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Volume 17, Number 9, Issue of May 1, 1997 pp. 3157-3167
Copyright ©1997 Society for Neuroscience

The 5-HT3 Receptor Is Present in Different Subpopulations of GABAergic Neurons in the Rat Telencephalon

Marisela Morales and Floyd E. Bloom

The Scripps Research Institute, Department of Neuropharmacology, La Jolla, California 92037

ABSTRACT
INTRODUCTION
MATERIALS AND METHODS
RESULTS
DISCUSSION
FOOTNOTES
REFERENCES

ABSTRACT

The type 3 serotonin receptor (5-HT3R) is a ligand-gated ion channel whose presence in the CNS has been established by radioligand binding, in situ hybridization, and immunohistochemical analysis. To analyze further the role of the 5-HT3R in the CNS, we used in situ hybridization and immunocytochemistry to determine that 5-HT3R-expressing neurons are mainly GABA-containing cells in the rat telencephalon. We determined that 5-HT3R/GABA-containing neurons do not exhibit somatostatin immunoreactivity but often contain cholecystokinin (CCK) immunoreactivity. 5-HT3R-expressing cells with CCK immunoreactivity were observed in the neocortex, olfactory cortex, hippocampus, and amygdala. The 5-HT3R/CCK interneurons represent between 35 and 66% of the total population of CCK-containing cells in the neocortex.

Further characterization of the 5-HT3R/GABAergic neurons was based on their calcium-binding protein immunoreactivity and showed that these neurons lack parvalbumin (PV) and represent a subpopulation of calbindin (CB)-containing interneurons that were preferentially present in the CA1-CA3 subfield of the hippocampus. Although some 5-HT3R/GABAergic neurons with calretinin (CR) were found in the neocortex, olfactory cortex, hippocampus, and amygdala, these neurons were more often present in the agranular insular and piriform cortices.

We conclude that the neuronal expression of the 5-HT3R is selective within the GABA neuron population in the rat telencephalon. These 5-HT3R-expressing interneurons might contain CCK, CB, and CR. We suggest that serotonin through the 5-HT3R may regulate GABA and CCK neurotransmission in the telencephalon.

Key words: 5-HT3R; serotonin; GABA; interneurons; calcium-binding proteins; cholecystokinin

INTRODUCTION

The neurotransmitter serotonin [5-hydroxytryptamine (5-HT)] interacts with several receptors (Hoyer et al., 1994), all of which are G-protein-coupled (Hoyer et al., 1994), with the exception of the 5-HT3 receptor (5-HT3R), which is a ligand-gated ion channel (Derkach et al., 1989). Electrophysiological studies on neurons and neuronal cell lines indicate that stimulation of the 5-HT3R causes a rapid depolarization produced by an increased membrane permeability to monovalent cations (Peters and Lambert, 1989). The cloning of a functional subunit of the 5-HT3R (subunit A) from the neuroblastoma cell line NCB-20 has confirmed that the 5-HT3R is a member of the superfamily of ligand-gated ion channels and is structurally related to the nicotinic, GABAA, and NMDA receptors (Manricq et al., 1991).

Radioligand binding studies have detected 5-HT3R binding sites in the CNS of rodents, primates, and humans (Kilpatrick et al., 1987, 1988, 1989; Waeber et al., 1988, 1989, 1990; Barnes et al., 1989a, 1990; Pratt et al., 1990; Gehlert et al., 1991; Jones et al., 1992; Laporte et al., 1992). High-density 5-HT3R binding sites have been found in the hindbrain: the nucleus of the tractus solitarius, area postrema, nucleus of the spinal tract of the trigeminal nerve, and dorsal motor nucleus of the vagus (Waeber et al., 1988, 1989, 1990; Barnes et al., 1990; Pratt et al., 1990; Gehlert et al., 1991; Jones et al., 1992; Laporte et al., 1992). Moderately dense 5-HT3R binding sites have been found consistently in cortex, amygdala, and hippocampus (Kilpatrick et al., 1987, 1988; Waeber et al., 1988; 1989; 1990; Barnes et al., 1989a; Gehlert et al., 1991; Jones et al., 1992; Laporte et al., 1992). In contrast, only a few studies have reported specific binding sites in the nucleus accumbens, striatum, or substantia nigra (Kilpatrick et al., 1987; Waeber et al., 1988; Gehlert et al., 1991; Laporte et al., 1992). The presence of neurons containing 5-HT3R has been confirmed in some of these brain areas by in situ hybridization (Tecott et al., 1993; D. Johnson and S. Heinemann, personal communication) and immunocytochemical analysis (Morales et al., 1996a).

