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The Journal of Neuroscience, July 15, 1998, 18(14):5344-5353

Identification of the Dopamine D3 Receptor in Oligodendrocyte Precursors: Potential Role in Regulating Differentiation and Myelin Formation

Ernesto R. Bongarzone1, Sherrel G. Howard1, 2, Vilma Schonmann1, and Anthony T. Campagnoni1

1 Mental Retardation Research Center and Brain Research Institute and 2 Molecular and Medical Pharmacology, School of Medicine, University of California at Los Angeles, Los Angeles, California 90024

    ABSTRACT
Top
Abstract
Introduction
Materials & Methods
Results
Discussion
References

Expression of the dopamine D3 receptor (D3r) was found in primary mixed glial cultures from newborn brain and in the corpus callosum in vivo during the peak of myelination. Expression of the D3r mRNA, but not D2r mRNA, was detected as early as 5 d in vitro (DIV) by RT-PCR. Immunoblot studies revealed D3r protein was also expressed in the cultures. Double immunofluorescence analysis for the D3r and for surface markers of specific stages of oligodendrocyte development indicated that D3r expression occurred in precursors and in immature oligodendrocytes but not in mature oligodendrocytes (i.e., A2B5+ 007- 01- and A2B5+ 007+ 01- cells but not A2B5- 007+ 01+ cells). Confocal microscopic analysis indicated that D3r was associated with cell bodies and cell membranes but not with the processes emanating from cell somas. Immunohistochemistry of brain sections revealed the presence of D3r in some oligodendrocytes located mainly within the genu and radiato of the corpus callosum during the active period of myelination.

Treatment of cultures with 20 µM quinpirole led to decreased numbers of O1+ oligodendrocytes possessing myelin-like membranes as well as an increase in the number of precursors in 14 DIV cultures. This effect was prevented by the dopamine antagonist haloperidol. These results show that the D3r expression is not restricted to neurons but it is also expressed in differentiating oligodendrocytes before terminal maturation. It also suggests that dopamine or some other D3r ligand may play a role in oligodendrocyte differentiation and/or the formation of myelin by mature oligodendrocytes.

Key words: myelination; dopamine receptors; brain development; cell lineage; oligodendroglia; neurotransmitters

    INTRODUCTION
Top
Abstract
Introduction
Materials & Methods
Results
Discussion
References

The D2 subfamily of dopamine receptors (D2r, D3r, and D4r) contains seven transmembrane regions (Bunzow et al., 1988; Sokoloff et al., 1990; Van Tol et al., 1991), a feature commonly associated with the members of the superfamily of G-protein-coupled receptors (Sibley et al., 1992). Although the activation of D2r and D4r involves the inhibition of adenyl cyclase (Sibley et al., 1992), the second messenger system associated with D3r has remained elusive (Sokoloff et al., 1992). The D3r gene encodes a 446 amino acid protein with an overall homology of 52% with D2r, which increases to 75% in the transmembrane domains (Sokoloff et al., 1990). Recently, a shorter isoform of D3r (425 amino acids) has been described in the mouse, which appears to be pharmacologically active (Fishburn et al., 1993). The significance of these long and short active isoforms of D3r is still unknown, but their presence might be related to differential modulation of the dopaminergic system.

Like other neurotransmitter receptors, dopamine receptors are found within specific synaptic circuits in the CNS. The D3 receptor subtype is expressed earlier than other dopamine receptor subtypes. Fishburn et al. (1996) were able to demonstrate the presence of D3 receptor mRNA expression in mice as early as day 9.5 postconception (pc), whereas the D2 receptor subtype was not detectable before day 13.5 pc. The distribution and expression of D3r in the brains of many species have been reported, including mouse (Demotes-Mainard et al., 1996), rat (Levesque et al., 1992; Landwehrmeyer et al., 1993a; Larson and Ariano, 1995), and human (Landwehrmeyer et al., 1993b), and they vary greatly depending on the area in the brain (Richtland et al., 1995). As the brain matures, the regional distribution of D3r becomes more defined, overlapping little with the D2r, (Gehlert et al., 1992; Diaz et al., 1995). During postnatal development, D3r mRNA concentrates in the mesocorticolimbic complex, with the highest levels occurring in the nucleus accumbens, the Islands of Calleja, and the olfactory tubercle (Levesque et al., 1992; Landwehrmeyer et al., 1993a; Ariano and Sibley, 1994).

The finding that some neurotransmitter receptors are actively expressed before the development and establishment of cortical and subcortical synapses during early embryonic development has raised the possibility of alternative biological functions for these receptors, in addition to their well described participation in synaptic transmission (Mattson, 1988). We have recently reported the expression of the D2r in oligodendrocytes (Howard et al., 1998), suggesting a possible nonsynaptic function for dopamine in these myelin-forming cells.

