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Previous Article | Next Article
Volume 17, Number 7, Issue of April 1, 1997 pp. 2295-2313
Copyright ©1997 Society for NeuroscienceDistribution of Brain-Derived Neurotrophic Factor (BDNF) Protein and mRNA in the Normal Adult Rat CNS: Evidence for Anterograde Axonal Transport
James M. Conner1, Julie C. Lauterborn2, Qiao Yan3, Christine M. Gall2, and Silvio Varon1 1 Department of Biology, 0506, University of California, San Diego, La Jolla, California 92093, 2 Department of Anatomy and Neurobiology, University of California, Irvine, California 92697, and 3 Amgen Center, Thousand Oaks, California 91320
ABSTRACT
A sensitive immunohistochemical technique was used, along with highly specific affinity-purified antibodies to brain-derived neurotrophic factor (BDNF), to generate a detailed mapping of BDNF immunoreactivity (BDNF-ir) throughout the adult rat CNS. A parallel analysis of sites of BDNF synthesis was performed with in situ hybridization techniques using a cRNA probe to the exon encoding mature rat BDNF protein. These combined data revealed (1) groups of cell bodies containing diffuse BDNF-ir throughout the CNS that were strongly correlated with fields of cells containing BDNF mRNA; (2) varying degrees of BDNF-ir outside of cell bodies, in what appeared to be fibers and/or terminals; and (3) many regions containing extremely heavy BDNF-immunoreactive fiber/terminal labeling that lacked BDNF mRNA (e.g., medial habenula, central nucleus of the amygdala, bed nucleus of stria terminalis, lateral septum, and spinal cord). The latter observation suggested that in these regions BDNF was derived from anterograde axonal transport by afferent systems. In the two cases in which this hypothesis was tested by the elimination of select afferents, BDNF immunostaining was completely eliminated. These data, along with the observation that BDNF-ir was rarely found within dendrites or fibers en passage, suggest that BDNF protein produced in adult CNS neurons is polarized primarily along axonal processes and is preferentially stored in terminals within the innervation target.
Key words: neurotrophin; immunohistochemistry; in situ hybridization; neurotrophic factor; BDNF; anterograde axonal transport
INTRODUCTION
The neurotrophins are a related family of growth factors presently consisting of nerve growth factor (NGF), brain-derived neurotrophic factor (BDNF), neurotrophin-3 (NT-3), NT-4/5, and NT-6 (Berkemeir et al., 1991; Narhi et al., 1993
; Gotz et al., 1994
To date, many investigators have reported on the localization of mRNAs for the various neurotrophins, although few of these studies have provided a detailed mapping throughout the CNS. NGF expression has been localized primarily to the hippocampal formation, olfactory bulb, and cortex
all target regions of basal forebrain cholinergic neurons (Korsching et al., 1985
The cellular localization of neurotrophin proteins in the CNS has proceeded much more slowly, probably reflecting the technical limitations inherent in the immunohistochemical approach. Recently, we have generated antibodies and developed fixation and immunohistochemical protocols permitting the visualization of NGF protein in the normal adult rat brain (Conner et al., 1992
In the present investigation, we have used the sensitive immunohistochemical protocol developed for NGF, along with a well characterized preparation of affinity-purified polyclonal antibodies to BDNF (Yan et al., 1997
MATERIALS AND METHODS
BDNF antibodies and cDNA probes. Affinity-purified rabbit polyclonal antibodies to BDNF used in this investigation were generated and characterized as described previously (Conner et al., 1996
BDNF immunohistochemistry. Adult male and female Sprague Dawley rats (Simonsen, Gilroy, CA, and Harlan, San Diego, CA) were used (n = 5 for normal distribution; n = 14 for analysis of lesion effects). All animals were perfused under deep anesthesia with ~50 ml PBS followed with ~250 ml 2% paraformaldehyde + 0.2% parabenzoquinone in 0.07 M phosphate buffer, pH 7.2. Brains were removed, post-fixed for 2 hr in the same fixative, and cryoprotected overnight in 30% sucrose in 0.1 M phosphate buffer (all solutions at 4°C). Coronal sections (40 µm) were cut on a sliding microtome and stored in Millonig's buffer until they were processed for BDNF-ir as has been described (Conner et al., 1996
In situ hybridization for BDNF mRNA. Sprague Dawley rats (Simonsen) (n = 5) were anesthetized by intraperitoneal injection with sodium pentobarbital and killed by perfusion with 50 ml 0.9% saline followed by 350 ml 4% paraformaldehyde in 0.1 M phosphate buffer (PPB). Brains were removed from the cranium and post-fixed in PPB for 24 hr at 4°, cryoprotected in 20% sucrose in PPB for 24-48 hr, and then sectioned on a freezing microtome at a thickness of 25 µm in the coronal plane. Tissue sections were processed free-floating as described previously (Lauterborn et al., 1991 -max) and emulsion (Kodak NTB2) autoradiography, with exposure times of 3-4 d at room temperature and 4-6 weeks at 4°C, respectively. After autoradiographic development, emulsion-coated slides were counterstained with cresyl violet, coverslipped with Permount, and analyzed by bright- and dark-field microscopy.