The function of the 5-HT3R in the CNS as well as in the neuronal circuits in which this receptor might participate remains to be established. The use of 5-HT3R antagonists in behavioral studies, however, has led to the view that this receptor participates in several pharmacological events, such as anxiolytic, antipsychotic, and cognitive-enhancing actions and facilitation of the withdrawal from drugs of abuse (Carboni et al., 1989; Costall et al., 1990, 1993; Nevins and Anthony, 1994). In addition, activation of 5-HT3R has been demonstrated to modulate the release of various neurotransmitters in the brain (Barnes et al., 1989b; Blandina et al., 1989; Chen et al., 1991, 1992; Paudice and Raiteri, 1991; Maura et al., 1992).

To obtain information on the functional significance of the 5-HT3R-containing neurons, we sought to determine additional neurochemical features of the neurons that express this receptor in the telencephalon.

MATERIALS AND METHODS

Tissue preparation. Twenty adult male Sprague Dawley 20 rats (100-120 gm body weight) were anesthetized with choral hydrate (3.5 mg/100 gm body weight) and perfused transcardially with a solution of 4% paraformaldehyde in 0.1 M phosphate buffer (PB), pH 7.3. Brains were post-fixed overnight, rinsed with PB, and sequentially transferred to 12%, 14%, and 16% sucrose solutions. Brains were then frozen on dry ice, and sections of 30-40 µm thickness were obtained on a cryostat.

Probe preparation. [35S]- and [33P]-labeled sense and antisense RNA probes were generated from a 730 basepair (bp) PstI insert (corresponding to nucleotides 1500-2230 of the rat 5HT3R cDNA), using the TransProbe T Kit (Pharmacia, Piscataway, NJ). The [35S]- and [33P]-labeled antisense probes were synthesized separately.

In situ hybridization-immunocytochemistry labeling. In situ hybridization combined with immunolabeling was performed as described previously (Morales et al., 1996a). Free-floating cryosections were incubated in PB supplemented with 0.5% Triton X-100 for 10 min, rinsed 2 × 5 min with PB, treated with 0.2N HCl for 10 min, rinsed 2 × 5 min with PB, and then acetylated in 0.25% acetic anhydride in 0.1 M triethanolamine, pH 8.0, for 10 min. Sections were rinsed 2 × 5 min with PB and post-fixed with 4% paraformaldehyde for 10 min, and after a final rinse with PB, sections were prehybridized for 3 hr at 55°C in hybridization buffer (50% formamide, 10% dextran sulfate, 5× Denhardt's solution, 0.62 M NaCl, 50 mM dithiothreitol, 10 mM EDTA, 20 mM PIPES, pH 6.8, 0.2% SDS, 250 µg/ml ssDNA, 250 µg/ml tRNA). After prehybridization, sections were hybridized at 55°C for 16 hr in hybridization buffer containing [35S]- and [33P]-labeled single-stranded RNA probes at 107 cpm/ml. Sections were treated with RNase A at 4 µg/ml at 37°C for 1 hr, washed in 1 × SSC, 50% formamide at 55°C for 2 hr, and in 0.1 × SSC at 68°C for 1 hr. Sections were rinsed with PB, incubated in 1% bovine serum albumin (BSA) supplemented with 0.3% Triton X-100 in PB for 1 hr, and then incubated with the corresponding primary antibody for 24 hr at 4°C. A previously well characterized rabbit polyclonal antibody against SS, SS-320 (Morrison et al., 1983), was used at dilution 1:3000 (antibody was provided by Dr. R. Benoit, Montreal, Quebec, Canada); anti-GABA rabbit antibody (Sigma, St. Louis, MO) was used at dilution 1:2000. A specific anti-cholecystokinin (CCK) monoclonal antibody raised in mouse was used at dilution 1:2000 (provided by Dr. D. Goodwillie, Farmitalia Carlo Erba, Nerviano, Italy), and anti-parvalbumin (PV), anti-calbindin (CB) mouse monoclonal, and anti-calretinin (CR) rabbit polyclonal antibodies were used at dilution 1:2000 (Swant).

After sections were rinsed 3 × 10 min in PB, they were processed with an ABC kit (Vector, Burlingame, CA). Material was incubated in a 1:200 dilution of the corresponding biotinylated secondary antibody. After they were rinsed with PB, sections were incubated with avidin-biotinylated horseradish peroxidase for 2 hr. Samples were rinsed, and the peroxidase reaction was developed with 0.05% 3,3-diaminobenzidine-4 HCl (DAB) and 0.003% hydrogen peroxide (H2O2). All antibody dilutions were performed in PB supplemented with 1% BSA and 0.3% Triton X-100. Sections were mounted on coated slides, air-dried, dipped in nuclear track emulsion, and exposed for several weeks before development. Material was photographed under bright-field or epiluminescence microscopy.

