After incubation for 10 min at 37°C as described above for determination of ERK activity, 7 DIV cultures were lysed in Laemmli buffer. Samples were electrophoresed through a 10% acrylamide gel and blotted on a nitrocellulose membrane. Membranes were treated with a phospho-specific MAPK antibody (1:1000; New England Biolabs) and developed with the Phototope-Star Western Blot Detection kit (New England Biolabs). Then membranes were stripped with stripping solution (100 mM Tris-HCl, pH 6.7, 2% SDS, and 100 mM beta -mercaptoethanol), incubated with anti-ERK1 and anti-ERK2 antibodies (1:400 each; Santa Cruz Biotechnology, Tebu, France), and developed with the Renaissance Western detection kit (Dupont NEN, Boston, MA).

Determination of cAMP levels. Cultures (7 DIV) were washed twice with HK medium and incubated for 2 hr in the presence of [2-3H]adenine in HK medium. Cells were incubated in the same medium supplemented with 0.5 mM 3-isobutyl-1-methylxanthine (IBMX) for 15 min at 37°C. Various concentrations of PACAP or VIP were added for 15 min. The medium was aspirated, and the reaction was stopped by the addition of 1 ml of 5% trichloroacetic acid (TCA) at 4°C. Cells then were scraped and centrifuged. cAMP levels in the supernatant were measured as previously described (Salomon et al., 1974).

RT-PCR experiments. Total RNA was prepared from 7 DIV cultures using standard techniques (Chomczynski and Sacchi, 1987). RT-PCR reaction was performed as described (Rawlings et al., 1995). Single-stranded cDNA was synthesized from total RNA with the following protocol. One microliter of total RNA (3 µg/µl) was preincubated with 3 µl of a hexamer random primer mixture (100 µM) and 22.5 µl of H2O at 70°C for 10 min and then rapidly chilled on ice. To this reaction were added 1 µl of RNasin (40 U/µl), 10 µl of 5× RT buffer, 5 µl of dithiothreitol (100 mM), and 2.5 µl of deoxyribonucleotides (dNTP; 10 mM of each), and the mixture was incubated at 45°C for 2 min. Finally, 5 µl of Moloney murine leukemia virus reverse transcriptase (MoMuLVRT; 200 U/µl) was added to give a final volume of 50 µl; the reaction was incubated at 45°C for 60 min and then terminated by placing it on ice. The template produced from the RT reaction was amplified using one of three sets of primers, dependent on the PACAP/VIP receptor we wished to identify. For all three receptors we used primers that flank the putative third intracellular loop of the receptor. For PACAP1-R, the two primers used were PAC1-FL (5'-TTTCATCGGCATCATCATCATCATCCTT-3') and PAC1-VK (5'-CCTTCCAGCTCCTC-CATTTCCTCTT-3'), which would be expected to produce PCR product sizes of 280 base pairs (bp) for the "short" variant, 361 bp for the "hop2" variant, 364 bp for the "hip" or "hop1" variants, 445 for the "hip-hop2" variant and 448 bp for the "hip-hop1" variant (Spengler et al., 1993). For PACAP/VIP1-R, the primers used were VIP1-AI (5'-GCCCCCATCCTCCTCTCCATC-3') and VIP1-EL (5'-TCCGCCTGCACCTCACCATTG-3'), which should give a PCR product of 299 bp (Ishihara et al., 1992). The PACAP/VIP2-R primers used were VIP2-AE (5'-ATGGATAGCAACTCGCCTTTCTTTAG-3') and VIP2-QL (5'-GGAAGGAACCAACACATAACTCAAACAG-3'), yielding a predicted PCR product 325 bp in length (Lutz et al., 1993). For the PCR reaction, 29 µl of H2O, 5 µl of 10× PCR buffer, 4 µl of MgCl2 (25 mM), 1 µl of dNTP (10 mM of each), 0.5 µl of Taq DNA polymerase (5 U/µl), 0.25 µl of both the relevant oligonucleotide primers (50 µM), and 5 µl of the relevant RT products (or, for the controls, 5 µl of the RT reaction in which reverse transcriptase had been omitted) were added to each tube to give a final volume of 50 µl. The PCR reaction was run on a GeneAmp PCR system 9600 (Perkin-Elmer/Cetus, Norwalk, CT) at 94°C for 1 min, followed by 35 cycles of 94°C for 30 sec, 58°C for 30 sec, 72°C for 45 sec, and then a final cycle of 72°C for 420 sec. Because the same primer pair is used to amplify all variants of the PACAP1-R, the relative abundance of the PCR products is a qualitative index of the abundance of the corresponding transcripts. This point was verified by mixing various proportions of different plasmids containing, each, a different insert encoding a different variant (data not shown).