Lesions. Unilateral (n = 4) or bilateral (n = 4) aspirative lesions of the septofimbrial and triangular septal nuclei were accomplished by removing a 3 × 3 mm area of skull on either side of Bregma and immediately lateral to the sagittal sinus. After the dura was opened, successive aspiration of part of the parietal and cingulate cortices, the supracallosal stria, and the corpus callosum exposed the fimbria and supracommissural septal nuclei. Aspiration of the fimbria and supracommissural septal nuclei was verified visually, and the resulting gap was filled with Gelfoam soaked in sterile PBS. Unilateral (n = 4) or bilateral (n = 2) lesions of the pontine parabrachial nuclei were accomplished by passing a 1 mA current for 20 sec through a tungsten electrode placed 11.5 mm caudal to bregma, 1.9 mm lateral from the midline, and 7.6 mm down from the skull surface (with the electrode carrier angled 20° anterior). For both lesion paradigms, animals survived for 2 weeks after surgery and were then perfused under deep anesthesia. The precise location of all lesions and the extent of the damage was assessed histologically.
RESULTS
Immunostaining for BDNF revealed specific labeling throughout the full extent of the CNS that was confined to select populations of cells or fibers/terminals in well defined regions (for an overview, see Fig. 1). The anatomical nomenclature used throughout this description was taken from Paxinos and Watson (1988). Results presented in the tables are a summary of data obtained by the microscopic observation (made independently by two different investigators) of histological slides from many animals, and they may differ slightly from what appears in any single photograph. BDNF cellular staining always appeared as a diffuse reaction product distributed throughout the perikaryal cytoplasm and occasionally extended into the most proximal processes, but it did not occupy the cell nucleus (Fig. 2A). The assessment of fiber/terminal staining was made on the basis of high-magnification examination of the tissue and was characterized as (1) coarse, with distinctly defined individual fibers (e.g., spinal cord and spinal trigeminal nucleus) (Fig. 2B), (2) diffuse and finely punctate with individual fibers not distinguishable (e.g., hypothalamus) (Fig. 2C), or (3) heavy punctate, in what appeared to be noded processes that formed pericellular baskets around unstained neuronal cell bodies (e.g., bed nucleus of the stria terminalis, lateral septum, central nucleus of amygdala) (Fig. 2D). Throughout the CNS, the cellular localization of BDNF immunostaining and mRNA was restricted to cells possessing a neuronal morphology. In all cases, the specificity of the immunostaining was confirmed by omitting the primary antibody or through the use of preadsorption controls (Fig. 3).
Fig. 1. Distribution of BDNF-ir in the adult rat brain. Both plates show at low magnification a rostrocaudal series of sections (left to right) that were obtained from two female rats immunostained using affinity-purified antibodies specific for BDNF (bright-field illumination). BDNF immunostaining in male rats (not shown) was indistinguishable from that seen in females. Scale bar, 3 mm.
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Fig. 2. Characteristic patterns of BDNF immunostaining in the rat CNS. Panels show examples of BDNF immunostaining in basolateral amygdaloid nucleus (A), spinal cord (B), hypothalamus (C), and lateral septum (D) that are representative of the quality of immunolabeling seen throughout the CNS (bright-field illumination). As shown in A, perikaryal labeling was characterized by a diffuse reaction product distributed throughout the cell cytoplasm but excluding the cell nucleus. B-D, Various types of fiber/terminal staining. As seen in spinal cord lamina 1 (B, small arrow) and laminae 3 (B, open arrows), numerous individual fibers were immunolabeled; immunoreactive fibers were also concentrated in lamina 2 (B, large arrow). Other fields contained punctate immunostaining that appeared either diffuse or clustered around unlabeled cell bodies (C, arrows), or well defined, with discrete pericellular baskets around unlabeled cells (D, arrow). Scale bars (shown in B): A, B, 100 µm; (shown in D) C, D, 50 µm.
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Fig. 3. Specificity of BDNF immunostaining. A-C, Tissue sections processed for immunohistochemistry with either the BDNF antibody omitted (A), the BDNF antibody solution preadsorbed against purified recombinant human BDNF (B), or the BDNF antibody solution preadsorbed against mouse
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In the following paragraphs the distributions of both BDNF-ir and BDNF cRNA hybridization throughout the CNS will be described. Although tissue from separate sets of rats was processed for the two techniques, these data will be presented together for the sake of comparison within a field.
