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Volume 17, Number 20, Issue of October 15, 1997 pp. 8024-8037
Copyright ©1997 Society for Neuroscience

Regulators of G-Protein Signaling (RGS) Proteins: Region-Specific Expression of Nine Subtypes in Rat Brain

Stephen J. Gold1, 2, Yan G. Ni1, 2, Henrik G. Dohlman3, and Eric J. Nestler1, 2, 3

1 Laboratory of Molecular Psychiatry and Departments of 2 Psychiatry and 3 Pharmacology, Yale University School of Medicine, New Haven, Connecticut 06508

ABSTRACT
INTRODUCTION
MATERIALS AND METHODS
RESULTS
DISCUSSION
FOOTNOTES
REFERENCES

ABSTRACT

The recently discovered regulators of G-protein signaling (RGS) proteins potently modulate the functioning of heterotrimeric G-proteins by stimulating the GTPase activity of G-protein alpha  subunits. The mRNAs for numerous subtypes of putative RGS proteins have been identified in mammalian tissues, but little is known about their expression in brain. We performed a systematic survey of the localization of mRNAs encoding nine of these RGSs, RGS3-RGS11, in brain by in situ hybridization. Striking region-specific patterns of expression were observed. Five subtypes, RGS4, RGS7, RGS8, RGS9, and RGS10 mRNAs, are densely expressed in brain, whereas the other subtypes (RGS3, RGS5, RGS6, and RGS11) are expressed at lower density and in more restricted regions. RGS4 mRNA is notable for its dense expression in neocortex, piriform cortex, caudoputamen, and ventrobasal thalamus. RGS8 mRNA is highly expressed in the cerebellar Purkinje cell layer as well as in several midbrain nuclei. RGS9 mRNA is remarkable for its nearly exclusive enrichment in striatal regions. RGS10 mRNA is densely expressed in dentate gyrus granule cells, superficial layers of neocortex, and dorsal raphe. To assess whether the expression of RGS mRNAs can be regulated, we examined the effect of an acute seizure on levels of RGS7, RGS8, and RGS10 mRNAs in hippocampus. Of the three subtypes, changes in RGS10 levels were most pronounced, decreasing by ~40% in a time-dependent manner in response to a single seizure. These results, which document highly specific patterns of RGS mRNA expression in brain and their ability to be regulated in a dynamic manner, support the view that RGS proteins may play an important role in determining the intensity and specificity of signaling pathways in brain as well as their adaptations to synaptic activity.

Key words: seizure; gene expression; striatum; neocortex; GTPase-activating proteins; Sst2; GAIP

INTRODUCTION

The heterotrimeric G-proteins play a critical role in brain signal transduction by coupling extracellular receptors to intracellular signaling pathways. G-proteins are composed of single alpha , beta , and gamma  subunits. The alpha  subunits are guanine nucleotide-binding proteins that are regulated through cycles of GTP and GDP binding. In the inactive state, the alpha  subunits are bound to GDP. They are activated by G-protein-coupled receptors (bound to ligand), which trigger the dissociation of GDP and the subsequent binding of GTP, which activates the alpha  subunit. The active state is terminated when the intrinsic GTPase activity contained within the alpha  subunit hydrolyzes GTP to GDP (for review, see Neer, 1995).

Recently, a newly discovered class of protein, termed regulators of G-protein signaling (RGS) proteins, has been shown to modulate the functioning of G-proteins by activating the intrinsic GTPase activity of the alpha  subunits (for review, see Dohlman and Thorner, 1997; Koelle, 1997; Neer, 1997). RGS proteins thereby reduce the duration of the activated GTP-bound state of the alpha subunit, which inhibits G-protein function. RGS proteins were first discovered in Saccharomyces cerevisiae, in which mutational analysis revealed a protein, Sst2, with novel activity in inhibiting G-protein function (Chan and Otte, 1982; Dohlman et al., 1995). In the last two years, full or partial sequences of the mRNAs for numerous putative RGS proteins have been identified in diverse species (De Vries et al., 1995; Bruch and Medler, 1996; Chen et al., 1996, 1997; Druey et al., 1996; Koelle and Horvitz, 1996; Faurobert and Hurley, 1997). Eighteen RGS proteins have been identified to date in mammalian tissues; all are highly homologous within a so-called RGS domain. RGS proteins have recently been shown to function as GTPase-activating proteins for the Gi family of alpha  subunit, including various isoforms of Galpha i, Galpha o, and transducin (Galpha t) (Berman et al., 1996a,b; Hunt et al., 1996; Watson et al., 1996; Hepler et al., 1997; Wieland et al., 1997). Some of the proteins also exhibit activity toward Galpha q but no detectable activity toward Galpha s or Galpha 12.

It is striking that the number of known RGS proteins exceeds the number of their target G-protein alpha  subunits. This raises the question of how the specificity of RGS-dependent signaling is achieved. One possibility is that specificity results, at least in part, from different patterns of RGS expression. Indeed, Northern analysis revealed different tissue distributions of a small number of RGS proteins (De Vries et al., 1995; Chen et al., 1996, 1997; Druey et al., 1996; Koelle and Horvitz, 1996) with at least nine of the subtypes being present in brain (Koelle and Horvitz, 1996). We show here striking regional specificity of the expression of these RGS subtypes. We also show that the expression of several subtypes, RGS7, RGS8, and RGS10, is altered by acute seizure, which demonstrates the ability of the RGS proteins to be dynamically regulated in the nervous system. Together, these findings provide new leads to understand in much greater detail the mechanisms of brain signaling pathways and how the required intensity and specificity of signaling may be achieved.