The purpose of this study was to examine the expression of the D2 receptor subfamily in oligodendrocytes in vitro and in vivo. Primary glial cultures have been well characterized and have proven to be excellent models for examining the developmental expression of genes important for oligodendrocyte survival and differentiation (Temple and Raff, 1985; Gard and Pfeiffer, 1990; Cameron and Rakik, 1991; Barres et al., 1993). A large array of stage-specific markers is available with which to correlate gene expression and differentiation state. Mature oligodendrocytes within these cultures elaborate myelin-like membrane sheets providing a good in vitro model for examining one of the major functions of this cell type. In this communication, we describe the identification of D3r in immature oligodendrocytes in vitro and in oligodendrocytes in vivo during the period of major myelin deposition, and we provide evidence that D3r may modulate the timing of oligodendrocyte maturation and subsequent elaboration of myelin sheaths.

    MATERIALS AND METHODS
Top
Abstract
Introduction
Materials & Methods
Results
Discussion
References

Tissue collection and cell culture. Brain cortices and olfactory tubercle and Island of Calleja areas collected from postnatal day 7 (P7) mouse brains were immediately frozen at -80°C. The procedure for culture of glial cells has been described in detail elsewhere (Bongarzone et al., 1996). Briefly, newborn (0- to 3-d-old) mouse cerebral hemispheres were dissociated through a nylon mesh, and cells were collected in 50 ml of 10% fetal calf serum DMEM. The cell suspension was poured through two collector tissue sieves (230 and 140 µm pore size) and centrifuged for 5 min at 100 × g. The cells, 15 × 106, were resuspended in 10 ml of medium and plated on 75 cm2 culture flasks previously coated with poly-L-lysine and incubated at 37°C 5% CO2, with changes of medium every 4 d. Pellets of glial cells were prepared after 5, 7, 9, 11, 14, and 18 d in vitro (DIV) and immediately frozen in liquid nitrogen. Primary cultures were also grown on poly-L-lysine-coated glass coverslips and fixed at the time points mentioned by incubation with 4% paraformaldehyde in 1× PBS for 20 min at room temperature. Six-day-old primary cultures were treated with quinpirole, haloperidol, or the combination of both drugs, with changes of drug-containing medium every 2 d. Cells were collected for RNA isolation after 8 d of treatment (14 DIV after cell plating). In some cases, glial cells were grown on glass coverslips and then were fixed and processed for immunocytochemistry as described below. In other experiments, treatment was canceled 6 DIV after initiation, and cells were maintained in drug-free medium for another 4 DIV before being fixed and processed for immunocytochemistry.

Extraction of RNA, cDNA synthesis, and RT-PCR reactions. Brain tissue or cell pellets were homogenized in an appropriate volume (100 mg of tissue/ml) of Trizol reagent (Life Technologies, Gaithersburg, MD) at 20,000 rpm for 30 sec (Polytron). Samples were incubated for 30 min at room temperature before extraction with chloroform for 5 min. After centrifugation at 7000 rpm at 4°C, the aqueous phase was transferred to a new tube, and total RNA was precipitated with isopropanol at -20°C for a minimum of 1 hr. RNA was washed in 75% ethanol, redissolved in DEPC-treated water at a final concentration of 5 mg RNA/ml, and stored at -80°C.

First-strand cDNA was synthesized by priming 20 µg of total RNA with an oligo-dT15 and extension with Superscript reverse transcriptase (Life Technologies) at 42°C for 60 min. Two microliters of the first-strand cDNA preparation were used as template in PCR reactions with the following specific sense and antisense oligonucleotide primers: (1) D35, 5'-CCCTGTCCTACTGTGCACTCATC; and D33, 5'-ATAGAATCTTGAGGAAGGCTTTG; these primers direct the amplification from nucleotide 97 to 1333 for the mouse D3r cDNA (Fishburn et al., 1993); and (2) D25, 5'-GGCGCCCTATGGCTTGAAGAG; and D23, 5'-CCTAGGCAGGGAGGCGGCAAG; these primers direct the amplification from nucleotide 78 to 1512 for the mouse D2r cDNA (Montmayeur et al., 1991). PCR conditions were 35 cycles, denaturation at 94°C for 1.5 min, annealing at 63°C (D3r) or 64°C (D2r) for 1.5 min, and extension at 72°C 1.5 min. PCR products were analyzed in a 1.5% agarose gel.

Cloning and sequencing. PCR fragments were cloned into the pCR 2.1 sequencing vector (Invitrogen, San Diego, CA). Sequence analysis was performed on cloned DNA strands by the Sanger dideoxy chain termination method (Sambrook et al., 1989) with [alpha -35S]dATP (DuPont NEN, Boston, MA) using one unit of Sequenase version 2.0 (United States Biochemicals, Cleveland, OH) per reaction.