RESULTS

Cultures (7 DIV) were washed and incubated with HK medium or LK medium with or without the different drugs, as indicated in Materials and Methods. Viable cells were visualized by staining with FDA (Fig. 1A). As previously reported (D'Mello et al., 1993), decreasing potassium concentration induced a large decrease in the number of cells stained by FDA, and this effect was abrogated by IGF-1 and partially abolished by compounds raising cAMP levels, such as forskolin (Fig. 1A). Interestingly, the effect of forskolin was reproduced fully by PACAP-38 (Fig. 1A). For more quantitative results, we measured cell survival via the FDA conversion technique (Fig. 1B). The decrease in potassium concentration induced a large decrease in FDA conversion, which was protected partially by forskolin or PACAP-38. Interestingly, protection by PACAP-38 was dose-dependent with a maximal effect at 100 nM and an EC50 of 5 nM (Fig. 1C).
Fig. 1. PACAP-38 increases survival of cerebellar granule neurons. A, Seven-day-old neurons were deprived of serum and maintained for 48 hr in serum-containing medium (FCS), HK medium (HK), LK medium (LK), or LK medium supplemented with 25 ng/ml IGF-1 (IGF), 100 nM PACAP38 (P38), or 10 µM forskolin (FK). Then cultures were incubated with FDA and photographed by phase-contrast and fluorescence microscopy. Magnification bar, 50 µm. B, Survival was determined by the FDA method (see Materials and Methods). Results were expressed as the percentage of total fluorescein production in sister cultures treated with HK medium. Data are the mean ± SEM of three independent experiments performed in triplicate. *p < 0.0005 with Student's t test, as compared with (a) HK and (b) LK. C, Dose-response curve of PACAP-38-induced increase in FDA conversion. Data are the mean ± SEM of three independent experiments performed in triplicate.
[View Larger Version of this Image (67K GIF file)]

A decrease in potassium concentration was reported previously to induce apoptosis in cerebellar granule cells (D'Mello et al., 1993; Yan et al., 1994). Therefore, staining with propidium iodide was performed after permeabilization of the cells. Changing from serum-containing medium to HK medium did not induce apoptosis (Fig. 2A). In contrast, the presence of numerous nuclei displaying fragmented or condensed chromatin, a characteristic feature of apoptotic nuclei (Earnshaw, 1995), revealed apoptosis in LK medium (Fig. 2A). Interestingly, PACAP-38 (100 nM) and forskolin (10 µM) efficiently protected neurons from apoptosis, because the number of apoptotic nuclei was reduced (Fig. 2A). The biochemical hallmark of apoptosis is nuclear DNA fragmentation into oligonucleosomal fragments, which can be visualized as a DNA ladder by agarose gel electrophoresis of soluble DNA (Wyllie, 1980; Hockenberry et al., 1990). Using this method, we confirmed that potassium deprivation induced apoptosis in cerebellar granule cell cultures and showed that PACAP-38 (100 nM) or forskolin (10 µM) decreased the intensity of DNA laddering. High KCl concentration completely blocked laddering (Fig. 2B). By comparing the amount of fragmented DNA in the presence of PACAP-38 with different amounts of fragmented DNA from LK condition, we estimated the protective effect of PACAP-38 to 60% (Fig. 2B). To have a more quantitative assessment of DNA laddering, we developed a protocol to quantify the proportion of fragmented DNA, as described in Materials and Methods. PACAP-38 protected cerebellar granule neurons from apoptosis in a dose-dependent manner, with a maximal effect ~100 nM (Fig. 2C).