MATERIALS AND METHODS

Animal treatments. Adult male Sprague Dawley rats (250-300 gm; Camm Research Institute, Wayne, NJ) were used for (1) histochemical localization (n = 3), (2) relative regional abundance analysis by Northern blot (n = 2), or (3) studies of electroconvulsive seizure (ECS; n = 20). ECS was administered as described previously (Winston et al., 1990). Briefly, electroconvulsive current (40 mA, 0.3 sec) was administered via bilateral ear clips, and rats were killed 8 hr (n = 5), 24 hr (n = 5), or 7 d (n = 5) later by perfusion; control rats (n = 5) were killed 24 hr after ear clipping. After lethal sodium pentobarbital injections, rats were perfused transcardially with 50 ml of saline in 0.1 M sodium phosphate buffer (PB) followed by 400 ml of 4% paraformaldehyde in 0.1 M PB (PPB). Brains were removed from the skull, post-fixed in PPB for 24 hr, cryoprotected in 20% sucrose in PPB for 24 hr, and then sectioned into cold PPB with a freezing microtome, at 30 µm in the coronal plane, from the nucleus accumbens through the midcerebellum (1 in 12 series, for localization studies) or from the fornix to temporal hippocampus (one in six series, for ECS studies). The olfactory bulb was cut parasaggitally (one in six series). Alternate tissue sections were then hybridized to the nine RGS cRNAs for localization studies or to RGS7, RGS8, or RGS10 cRNAs for the ECS studies.

In situ hybridization. Pretreatment and hybridization were that of Gall et al. (1995). Briefly, sections were permeabilized with proteinase K (1 µg/ml, 37°C, 30 min), treated with acetic anhydride containing triethanolamine (0.1 M), washed in 2× SSC (1× SSC = 0.15 M NaCl and 0.015 M sodium citrate, pH 7.0), and then hybridized free-floating for 24-36 hr at 60°C in buffer containing formamide (50%), polyvinyl pyrrolidone (0.7%), Ficoll (0.7%), bovine serum albumin (7 mg/ml), denatured salmon sperm DNA (0.33 mg/ml), yeast tRNA (0.15 mg/ml), dithiothreitol (40 µM), and cRNA probe at 1 × 107 cpm/ml. Sections were then rinsed in 4× SSC (twice, 60°C), treated with RNase A (20 µg/ml, 30 min at 45°C), washed in descending concentrations of SSC to a stringency of 0.1× SSC at 37°C, mounted onto gelatin-coated microscope slides, and air-dried. Tissue sections were exposed to beta -max Hyperfilm (Amersham, Arlington Heights, IL) for 4-7 d. After film autoradiography, tissue sections were defatted in Americlear clearing reagent (VWR/Baxter, South Plainfield, NJ), dipped in NTB2 emulsion (Eastman Kodak, Rochester, NY), exposed for 2-5 weeks at 4°C, developed with D19 (Kodak), fixed (Regular Fix, Kodak), counterstained with cresyl violet, and coverslipped with Permount.

Riboprobes. Antisense riboprobes were transcribed with T3 RNA polymerase (Stratagene, La Jolla, CA) in the presence of 35S-UTP (in situ hybridization) or 32P-CTP (Northern blot) (Dupont NEN, Boston, MA) and for in situ hybridization, phenol-chloroform-extracted and precipitated twice on dry ice with 0.3 M sodium acetate/ethanol. Riboprobes for Northern analysis were purified with Nuc Trap minicolumns (Stratagene). RGS templates were generated by HindIII (antisense) or BamHI (sense) digestion of pMK167, pMK152, pMK184, pMK186, pMK164, pMK153, pMK162, pMK190, and pMK175 plasmids generously provided by Michael Koelle (Yale University) (Koelle and Horvitz, 1996). All nine constructs contained ~240 bases inserted at the modified EcoRV site of Bluescript SK (Stratagene). Each 240 base insert was composed of a 200 base core sequence (available via GenBank, accession numbers U32434, U32327, U32435, U32436, U32328, U32432, U32433, U32437, and U32438 for RGS3-RGS11, respectively) flanked by ~20 base 5' and 3' PCR primer sequences, which are available on request. The antisense riboprobe sequences were aligned (MacVector, Kodak) and determined to share <= 73% sequence identity. Furthermore, all had similar GTP/CTP and UTP contents. The cyclophilin riboprobe was transcribed with T7 RNA polymerase (Boehringer Mannheim, Indianapolis, IN) from a HindIII digest of pGM-cyclo in which the coding region of rat cyclophilin was inserted into the BamHI site of PGEM-3Z (Promega, Madison, WI). For in situ hybridization, the labeling specificity of the riboprobes was established using sense riboprobes of the respective RGS subtypes. No specific labeling was observed for any of the sense cRNAs.

Northern blot hybridization. Tissue samples were obtained by gross dissection in chilled phosphate buffer containing 0.32 M sucrose, rapidly frozen on dry ice, and stored at -80°C. Samples were homogenized with a Brinkmann homogenizer in 4 M guanidium thiocyanate and 25 mM sodium acetate buffer containing 0.5% 2-mercaptoethanol, pelleted by centrifugation through a 5.7 M cesium chloride gradient at 150,000 × g at 20°C for 18 hr, resuspended in 0.3 M sodium acetate, pH 5.2, and ethanol-precipitated. After the determination of RNA concentration by spectrophotometry, 10 µg of RNA was electrophoresed along with RNA standards (0.24-9.5 kb; Life Technologies, Gaithersburg, MD) through a formaldehyde/1.2% agarose gel containing ethidium bromide, transferred to nitrocellulose membranes (supported nitrocellulose-1, Life Technologies) by capillary blotting, and UV cross-linked to the membrane (Stratalinker, Stratagene). Northern blots were hybridized as described previously (Alvaro et al., 1996) for 18 hr at 65°C in a roller tube oven in buffer containing 20 mM Tris-HCl, pH 7.5, 0.1% sodium pyrophosphate, 0.1% SDS, 0.2% polyvinyl pyrrolidone, 0.2% Ficoll, 5 mM EDTA, 10% dextran sulfate, 4× SSC, 50% deionized formamide, 100 µg/ml denatured salmon sperm DNA and 2 × 106 cpm/ml (RGS) or 2 × 105 cpm/ml (cyclophilin) 32P-labeled riboprobes. Blots were washed to a stringency of 0.1× SSC/0.1% SDS at 65°C, exposed to Hyperfilm MP (Amersham) for 3 d (RGS) or 1 d (cyclophilin), and developed with an X-OMAT film processor (Kodak). Blots were first probed with RGS cRNAs and 1 month later with cyclophilin cRNA.