Southern blot. Ten microliters of D3 RT-PCR-amplified products were separated by electrophoresis in a 1.5% agarose gel and transferred to a nylon membrane as described by Sambrook et al. (1989). After baking the membrane at 80°C for 2 hr, DNA was cross-linked by UV irradiation. The blot was prehybridized with hybridization solution [2× SSC (1 ×SSC = 150 mM NaCl and 15 mM sodium citrate, pH 7.2), 1× Denhardt's solution, 0.1 mg/ml sheared salmon sperm DNA (SS-DNA), 100 mM Tris-HCl, pH 7.6, and 1% SDS] without the probe at 55°C for 2 hr. A specific oligonucleotide primer (5'-ATTTCAGCCGCATTTGCTGTG), whose sequence corresponds to nucleotides 289-310 from the D3r cDNA, was end-labeled with [gamma -32P]dATP in the presence of T4 nucleotide kinase for 30 min at 37°C. The probe was further purified from nonincorporated radioactivity by ethanol precipitation. Hybridization (3 × 106 cpm/ml) was performed at 55°C for 18 hr. Blot was washed (1× SSC and 1% SDS at 37°C) and exposed for 30 min at room temperature.

In situ hybridization. Sense and antisense oligonucleotide primers (same used for RT-PCR) specific for D3r sequence were end-labeled with digoxigenin (DIG)-UTP as recommended by the manufacturer (Boehringer Mannheim, Indianapolis, IN). Fixed cultures were treated for 10 min with PBS containing 0.3% Triton X-100. Samples were washed twice (5 min each) with PBS and then incubated for 10 min at 37°C with 100 mM Tris-HCl and 50 mM EDTA, pH 8.0, containing 10 µg of RNase-free proteinase K/ml. Samples were rinsed twice in 2 mg/ml glycine in PBS and post-fixed for 10 min in 0.1% glutaraldehyde and 2% paraformaldehyde in PBS. Samples were then prehybridized for 2 hr at 42°C with hybridization buffer (50% deionized formamide, 1× Denhardt's solution, 4× SSC, 1% SDS, 0.1 mg/ml yeast tRNA, and 0.1 mg/ml SS-DNA) before hybridization for 18 hr at 42°C in this solution containing 100 ng/ml DIG-labeled primer. Samples were washed at 40°C in 50% formamide and 1× SSC and at room temperature in 1× SSC and then blocked with 5% normal goat serum in Tris-buffered saline (TBS). Bound DIG probe was detected with anti-DIG antibodies (1:500). Preparations were washed in TBS and then incubated with nitroblue tetrazolium and X-phosphate as recommended by the manufacturer (Boehringer Mannheim). Color reaction was developed overnight in the darkness and stopped by rinsing the samples in 10 mM Tris-HCl, 1 mM EDTA, and distilled water. Slides were mounted in Aquamount (Pittsburgh, PA).

Immunoblot. Membrane associated proteins were extracted with 0.1% Triton X-100 in the presence of a mixture of protease inhibitors, and 100 µg was electrophoresed in a 12% acrylamide-SDS gel (Laemmli, 1970). Proteins were electrotransferred onto nitrocellulose membranes (Towbin et al., 1979), and blots were incubated with 5% nonfat milk (Carnation) in TBS for 1 hr at room temperature. Polyclonal antibodies that recognize the C terminus of the D3r were diluted 1:1000 in 0.5% blocking solution and incubated with the blots for 3 hr at room temperature. After washing the membranes with fresh 0.01% Tween 20 in TBS, goat anti-rabbit IgG antibodies labeled with horseradish peroxidase were diluted 1:1500 and incubated with the blots for 1 hr at room temperature. Immunocomplexes were visualized by enhanced chemiluminescence (Pierce, Rockford, IL).

Immunocytochemistry. Fixed cells were permeabilized by incubation with 0.01% Triton X-100 in PBS for 1.5 min at room temperature. Coverslips were blocked with 5% normal sheep serum (NSS) in PBS at room temperature for 2 hr. Samples were then incubated with the following primary specific antibodies diluted in 0.5% NSS and 0.01% Tween 20 in PBS: goat polyclonal D3r anti-C terminus, 1:800 (Santa Cruz Biotechnology, Santa Cruz, CA); mouse monoclonal anti-A2B5, 1:50 (American Type Culture Collection, Manassas, VA); mouse monoclonal anti-galactocerebroside (O1), 1:100 (Dr. A. Gard, Department of Structural Biology, University of South Alabama); mouse monoclonal anti-OO7, 1:100; monoclonal mouse anti-neurofilament, 68 kDa, 1:100 (Chemicon, Temecula, CA); and rabbit polyclonal anti-neuron-specific enolase, 1:2000 (Chemicon). The first incubation was performed at 4°C for 18 hr. Samples were rinsed thoroughly with PBS and incubated with a 1:800 dilution of the appropriate secondary antibodies labeled with fluorescein or rhodamine (Boehringer Mannheim) for 2 hr at room temperature. Samples were then rinsed in PBS and then mounted on glass slides with Aquamount. Preparations were observed by epifluorescence with a Leica DMR microscope and by confocal microscopy using a Zeiss LSM confocal microscope.