The distribution of BDNF protein and mRNA in the olfactory bulb, cortex, and hippocampal formation is summarized in Table 1. In the main olfactory bulb, a few periglomerular cells were lightly labeled for both BDNF mRNA and protein. In the mitral cell layer, a few cells were lightly labeled for BDNF-ir but not for mRNA, and in the granule cell layer, a faint haze of autoradiographic grains, indicative of low mRNA content, was distributed over the entire region, but no BDNF-ir was detected. In contrast, granule cells in the accessory olfactory bulb were lightly immunostained but not labeled by the cRNA. In the anterior olfactory nucleus (Fig. 4A-C), all subregions contained numerous cells that were well labeled for BDNF mRNA and moderately labeled for BDNF-ir. Moderate BDNF immunostaining and hybridization were detected in nearly all cells within the rostral portion of the tenia tecta but were nearly absent from more caudal sections. In contrast, many cells containing BDNF-ir and BDNF mRNA were distributed within the intermediate lateral septal nucleus adjacent to the tenia tecta at this level (Fig. 4D-F). Throughout the rostrocaudal extent of the claustrum and endopiriform nucleus, many cells were heavily labeled for BDNF mRNA and moderately heavily labeled for BDNF-ir (Fig. 4G,H).
Table 1. BDNF in the olfactory bulb, cortex, and hippocampal formation
BDNF-ir fibers BDNF-ir cells BDNF mRNA Mitral cell layer 2/l 0 Internal granule layer 0 4/l Glomerular layer (periglomerular) 1/l 2/l External plexiform/tufted cells 0 0 Granule cell layer of AOB ± 2/l-m 0 Anterior olfactory nucleus + 3/m 3-4/h Orbital cortex 3/l 2-3/m Insular cortex (granular + agranular) 3/l-m 3/m-h Frontal cortex 3/m 3/m Cingulate cortex 3/l-m 3-4/h Piriform cortex 3/m 3-4/h Parietal cortex 3/l 2-3/m Perirhinal cortex 3/l-m 3/m-h Retrosplenial cortex 3/l 3/m Occipital cortex 2-3/l 2-3/m Temporal cortex 3/l 2-3/m Entorhinal cortex 3/m-h 3/m-h Claustrum ± 3-4/h 3-4/h Endopiriform nucleus (dorsal/ventral) 2-3/m 3/h Tenia tecta (rostral part) 3/m 4/h Tenia tecta (caudal part) 0 4/l Indusium griseum 2/l-m 1/m CA1-stratum oriens ++ 1/l 0 CA1-stratum pyramidale 2/l-m 3-4/m CA1-stratum radiatum ++ 0 0 CA1-stratum lacunosum moleculare ++ 0 0 CA2-stratum oriens ++ 1/m 0 CA2-stratum pyramidale 3/l-m 4/h CA2-stratum lucidum +++++ 0 0 CA3-stratum oriens ++ 1/m 0 CA3-stratum pyramidale 4/m-h 4/h CA3-stratum lucidum +++++ 0 0 Dentate gyrus-polymorph layer/hilus +++++ ** 1-2/h Dentate gyrus-granule cell layer 4/l-m 4/m Dentate gyrus-inner molecular layer ++++ 0-1/m 0 Dentate gyrus-middle molecular layer + 0 0 Dentate gyrus-outer molecular layer 0 0 Subiculum ± 2/m 3-4/m Presubiculum 0 2/l Parasubiculum 0 2/l Fibers: , none; +, very light staining; ++, light staining; +++, moderate staining; ++++, heavy staining; +++++, extremely heavy staining. Cells: 0, no cells observed; 1, occasional cells; 2, few scattered cells; 3, moderate number of cells; 4, densely packed cells; ** , could not be determined. l, Lightly stained; m, moderately stained; h, heavily stained.
Fig. 4. BDNF-ir and mRNA in forebrain regions. A-J, Sections through the anterior olfactory nucleus (A-C), caudal portion of the tenia tecta (D-F), claustrum and piriform cortex (G, H), and septal region (I, J) processed for immunohistochemistry (C, F, H, J; bright field) and in situ hybridization (B, E, G, I; dark field). Photomicrographs in A and D are of Nissl-stained tissue. Patterns of BDNF-ir and cRNA labeling were very similar in the anterior olfactory nucleus and rostral tenia tecta (TT) (A-C) and, more caudally, in the claustrum (Cl), endopiriform nucleus (DEn), and piriform cortex (Pir) (G, H). In the caudal portion of the tenia tecta, very light hybridization but no immunostaining were observed (D-F); notably heavy hybridization and immunostaining were present in the adjacent intermediate lateral septum (LSI) (D-F). In septal regions, cRNA-labeled cells were seen only in the medial septum (MS), whereas intense BDNF-ir was localized mostly to fibers/terminals in the lateral septum (I, J). Scale bars (shown in C): A-C, 500 µm; (shown in F): D-F, 200 µm; (shown in H): G, H, 500 µm; (shown in J): I, J, 500 µm. ac, Anterior commissure; AI, agranular insular cortex; AOD, anterior olfactory nucleus, dorsal part; AOP, anterior olfactory nucleus, posterior part; E/OV, ependymal layer/olfactory ventricle; gcc, genu of the corpus callosum; HDB, horizontal limb of the diagonal band; LSD, lateral septum, dorsal part; LSV, lateral septum, ventral part; lv, lateral ventricle; VDB, vertical limb of the diagonal band.