Fig. 2. PACAP-38 protects cerebellar granule neurons from apoptosis. A, Changes in nuclear morphology. Seven-day-old neurons were deprived of serum and maintained for 24 hr in HK medium (HK), LK medium (LK), or LK medium supplemented with either 100 nM PACAP-38 (P38) or 10 µM forskolin (FK). Control cultures were kept in serum-containing medium (FCS). Magnification bar, 10 µm. B, PACAP decreased fragmentation of DNA from cerebellar granule cells induced by LK medium. Cultures were incubated as in A. Soluble DNA was extracted, electrophoresed, and visualized with ethidium bromide staining. To illustrate quantitatively the extent of protection from DNA laddering by PACAP or forskolin, we loaded a different amount of soluble DNA obtained from one plate incubated in LK medium alone (100-20%). Data are representative of three independent experiments. C, Dose-response curve of PACAP-38-induced decrease in DNA fragmentation. Seven-day-old neurons were deprived of serum and maintained for 24 hr in LK medium supplemented with different PACAP-38 concentrations. Soluble and nonsoluble DNA was isolated and quantified as described in Materials and Methods. Data are the mean ± SEM of three independent experiments.
[View Larger Version of this Image (46K GIF file)]

It is noteworthy that the switch from serum-containing medium used throughout the culture to HK medium also induced some cell death (data not shown). However, this death was essentially necrotic, because no DNA laddering and no chromatin condensation were shown in HK medium (Fig. 2B). Furthermore, neither forskolin nor PACAP-38 had any protective effect in HK medium, as compared with HK medium alone, when FDA conversion was measured (data not shown). This indicates that the cells protected by forskolin or PACAP-38 were those dying by apoptosis because of potassium deprivation and not those dying by necrosis because of serum withdrawal.

To identify the PACAP receptor(s) involved, we performed RT-PCR with primers specific for the different PACAP/VIP receptor subtypes and splice variants. We demonstrated the expression of PACAP1-R and PACAP/VIP1-R (Fig. 3A). The products obtained with primers specific for the PACAP1-R indicated that variants with one cassette (hip or hop) were expressed preferentially and that the short variant was expressed at much lower levels. Using restriction enzymes that are specific for the hip (AvaII) or hop (Bpu1102 I) variants (Rawlings et al., 1995), we identified the one cassette band as PACAP1-R hop (data not shown). Conclusively, cerebellar granule cells mainly express PACAP1-R hop mRNA. In addition, we performed pharmacological characterization of the expressed PACAP/VIP receptor(s) (Fig. 3B). Stimulation of cAMP production by PACAP and VIP indicated the presence of type I PACAP receptor, which is compatible with RT-PCR experiments. On the other hand, the potency of VIP to stimulate cAMP production was low, indicating that no type II PACAP receptor (PACAP/VIP1-R or PACAP/VIP2-R) was expressed significantly in contrast to what was anticipated from RT-PCR experiments.