Densitometry. Labeling densities of RGS7, RGS8, and RGS10 cRNA were determined by densitometry of autoradiographic film using National Institutes of Health Image analysis software, a Macintosh IIfx computer, and Northern Light illumination (Imaging Research, St. Catherine's, Ontario, Canada). Briefly, polynomial calibration curves were fitted to relative optical density values measured from known 14C-labeled radioactive standards (Amersham), which were coexposed to the film with tissue sections and related nanocuries per gram of tissue to relative optical density. Mean regional labeling densities for the dentate gyrus granule cell layer and parietal cortex were taken from at least two brain sections per animal (both hemispheres) and are expressed as nanocuries per gram of tissue. With modifications, abbreviations are those of Paxinos and Watson (1986).

RESULTS

Overview

The labeling patterns produced by the nine RGS cRNAs were highly specific for each RGS subtype. The specificity of hybridization was achieved by the use of high stringency conditions and was verified by hybridization of sense cRNAs for each transcript. General features of subtype cRNA labeling patterns are described in this section and in Table 1. A more detailed analysis of the regional and cellular hybridization patterns will be described in the following section and is summarized in Table 2. Finally, regulation of the expression of RGS7, RGS8, and RGS10 mRNAs by acute ECS is presented.

Table 1. Expression highlights for RGS subtype mRNAs

RGS subtype Regions and cell types where most enriched
RGS3 Principal thalamic relay nuclei and white matter
RGS4 Cortex, thalamus, and striatum, but noticeably low in hippocampus
RGS5 Paraventricular and supraoptic nuclei of hypothalamus, basomedial amygdala, and glia
RGS6 Olfactory bulb, scattered striatal cells, medial habenula, reticular thalamic, subthalamic, and pontine nuclei
RGS7 Widespread, relatively high in cerebellar granule cells
RGS8 Widespread, extremely dense in cerebellar Purkinje cells; relatively dense in basal ganglia-related brainstem nuclei
RGS9 Extremely dense in caudoputamen, nucleus accumbens, and olfactory tubercle; relatively dense in medial hypothalamus
RGS10 Extremely dense in dentate gyrus granule cells; relatively dense in superficial layers of neocortex and dorsal raphe
RGS11 Subfornical organ and locus coeruleus

Table 2. Regional labeling densities for RGS cRNA probes

RGS3 RGS4 RGS5 RGS6 RGS7 RGS8 RGS9 RGS10 RGS11
Telencephalon
  Neocortex
    Layer I 0 0 + 0 0 0 0 + 0
    Layer II 0 +++++ + + +++ +++ 0 +++++ 0
    Layer III 0 +++++ + + +++ 0 0 +++++ 0
    Layer IV 0 + + + + 0 0 + 0
    Layer V 0 +++++ + + + 0 0 + 0
    Layer VI 0 ++ + + + + + + 0
  Piriform cortex + +++++ + + +++++ + + +++ 0
  Nucleus accumbens 0 +++ + 0 + ++++ +++++ + 0
  Caudoputamen 0 +++ + 0 + +++ +++++ ++ 0
  Olfactory tubercle 0 +++ + 0 + ++++ +++++ + 0
  Lateral septum 0 0 + + + 0 0 ++ 0
  Medial septum 0 +++ + 0 + +++ 0 + 0
  Hippocampus
    Stratum pyramidale 0 + + 0 +++ +++ 0 + +
    Stratum granulosum 0 + + 0 +++ + + +++++ +
    Molecular layers 0 0 + 0 0 + 0 + 0
  Amygdala
    bst 0 +++++ 0 0 + + + 0 0
    Central amygdala 0 +++++ + 0 + + + + 0
    Lateral amygdala 0 +++++ + 0 +++ + 0 + 0
    Basolateral amygdala 0 ++++ +++++ 0 +++ + 0 + 0
    Medial amygdala 0 ++ + 0 +++ + ++ + 0
Diencephalon
  Medial habenula 0 + + +++++ + +++++ + 0 0
  Lateral habenula 0 0 + 0 + +++ 0 + 0
  Thalamus
    Principal relay nuc ++++ +++++ + +++a +++ ++++ 0 0 0
    Midline/intralaminar nuc +++ +++++ + 0 +++ +++++ 0 + 0
    Reticular nuc 0 0 + ++++ + 0 0 0 0
  Subthalamic nuc 0 +++ + +++++ ++++ ++++ 0 + 0
  Hypothalamus
    Paraventricular nuc 0 +++++ +++++ 0 ++ 0 ++ 0 0
    Supraoptic nuc 0 +++++ +++++ 0 +++ + +++ 0 0
    Suprachiasmatic nuc 0 ++ + 0 + ++ +++ +++ 0
    Ventromedial nuc 0 + + 0 +++ + +++ 0 0
    Arcuate nuc 0 + + 0 ++ + ++++ 0 0
    Medial mammillary nuc ++ +++++ + ++++ +++++ +++++ 0 + 0
    Lateral mammillary nuc 0 +++++ + 0 ++ 0 0 ++ 0
Brainstem
  Substantia nigra (pc) + + + ++ ++ +++++ 0 0 0
  Ventral tegmental area + + + ++ ++ +++++ 0 0 0
  Central gray 0 +++ + 0 +++ +++++ 0 ++ 0
  Superior colliculus 0 + + + +++ +++++ 0 +++ 0
  Inferior colliculus + + + 0 +++ +++ 0 +++++ 0
  Interpeduncular nuc + + + +++ +++ ++++ 0 + 0
  Pontine nuc 0 ++++ +++ +++++ +++ +++++ 0 +++ +
  Dorsal raphe 0 0 + + ++ ++++ + +++++ +
  Locus coeruleus +++ +++++ + +++ +++++ +++++ + 0 +++
  Cerebellum
    Purkinje cell layer + + + + 0 +++++ 0 + 0
    Granule cell layer 0 0 0 0 ++++ + 0 + 0
Regional labeling densities were determined by visual inspection of autoradiographic films. Labeling densities are as follows: very dense, +++++; dense, ++++; moderately dense, +++; light, ++; faint, +; and not detectable, 0. Abbreviations: bst, Bed nucleus of the stria terminalis; nuc, nucleus; pc, pars compacta. a Labeling restricted to subset of anterior thalamic nuclei.