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In cortex, BDNF-ir and cRNA-labeled cells were distributed throughout all rostrocaudal levels with both hybridization and immunostaining densities varying markedly across cortical regions (Fig. 5, Table 1). In nearly all cortical regions, immuno- and hybridization-labeling were observed in layers II, III, V, and VI, with only a few lightly labeled cells in layer IV. The most robust neocortical labeling was often seen deep in layer VI adjacent to the corpus callosum, especially in parietal cortex (Fig. 5A-C). By comparison, fewer labeled cells were present in layer VI of temporal cortex (Fig. 5D-F). Within a given field, the laminar patterns of hybridization and immunolabeling were generally the same, as shown for entorhinal cortex in Figure 5G-I. Layer I of all cortical regions was completely lacking BDNF immunostaining or cRNA hybridization. 
Fig. 5. Comparison of the distributions of BDNF-ir and mRNA in cortical fields. Sections through parietal (A-C), temporal (D-F), and entorhinal (G-I) cortices were processed for in situ hybridization to localize BDNF mRNA (B, E, H; dark field) or immunohistochemistry to localize BDNF-ir (C, F, I; bright field). Sections through similar planes were Nissl-stained (A, D, G; bright field). In parietal cortex (A-C), the BDNF cRNA heavily labeled neurons in layer VI, moderately in layers II/III, and lightly in layer V; BDNF-ir was very heavy in deep layer VI, lighter in layers II, III, V, and superficial layer VI, and almost undetectable in layer IV. In temporal cortex (D-F), BDNF mRNA was moderately labeled in layers II/III and V/VI, with lighter labeling in layer IV. BDNF-ir in temporal cortex was heaviest in layers II/III, moderate in layers V/VI, and light in layer IV. In entorhinal cortex (G-I), the patterns of BDNF cRNA hybridization and immunoreactivity were similar. Scale bars (shown in A): A-C, 500 µm; (shown in D): D-F, 500 µm; (shown in G): G-I, 1 mm. cc, Corpus callosum.
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In the hippocampus, BDNF immunostaining was detected in both cell bodies and fibers/terminals (Fig. 6). In the pyramidal cell layer, the intensity of cRNA hybridization and immunostaining and the number of cells labeled varied across subfields (Fig. 6B,C). In CA1, a few scattered cells were cRNA-labeled with a moderate density of autoradiographic grains, and a few cells had detectable immunoreactivity. In CA2, most cells had moderate to heavy cRNA labeling and light to moderate levels of immunostaining. In CA3, both cRNA labeling and BDNF-ir were relatively dense in nearly all cells (although it was often difficult to distinguish immunolabeled CA3 pyramidal cells because of the intense BDNF immunostaining within the mossy fiber zone). Only an occasional immunolabeled cell was detected in stratum oriens of CA1-CA3, and no mRNA-positive cells were observed in this layer. Immunoreactive fibers were distributed throughout regions CA1-CA3. As illustrated for region CA1 in Figure 6D, the density of fiber staining varied across the hippocampal laminae, with greater immunostaining in strata oriens and radiatum and less BDNF-ir in strata lacunosum moleculare and pyramidale. In the dentate gyrus, nearly all cells in stratum granulosum were mRNA-positive and BDNF-immunoreactive (Fig. 6B,C,E). In the dentate molecular layer, a distinctive laminar pattern of BDNF-ir was seen (Fig. 6E). Immunostaining was moderate in the inner molecular layer, light in the middle molecular layer, and not detectable in the outer molecular layer. In the hilus, some cells were heavily labeled by the cRNA, and a few immunoreactive perikarya were visible; however, the dense immunohistochemical labeling of fibers in the deeper hilus prevented identification of immunoreactive cells in this field (Fig. 6C). The field of fiber labeling in the deep hilus was the most dense and well stained in the entire CNS and corresponded to the location of the mossy fiber axons of the granule cells. 
Fig. 6. Distribution of BDNF-ir and mRNA in hippocampus. Low-magnification (A-C) and high-magnification (D, E) photomicrographs of sections through hippocampus that were either Nissl-stained (A, bright field) or processed for in situ hybridization (B, dark field) or immunohistochemistry (C-E, bright field). In hippocampus, the BDNF cRNA densely labeled pyramidal cells in CA3, CA2, and the hilus, and less densely the CA1 pyramidal cells and the dentate gyrus granule cells (GrDG) (B). BDNF immunostaining densely labeled processes within the mossy fiber system (C). At higher magnification, one could see that in region CA1 BDNF immunostaining was diffuse in strata oriens (Or) and radiatum (Rad) and weaker in stratum lacunosum moleculare (LMol) (D). Scattered neurons in the CA1 pyramidal cell layer (Py) were immunolabeled (D). In the dentate gyrus (DG) molecular layer, a trilaminar pattern of BDNF-ir was seen, with moderately dense labeling in the inner molecular layer (iml), light labeling in the middle molecular layer (mml), and no detectable staining in the outer molecular layer (oml). Scale bars (shown in C): A-C, 500 µm; (shown in D): D, E, 100 µm and 75 µm, respectively. LHb, Lateral habenular nucleus.