Immunohistochemistry. BALB/c mice at P3, P9, P14, and P25 and adult animals were profoundly anesthetized using halothane, perfused using a chilled sterile solution of PBS, and then fixed with 2% paraformaldehyde in PBS. Brains were dissected out and post-fixed overnight in 4% paraformaldehyde in PBS. Tissue was cryoprotected with OCT, frozen, and then used to cut coronally oriented sections. Sections were processed for immunohistochemistry using the free-floating technique. Endogenous peroxidase activity was quenched by incubating the sections in 0.1% H2O2 in PBS for 15 min. Tissue was then blocked in 10% normal rabbit serum (NRS) in PBS for 2 hr at room temperature. Sections were incubated with goat anti-D3r antiserum (Santa Cruz), diluted 1:600 in 1% NRS and 0.02% Tween-20 for 72 hr at 4°C. Bound primary antibody was detected using the avidin-biotin-peroxidase system as recommended by the manufacturer (Vector Laboratories, Burlingame, CA). After development with diaminobenzidine, nickel ions, and H2O2, sections were mounted on gold-plus slides (Fisher Scientific), air-dried, dehydrated with serial graded ethanol, clarified in xylenes, and coverslipped with Permount.

    RESULTS
Top
Abstract
Introduction
Materials & Methods
Results
Discussion
References

Glial cells express the D3r in 14 DIV primary cultures

We analyzed whether cultured oligodendrocytes might express the principal members of the D2 subfamily of dopamine receptors during their in vitro differentiation. Glial cell cultures were established from newborn mice (P0-P3) and maintained for 2-3 weeks. Under the experimental conditions described in this study, any contaminating neurons, a potential source of dopamine receptors, did not survive. This was confirmed by the absence of immunoreactivity to the neuronal 68 kDa neurofilament and neuron-specific enolase (data not shown).

Glial cells were harvested at 7, 14, and 18 DIV, and their mRNAs were reverse-transcribed and used in PCR experiments designed to specifically detect the presence of either the D3r or the D2r mRNA. Primers D35 and D33 were chosen for the amplification of the long (1236 bp) and the short (1183 bp) isoforms of D3r (Fig. 1A), and primers D25 and D23 were selected to amplify the long (1404 bp) and the short (1346 bp) isoforms of the D2r (Fig. 1A). Both isoforms of the D2r were easily detected in the P7 mouse brain sample (Fig. 1B). In contrast, neither D2r isoform was detected in 7 or 14 DIV primary glial cultures (Fig. 1B) or in 18 DIV preparations (data not shown). The long isoform of the D3r, but not the short isoform, was amplified from P7 mouse brain (Fig. 1B). Interestingly, an intense band corresponding to the long D3r mRNA was detected in both 7 and 14 DIV primary cultures of mouse glia (Fig. 1B). The same result was obtained with a sample from 18 DIV primary cultures.


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  		Figure 1.   Primary cultures of glia express the D3r but not the D2r mRNA. The message for both dopamine receptors was detected by RT-PCR analysis. A, The scheme illustrates the structural organization of the D2r and D3r genes and their mRNAs. Two sets of specific oligonucleotide primers (open arrows, sense primers; filled arrows, antisense primers) were designed to amplify the cDNAs containing the open reading frame sequence for these receptors. B, Ethidium bromide staining of RT-PCR fragments. The mRNAs for both isoforms of the D2r were detected in a P7 brain sample, although no expression of this receptor was evidenced by RT-PCR in the glial samples at 7 or 14 DIV. The 1236 bp cDNA for the long D3r was readily amplified from the P7 brain sample. A single band with the same relative size was also detected in RNA samples from 7 and 14 DIV primary cultures of glia.

To confirm the identity of the putative long D3r detected in the glial cultures, PCR fragments were subcloned and sequenced, and the products were found to be 100% homologous with the mouse long D3r (Fishburn et al., 1993).

Process-bearing cells express the D3r mRNA

Our primary cultures of glia are composed of a mixture of astrocytes (~70%), oligodendrocytes (~20%) and microglia (~10%). Thus, the mRNA encoding the long D3r might have arisen from any of these cell types. To determine the cellular origin of the D3r expression, an in situ hybridization analysis was performed with specific D3r sense and antisense oligonucleotides labeled with digoxigenin. Figure 2 shows the results of such an experiment performed with a D3r antisense (Fig. 2B) and a control sense probe (Fig. 2A). With the antisense probe a strong, specific signal could be detected primarily within process-bearing cells of the size and morphology of cells in the oligodendroglial lineage. No labeling of astrocytes was evident.


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  		Figure 2.   D3r mRNA is produced in oligodendroglial-like cells in culture. D3r message was detected in 14 DIV primary cultures of mouse glia by in situ hybridization using an antisense oligonucleotide labeled with digoxigenin specific for the 3' end of exon 6 of D3r (B). Note that only cells with the morphology described for oligodendrocytes were detected. The staining is restricted to only the oligodendroglial cell bodies, and no message was evidenced within the cellular processes. The astrocytic layer (lining underneath the oligodendrocytes) was barely stained, and it is comparable to the nonspecific staining using a sense oligonucleotide (A). Scale bar, 20 µm.

