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The Journal of Neuroscience, November 15, 1998, 18(22):9245-9255

Nonobligate Role of Early or Sustained Expression of Immediate-Early Gene Proteins c-Fos, c-Jun, and Zif/268 in Hippocampal Mossy Fiber Sprouting

Walter K. Nahm and Jeffrey L. Noebels

Developmental Neurogenetics Laboratory, Department of Neurology, and Division of Neuroscience, Baylor College of Medicine, Houston, Texas 77030

    ABSTRACT
Top
Abstract
Introduction
Materials & Methods
Results
Discussion
References

Axon sprouting in dentate granule cells is an important model of structural plasticity in the hippocampus. Although the process can be triggered by deafferentation, intense activation of glutamate receptors, and other convulsant stimuli, the specific molecular steps required to initiate and sustain mossy fiber (MF) reorganization are unknown. The cellular immediate early genes (IEGs) c-fos, c-jun, and zif/268 are major candidates for the initial steps of this plasticity, because they encode transcription factors that may trigger cascades of activity-dependent neuronal gene expression and are strongly induced in all experimental models of MF sprouting. The mutant mouse stargazer offers an important opportunity to test the specific role of IEGs, because it displays generalized nonconvulsive epilepsy and intense MF sprouting in the absence of regional cell injury. Here we report that stargazer mice show no detectable elevations in c-Fos, c-Jun, or Zif/268 immediate early gene proteins (IEGPs) before or during MF growth. Experimental results in stargazer, including (1) a strong IEGP response to kainate-induced convulsive seizures, (2) no IEGP response after prolongation of spike-wave synchronization, (3) no IEGP increase at the developmental onset of seizures or after prolonged seizure suppression, and (4) unaltered levels of the intracellular Ca2+-buffering proteins calbindin-D28k or parvalbumin, exclude the possibility that absence of an IEGP response in stargazer is either gene-linked or suppressed by known refractory mechanisms. These data demonstrate that increased levels of these IEGPs are not an obligatory step in MF-reactive sprouting and differentiate the early downstream molecular cascades of two major seizure types.

Key words: spike-wave epilepsy; transcription factor; axon sprouting; hippocampus; plasticity; cell death

    INTRODUCTION
Top
Abstract
Introduction
Materials & Methods
Results
Discussion
References

Axonal sprouting and neosynaptogenesis play functional roles in reorganization and repair throughout the mammalian CNS (Cotman et al., 1981; West and Dewey, 1984; Fitzgerald et al., 1990; Darian-Smith and Gilbert, 1994). These processes are associated with complex patterns of activity and injury-dependent gene expression (Gall and Isackson, 1989; Nedivi et al., 1993; Chen et al., 1995; Konopka et al., 1995). The products of these plasticity-related genes are thought to be responsible for axonal outgrowth, guidance, and establishment of functional synapses. It is widely proposed that immediate early genes (IEGs) encode the initial transcription factors that modulate the expression of these genes (Sheng and Greenberg, 1990, Morgan and Curran, 1991a).

In the dentate gyrus, a well defined pattern of axon sprouting from the granule cell-mossy fiber (MF) system into the inner molecular layer has been found in several models of hypersynchronous activation, including kainic acid (KA) (Tauck and Nadler, 1985), kindling (Cavazos et al., 1991), and pentylenetetrazole (PTZ) (Golarai et al., 1992). Several lines of evidence link IEG induction with this process. Elevation of the mRNAs for c-fos, c-jun, and zif/268 precedes that of other genes associated with MF sprouting. Recurrent sprouting and IEG induction occur in the same cells, with sprouting occurring several days after the induction of IEGs (Morgan and Curran, 1991b). Both c-fos expression and MF-sprouting induction have roughly equivalent dose thresholds for PTZ (Dragunow and Robertson, 1987; Morgan et al., 1987). Drugs that prevent kindling and the induction of c-fos block MF sprouting (Kiessling and Gass, 1993). Finally, kindling-induced sprouting is impaired in c-fos null mutant mice (Watanabe et al., 1996).

Although the process of MF synaptic reorganization is highly correlated with IEG induction, all MF sprouting models described so far are also associated with regional cellular injury, death, synaptic loss, and gliosis (Cavazos and Sutula, 1990; Houser et al., 1990; Wallace and Fredens, 1992). Because IEG induction is closely associated with cell death (Curran and Morgan, 1995; Kasof et al., 1995), the presence of seizure-related injury obscures the actual stimulus for IEG expression, and it remains unclear whether immediate early gene proteins (IEGPs) are necessary or sufficient for aberrant axonal growth.

An opportunity to dissociate the role of IEGPs in MF sprouting from seizure-related damage arose with the neurological mutant stargazer. This mutant exhibits a stereotyped pattern of brief (1-10 sec) generalized six per second spike-wave (SW) discharges in the neocortex, hippocampus, and thalamus (Noebels et al., 1990). These hypersynchronous SW discharges occur frequently (one or two per minute) and precede by several weeks the progressive appearance of MF axon sprouting. No evidence of hippocampal cell death, gliosis, or cellular injury at the onset of sprouting has been found (Qiao and Noebels, 1993; Chafetz et al., 1995). To determine whether the hippocampal IEGP expression pattern is coupled to MF sprouting despite the absence of cellular injury, we compared the neuronal staining patterns of polyclonal antibodies to c-Fos, c-Jun, and Zif/268 in stargazer with those expressed in a sprouting model induced by KA. We also examined relative IEGP activation thresholds, the potential involvement of refractory mechanisms inhibiting IEGP induction, and the role of two Ca2+-binding proteins, calbindin-D28k and parvalbumin.