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The distributions of BDNF-ir and mRNA within the basal forebrain and basal ganglia are summarized in Table 2. In the basal forebrain, many densely immunostained and BDNF mRNA-positive cells were distributed in the rostral part of the intermediate lateral septum (Fig. 4D-F). In other basal forebrain regions, such as medial septal nucleus (Fig. 4I), vertical limb of the diagonal band (Fig. 4I), magnocellular preoptic nucleus, medial preoptic area, and septohypothalamic nucleus, there were a few BDNF mRNA-positive cells. In these regions, only an occasional BDNF-immunoreactive cell was seen (Fig. 4J). In addition, many lightly immunostained cells were observed in the triangular septal nucleus and the septofimbrial nucleus (not shown). Immunoreactive fibers also were detected in many basal forebrain regions. In the dorsal and ventral portions of the lateral septum, fibers were well labeled and often appeared to form pericellular baskets around unstained perikarya (Figs. 2D, 4J). In contrast, the medial septum was relatively devoid of fiber staining (Fig. 4J). Moderate BDNF-immunoreactive fiber labeling also was observed in the septohypothalamic nucleus and the subfornical organ. In the basal ganglia, no BDNF mRNA and little BDNF-ir was detected in either cells or fibers. Single, well labeled BDNF-immunoreactive cells with a neuronal morphology were only occasionally observed in the dorsal most aspect of the striatum and in the adjacent corpus callosum.
Table 2. BDNF in the basal forebrain, amygdala, and basal ganglia
BDNF-ir fibers BDNF-ir cells BDNF mRNA Lateral septal nucleus, intermediate ++ 3 /m-h 3/m-h Lateral septal nucleus, dorsal/ventral +++ 0 0 Medial septal nucleus + 1/m 2-3/l-m Diagonal band nucleus, horizontal limb +/++ 1/m 0 Diagonal band nucleus, vertical limb +/++ 1/m 2/l-m Nucleus basalis of Meynert ++ 0 0 Ventral pallidum 0 0 Islands of Calleja 0 0 Lateral preoptic nucleus ++ 1/l 0 Magnocellular preoptic nucleus 1/l 2/l Medial preoptic area ++ 1/l 2-3/m Medial preoptic nucleus +++ 2/h 2-3/m Substantia inominata + 1/l-m 0 Bed nucleus of the stria terminalis, medial/lateral/ventral/intermediate ++/+++ 0 0 Bed nucleus of the stria terminalis, juxtacapsular/lateral dorsal ++++ 0 0 Septohypothalamic nucleus +++ 1/m 2/m Olfactory tubercle + 0 0 Optic chiasm 0 0 Suprachiasmatic nucleus + 0 0 Triangular septal nucleus ± 3/l 0 Septofimbrial nucleus ++ 3/l 3-4/m Subfornical organ ++/+++ 0 0 Corpus callosum 1/m 0 Caudate putamen ± 1/m 0 Globus pallidus 0 0 Accumbens nucleus + 0 0 Nucleus of the lateral olfactory tract, part 1 0 0 Nucleus of the lateral olfactory tract, part 2 2-3/l 3/l Nucleus of the lateral olfactory tract, part 3 2-3/m 2/m-h Central amygdaloid nucleus, lateral ++++ 0 0 Central amygdaloid nuclues, medial ++ 0 0 Basolateral amygdaloid nucleus, anterior 3/h 3-4/h Basolateral amygdaloid nucleus, ventral 2/m 2/m-h Basolateral amygdaloid nucleus, posterior 2/m-h 3/h Medial amygdaloid nucleus, ventral + 1/l-m 3/m-h Cortical amygdaloid nucleus + 2/l-m 3/m Basomedial amygdaloid nucleus + 1/l-m 3/l-m Lateral amygdaloid nucleus 1/l-m 2/m-h Anterior amygdaloid area + 1/m 1/l Amygdalohippocampal area + 2/m 2-3/m-h Amygdalostriatal transition area ++ 0 0 Fibers:
As seen in Table 3, BDNF-ir and mRNA were found in many areas of the thalamus and mesencephalon, with distinctions between regions containing labeled cells and immunoreactive fibers. For example, the parafascicular thalamic nucleus (Fig. 7 G,H) contained numerous mRNA-positive and immunoreactive perikarya but no immunostained fibers. In contrast, in the anteroventral and anteromedial nuclei there was moderate fiber immunostaining, but there was almost no cellular hybridization or immunoreactivity (Fig. 7A,B). Within the dorsal and ventral lateral geniculate nuclei many immunoreactive fibers were present, but immunoreactive or cRNA-labeled cell bodies were not (Fig. 8I,J). In some fields the distributions of hybridization and immunolabeling were similar. For example, in the medial geniculate, the dorsal and ventral portions were devoid of immunolabeling and hybridization, whereas the medial portion of this nucleus and the suprageniculate nucleus contained moderate perikaryal hybridization and immunolabeling (Fig. 7M,N). In the superior colliculus, cRNA hybridization and immunoreactivity also overlapped in layers deep to the superficial gray layer (Fig. 7I,J).