Of the nine RGS subtypes surveyed, five of the cRNAs densely labeled brain. These included RGS4, RGS7, RGS8, RGS9, and RGS10 cRNAs. Although specific labeling in brain was also observed for the four other subtypes, labeling tended to be at a lower density and in more restricted sets of brain regions. Labeling by RGS3 cRNA was restricted to dorsal thalamus and white matter. RGS4 cRNA most densely labeled several layers of neocortex, piriform cortex, caudoputamen, and ventrobasal thalamus. RGS5 cRNA densely labeled only three neuronal populations, the paraventricular and supraoptic hypothalamic nuclei and parts of the basolateral amygdaloid complex. In addition, diffuse labeling across all tissue sections and dense labeling in glia-enriched layers of hippocampus suggested glial expression of RGS5 mRNA. RGS6 cRNA most densely labeled the olfactory bulb granule cell layer, scattered cells in the caudoputamen, the reticular thalamic nucleus, the medial habenula, the subthalamic nucleus, and several other brainstem nuclei. Labeling by RGS7 cRNA was widespread. RGS8 cRNA labeling was extremely dense in the cerebellar Purkinje cell layer, with less dense yet still marked labeling occurring in numerous other regions. Labeling by RGS9 cRNA was remarkable for its nearly exclusive enrichment in striatum. RGS10 cRNA labeling was by far most enriched in the dentate gyrus granule cell layer. Finally, RGS11 cRNA labeling was restricted to the subfornical organ and the caudal locus coeruleus.

Detailed analysis

The cRNAs used for this analysis are identical in length, contain similar levels of GTP and CTP, and have comparable levels of 35S-UTP incorporation. Additionally, and unless noted, photomicrographs of cRNA labeling for the nine RGSs were taken from film autoradiograms of identical exposure times. Thus, the panels represent reasonable approximations of relative mRNA densities for the RGS subtypes. All estimations of relative labeling densities between RGS cRNAs were determined by visual inspection of the film autoradiograms. Importantly, for each cRNA, the hybridization patterns were virtually indistinguishable between rats (n = 3) and independent hybridizations (>= 2).

Telencephalon In olfactory bulb (Fig. 1), five of the RGS subtype mRNAs showed moderate to high levels of expression. Dense RGS4 cRNA labeling was restricted to the mitral, tufted cell layer. RGS6 and RGS10 cRNAs labeled the granule cell layer moderately densely. RGS7 and RGS8 mRNAs were expressed in several different layers of bulb. Both of these subtype cRNAs had moderate labeling densities in the glomerular cell layer. In the mitral, tufted cell layer, however, RGS7 cRNA labeling was dense, whereas RGS8 cRNA labeling was only moderately dense.
Fig. 1. Bright-field photomicrographs of film autoradiograms of in situ hybridization to the nine RGS cRNAs through parasaggital sections of olfactory bulb. In this figure and in Figures 2, 3, 4, 5, 6, 7, 8, 9, 10, exposure times were identical for all nine probes. mt, Mitral, tufted cell layer; g, granule cell layer; on, olfactory nerve layer; opposing arrows, glomerular cell layer. Scale bar, 1.7 mm.
[View Larger Version of this Image (157K GIF file)]

The principal features of neocortical labeling can be appreciated in Figures 2, 3, 4. Subtype cRNA labeling in neocortex was by far most dense for RGS4. Indeed it appeared that RGS4 cRNA labeling in cortex was denser than in any other brain region examined. Less yet considerable hybridization was present for RGS7 and RGS10 cRNAs. The laminar profile of subtype cRNA labeling can be seen in Figures 2, 3, 4, 5 and is detailed more fully in Table 2. With the exception of RGS10 cRNA, all subtype cRNAs had similar laminar expression patterns between cortical areas. For RGS10, however, cRNA labeling was relatively less dense in superficial layers of frontal and cingulate/retrosplenial cortices. Interestingly, in emulsion autoradiograms of RGS8 cRNA hybridization, densely labeled cells were diffusely distributed across neocortical layers, with no apparent relation to laminar boundaries (Fig. 5B).