Developmental expression of the D3r begins around 5 DIV in primary cultures of glia

We analyzed changes in the expression of the D3r mRNA with time in culture. The D3r mRNA was reverse transcribed and amplified by PCR at different stages over a period of 2 weeks after plating. The PCR fragments were identified by Southern blot using a D3r-specific radiolabeled oligonucleotide. A single band with the predicted size of ~1.2 kb, corresponding to the long isoform of the D3r, was detected in the control RNA sample isolated from P7 mouse brain (Fig. 3A). A similar band was also identified in all the RNA samples isolated from primary glial cultures, ranging from 5 to 14 DIV (Fig. 3A). Although not a quantitative analysis, the experimental conditions for the RT-PCR were strictly maintained for all the samples, and there were no evident changes in the intensity of the bands, suggesting that the expression of the D3r mRNA is maintained at similar levels over the first 2 weeks of culture.


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  		Figure 3.   The in vitro expression of D3r seems to follow a developmentally regulated pattern. A, By Southern blot analysis using a specific radiolabeled internal oligonucleotide, D3r message was identified in RT-PCR samples generated from P7 brain and glial primary cultures at 5, 9, 11, and 14 DIV. B, By immunoblot detection, D3r proteins were detected in the membrane extracts from the olfactory tubercle isolated from P7 brain as a 49 kDa product (possibly the nonglycosylated form of the receptor) and a 70 kDa product (possibly the glycosylated form of the receptor). D3r proteins were also immunodetected in membrane proteins extracted from primary cultures at 5, 9, 11, and 14 DIV.

We investigated whether the D3r mRNA was translated into protein by Western blot analysis. Using an antibody specific to the C terminus of the D3r protein, we were able to detect the immature, presumably nonglycosylated, state of the D3r with the predicted relative molecular weight of 49 kDa in a control membrane extract from the olfactory tubercle area of P7 mouse brain (Fig. 3B). A high molecular weight band (~70 kDa), presumably the fully glycosylated version of the receptor, was also immunodetected by the antibodies. The same basic pattern of the 49 and 70 kDa proteins was identified in membrane protein extracts isolated from primary cultures (Fig. 3B). Interestingly, the level of D3r protein produced in glial cultures seemed to increase as the cultures aged, appearing to reach a peak of expression between 9 and 11 DIV and then decreasing slightly at 14 DIV (Fig. 3B). The bands on the Western blots between 49 and 70 kDa presumably represent D3r products with varying levels of glycosylation.

D3r is expressed primarily in immature oligodendrocytes and is localized in the plasma membrane and cell bodies but not in the cell processes

Oligodendrocyte differentiation in vitro has been examined extensively with the use of stage-specific markers. Through the use of double immunocytochemistry, we were able to study the coexpression of D3r with early and late markers of oligodendrocyte differentiation. At 7 DIV, oligodendrocyte precursors could be identified with markers such as A2B5 and GD3. At 7 DIV, oligodendrocyte precursors were stained with the monoclonal antibody A2B5 (Fig. 4B, red fluorescence). All the A2B5+ oligodendrocyte precursor cells were found to express D3r (Fig. 4B, arrows, green fluorescence). In these cells, the D3r immunoreactivity was found associated with cell bodies, and little immunoreactivity was detected in processes. At later stages (14 DIV) some oligodendrocytes that had reached the stage of myelin membrane formation were easily observed by staining with the mature oligodendrocyte marker O1. As seen in Figure 4D these O1+ cells (red fluorescence) elaborated flattened, myelin-like sheets. The somas of these O1+ cells stained only very weakly, if at all, with the anti-D3r antibody (Fig. 4D, green fluorescence). At this stage there were many O1- D3r+ cells with the morphological characteristics of immature oligodendrocytes (Fig. 4D, arrows).


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  		Figure 4.   D3r colocalizes with A2B5+ but only occasionally with O1+ cells. Primary cultures were fixed at 7 DIV (A, B) and 14 DIV (C, D) and were analyzed by double immunofluorescence to detect D3r (green) with A2B5 (B) and O1 (D) (red). A, C, Phase-contrast micrographs corresponding to B and D, respectively. D3r colocalized with the early oligodendroglial marker A2B5 in all progenitor cells (B, arrows). At later stages in differentiation, oligodendrocytes that have started the synthesis of myelin membranes (evidenced with the monoclonal antibody O1; D) were faintly stained in their soma with the antibody against D3r (D). However, many D3r+ O1- cells without visible processes were also observed (D, arrows). Scale bars: B, 15 µm; D, 20 µm.

In these immunocytochemical studies, D3r was consistently observed to be associated with the cell bodies of the oligodendrocyte precursors, and very little signal was observed within their processes. To illustrate this differential localization more clearly, Figure 5 shows the subcellular distribution of D3r (red fluorescence) in 9 DIV primary cultures of glia doubly stained with 007 antibodies (green fluorescence) analyzed by confocal microscopy. The 007 is a surface marker that stains cells that are more differentiated than oligodendrocyte precursors, including immature and mature oligodendrocytes. As Figure 5 shows, D3r was observed in association with the cell bodies and cell membrane of the 007+ cells but the processes of these cells (stained in green with 007) did not stain for D3r protein. Taken together, the double immunocytochemical studies indicate that D3r is expressed primarily in oligodendrocyte precursors and immature oligodendrocytes but not in mature oligodendrocytes. Furthermore, D3r does not appear to localize in the cell processes of these cells.