When hybridization was performed with sense probes, few silver grains were scattered on the sections. This level of signal was very low and was considered as unspecific background. In contrast, hybridization with antisense probes resulted in a localized signal distribution.

Double immunocytochemistry. Free-floating cryosections were processed as described under immunocytochemistry. Sections were incubated with a specific rabbit polyclonal anti-5-HT3R antibody (0165) at 1:4000 dilution for 24-48 hr at 4°C. We have established previously the specificity of the anti-5-HT3R antibody used in this study (Morales et al., 1996a). After the peroxidase reaction was developed with DAB and H2O2, the sections were rinsed with PB, and the remaining hydrogen peroxidase activity was inactivated by incubating sections in methanol containing 0.3% H2O2 for 15 min at room temperature. After several rinses with PB, sections were treated with an avidin solution (Vector) to block biotin groups for 1 hr at room temperature. Sections were incubated in the second primary antibody (anti-CCK at 1:2000 dilution) supplemented with biotin-blocking solution (Vector) and incubated for 24 hr at 4°C. After they were rinsed 3 × 10 min in PB, sections were processed with an ABC kit (Vector). Material was incubated in a 1:200 dilution of anti-mouse biotinylated secondary antibody. After sections were rinsed with PB, they were incubated with avidin-biotinylated horseradish peroxidase for 2 hr. Samples were rinsed with 0.1 M sodium phosphate buffer (SPB), pH 6.8, and the peroxidase reaction was developed in 0.01% benzidine dihydrochloride, 0.025% sodium nitroferricyanide, and 0.005% H2O2 in SPB (Levey et al., 1986), which results in a granular blue-black reaction product.

Data analysis. Sections processed for in situ hybridization and immunocytochemistry were analyzed and photographed with bright-field or epiluminescence microscopy. We performed a semiquantitative analysis of double-labeled cells in the neocortex. A neuron was considered double-labeled when its soma was brown and contained more than 15 silver grains. In pilot experiments we found that 15-20 silver grains represent weak label above background. Double-labeled cells were counted in three random sections of three different experiments, and the percentage of neurons containing both 5-HT3R mRNA and immunoreactivity was calculated from the total population of immunolabeled cells.

RESULTS

We have previously used in situ hybridization and immunocytochemistry to show the presence of GABA in neurons that express the 5-HT3R in the neocortex and hippocampus (Morales et al., 1996b). In the present study, we investigated further the neurochemical composition of 5-HT3R-expressing neurons in the telencephalon.

Colocalization of 5-HT3R transcripts and GABA

Coexistence of 5-HT3R transcripts with GABA immunoreactivity was observed in several areas of the telencephalon. These 5-HT3R/GABA neurons were often found in those brain areas that contained cells with high levels of 5-HT3R expression, such as the neocortex (Fig. 1A,B), olfactory regions, hippocampal formation, amygdaloid complex, and septal region (Fig. 2A,B). Double-labeled cells were also detected occasionally in areas in which neurons contained low levels of 5-HT3R mRNA, such as the corpus callosum, corpus striatum, thalamus, and hypothalamus. A semiquantitative analysis performed from three random sections of three different experiments indicated that between 70 and 95% of the 5-HT3R-expressing cells were GABAergic throughout the telencephalon (n = 550 cells).
Fig. 1. Simultaneous detection of 5-HT3R transcripts and GABA immunoreactivity in layer II of parietal cortex. A, Observation of 5-HT3R-expressing cells under epiluminescence microscopy. B, Observation of GABA-immunoreactive neurons under bright-field microscopy. In this section, all 5-HT3R-expressing cells showed GABA immunoreactivity (filled arrows in A and B), but not all GABA-immunoreactive neurons contained 5-HT3R transcripts (open arrows in B). Scale bar, 25 µm.
[View Larger Version of this Image (162K GIF file)]

Fig. 2. Simultaneous detection of 5-HT3R transcripts and GABA immunoreactivity in the medial septal nucleus. A, Observation of 5-HT3R-expressing cells under epiluminescence microscopy. B, Observation of GABA-immunoreactive neurons under bright-field microscopy. Double-labeled cells are indicated by arrows. Scale bar, 25 µm.
[View Larger Version of this Image (132K GIF file)]

The 5-HT3R/GABAergic neurons were found throughout the different layers of the neocortex but were preferentially concentrated in layers II-III and V-VI. This pattern of distribution was observed from the frontal to the occipital regions of the neocortex. Within the olfactory regions, 5-HT3R/GABAergic neurons were detected in the olfactory tubercle, piriform cortex, endopiriform nucleus, anterior olfactory nucleus, and taenia tecta. The 5-HT3R/GABAergic neurons of the hippocampal formation were distributed in all layers of the CA1-CA3 subfields of the hippocampus, dentate gyrus, hilus, and subiculum. The 5-HT3R/GABAergic cells of the amygdaloid complex were found mainly in the lateral, basolateral, basomedial, and cortical amygdaloid nuclei.