Fig. 2. Bright-field photomicrographs of film autoradiograms illustrating the labeling patterns of RGS cRNAs through rostral striatum and nucleus accumbens. Note the striking enrichment of (1) RGS4 cRNA labeling in neocortex (Neo) and (2) RGS9 cRNA labeling in caudoputamen (cp), accumbens (acb), and olfactory tubercle (ot). Also note the lower RGS10 cRNA labeling densities in frontal (fr) and cingulate (cg) cortices relative to parietal cortex (par). cc, Corpus callosum; pc, piriform cortex. Scale bar, 1.1 mm.
[View Larger Version of this Image (164K GIF file)]

Fig. 3. Bright-field photomicrographs of film autoradiograms showing the hybridization patterns of RGS3-RGS11 cRNAs. Note the overall paucity of labeling in lateral septum (ls) and the relatively dense labeling of RGS4 and RGS8 cRNAs in medial septum (ms). cp, Caudoputamen; ot, olfactory tubercle. Scale bar, 1.4 mm.   
[View Larger Version of this Image (187K GIF file)]

Fig. 4. Bright-field photomicrographs of film autoradiograms of RGS cRNA labeling patterns in coronal planes of section through septal hippocampus (hip). bla, Basolateral amygdaloid nucleus; cc, corpus callosum; cp, caudoputamen; la, lateral amygdaloid nucleus; mea, medial amygdaloid nucleus; mhb, medial habenula; th, thalamus; pv, paraventricular thalamic nucleus; slm, stratum lacunosum moleculare; rt, reticular thalamic nucleus; vmh, ventromedial hypothalamic nucleus; opposing arrows, hippocampal stratum pyramidale; asterisk, ventrobasal thalamus. Scale bar, 1.8 mm.
[View Larger Version of this Image (176K GIF file)]

Fig. 5. Dark-field photomicrographs of emulsion autoradiograms showing cRNA labeling patterns across laminae of neocortex (A, B), dentate gyrus (C) and cerebellar cortex (D). Note that exposure times varied between subtype cRNAs and are not intended to depict relative labeling densities between RGS cRNAs. Representative labeling patterns for RGS4 cRNA (A) and RGS8 cRNA (B) are illustrated in cross-sections of parietal neocortex and temporal neocortex, respectively. Layers are indicated by numbers with the exception of layer 1 in A (unlabeled zone at top). Note the scattered cells (arrows) densely labeled by RGS8 cRNA. In C, fibrillar RGS5 cRNA labeling excludes both the dentate gyrus granule cell layer (opposing arrows) and CA3 stratum pyramidale (sp) but is enriched in the dentate gyrus molecular layer (m) and hilus (hr). In D, the extremely dense RGS8 cRNA labeling of the cerebellar Purkinje cell layer (P) is apparent. g, Cerebellar granule cell layer. Scale bar: A, B, 260 µm; C, D, 290 µm.
[View Larger Version of this Image (115K GIF file)]

In the rostral caudoputamen and nucleus accumbens, the most striking feature was the extremely dense RGS9 cRNA labeling throughout the entire rostral and caudal extent of the structures (Figs. 2, 3, 4). Other subtypes with considerable cRNA labeling densities in these basal ganglia structures were RGS4, RGS8, and RGS10. In addition to the dense expression of these four subtype mRNAs, emulsion autoradiograms revealed that scattered cells throughout the caudoputamen were densely labeled by RGS6 cRNA (Fig. 6A). In lateral septum, the only RGS cRNAs showing appreciable labeling were those for RGS6 and RGS10. Even then, they labeled at only a moderate density (Figs. 3, 6A). In medial septum and the vertical limb of the diagonal band of Broca, RGS4 and RGS8 cRNAs labeled most densely.

Fig. 6. Dark-field photomicrographs of emulsion autoradiograms illustrating subtle features of RGS6, RGS4, and RGS5 cRNA labeling. Note that subtype exposure times varied and hence do not reflect relative labeling densities between RGS cRNAs. A, Dense RGS6 cRNA labeling of scattered cells in caudoputamen (cp) and of the anterior commissural nucleus (ac). Less dense labeling occurred in neocortex (Neo), lateral septum (ls), and piriform cortex (pc). B, C, Dense labeling by RGS4 and RGS5 cRNAs in paraventricular hypothalamic nucleus (pav). Note also the relatively low levels and complete absence of RGS4 cRNA labeling in hippocampus (hip) and anterior thalamus (a), respectively. Finally, note the fibrillar appearance of RGS5 cRNA labeling throughout the tissue section and dense labeling of the vasculature (arrowheads). cea, Central amygdaloid nucleus; gp, globus pallidus; bla, basolateral amygdaloid nucleus. Scale bar, 1.2 mm.
[View Larger Version of this Image (97K GIF file)]

In hippocampus, overall labeling was most dense for RGS7, RGS8, and RGS10 cRNAs. In the dentate gyrus granule cell layer, labeling by RGS10 cRNA was extremely dense, whereas that for RGS7 cRNA was substantially less dense (Fig. 4). In the pyramidal cell layers, RGS7 and RGS8 cRNA labeling predominated. Nevertheless, in temporal hippocampus, RGS4 cRNA labeled the pyramidal cell layer at a significant density (data not shown). In addition to labeling within the principal neuronal layers, cells labeled by RGS8 and RGS10 cRNAs were scattered across the hippocampal molecular layers. Finally, diffuse RGS5 cRNA labeling was most enriched in CA1 stratum lacunosum moleculare (Figs. 4, 5C).

Across the amygdaloid complex, patterns of labeling were remarkably differentiated, and for RGS4 cRNA in particular, differed across subregions of specific nuclei. In the nucleus of the lateral olfactory tract, RGS4 and RGS8 cRNA labeling was most dense (data not shown). In the bed nucleus of the stria terminalis and the central amygdaloid nucleus, subtype cRNA labeling was most dense for RGS4 with less dense cRNA labeling for RGS7, RGS8, and RGS9 (Fig. 6B). The lateral and basolateral amygdaloid nuclei were also densely labeled by RGS4 cRNA (subregions; Figs. 4, 6B). Minor contributions to the hybridization signal came from the RGS7 cRNA. In the basomedial and basoventral subnuclei of the basolateral nucleus, however, RGS5 cRNA labeling was clearly predominant (Fig. 4). In the medial amygdaloid nuclei, total cRNA labeling densities were relatively lower, with the predominant subtypes being RGS4, RGS7, and RGS9 (Fig. 4).