Table 3. BDNF in the thalamus/mesencephalon
BDNF-ir fibers BDNF-ir cells BDNF mRNA Paratenial thalamic nucleus + 0 0 Paraventricular thalamic nucleus ++ 1-2/l-m 3/m Central medial thalamic nucleus ++ 3/l-m 3/l-m Central lateral thalamic nucleus ± 1-2/l 2/l Intermediodorsal thalamic nucleus ++ 3/m 3/l-m Interanterodorsal/interanteromedial thalamic nucleus ++ 0 Light haze Anterodorsal thalamic nucleus ± 3-4/l 3-4/l Anteroventral thalamic nucleus ++/+++ 0 0 Anteromedial thalamic nucleus ++ - Light haze Mediodorsal thalamic nucleus + 1/l-m 0 Laterodorsal thalamic nucleus, dorsomedial + 0 2/l Laterodorsal thalamic nucleus, ventrolateral + 0 0 Ventrolateral/ventromedial thalamic nucleus 0 0 Ventral posterior/posterior 0 0 Reticular thalamic nucleus 0 0 Reuniens thalamic nucleus ++ 0 2/m Rhomboid thalamic nucleus + 2/l-m 2/m Zona incerta +/++ 0 0 Parafascicular thalamic nucleus 4/l 3/m Posterior intralaminar thalamic nucleus ++ 2/m 2/m Lateral posterior thalamic nucleus, mediocaudal ++ 0 0 Lateral geniculate nucleus (dorsal/ventral) ++/+++ 0 0 Medial geniculate nucleus (dorsal/ventral) 0 0 Medial geniculate nucleus, medial + 3/m 3/m Suprageniculate thalamic nucleus + 3/m 3/m Subthalamic nucleus 3-4/l 3/l Peripeduncular nucleus ++ 2/m 2-3/m Medial pretectal area ++ 2/m-h 2-3/m-h Parabigeminal nucleus 3/m 3/l-m Ventral tegmental area +/++ 2/l 3/m-h Substantia nigra, compacta +/++ 2/l 2-3/m-h Substantia nigra, reticular 0 0 Substantia nigra, lateral ++ 2/m-h 2/m-h Nucleus of the posterior commissure ++ 1/m 2/m Retroethmoid nucleus ++ 0 0 Medial accessory occulomoter nucleus + 1/m 1/m Superior colliculus, superficial gray layer + 0 0 Superior colliculus, optic nerve layer 0 3-4/l Superior colliculus, intermediate and deep gray layer + 3/l 0 Precommissural nucleus ++ 0 2-3/l-m Fibers: 
Fig. 7. Localization of BDNF-ir and mRNA in thalamic and brainstem regions. Dark-field and bright-field photomicrographs showing BDNF cRNA hybridization (A, C, E, G, I, K, M, O, Q) and BDNF immunolabeling (B, D, F, H, J, L, N, P, R), respectively, in thalamus (A, B), mammillary region (C, D), locus coeruleus (E, F), parafascicular nucleus (PF) (G, H), superior colliculus (I, J), inferior olivary complex (K, L), medial geniculate nucleus (M, N), parabrachial region (O, P), and the nucleus of the solitary tract (Q, R). In many thalamic (A, B, G, H, M, N) and hypothalamic (C, D) areas, the distributions of BDNF mRNA and perikaryal BDNF-ir overlapped; although in some regions [e.g., anteroventral thalamic nucleus (AV)] there was heavy fiber/terminal BDNF-ir in the absence of hybridization (A, B). In other regions, such as the locus coeruleus (LC) (E, F) and nucleus of the solitary tract (Sol) (Q, R), cRNA-labeled cells could be detected, but it was difficult to identify immunostained cells because of the fiber/terminal immunolabeling. Weak labeling with both the cRNA and antibody also overlapped in most layers of superior colliculus, excluding the superficial gray layer, which did not contain cRNA labeling (I, J). In contrast, heavy hybridization and BDNF-ir were detected throughout the inferior olivary complex (K, L). Moderate to heavy BDNF cRNA labeling was found in the external portion of the lateral parabrachial nucleus (LPBE), whereas heavy fiber/terminal BDNF-ir was present in most subregions of the lateral parabrachial subfield (LPB) (O, P); neither cRNA nor antibody labeled the medial portion of the parabrachial nucleus (MPB) (O, P). Scale bars (shown in B, D, J, N): A-D, I, J, M, N, 500 µm, respectively; (shown in F): E, F, 200 µm; (shown in H, P, R): G, H, O, P-R, 250 µm, respectively. AP, Area postrema; CG, central (periaqueductal) gray; DpG, superior colliculus, deep gray layer; DpMe, deep mesencephalic nucleus; DTg, dorsal tegmental nucleus; fr, fasciculus retroflexus; Gr, gracile nucleus; IAM, interanteromedial thalamic nucleus; InG, superior colliculus, intermediate gray layer; IOA, inferior olive, subnucleus A; IOC, inferior olive, subnucleus C; IOD, inferior olive, dorsal nucleus; LDVL, laterodorsal thalamic nucleus, ventrolateral; LH, lateral hypothalamic area; LM, lateral mammillary nucleus; LRt, lateral reticular nucleus; MG, medial geniculate nucleus; MGM, medial geniculate nucleus, medial portion; MM, medial mammillary nucleus; Op, superior colliculus, optic nerve layer; PIL, posterior intralaminar nucleus; PrC, precommissural nucleus; PP, peripeduncular thalamic nucleus; PT, paratenial thalamic nucleus; PV, paraventricular thalamic nucleus; py, pyramidal tract; scp, superior cerebellar peduncle; SG, suprageniculate nucleus; SubC, subcoeruleus nucleus; SuG, superior colliculus, superficial gray layer; SuM, supramammillary nucleus; VL, ventrolateral thalamic nucleus.