Colocalization of 5-HT3R transcripts and neuropeptides

Somatostatin (SS) and CCK are neuropeptides known to coexist with GABA in nonoverlapping neuronal populations in the cortex and hippocampus (Somogyi et al., 1984; Sloviter and Nilaver, 1987). Thus, we investigated whether either of these peptides was present in the 5-HT3R-expressing neurons. No SS immunoreactivity was found in any 5-HT3R-expressing neurons, although single SS-immunopositive or 5-HT3R-expressing neurons were identified readily in cortex and hippocampus. In contrast, CCK immunoreactivity was often observed in 5-HT3R-expressing neurons of the neocortex (Fig. 3A,B), olfactory regions (Fig. 3C,D), hippocampal formation, and amygdaloid complex.
Fig. 3. Simultaneous detection of 5-HT3R transcripts and CCK immunoreactivity in the temporal (A, B) and piriform (C, D) cortices. A, C, Observation of 5-HT3R-expressing cells under epiluminescence microscopy. B, D, Observation of CCK-immunoreactive neurons under bright-field microscopy. In these sections, several 5-HT3R-expressing cells showed CCK immunoreactivity (filled arrows), but not all CCK-immunoreactive neurons contained 5-HT3R transcripts (open arrows). Scale bar, 25 µm.
[View Larger Version of this Image (85K GIF file)]

Within the neocortex, 5-HT3R/CCK-labeled cells were distributed mainly in layers II-III, although colocalization was also observed in cells of deeper layers. These double-labeled neurons were found frequently in the motor, prelimbic, and visual cortices (see n in Table 1). A semiquantitative analysis indicated that between 35 and 65% of the CCK-containing cells in layers II-III of the neocortex (n = 726 cells) express the 5-HT3R (Table 1). We frequently observed 5-HT3R/CCK-labeled cells in the anterior olfactory nucleus and piriform cortex, and a semiquantitative evaluation of these cells showed that 55-65% of the CCK-immunoreactive cells (n = 147 cells) contained 5-HT3R transcripts (Table 1). These results indicate that the percentage of 5-HT3R/CCK-labeled neurons is not homogenous within the different cortical areas.

Table 1. Percentage of CCK-immunoreactive neurons expressing the 5-HT3R

Region Percentage of double-labled neuronsa
Neocortex
  Motor cortex 49  ± 0.47 (n = 119)
  Agranular insular cortex 35  ± 0.47 (n = 25)
  Anterior cingulate cortex 60  ± 0.47 (n = 62)
  Auditory cortex 36  ± 0.47 (n = 113)
  Posterior parietal association cortex 66  ± 0.47 (n = 55)
  Prelimbic cortex 55  ± 0.47 (n = 89)
  Somatosensory cortex 35  ± 0.47 (n = 70)
  Visual cortex 46  ± 0.47 (n = 193)
Olfactory system
  Anterior olfactory nucleus 65  ± 0.47 (n = 45)
  Piriform cortex 53  ± 0.47 (n = 102)
Entorhinal cortex 55  ± 0.47 (n = 75)
a Total number of CCK-immunopositive cells (n) was counted in three sections each from three different experiments, and the percentage of CCK-immunoreactive cells expressing the 5-HT3R was calculated.

5-HT3R/CCK-labeled cells were found scattered throughout the hippocampus in the stratum (st.) oriens, st. pyramidale, and st. radiatum of the CA1-CA3. Within the st. oriens, double-labeled cells were sometimes located near the alveus or close to the st. pyramidale. The 5-HT3R/CCK-labeled interneurons were located at the border of the granular cell layer in the dentate gyrus. Several nuclei of the amygdaloid complex also have 5-HT3R/CCK neurons, mainly the lateral, basolateral, basomedial, and cortical nuclei.