Diencephalon In the majority of dorsal thalamus, labeling was dominated by RGS3, RGS4, RGS7, and RGS8 cRNAs (Fig. 4). At least two notable exceptions to this pattern were observed. In the anterior thalamic nuclei there was a conspicuous absence of labeling by RGS4 cRNA and a notable presence of labeling by RGS6 cRNA (Fig. 6B). The balance of labeling in the ventrobasal complex also deviated from the prototypical thalamic pattern, with particularly dense labeling by RGS4 cRNA (Fig. 4).

Outside of dorsal thalamus, the balance of cRNA labeling differed in at least five ways. First, hybridization to RGS3 cRNA did not extend beyond dorsal thalamus. Second, within the lateral parafascicular thalamic nucleus, RGS8 was by far the most densely labeling subtype cRNA (Fig. 7). Third, in the reticular thalamic nucleus, subtype cRNA labeling was dominated by RGS6 (Fig. 4). Fourth, in medial habenula, labeling densities were highest for RGS8 cRNA and slightly less for RGS6 cRNA (Figs. 4, 7). Finally, in the subthalamic nucleus, labeling was most dense for RGS6 cRNA with just less dense labeling by RGS7 and RGS8 cRNAs (Fig. 7). As in many other regions, dense RGS4 cRNA labeling in the subthalamic nucleus was restricted to a subregion.

Fig. 7. Bright-field photomicrographs of film autoradiograms of in situ hybridization to the nine RGS cRNAs in coronal sections through caudal diencephalon. arc, Arcuate nucleus; dlg, dorsolateral geniculate nucleus; mhb, medial habenula; pf, lateral parafascicular thalamic nucleus; po, posterior thalamic nucleus; sth, subthalamic nucleus; vlg, ventrolateral geniculate nucleus; zi, zona incerta; asterisk, ventrobasal thalamus; Scale bar, 1.4 mm.
[View Larger Version of this Image (160K GIF file)]

Although most hypothalamic nuclei appeared to be labeled by one or more of the RGS cRNAs, a complete description of labeling patterns within hypothalamus is beyond the scope of this paper. Nevertheless, hypothalamic labeling highlights will be described briefly. Throughout hypothalamus, RGS7 and RGS8 cRNA labeling were most ubiquitous. In addition, RGS9 cRNA tended to label numerous medial hypothalamic nuclei at a moderate to high density.

The supraoptic and paraventricular nuclei had similar cRNA labeling patterns; RGS4 and RGS5 cRNAs labeled densely (Fig. 6B,C), whereas RGS7 and RGS9 cRNAs were minor contributors. The suprachiasmatic nucleus exhibited a different pattern; RGS9 and RGS10 cRNAs labeled at the highest density, with slightly lower labeling densities for RGS4 and RGS8 cRNAs. Yet a third pattern emerged for the ventromedial and arcuate nuclei; moderately dense labeling by RGS7 and RGS9 cRNAs was predominant, with lighter labeling by RGS4 and RGS8 cRNAs (Figs. 4, 7).

Mesencephalon and caudal brainstem As with hypothalamus, descriptions of RGS cRNA labeling will be limited to several major mesencephalic and metencephalic structures. As can be seen in Figure 8, labeling in the substantia nigra pars compacta and ventral tegmental area was dominated by RGS8 cRNA. In central gray, labeling for RGS7 and RGS8 cRNAs was predominant. (Figs. 8, 9) Moderate labeling by RGS4 cRNA was present in the dorsal aspect of the central gray. The inferior and superior colliculi shared similar cRNA labeling patterns such that in both regions RGS8 and RGS10 cRNAs labeled most densely, with slightly lower levels for RGS7 cRNA (Figs. 9, 10). Finally, in the pontine nuclei, total cRNA labeling was very dense. The RGS6 cRNA labeled most densely. Slightly less dense labeling occurred for RGS5, RGS7, RGS8, and RGS10 cRNAs (Figs. 9, 10). Similar to numerous other brain regions, RGS4 cRNA labeling densities were heterogeneous across the pontine nuclei.
Fig. 8. Bright-field photomicrographs of film autoradiograms illustrating RGS cRNA labeling in coronal sections through the mesodiencephalic border. 3n, Oculomtor nucleus; apt, anterior pretectal nuclei; cg, central gray; dk, nucleus darkewisch; mg, medial geniculate nucleus; sc, superior colliculus; sn, substantia nigra pars compacta; v, ventral tegmental area. Scale bar, 1.2 mm.
[View Larger Version of this Image (148K GIF file)]

Fig. 9. Bright-field photomicrographs of film autoradiograms showing in situ hybridization of RGS cRNAs in coronal sections through superior colliculus (sc) and pontine nuclei (pn). cg, Central gray; dr, dorsal raphe; pb, parabigeminal nucleus. Scale bar, 1.0 mm.   
[View Larger Version of this Image (121K GIF file)]

Fig. 10. Bright-field photomicrographs of film autradiograms of in situ hybridization of RGS cRNAs in coronal sections through the inferior colliculus (ic) and pontine nuclei. dtg, Dorsal tegmental nucleus; lc, locus coeruleus; rtg, reticulotegmental nucleus; ll, nucleus of the lateral lemniscus. Scale bar, 1.0 mm.   
[View Larger Version of this Image (125K GIF file)]

In cerebellar cortex, the most striking feature was the extremely dense labeling of the Purkinje cell layer by RGS8 cRNA (Figs. 5D, 11). A more modest signal was observed there for RGS10 cRNA. Almost as striking as cerebellar RGS8 cRNA labeling was the moderately dense RGS7 cRNA labeling of the granule cell layer.