Fig. 3. A, Cerebellar granule neurons express predominantly PACAP1-R with one cassette. Seven-day-old neurons were washed twice with HK medium, and total RNA was isolated. After reverse transcription (RT+) and PCR amplification, DNA was electrophoresed and visualized with ethidium bromide. Reverse transcriptase was omitted in control experiments (RT-). The predicted fragment size was 280 bp for PACAP1-R s (short variant), 361 bp (hop2) or 364 bp (hip or hop1) for a single cassette insert, and 445 bp (hip-hop2) or 448 bp (hip-hop1) for double insert, 299 bp for PACAP/VIP1-R, and 325 bp for PACAP/VIP2-R. Data are representative of two independent experiments. B, PACAP- or VIP-stimulated cAMP production in cerebellar granule cells. Neurons were incubated for 15 min at 37°C in LK medium containing the indicated concentrations of PACAP-38 (open circles), PACAP-27 (open squares), or VIP (filled triangles). Data are expressed as the percentage of cAMP production induced by 20 µM forskolin. Data are the mean ± SEM of three independent experiments performed in triplicate.
[View Larger Version of this Image (35K GIF file)]

Because activation of the MAP kinase pathway has been involved in protection from apoptosis and because cAMP has been shown to stimulate MAP kinase activity in some experimental systems, we measured PACAP-stimulated ERK activity. PACAP-38 stimulated ERK activity in a dose-dependent manner with a maximal effect at 100 nM (Fig. 4A). We demonstrated that tetrodotoxin (TTX) did not block PACAP-induced stimulation of MAP kinase activity (Fig. 4B), suggesting that the effect of PACAP was not mediated by the release of neurotransmitters or neurotrophic factors. Rp-cAMP and H89, two inhibitors of PKA, and PD98059, an inhibitor of MEK, blocked stimulation of ERK activity by PACAP-38 and forskolin (Fig. 4C). We extended our analysis by performing Western blots with anti-phospho-ERK antibodies. We demonstrated that ERK2 was more abundant than ERK1 and that PACAP-38 induced phosphorylation of both kinases (Fig. 4D). Modulation of the phosphorylation state of ERK1 and ERK2 by the different treatments was in agreement with results obtained by the measurement of ERK activity.

Fig. 4. A, PACAP-38 stimulated MAP kinase activity. Neurons were incubated with the indicated concentrations of PACAP-38 for 10 min at 37°C. MAPK activity was determined as described in Materials and Methods. Data are expressed as the percentage of MAP kinase activity in LK medium alone. Data are the mean ± SEM of three independent experiments performed in triplicate. B, PACAP-38 and forskolin induced MAPK activation independently of neuronal activity. Neurons were incubated for 1 hr in the presence of 1 µM tetrodotoxin (TTX) and then stimulated with HK medium, 100 nM PACAP-38, or 10 µM forskolin for 10 min. Data are the mean ± SEM of three independent experiments performed in triplicate. *p < 0.005; **p < 0.0005 with Student's t test. Values were compared with the corresponding control (LK alone or LK + TTX). C, PACAP stimulates MAP kinase activity via a PKA- and MEK-dependent mechanism. Neurons were incubated for 1 hr in the presence or absence of different inhibitors (25 µM PD98059; 20 µM H89; 200 µM Rp-cAMP), washed twice, and incubated at 37°C for 10 min with 100 nM PACAP-38 and 10 µM forskolin in the presence or absence of the inhibitors. Data are the mean ± SEM of three independent experiments performed in triplicate. *p < 0.0005 with Student's t test, as compared with (a) LK, (b) PACAP-38 (P38), and (c) forskolin (FK). D, PACAP-38 and forskolin-induced phosphorylation of ERK1 and ERK2. Western blotting with an antibody specific for phosphorylated ERKs was performed as described in Materials and Methods. Western blotting with an antibody against total ERKs is shown to document equal loading.
[View Larger Version of this Image (43K GIF file)]

To test whether PACAP-induced ERK activation was involved in the anti-apoptotic effect of PACAP-38, we measured DNA fragmentation in the presence of PD98059 and Rp-cAMP. Both compounds did not affect the protection induced by high KCl concentrations, excluding toxic or nonspecific effect (Fig. 5). Interestingly, both PD98059 and Rp-cAMP blocked the effect of PACAP-38 on DNA fragmentation (Fig. 5).