Colocalization of 5-HT3R and CCK

In another set of experiments, we used an anti-5-HT3R antibody (Morales et al., 1996a) for the simultaneous detection of 5-HT3R and CCK immunoreactivity. We found that some 5-HT3R-immunolabeled neurons were also positive for CCK immunostaining. The pattern of distribution of these double-immunostained cells parallels that observed for CCK-immunoreactive/5-HT3R mRNA-hybridized neurons in the neocortex and the hippocampal formation. Double-immunolabeled cells, however, were difficult to evaluate when the CCK immunoproduct appeared as sparse grains or dense aggregates in the cell bodies, limiting accurate quantitation of double-labeled cells.

Double 5-HT3R/CCK-immunoreactive neurons were scattered in all layers of the neocortex and occasionally were found in the white matter. Layers II-III contained the highest density of 5-HT3R/CCK-immunoreactive neurons, and immunoreactive CCK was observed in the perikarya and processes; however, the 5-HT3R immunoproduct was restricted mainly to the cell bodies.

Double 5-HT3R/CCK-immunolabeled perikarya were found in the st. oriens, st. pyramidale, st. radiatum, and st. lacunosum moleculare of CA1 (Figs. 4A, 5A,B) and CA3 (Fig. 4B) subfields. The 5-HT3R/CCK-labeled interneurons contained CCK-immunopositive processes extending from the st. oriens to the st. pyramidale and from the st. radiatum to the st. lacunosum moleculare (Fig. 5A). In addition, double 5-HT3R/CCK cell bodies located in the st. pyramidale of the CA1 subfield showed CCK-labeled processes extending into the st. pyramidale (Fig. 5B). Double 5-HT3R/CCK-immunolabeled perikarya were observed immediately below the granule cell layer of the dentate layer, with CCK-immunopositive processes entering the granule cell layer (Fig. 5C).

Fig. 4. Simultaneous detection of 5-HT3R and CCK immunoreactivity in the CA1 (A) and CA3 (B) subfields of the hippocampus. Colocalization of 5-HT3R (brown) and CCK (granular blue-black) immunoproducts was found in several neurons (arrows), but not all 5-HT3R-immunoreactive cells contained CCK immunoreactivity (arrowheads). Insets in A and B show double-labeled neurons at high magnification. SR, St. radiatum; SLM, st. lacunosum moleculare. Scale bar, 12.5 µm; for inset, 6 µm.
[View Larger Version of this Image (157K GIF file)]

Fig. 5. Simultaneous detection of 5-HT3R and CCK immunoreactivity in CA1 (A, B) and dentate gyrus (C). A, Note a 5-HT3R/CCK double-labeled cell in the SR extending into the SLM. B, A 5-HT3R/CCK double-labeled cell in the st. pyramidale (SP) extending into the cell layer. C, Two 5-HT3R/CCK double-labeled neurons immediately below the st. granulare (SG); one of them projects into the granule cell layer (arrow). SR, St. radiatum; SLM, st. lacunosum moleculare. Scale bar, 6 µm.
[View Larger Version of this Image (143K GIF file)]

Colocalization of 5-HT3R transcripts and Ca2+-binding proteins

GABAergic cells are known to contain different Ca2+-binding proteins, PV, CB, and CR (Baimbridge et al., 1992, for review). Thus, we sought to determine whether any of these proteins were present in 5-HT3R-expressing neurons. Although no PV immunoreactivity was found in 5-HT3R-expressing neurons, both CB and CR did colocalize with 5-HT3R-expressing neurons.

5-HT3R/CB double-labeled neurons were found occasionally in the entorhinal cortex and basomedial nucleus of the amygdala. In contrast, this type of neuron was often observed in basket cells of the dentate gyrus and the different strata of the hippocampus, preferentially in the st. radiatum of the ventral portion of the CA1 and CA3 subfields (Figs. 6), and a semiquantitative analysis of double-labeled cells indicated that between 33 and 63% of the CB-immunoreactive cells (n = 267 cells) expressed the 5-HT3R in these regions.

Fig. 6. Simultaneous detection of 5-HT3R transcripts and CB immunoreactivity in CA3. A, Observation of 5-HT3R-expressing cells under epiluminescence microscopy. B, Observation of CB-immunoreactive neurons under bright-field microscopy. In this section, the majority of 5-HT3R-expressing cells showed CB immunoreactivity (filled arrows in A and B), but a few CB-reactive cells were single-labeled for the protein (open arrows in B). Note a 5-HT3R-expressing cell without CB immunoreactivity (open arrow in A). Scale bar, 22 µm.
[View Larger Version of this Image (101K GIF file)]

5-HT3R/CR double-labeled neurons showed a wider distribution than those for CB. The 5-HT3R/CR-containing neurons were scattered throughout the forebrain: neocortex, olfactory tubercle, endopiriform nucleus, anterior olfactory nucleus, hippocampal formation, posteromedial cortical, and dorsolateral nuclei of the amygdala. 5-HT3R/CR double-labeled neurons, however, were concentrated preferentially in the motor (Figs. 7A,B) agranular, perirhinal, insular, and piriform cortices. Within the hippocampal formation, these neurons were distributed mainly in the st. lacunosum moleculare of the subiculum (Fig. 8A,B) and CA1-CA3 areas.