Fig. 11. Bright-field photomicrographs of film autoradiograms of RGS cRNA labeling in coronal planes through cerebellum at the level of the caudal locus coeruleus. lc, Locus coeruleus; sp5o, spinal trigeminal nucleus oralis; vc, ventral cochlear nucleus; 7, facial nucleus. Scale bar, 1.7 mm.
[View Larger Version of this Image (157K GIF file)]

Labeling in the dorsal raphe was dominated by RGS10 cRNA (Fig. 9). In locus coeruleus, labeling was dominated by RGS4 and RGS7 cRNAs, with a lesser yet considerable presence of RGS3, RGS8, and RGS11 cRNA labeling (Fig. 11).

Northern blot analysis To substantiate the findings from in situ hybridization, four of the nine RGS cRNAs were used to screen Northern blots of neocortical, hippocampal, striatal, and cerebellar RNA. Relative hybridization densities across the four brain regions were in good agreement with that found using in situ hybridization and served as an additional confirmation of the hybridization specificity of these cRNAs. Thus, the hybridization signal was greatest in (1) neocortex for RGS4, (2) cerebellum for RGS8, (3) striatum for RGS9, and (4) hippocampus and striatum for RGS10. Furthermore, RGS4, RGS8, RGS9, and RGS10 cRNAs hybridized to principal bands of unique sizes corresponding to 3.4, 6.6, 2.6, and 1 kb transcript lengths, respectively (Fig. 12). Minor hybridization bands were also observed for RGS9 and RGS8.
Fig. 12. Northern Blot hybridization of 32P-labeled cRNAs for RGS4, RGS8, RGS9, RGS10, and cyclophilin (cyclo) to whole mRNA (10 µg/lane) from neocortex (Ctx), hippocampus (Hip), caudoputamen (CP), and cerebellum (Cb) of rat. All four RGS probes hybridize to clear principal bands, with hybridization most enriched in cortex, cerebellum, and caudoputamen for RGS4, RGS8, and RGS9, respectively. For RGS10, hybridization is similarly dense in both hippocampus and caudoputamen. The RGS8 and RGS9 cRNAs also hybridize to several minor bands. Kilobase markers indicate significant bands of hybridization for each RGS cRNA.   
[View Larger Version of this Image (48K GIF file)]

Regulation of RGS10 and other subtypes by seizure

In several eukaryotic systems, RGS activity is regulated at the level of expression (for review, see Dohlman and Thorner, 1997). To begin to examine the degree to which RGSs in brain might be similarly regulated, we used the ECS paradigm. Acute ECS induces dramatic regulation of numerous brain mRNAs and proteins in discrete brain regions and has been used as a model of activity-dependent changes in gene expression (see Hope et al., 1994; Nibuya et al., 1996). We focused on one of the most profoundly affected brain regions, the dentate gyrus granule cell layer (gcl), where RGS10 is by far the most abundant subtype mRNA (see Fig. 4). In addition, the gcl is a remarkably homogenous neuronal population. Given the cellular homogeneity and the predominance of RGS10 mRNA, changes in RGS10 levels could have profound effects on G-protein signaling in these neurons. As can be seen in Figure 13, a single ECS induced time-dependent decreases in RGS10 mRNA in both the gcl and superficial pyramidal layers of parietal neocortex. In gcl, decreases were apparent by 8 hr after ECS and maximal at 24 hr, falling to almost 60% of control levels, but had largely returned to normal by 7 d after ECS. Changes in neocortex followed a similar time course but were more modest. In addition to RGS10, the response to acute ECS of RGS7 and RGS8 mRNA, two transcripts present in the gcl at more modest concentrations, were also assessed. Changes in these transcripts were more transient, with significant changes occurring only 8 hr after ECS [RGS7 increased by 36% (p < 0.01, Dunnett's multiple comparison test), and RGS8 decreased by 14% (p < 0.01, Dunnett's multiple comparison test)].
Fig. 13. RGS10 mRNA is downregulated after acute ECS stimulation. At 24 hr after acute ECS (B), RGS10 cRNA labeling is decreased in parietal neocortex (open arrows) and dentate gyrus granule cell layer (closed arrows) relative to control levels (A). The time course of changes (C) shows that a marked decrease occurs between 8 and 24 hr after ECS, and that labeling densities have returned close to control levels by 7 d after ECS. *p < 0.05; **p < 0.01 (ANOVA, Dunnett's test). Scale bar, 1.5 mm.   
[View Larger Version of this Image (53K GIF file)]

DISCUSSION

The major finding of the present study is the distinct regional specificity of the mRNAs for nine subtypes of RGS proteins, which had previously been shown by PCR or Northern blot analysis to be present in brain (Druey et al., 1996; Koelle and Horvitz, 1996). Some mRNAs are broadly distributed, whereas others are highly localized and relatively restricted to a small number of brain regions. The patterns of expression of the RGS mRNAs are consistent with neuronal localization, although there were indications that at least some of the RGS mRNAs were expressed in glia.