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  		Figure 5.   D3r was detected in association with the plasma membrane but not with the cellular processes in differentiating oligodendrocytes. The subcellular localization of the D3r was studied in double immunofluorescence (D3r, red; OO7, green) staining of 9 DIV primary cultures by confocal microscopy. D3r was detected in association with the plasma membrane of oligodendrocytes, which could be clearly evidenced with the OO7 antibody. D3r immunoreaction was also observed within the cytoplasm of these cells. A network of cellular processes was easily visualized with OO7; however, no D3r immunoreaction was detected in association with them. Scale bar, 20 µm.

D3r is transiently expressed in oligodendrocytes located within the corpus callosum during the period of myelination

We wanted to determine whether oligodendrocytes in vivo expressed the D3r. To do this we performed immunohistochemical studies with a D3r-specific antibody during the early postnatal period of brain development, just before maximal myelination in the brain. A number of brain structures showed immunodetectable levels of the receptor. During the developmental period examined, D3r expression was noted in large neurons located in the mesocorticolimbic area. Highest expression was observed in the Island of Calleja and olfactory tubercle. These areas showed the earliest expression of D3r (P3) that was maintained throughout the developmental period examined. Figure 6B shows a representative field of a coronal section at the level of Island of Calleja of a P14 mouse brain in which D3r was localized in large clustered neurons of ~20 µm in diameter. D3r was also found expressed in pyramidal cells within layers 3 and 5 of the prefrontal cortex and in a lower level in the nucleus accumbens (data not shown).


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  		Figure 6.   D3r was detected in differentiating oligodendrocytes in the corpus callosum. The anatomical distribution of D3r was examined by immunohistochemistry in brain sections during the first 4 postnatal weeks and in the adult brain. A, Nonspecific staining of P14 corpus callosum using nonimmune serum. B, Mesocorticolimbic large-sized neurons from Islands of Calleja were heavily immunostained with the anti-D3r antibody. C-E, Micrographs showing a portion of the genu corpus callosum immunostained with the anti-D3r antibody at P3 (C), P14 (D), and P25 (E). Cells with small somal diameters (~7 µm) arranged in "strings" (arrowheads) or scattered individually throughout the white matter (arrows) were evident at P14 and to a lesser extent at P25. Scale bars: B, 20 mm; C-E, 10 mm.

To determine whether cells in the oligodendrocyte lineage expressed D3r, we focused our analysis on white matter areas, particularly in the corpus callosum in which >90% of the cells are oligodendrocytes. Cells with small cell bodies stained with the anti-D3r antibody in fiber tracts located adjacent to the neocortex. These cells had small somal diameters (~7 µm) with the "string of pearls" orientation characteristic of interfascicular oligodendrocytes (Peters et al., 1991). In addition to these, cells of similar morphology and size were scattered individually throughout the white matter tracts (Fig. 6). At P3 very little immunostaining could be observed in these cells (Fig. 6C). However, at later stages of neural development (beginning at P9; data not shown), D3r was evident within these oligodendroglial cells, with a peak of expression observed at P14, approximately 1 week before maximal myelination in this area (Fig. 6D). In the adult brain, white matter was barely immunoreactive, showing very few D3r+ cells in a scattered distribution throughout the fiber tracts (data not shown). These cells were of the size and morphology of small neurons. During development, D3r+ oligodendroglia was located almost exclusively in the genu of the corpus callosum, and some D3r+ cells were observed entering the radiato of the corpus callosum. No staining was evident in the external capsule or the anterior commissure. It is important to note that in all the cells analyzed, D3r was always associated with the cell bodies, and there was no immunostaining of the processes. Satellite and perivascular oligodendrocytes and astroglia did not show expression of the receptor at any stage of development.

Treatment of glial cultures with the dopamine agonist quinpirole altered the normal pattern of oligodendrocyte differentiation

It was of interest to determine whether the presence of the D3r had any functional significance for the oligodendrocyte. Accordingly, we examined whether quinpirole, a dopamine D2r/D3r agonist, had any effect on oligodendrocyte differentiation and/or myelin formation in the glial cultures. In vitro differentiation of quinpirole-treated primary cultures was evaluated by counting the number of A2B5+ cells, O1+ cells, and O1+ cells bearing myelin-like sheets in 14 DIV primary cultures. A2B5 was used as a marker for oligodendrocyte precursor cells, and O1 was used as a marker for mature oligodendrocytes. As shown in Figure 7A, there is normally a drop in the number of A2B5+ cells as the cultures mature. In this case the number of A2B5+ cells decreased from 21 cells per field at 7 DIV to 9 cells per field at 14 DIV. Treatment of the primary cultures with the agonist quinpirole resulted in an increase in the number of A2B5+ cells in the 14 DIV cultures from 9 to 14 cells per field. To be certain that the effect of quinpirole was mediated through D3r, parallel primary cultures were treated with quinpirole and the dopamine antagonist haloperidol. Under these conditions, the antagonist blocked the effect of the agonist, with the number of A2B5+ cells reaching levels similar to the untreated control at 14 DIV (Fig. 7A). Treatment with quinpirole had little effect on the number of oligodendrocytes at 14 DIV (Fig. 7A).