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Fig. 8. Dense fiber/terminal BDNF-ir was present in many regions lacking BDNF mRNA expression. A-L, Dark-field and bright-field photomicrographs of sections processed for in situ hybridization (A, C-E, I, K) and immunohistochemistry (B, F-H, J, L), respectively, through the amygdala (A, B), habenula (C, F), bed nucleus of stria terminalis (D, G), spinal cord (E, H), lateral geniculate nucleus (I, J), and dorsal/ventral tegmental nucleus (K, L). As seen in A and B, in the basolateral (BLA) and lateral amygdala (La), many cells were heavily labeled for both mRNA and BDNF-ir, whereas in the central amygdala many immunolabeled fibers/terminals were present but cRNA hybridization was not. Similarly, in the medial habenular nucleus (MHb) (C, F) subregions of the bed nucleus of stria terminalis (D, G), the dorsal (DLG) and ventral regions (VLG) of the lateral geniculate nucleus (I, J), the intergeniculate leaf (IGL) (I, J), and ventral tegmental nucleus (VTg) (K, L), heavy fiber/terminal BDNF-ir was found without detectable mRNA. In spinal cord (cervical level shown), heavily immunostained fibers were present primarily in lamina 2 (H), which lacked BDNF mRNA (E). Scale bars (shown in B): A, B, 500 µm; (shown in F): C, D, F, G, 500 µm; (shown in H): E, H, 150 µm; (shown in J, L): I-L, 500 µm. ac, Anterior commissure; BSTLD, bed nucleus of stria terminalis, lateral dorsal; BSTLJ, bed nucleus of the stria terminalis, juxtacapsular; BSTM, bed nucleus of stria terminalis, medial; CeL, central amygdaloid nucleus, lateral division; CeM, central amygdaloid nucleus, medial division; CPu, caudate putamen (striatum); DEn, dorsal endopiriform nucleus; DG, dentate gyrus; DTg, dorsal tegmental nucleus; HF, hippocampal formation; ic, internal capsule; LatC, lateral cervical nucleus; LC, locus coeruleus; LHb, lateral habenular nucleus; lv, lateral ventricle; opt, optic tract; Pir, piriform cortex; PV, paraventricular thalamic nucleus; SHy, septohypothalamic nucleus; SubG, subgeniculate nucleus; 4V, 4th ventricle.
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As illustrated in Figure 8A,B and summarized in Table 2, striking patterns of BDNF mRNA and BDNF-ir were seen in the amygdaloid complex. The basolateral, medial, and basomedial amygdaloid nuclei contained many heavily cRNA-labeled and BDNF-immunoreactive cell bodies but very few immunoreactive fibers. In contrast, the central nucleus of the amygdala (especially the lateral subdivision) contained absolutely no BDNF mRNA or BDNF-immunoreactive cells but did contain high densities of immunolabeled fibers, which often formed pericellular baskets around unlabeled perikarya. Within the bed nucleus of the stria terminalis (Fig. 8D,G), heavy pericellular fiber labeling was observed in the lateral dorsal and juxtacapsular regions, whereas other aspects of this nucleus had moderate densities of immunoreactive fibers. BDNF mRNA was not detected in any portion of the bed nucleus of the stria terminalis.