Fig. 5. PACAP-38 decreased DNA fragmentation via a PKA- and MEK-dependent mechanism. Neurons were incubated with 25 µM PD98059 or 200 µM Rp-cAMP for 1 hr before the addition of 100 nM PACAP-38 or 2 µg/ml actinomycin D. Cells were washed twice with HK medium and incubated with different drugs and inhibitors for 24 hr. Soluble and nonsoluble DNA were isolated and quantified, as indicated in Materials and Methods. Data are the mean ± SEM of at least three independent experiments. *p < 0.005 with Student's t test, as compared with PACAP-38 alone.
[View Larger Version of this Image (57K GIF file)]

DISCUSSION

It was reported recently that forskolin, a direct activator of adenylyl cyclase, and cholera toxin (CTX), an activator of Gs, protected cerebellar granule cells from cell death (D'Mello et al., 1993; Galli et al., 1995; Yan et al., 1995a). However, no physiological activator of the cAMP pathway displaying the same protective effect was characterized. In the present work, we demonstrated that PACAP-38 was as efficient as forskolin in protecting cerebellar granule cells from KCl deprivation-induced apoptosis. Arimura and coworkers reported a neurotrophic biphasic effect of low PACAP-38 concentrations on gp120-induced apoptosis in hippocampal cultures (Arimura et al., 1994). At concentrations above 1 nM, PACAP-38 was not effective in that system. In contrast, the effect of PACAP-38 on cerebellar granule neurons was monophasic, with a maximum at 100 nM. The effect of PACAP-38 on cerebellar granule neurons is, therefore, likely to involve mechanisms different from those recruited in hippocampal cultures.

Using RT-PCR, we demonstrated the presence of PACAP1-R and PACAP/VIP1-R mRNAs. In addition, the PACAP1-R gene is spliced alternatively and generates different variants with possible insertion of two cassettes named "hip" and "hop" in the third intracellular loop of the receptor (Spengler et al., 1993). RT-PCR experiments revealed the presence of two variants: (1) PACAP1-R s (short), which does not contain any insert, and (2) PACAP1-R hop, a variant with one insertion. These results are in agreement with recently published experiments by D'Agata and coworkers (1996), who showed a major PCR product corresponding to the hop variant at postnatal day 4 (P4) and P8. Interestingly, at P25 the major PCR product corresponded to the short variant, suggesting a possible role for alternative splicing of the PACAP1-R during postnatal development of the cerebellum. We confirmed expression of the PACAP1-R protein by measuring PACAP- and VIP-stimulated cAMP production. Although expression of PACAP/VIP1-R mRNA was demonstrated with RT-PCR, pharmacological characterization indicated that PACAP/VIP1-R protein was expressed at very low level, because VIP did not stimulate cAMP formation potently. The effect of PACAP-38 on cerebellar granule cells was, therefore, mediated mainly by PACAP1-R activation. Basille and coworkers documented the presence of PACAP receptors on cells of the proliferating external granule cell layer (EGL) at P8 (Basille et al., 1993). At that time, granule cells undergo both maximal proliferation and massive DNA fragmentation, indicating that apoptosis occurs in the EGL very soon after neurogenesis, before maximal migration to the internal granule cell layer (IGL), and synaptogenesis with Purkinje cells occurs ~P10. This indicates that factors other than synaptogenesis must regulate the number of granule cells that survive (Wood et al., 1993). The present work suggests that PACAP-38 might be one of these factors.