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  		Figure 7.   Stimulation of D3r by quinpirole decreased the in vitro differentiation of oligodendrocytes. A, The in vitro differentiation of oligodendrocytes was analyzed by counting the number of immature A2B5+ cells remaining in 14 DIV cultures and compared with the respective number in cultures at 7 DIV. The activation of D3r by quinpirole induced an increase of the number of A2B5+ cells in 14 DIV primary cultures with respect to the untreated control. This effect was blocked by coincubation with the antagonist haloperidol. Data are presented as the number of A2B5+ cells counted per field (0.1 mm2). At least 10 fields were observed from three separated experiments. Results were analyzed by the one-way ANOVA test (p < 0.05). The number of oligodendrocytes present at 14 DIV (analyzed by the presence of the marker O1) was also counted and analyzed as described previously. B, In vitro differentiation of oligodendrocytes was also evaluated by counting the number of O1+ cells connected to myelin-like membranes in 14 DIV (condition 1) and 16 DIV (condition 2) primary cultures. In condition 1, quinpirole or haloperidol treatments were begun at 6 DIV and continued until 14 DIV, and cultures were analyzed. Under this condition quinpirole induced a marked decrease (46%) of O1+ cells bearing myelin sheets compared with the untreated or haloperidol controls. In condition 2, quinpirole or haloperidol treatments were begun at 6 DIV, the drugs were removed at 12 DIV, and the cultures were analyzed at 16 DIV. Quinpirole treatment for day 6 appeared to be sufficient to reduce the numbers of sheet-bearing cells at 16 DIV with no apparent recovery after removal of the drug. Data represent the average of three separated experiments in triplicate and were analyzed by the one-way ANOVA test (p < 0.05). C, D, Immunofluorescence analysis of O1+ cells associated to myelin-like membranes in 14 DIV control (C) and quinpirole-treated (D) primary cultures. Observe the lesser extension of myelin-like membranes in the treated samples (D). Scale bar, 25 µm.

Interestingly, agonist stimulation of D3r led to a 46% decrease in the number of O1+ oligodendrocytes bearing myelin sheets compared with untreated controls (Fig. 7B). Furthermore, the presence of haloperidol antagonized the effect of quinpirole, increasing the number of oligodendrocytes bearing sheets to 92% of the untreated control. To investigate whether the effect of D3r on the formation of myelin membranes was irreversible, primary cultures were treated for 6 DIV and then switched to a drug-free medium for another 4 DIV before O1+ cells bearing myelin-like membranes were counted. Interestingly, the number of quinpirole-treated oligodendrocytes associated with myelin sheets (51%) did not return to normal levels 4 DIV after withdrawal of the drug (Fig. 7B). However, the antagonizing effect of haloperidol increased the number of cells associated with myelin sheets to 92% of the untreated control (Fig. 7B). The dose of drugs used in these studies (20 µM) did not produce any degeneration of the cultures and was substantially lower than that needed to cause a lethal effect on glial cells (data not shown). Figure 7, C and D, illustrates the differences in membrane sheet formation between oligodendrocytes in the quinpirole-treated cultures (Fig. 7D) and untreated cultures (Fig. 7C). Larger numbers of oligodendrocytes in the control cultures elaborated more extensive areas of myelin sheets at 14 DIV than in the quinpirole-treated cultures.

    DISCUSSION
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Abstract
Introduction
Materials & Methods
Results
Discussion
References

Our results show the expression of the D3r in immature oligodendrocytes in mouse primary glial cultures. We saw little or no expression of the D3r in mature oligodendrocytes. Interestingly, we were able to detect the D3r mRNA easily at 5 DIV, a time when immunoreactive protein on Western blots was detected at very low levels. Expression of the D3r in cells in the oligodendrocyte lineage was confirmed in vivo, particularly in cells within the corpus callosum before the period of maximal myelination in the mouse brain.

Our finding that the D3r is expressed in immature oligodendrocytes represents the third neurotransmitter receptor system to be identified in oligodendrocytes and their precursors. Gallo et al. (1989, 1996) have described the properties of the glutamate receptors in oligodendrocytes, and expression of the GABAA receptor has been reported in oligodendrocytes (Kettenmann, 1989; Von Blankenfeld et al., 1991). These results indicate that these neurotransmitter receptors are involved in processes other than nerve conduction in oligodendrocytes, and they raise the interesting question of what their functions might be in these glial cells. The concept that classical neurotransmitters may have additional, perhaps trophic, functions in glial and neuronal differentiation has been under consideration for some time and for which there is substantial precedent in the literature (for review, see Lauder, 1993).