In the hypothalamus, varying degrees of cellular labeling for BDNF-ir and mRNA were observed and a low to moderate level of immunoreactive fiber labeling was present in many areas (Table 4, Fig. 1). A few lightly cRNA-labeled cells were scattered in the lateral and lateroanterior hypothalamic nuclei and extended into the tuber cinereum area. More heavily cRNA-labeled and immunoreactive cells were distributed in the ventromedial hypothalamic nucleus and in the posterior hypothalamic area. Within the paraventricular hypothalamic nucleus, numerous cells of the medial and ventral parvocellular region were well labeled with the cRNA, whereas cells in the lateral magnocellular region were not labeled. Within the mammillary and supramammillary region, many cells were positive for both mRNA and immunoreactivity (Fig. 7C,D). Interestingly, BDNF-immunoreactive fibers but no immunoreactive or cRNA-labeled cell bodies were observed in the median eminence and the infundibular stem. A similar pattern was also seen in the medial habenular nucleus, which contained a very high density of immunoreactive fibers but lacked detectable cellular labeling (immunoreactivity or hybridization) (Fig. 8C,F).
Table 4. BDNF in the hypothalamus and epithalamus
BDNF-ir fibers BDNF-ir cells BDNF mRNA Medial habenular neucleus +++++ 0 0 Lateral habenular nucleus + 1-2/l Light haze Anterior hypothalamic area, anterior ++ 1/l 0 Lateroanterior hypothalamic nucleus ++ 1/l 2-3/l Lateral hypothalamic area ++ 1/m 2/l Anterior hypothalamic area, central ++ 0 0 Paraventricular hypothalamic nucleus (parvocellular) ++/+++ 1/m 3/m Paraventricular hypothalamic nucleus, lateral (magnocellular) + 0 0 Medial tuberal nucleus ++ 1/l-m 2-3/m Tuber cinereum area ++ 1/l-m 2-3/l-m Ventromedial hypothalamic nucleus ++ 2/m-h 3/h Arcuate hypothalamic nucleus +++ 0 0 Median eminance +/++ 0 0 Dorsomedial hypothalamic nucleus, diffuse ++ 1/m 3/l-m Dorsal hypothalamic area ++ 1/m 1/l Perifornical nucleus ++ 0 1/m Premammillary nucleus ++ 0 2-3/m-h Supramammillary nucleus ++ 2/m 3/h Medial mammillary nucleus 4/l-m 3-4/m-h Lateral mammillary nucleus 4/l 3-4/m Interpeduncular nucleus + 0 0 Posterior hypothalamic area ++ 2/l-m 3/m Infundibular stem +/++ 0 0 Fibers:
In the brainstem, many BDNF cRNA-labeled cells were observed, and with a few exceptions the distribution of these cells strongly correlated with the distribution of immunoreactive cell bodies (Table 5). Some notable exceptions were the lateral parabrachial nucleus (Fig. 7O,P), locus coeruleus (Fig. 7E,F), and nucleus of the solitary tract (Fig. 7Q,R), which contained densely cRNA-labeled cells but no detectable immunoreactive perikarya. Fiber immunostaining was present in all three regions, although in the lateral parabrachial nucleus (especially within the external region) and nucleus of the solitary tract, the high density and unique characteristics of the fiber staining may have obscured immunoreactive cell bodies. Conversely, in some brainstem nuclei, such as spinal vestibular nucleus, central nucleus of the inferior colliculus, and gigantocellular reticular nucleus, cell bodies were lightly immunostained but BDNF mRNA was not detected. Very heavy fiber immunolabeling was noted in many brainstem regions, such as the lateral parabrachial nucleus, nucleus of the solitary tract (Fig. 7R), area postrema, inferior olive (Fig. 7L), and ventral tegmental nucleus (Fig. 8L). As seen in Table 5, no cRNA hybridization or immunostaining was detected in the cerebellum.
Table 5. BDNF in the brainstem, cerebellum, and spinal cord
BDNF-ir fibers BDNF-ir cells BDNF mRNA Central gray +++ l/m 3/m Dorsal raphe nucleus ++ 1/l 0 Deep mesencephalic nucleus + 2/m 2/l Pedunculopontine tegmental nucleus 3/m 3/m Pontine nuclei 4/l-m 4/l-m Spinal vestibular nucleus 2/m 0 Ventral tegmental nucleus ++++ 0 0 Cuniform nucleus ++ 1/m 3/m Peritrigeminal zone + 2/m 0 Laterodorsal tegmental nucleus + 0 0 Laterodorsal tegmental nucleus, ventral + 0 0 Dorsal tegmental nucleus, central/pericentral + 0 0 Periolivary nucleus, medioventral/lateroventral +++ 0 0 Inferior colliculus, nucleus brachium + 2/l 1-2/l Inferior colliculus, central nucleus + 2-3/l 0 Inferior colliculus, dorsal cortex + 1-2/l 0 Inferior colliculus, external cortex 3/l-m 2/l Locus coeruleus ++ 0 3/l-m Subcoeruleus nucleus + 2-3/m 2-3/l-m Lateral parabrachial nucleus, internal/dorsal/central +++ 0 3/m Lateral parabrachial nucleus, external ++++ 0 3/h Medial parabrachial nucleus ± 0 0 Principal sensory trigeminal nucleus, ventrolateral 2/m 3/m Nucleus of the solitary tract
ABSTRACT