Fig. 7. Simultaneous detection of 5-HT3R transcripts and CR immunoreactivity in the occipital cortex. A, Observation of 5-HT3R-expressing cells under epiluminescence microscopy. B, Observation of CR-immunoreactive neurons under bright field. In this section, several of the 5-HT3R-expressing cells showed CR immunoreactivity (filled arrows in A and B), and few were single-labeled (open arrows in A and B). Scale bar, 25 µm.
[View Larger Version of this Image (105K GIF file)]

Fig. 8. Simultaneous detection of 5-HT3R transcripts and CR immunoreactivity field in the st. lacunosum moleculare of the subiculum. A, Observation of 5-HT3R-expressing cells under epiluminescence microscopy. B, Observation of CR-immunoreactive neurons under bright field. In this section, the majority of 5-HT3R-expressing cells showed CR immunoreactivity (filled arrows in A and B), and few were single-labeled (open arrows in A and B). Scale bar, 18 µm.
[View Larger Version of this Image (158K GIF file)]

DISCUSSION

Characterization OF 5-HT3R/GABA-expressing cells

It has been demonstrated that cortical and hippocampal GABAergic interneurons are a heterogeneous population with regard to their morphology, biochemical composition, and synaptic connections (Wolff and Chronwall, 1982; Freund et al., 1983, 1990; Hendry et al., 1984; Somogyi et al., 1984; Kosaka et al., 1985; Celio, 1986; Sloviter and Nilaver, 1987; Katsumaru et al., 1988). Various biochemical properties of the GABAergic interneurons have been widely used to distinguish subpopulations of GABAergic neurons in cortex and hippocampus (Hendry et al., 1984; Somogyi et al., 1984; Kosaka et al., 1985; Celio, 1986; Sloviter and Nilaver, 1987; Katsumaru et al., 1988; Freund et al., 1990). Subpopulations of GABAergic neurons can be identified according to their calcium-binding protein (i.e., PV, CB, and CR) or neuropeptide content (i.e., SS and CCK). Our data indicate that the 5-HT3R/GABA-expressing neurons belong to a distinct group of GABAergic cells that lack SS and PV but may contain CCK, CB, and CR. These three subgroups of 5-HT3R/GABAergic interneurons have a distinct regional distribution, which is consistent with observations from cortical and hippocampal interneurons showing that PV- and CB-containing neurons form two different populations of GABAergic neurons that do not coexist with CCK (Hendry et al., 1984; Hendry and Jones, 1985; Gulyás et al., 1991). These data support the view that the 5-HT3R-containing neurons are composed of a biochemically heterogeneous subpopulation of neurons that may be involved in different inhibitory circuits.

Coexistence of 5-HT3R and CCK within the same cells

Previous studies have shown that the vast majority of cortical and hippocampal CCK-containing neurons are GABAergic in the rat brain (Hendry et al., 1984; Somogyi et al., 1984; Kosaka et al., 1985). We found that the 5-HT3R, which is expressed in some interneurons, is present in a subpopulation of CCK-immunoreactive neurons in the neocortex, olfactory system, and hippocampal formation.

The colocalization of 5-HT3R and CCK immunoreactivity within some interneurons implies functional interactions between serotonergic terminals and CCK-containing cells. In addition, the findings that 35-66% of the CCK-containing cells in layers II-III of the neocortex and 55-65% of neurons of the anterior olfactory nucleus and piriform cortex express the 5-HT3R suggest that CCK neurotransmission is highly regulated by serotonin in the rat telencephalon. The presence, however, of putative serotonergic contacts on CCK cells has not yet been reported. In contrast, some neurochemical studies indicate that 5-HT3R activation is capable of mediating the release of CCK. Paudice and Raiteri (1991) demonstrated that serotonin or 1-phenylbiguanide, a selective 5-HT3R agonist, enhances the depolarization-evoked release of CCK from synaptosomes prepared from rat cerebral cortex, and this effect was prevented by 5-HT3R antagonists. The 5-HT3R antagonists also prevent the veratrine-evoked release of CCK in frontal cortex of freely moving rats (Raiteri et al., 1993), suggesting that activation of 5-HT3R on CCK-releasing nerve endings might mediate the release of CCK. These pharmacological studies did not show which of the two possible sources of CCK in cortex, an intrinsic source from local CCK-containing cells or an extrinsic one from projections of the mesencephalon, were the source of released CCK. Our data on the presence of 5-HT3R/CCK-containing neurons in cortex and hippocampus suggest that activation of local 5-HT3R/CCK-containing cells might participate in the release of CCK in cortex and hippocampus.