Gallo et al. (1996) have presented evidence that glutamate receptors might be involved in oligodendrocyte progenitor cell proliferation. In this study, we found that quinpirole (a D2/D3 dopamine agonist) caused an increase of ~50% in the numbers of oligodendrocyte precursors present in 14 DIV cultures. Furthermore, treatment with quinpirole significantly reduced the numbers of oligodendrocytes elaborating membranous sheets, generally taken to represent oligodendrocytes at their most differentiated state. These results suggest that dopamine or some other endogenous D3r ligand, acting through this receptor, could prevent the oligodendrocyte from myelinating prematurely by delaying subsequent myelin sheath elaboration. The effect of the agonist seems to be irreversible, because relatively brief exposure to the drug is sufficient to decrease sheet-bearing cells in vitro. The extent to which dopamine or some other D3r ligand might influence myelination in vivo remains to be determined.

Expression of the D3r in cells in the oligodendrocyte lineage was noted only in the corpus callosum during a developmental stage at which oligodendrocytes are beginning to elaborate processes and ensheathing axons. As brain maturation occurred, the expression of D3r in these cells was found to decrease. In the adult nervous system, only scattered cells were labeled in the corpus callosum, which correlates well with the observed absence of D3r expression in fully mature oligodendrocytes in vitro.

Although innervation by the dopamine system is somewhat restricted, anatomically there are three well defined dopamine systems in the adult brain. The most studied has been the nigrostriatal system. Dopaminergic cell bodies, located in the zona compacta of the substantia nigra, project primarily to the neostriatum and the amygdala. In the second system cell bodies of the mesolimbic dopamine system are located in the ventral tegmental area and innervate the nucleous accumbens and the olfactory tubercles. In the third system, cell bodies primarily from the hypothalamus innervate the external layer of the median eminence. Thus, areas of the brain such as the corpus callosum, in which D3r expression in oligodendrocytes was demonstrated, are surrounded by areas rich in dopamine. Certainly, at the time when oligodendrocyte precursors are dividing and migrating within the brain, i.e., shortly after birth in the mouse, the extracellular concentration of dopamine (which has diffused out of, or is released from the nerve terminal) is relatively high compared with adult levels (Howard et al., 1997), although the reuptake mechanism for the catecholamines is not yet fully developed (Nomura et al., 1976). The high water content of the brain could result in widespread diffusion of dopamine, such that dopamine could act on cells such as oligodendrocyte precursors. As oligodendrocytes reach their fully differentiated state in culture, D3r expression appears to disappear, which would make the fully mature oligodendrocytes refractory to the actions of dopamine or some other D3r ligand, at least through this receptor.

There is accumulating evidence that some neurotransmitters may participate in the modeling of neuronal shape and the outgrowth of their neurites (for review, see Mattson, 1988). Todd (1992) has shown that activation of D2r with quinpirole induced an increase in the neurite length of primary cultures of cortical neurons. More recently, Swarzenski et al. (1994) demonstrated that not only D2r, but also D3r and D4r, may regulate the outgrowth of neuronal processes. Indeed, the modulation of a number of neurotransmitter receptors during early brain development can dramatically influence the cytoarchitecture of different populations of neurons (Chubakov et al., 1986; Hauser et al., 1987; Mattson et al., 1989; Blanton et al., 1990; Lo Turco et al., 1991). It is not unreasonable, then, that in a cell whose primary function is to elaborate myelin membrane, at least the dopamine system, might modulate the cytoarchitecture of the oligodendrocyte in terms of the amounts and timing of myelin formation.

Recently, we reported the presence of the D2r in a subset of mature interfascicular oligodendrocytes in the rat corpus callosum (Howard et al., 1998). This and the present study have shown that cells in the oligodendrocyte lineage are capable of expressing members of the D2 subfamily of receptors. Although this remains to be investigated further, we suggest that the D3 and D2 receptors are expressed at different stages in oligodendrocyte development and that this expression may be involved in regulating myelin formation in the lineage. We propose that the immature oligodendrocytes express D3r, and this may serve to regulate myelin formation by some, as yet unknown, mechanism. This expression normally disappears in mature myelinating oligodendrocytes, rendering the cells unable to respond to dopamine or a related endogenous ligand and therefore enabling the cells to complete their differentiation. In our previous study D2r was not detected in oligodendroglial cells at early stages of brain development associated with oligodendroglial progenitor appearance and proliferation. This suggests that the expression of D3r in cells of the oligodendrocyte lineage occurs after D3r expression.

In summary, we have presented evidence that the D3r is present in cells within the oligodendrocyte lineage and that it may play a role in either the differentiation of oligodendrocytes and/or subsequent membrane formation by these cells. This adds another important function for dopamine in the developing brain beyond its well known role as a neurotransmitter.

    FOOTNOTES

Received Jan. 27, 1998; revised April 16, 1998; accepted May 1, 1998.

This work was supported by Grant NS23022 from the National Institutes of Health and Grants RG2693 (A.T.C.) and PP0573 (S.G.H.) from the National Multiple Sclerosis Society.

Correspondence should be addressed to Anthony T. Campagnoni, Mental Retardation Research Center and Brain Research Institute, School of Medicine, University of California at Los Angeles, 760 Westwood Plaza, Los Angeles, CA 90024. 

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Top
Abstract
Introduction
Materials & Methods
Results
Discussion
References

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