Most neurotrophic factors concurrently participate in neuronal differentiation and protection from apoptosis. It is, therefore, not surprising that these two processes involve common mechanisms, and studies on neuronal differentiation help to elucidate mechanisms underlying protection from apoptosis. PACAP-38 was shown to display neurotrophic properties in several systems, namely PC12 (Deutsch and Sun, 1992), sympathetic neurons (Pincus et al., 1990; DiCicco-Bloom and Deutsch, 1992), and chick embryo and hippocampal cultures (Arimura et al., 1994). In PC12 cells, it was demonstrated that MAP kinase activation is necessary and sufficient for differentiation (Cowley et al., 1994) and that blockade of the MAP kinase pathway by PD98059, a specific MEK inhibitor (Alessi et al., 1995; Dudley et al., 1995), prevented differentiation of PC12 cells by NGF (Pang et al., 1995). Interestingly, it also was shown that cAMP-induced differentiation was accompanied by activation of the MAP kinase pathway (Frödin et al., 1994; Young et al., 1994) and that PACAP-38 stimulates ERK1 activity (Frödin et al., 1994). These results suggested a possible mechanism for PACAP-38 action on cerebellar granule cells. Conversely, Edwards et al. (1991) demonstrated that cAMP protected sympathetic neurons from NGF withdrawal-induced apoptosis without activating ERK (Virdee and Tolkovsky, 1995). In cerebellar granule cells we demonstrated that PACAP-38 stimulated ERK1 and ERK2 activity and phosphorylation. Interestingly, Rp-cAMP and H89, two inhibitors of PKA, totally blocked ERK stimulation by PACAP-38, indicating that activation of PKA is necessary for PACAP-induced ERK activation (Fig. 4C). In addition, we showed that blockade of the cAMP pathway with Rp-cAMP or of the MAP kinase pathway with PD98059 abrogated the antiapoptotic effect of PACAP-38. Conclusively, protection of cerebellar granule cells by PACAP-38 likely involves the same mechanism as the one suggested in PC12 cells for cAMP-induced differentiation, namely activation of PKA, which stimulates MEK activity, resulting in activation of ERK. The precise pathway linking PKA activation to stimulation of MEK activity remains elusive at present (Frödin et al., 1994; Young et al., 1994).

Interestingly, we also demonstrated that activation of the MAP kinase pathway is not the exclusive way to protect cerebellar granule neurons from KCl deprivation-induced cell death. For instance IGF-1 or high KCl concentration protected neurons (Fig. 1) but weakly stimulated ERK activity (Fig. 4B) (data not shown). Furthermore, the protective effect of KCl was not affected by PD98059 (Fig. 5). This suggests that other pathways, which work independently of ERK activation, possibly are involved in protection from apoptosis. Xia and coworkers (1995) recently proposed that NGF withdrawal-induced apoptosis of PC12 cells requires concurrent activation of the stress kinases [C-Jun N-terminal protein kinase (JNK) and p38] and inhibition of ERK kinases. Hence, either stimulation of ERK activity or inhibition of the JNK/p38 pathway could result in the same protection from apoptosis.

In the present work, we investigated the effect of activation of adenylyl cyclase and ERKs by PACAP-38 during cerebellar granule cells apoptosis and demonstrated that it is necessary to the antiapoptotic effect of PACAP-38. PACAP1-R also stimulates phospholipase C at high PACAP-38 concentrations. Further work will be necessary to evaluate the relevance of possible crosstalk between AC and PLC pathways. Finally, generation of a mouse strain deficient for PACAP1-R gene will be a helpful tool to assess the effect of PACAP-38 on cerebellum development in vivo.

FOOTNOTES

Received Aug. 12, 1996; revised Sept. 30, 1996; accepted Oct. 8, 1996.

  

M.V. was supported by Centre National de la Recherche Scientifique, La Fondation pour la Recherche Médicale (FRM). This work was supported by Grant ACC-SV4/9504087 from the Ministère de l'Education Nationale et de la Recherche, La Ligue pour la Recherche contre le Cancer, and Boehringer-Ingelheim. We gratefully acknowledge the help of Dr. Mireille Lafon for cerebellar granule cell culture and the generous gift of PD98059 by Dr. Alan R. Saltiel (Parke-Davis Pharmaceutical Research).

Correspondence should be addressed to Dr. Laurent Journot, Centre National de la Recherche Scientifique, UPR 9023, CCIPE, 141 Rue de la Cardonille, F-34094 Montpellier Cedex 05, France.


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