The activity of hippocampal 5-HT3R/CCK-containing neurons is likely to be regulated by the raphe nucleus. In addition, another source of regulation might be provided by the septum, because it has been reported that hippocampal CCK-containing neurons are innervated by GABAergic septal fibers (Gulyás et al., 1990), thus making the 5-HT3R/CCK-containing neurons the target of subcortical pathways such as those originating in the septum and raphe nucleus.

We also detected 5-HT3R/CCK double-immunolabeled neurons extending into the pyramidal and granule layers of the hippocampus and dentate gyrus. These results suggest that some of the 5-HT3R/GABA/CCK-containing neurons might inhibit principal neurons in these regions. In agreement with this suggestion, it is known that CCK-positive boutons establish symmetrical synaptic contacts with perikarya and dendrites of pyramidal and nonpyramidal neurons in hippocampus (Harris et al., 1985; Hendry and Jones, 1985; Nunzi et al., 1985; Totterdell and Smith, 1986), and that CCK immunoreactivity is found in axonal terminals contacting perikarya of neurons in the cortex (Köhler and Chan-Palay, 1982). The activation of 5-HT3R in CCK-containing interneurons might result in the direct inhibition of principal neurons, thus affecting areas to which these principal neurons project (Fig. 9). This inhibition might occur in pyramidal neurons of the subiculum that project to the nucleus accumbens and are innervated by CCK-immunoreactive terminals (Totterdell and Smith, 1986).

Fig. 9. Hypothetical circuitry involving serotonergic activation of 5-HT3R on inhibitory interneurons. Serotonin-immunoreactive terminals make asymmetrical synapses on dendrites of hippocampal and cortical interneurons (Freund et al., 1990; Smiley and Goldman-Rakic, 1996); some of these interneurons containing 5-HT3R and CCK (1) might be activated by 5-HT, resulting in inactivation of other interneurons (2) and principal neurons. It is known that CCK-positive boutons establish symmetrical synaptic contacts with perikarya and dendrites of pyramidal and nonpyramidal neurons in hippocampus (Harris et al., 1985; Hendry and Jones, 1985; Nunzi et al., 1985; Totterdell and Smith, 1986), and that CCK immunoreactivity is found in axonal terminals innervating the perikarya of neurons in the cortex (Köhler and Chan-Palay, 1982). The activation of 5-HT3R in CCK-containing interneurons (1) might result in the direct inhibition of principal neurons, thus affecting areas to which these principal neurons project.
[View Larger Version of this Image (20K GIF file)]

Coexistence of 5-HT3R with Ca2+-binding proteins

The characterization of 5-HT3R/GABAergic neurons on the basis of the content of their calcium-binding proteins suggests that these neurons lack PV but may contain CR and CB.

Some 5-HT3R/CR-containing cells were found in the neocortex, olfactory cortex, hippocampus, and amygdala, but these neurons were more often present in the agranular insular and piriform cortices. In contrast, the 5-HT3R/CB double-labeled neurons were found mainly in the CA1-CA3 subfields of the hippocampus. These results are consistent with previous observations showing that serotonergic median raphe axons selectively innervate the somata and dendritic trees of CB-containing GABAergic interneurons in the CA1 and CA3 subfields of the rat hippocampus, but never those that contain PV (Freund et al., 1990; Halasy et al., 1992; Hornung and Celio, 1992; Miettinen et al., 1992). Likewise, CR-immunoreactive neurons of the hippocampus also receive serotonergic synaptic contacts from the median raphe nuclei (Acsády et al., 1993). In addition, the hippocampal CB- and CR-immunoreactive neurons receive innervations from the medial septum (Freund and Antal, 1988; Freund et al., 1990; Gulyás et al., 1990; Acsády et al., 1993). The CB-containing interneurons are mainly basket and axo-axonic cells that make symmetrical synapses on the soma of the principal cells but also contact the axon initial segments, proximal dendrites, and dendritic spines of the pyramidal cells (Katsumaru et al., 1988; DeFelipe et al., 1989). The presence of CB and CR in 5-HT3R-expressing interneurons suggests that these interneurons might belong to those hippocampal CB- and CR-immunoreactive neurons shown previously to be innervated by subcortical inputs from the medial septal and median raphe nuclei. In addition, these 5-HT3R/CB and 5-HT3R/CR interneurons might modulate neuronal transmission of interneurons and principal neurons.