One of the most dramatic patterns of expression was observed for RGS9 mRNA, which was highly enriched in striatal regions, including caudoputamen, nucleus accumbens, and olfactory tubercle. In this respect, the pattern of expression of RGS9 mRNA resembles that for many other striatal-enriched proteins, including several subtypes of dopamine receptors (Meador-Woodruff, 1994), Galpha olf (Herve et al., 1993), adenylyl cyclase type V (Glatt and Snyder, 1993), and DARPP-32 and other striatal-enriched phosphoproteins (Hemmings et al., 1989; Brene et al., 1994). Given this localization for RGS9 mRNA, and the likelihood that this protein is at least somewhat specific for Gi- and Go-coupled receptors, one possibility is that RGS9 specifically modulates signaling via the D2-like dopamine receptors. Although RGS9 mRNA is highly enriched in striatal regions, it is not the only RGS subtype mRNA found at appreciable levels in these regions. This is analogous to some of the other striatal-enriched signaling proteins, for example, adenylyl cyclase type V and Galpha olf. Although these subtypes are enriched in striatum, several other forms of adenylyl cyclase and G-protein alpha  subunits are expressed at high levels in these regions.

Patterns of expression of other RGS subtypes are also noteworthy. RGS4 mRNA is perhaps the most widely distributed and highly expressed RGS subtype mRNA in brain. It is present at high levels in cerebral cortex, striatum, and thalamus as well as in several brainstem nuclei. Within cortex, RGS4 mRNA shows a striking layer-specific distribution. RGS8 mRNA was interesting for its expression in several brainstem nuclei involved in basal ganglia function. For example, it was expressed at high levels in substantia nigra, ventral tegmental area, and lateral parafascicular and subthalamic nuclei. RGS10 mRNA was striking for its intense enrichment in the dentate gyrus granule cell layer, although it was also found in several other brain regions at lower yet substantial levels, including dorsal raphe and superficial layers of neocortex. RGS3 mRNA was largely restricted to thalamus, although it was not by any means the most abundant RGS mRNA in this brain region. It must be emphasized that all of these expression patterns are based on levels of mRNA for the various RGS proteins. A major focus of future research will be to define the patterns of expression of the proteins themselves. This will require the development of subtype-specific antibodies, which are not available now.

Given the proposed role of RGS proteins as GTPase-activating proteins for G-protein alpha  subunits, one major question is: By what mechanisms are RGS proteins themselves regulated in the brain? In yeast, the best established mechanism of regulation is at the level of RGS expression (Dohlman and Thorner, 1997). A similar phenomenon has been observed in lymphocytes, in which several types of stimuli lead to enhanced expression of various RGS subtypes (Murphy and Norton, 1990; Hong et al., 1993; Siderovski et al., 1994). The physiological role served by this regulation remains unknown, because these observations were made before the functional activity of the RGS proteins was established. Nevertheless, increased levels of RGS proteins were shown to inhibit G-protein signaling in transfected cells (Druey et al., 1996; Neill et al., 1997). This was expected because increased RGS levels would lead to increased GTPase activity of the associated G-protein alpha  subunits and thereby to decreases in the duration of the activated G-protein signal. Such regulation could exert profound effects on cell functioning.

As a first attempt to assess possible regulation of RGS proteins in brain, we examined RGS7, RGS8, and RGS10 mRNA levels in hippocampus after an acute ECS. We chose these three subtypes, because they are highly expressed in hippocampus, which is known to undergo intense epileptiform activity during this treatment. We found that levels of RGS10 mRNA were reduced by almost half in the dentate gyrus, with maximal effects observed between 8 and 24 hr after the ECS. Levels recovered within 7 d. Similar, albeit less robust, regulation of RGS10 mRNA was observed in superficial layers of neocortex. A corresponding decrease in RGS10 protein levels would be expected to result in the facilitation of Gi- and Go-mediated receptor signaling, which could, in turn, represent an adaptive response to the intense synaptic stimulation by ECS of a host of neurotransmitters known to act through Gi and Go in hippocampus (e.g., norepinephrine, serotonin, and several neuropeptides). The observed decrease in RGS10 expression is particularly noteworthy, because it is the first demonstration that levels of an RGS protein can be downregulated. The responses of RGS7 and RGS8 mRNA levels to ECS were more transient, with a significant increase in RGS7 and a small decrease in RGS8 seen 8 hr after treatment.

Although these findings are clearly just the initial characterization of the regulation of RGS proteins in brain, they do nevertheless raise the interesting possibility that regulation of RGS proteins may be widespread and may contribute in important ways to adaptive responses in brain signal transduction pathways. If this proves true, then RGS proteins could provide novel mechanisms for desensitization and sensitization of G-protein-coupled receptor function. These studies also highlight the unique power of model organisms, such as S. cerevisiae and Caenorhabditis elegans, in revealing new families of proteins, the homologs of which subserve essential signaling functions in mammalian brain.

Results of the present study provide fundamental information concerning the localization of a newly discovered class of signaling protein in the brain. The highly region-specific patterns of RGS mRNA expression suggest that certain subtypes may be associated with specific types of receptors or other signaling proteins by virtue of their coexpression in the same tissue. In addition, it will be important in future investigations to determine whether the various RGS proteins also exhibit distinct regulatory and functional properties and whether they show distinct subcellular localizations. This information promises to expand our knowledge of the vast repertoire of molecular mechanisms governing the intensity and cell type specificity of signaling in the nervous system. In addition, because the expression of RGS proteins is, in many cases, considerably more restricted than that of most G-proteins or G-protein-coupled receptors, RGS proteins represent novel and potentially exciting targets for the development of new psychotropic medications.

FOOTNOTES

Received May 29, 1997; revised Aug. 1, 1997; accepted Aug. 5, 1997.

  

This work was supported by National Institutes of Health Grants DA08227 to E.J.N. and GM55316 to H.G.D. and by the Abraham Ribicoff Research Facilities of the Connecticut Mental Health Center, State of Connecticut Department of Mental Health and Addiction Services. We thank Dr. Michael Koelle for his generous gift of RGS cDNAs and for helpful discussions and Dr. Philip Iredale for striatal RNA.

Correspondence should be addressed to Eric J. Nestler, Department of Psychiatry, Yale University, 34 Park Street, New Haven, CT 06508. 


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