In the present study we demonstrated that the neuronal expression of the 5-HT3R is selective within the GABA neuron population in the rat telencephalon, extending preliminary observations showing that 5-HT3R transcripts coexist with GABA in the neocortex and hippocampus (Morales et al., 1996b). Despite the extensive colocalization of 5-HT3R transcripts and GABA in the same neurons throughout the telencephalon, the expression of the 5-HT3R is not restricted to GABAergic cells in the CNS; for instance, we have detected 5-HT3R mRNA in some dopaminergic neurons of the mesencephalon (M. Morales and F. E. Bloom, unpublished observations) and in motoneurons of the dorsal horn of the spinal cord (Morales et al., 1996a).

The widespread colocalization of 5-HT3R transcripts and GABA in the same neurons suggests the participation of 5-HT3R in the excitation of inhibitory neurons in several brain regions. Consistent with these observations, previous immunohistochemical studies have shown that serotonin-immunoreactive terminals make asymmetrical synapses on dendrites of hippocampal and cortical interneurons (Freund et al., 1990; Smiley and Goldman-Rakic, 1996). Furthermore, 5-HT directly excites GABAergic interneurons via 5-HT3R and consequently increases the frequency of inhibitory synaptic events recorded in CA1 pyramidal cells of rat hippocampal slices (Ropert and Guy, 1991). Although no similar electrophysiological findings have been reported in cortical areas, it is likely that some of the 5-HT3R/GABAergic cortical neurons will also inhibit cortical pyramidal neurons or interneurons. The enhancement of GABAergic inhibition by activation of 5-HT3R might be relevant for brain regulatory events, because 5-HT3R agonists will inhibit the induction of long-term potentiation (LTP) in CA1 and CA3 (Corradetti et al., 1992; Maeda et al., 1994) through the facilitation of GABAergic neurons (via GABAA receptors). In addition, intraperitoneal administration of the 5-HT3R antagonist ondansetron increased the frequency of the hippocampal theta rhythm, the induction of LTP in CA1, and the retention of olfactory and spatial memory in freely moving rats, suggesting that 5-HT3R blockage of GABAergic cells results in the disinhibition of pyramidal cells (Stäubli and Xu, 1995). These observations suggest that the 5-HT3R might participate in inhibitory and disinhibitory circuits in the rat telencephalon.

Relations to other 5-HT receptor localizations

The functional role played by the 5-HT3R we have localized to GABA-containing interneurons provides only one facet of what may be viewed as serotonergic synaptic functions in the cerebral cortex and hippocampal formation. Clearly, the 5-HT3R is only one of several 5-HT receptor subclasses whose cellular locations have already been assessed. In several recent studies on the cellular distribution of other 5-HT receptors, only the 5-HT2R (Molineaux et al., 1989; Morilak et al., 1993; Freund and Buzsaki, 1996) has been detected on interneurons in addition to the 5-HT3R; however, the 5-HT2R is also present in principal neurons (Morilak et al., 1993; Hamada et al., 1996). The other major subtypes of 5-HT receptors, such as the 5HT1AR (Gerard et al., 1994) and the 5HT6R (Miquel et al., 1996) have been attributed only to the pyramidal neurons of the hippocampus but were not detected in interneurons. Although such studies cannot define precise roles for the different 5-HT-transducing target neurons, the contrasting localization may provide the basis for developing hypotheses for future pharmacological analyses.

In conclusion, we demonstrated that the 5-HT3R-expressing neurons are mainly GABAergic in the rat telencephalon, suggesting that depolarization of these cells by serotonin might regulate inhibitory and disinhibitory circuits in the rat telencephalon. The 5-HT3R-expressing cells might contain CCK, CB, and CR. This biochemical heterogeneity may reflect the participation of these neurons in different inhibitory circuits. In addition, the high degree of coexistence between the 5-HT3R and CCK is indicative of the importance that the 5-HT3R has in regulating CCK neurotransmission in the neocortex.

FOOTNOTES

Received Oct. 1, 1996; revised Jan. 27, 1997; accepted Feb. 10, 1997.

  

This work was supported by National Institute on Alcohol Abuse and Alcoholism Grant AA 06420. We thank Drs. David Johnson and Steve Heinemann for the rat 5-HT3R-A cDNA clone, and Elena Battenberg for her role in early experiments.

Correspondence should be addressed to Marisela Morales, The Scripps Research Institute, Department of Neuropharmacology, SBR-1, 10550 North Torrey Pines Road, La Jolla, CA 92037.


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