    MATERIALS AND METHODS
Top
Abstract
Introduction
Materials & Methods
Results
Discussion
References

Mice. Homozygous stargazer (C3B6Fe+ stg/stg) and wild-type (C57Bl/6J +/+) mice were obtained from breeding colonies of The Jackson Laboratory (Bar Harbor, ME) and Baylor College of Medicine. Animals were housed under a 12 hr light/dark cycle with ad libitum access to food and water. Unless otherwise noted, mice used in experiments were adults ranging from 2-6 months old.

Electrocorticographic recordings. Mice were anesthetized with Avertin (1.25% tribromoethanol/amyl alcohol) by intraperitoneal injection (0.02 ml/gm body weight). Teflon-coated silver wire electrodes (0.005 inch diameter) soldered to a microminiature connector were implanted through burr holes (0.1 inch diameter) of the skull into the subdural space over the frontal and occipital cortices (Noebels et al., 1990). The microminiature connector was affixed permanently to the midline surface of the skull with cyanoacrylate glue and dental cement. All experiments and electrocorticographic (ECoG) recordings commenced 1 week after surgery. ECoG activity in alert and unrestrained mice was recorded using a Grass model 6 electroencephalograph.

Convulsive seizure induction. Wild-type and stg/stg mice were injected with KA (30 mg/kg, i.p.), a convulsant known to induce seizures that precede MF synaptic reorganization in the hippocampus (Tauck and Nadler, 1985). By the first 20-30 min after injection, both genotypes exhibited typical class V limbic seizures (Racine, 1972) consisting of episodes of motionless behavior, followed by myoclonic facial movements, forepaw tremor, rearing, and falling with loss of postural balance. In both stg/stg and +/+, status epilepticus occurred 1.5-2 hr after injection, followed by generalized tonic-clonic convulsions. Stargazer mice displayed on average more severe seizures and a quicker onset to seizures than did wild-type mice. Mice were killed for immunohistochemistry (IHC) 2 hr after KA injection to assess regional c-Fos, c-Jun, and Zif/268 expression in the brain.

(±)-3-(2-carboxypiperazin-4-yl)-propyl-1-phosphonic acid treatment. Pilot experiments revealed that stg/stg mice injected intraperitoneally with 40 µM/kg of the competitive NMDA receptor antagonist (±)-3-(2-carboxypiperazin-4-yl)-propyl-1-phosphonic acid (CPP) displayed prolonged episodes of continuous SW ECoG activity lasting 60 min or longer (Nahm and Noebels, 1995). The proepileptic effect of this treatment, which had no discernable effect on ECoG activity in +/+ mice and blocked SW activity in another SW-MF sprouting model, tottering (Stanfield, 1989), was used to experimentally enhance SW synchronization in stg/stg. One hour of ECoG recordings was first obtained in stg/stg mutants to quantify baseline SW activity. Within thirty min of CPP injection, near-continuous SW synchronization appeared (individual discharges increased 10- to 100-fold in duration). These animals were killed 2 hr after the onset of prolonged SW activity to examine regional IEGP expression in the brain.

Ethosuximide treatment. To produce a complete blockade of SW seizure activity for a period of 30 hr, an stg/stg mouse was injected intraperitoneally with 200 mg/kg of ethosuximide (ETX) (Heller et al., 1983; Marescaux et al., 1992) approximately every 2 hr. Continuous ECoG monitoring was performed to determine dose intervals and to verify the absence of SW discharges. At the end of the seizure-free period, ETX injections were discontinued, and the mouse was killed for IHC 2 hr after the spontaneous SW seizure activity resumed.

Immunohistochemistry. Mice were deeply anesthetized with Avertin (0.02 ml/gm, i.p.) and perfused transcardially with 0.1 M phosphate buffer (PB), pH 7.4, followed by 4% paraformaldehyde in 0.1 M PB. The brains were removed, post-fixed for 24 hr, and immersed in 30% sucrose in 0.1 M PB for 5 d. Horizontal sections (40 µm thickness) through the whole brain were cut on a freezing microtome and pretreated in 0.03% H2O2 in absolute methanol. Tissue sections were then immersed in 2% bovine serum albumin (BSA) in 0.1 M PBS for 3 hr and processed using the free-floating method with thorough rinsing between steps. Sections were incubated at 4°C with either: (1) 1:400 c-Fos sheep polyclonal antibody (Cambridge Technologies, Watertown, MA) in 0.1 M PBS with 1% BSA for 24 hr; (2) 1:400 c-Jun rabbit polyclonal antibody (Oncogene Science) in 0.1 M PBS with 1% BSA for 24 hr; (3) 1:300 Zif/268 rabbit polyclonal antibody (Santa Cruz Biotechnology, Santa Cruz, CA) in 0.1 M PBS with 1% BSA for 48 hr; (4) 1:200 calbindin-D28k mouse monoclonal antibody (Sigma, St. Louis, MO) in 0.1 M PBS with 1% BSA for 24 hr; or (5) 1:400 parvalbumin mouse monoclonal antibody (Sigma) in 0.1 M PBS with 1% BSA for 24 hr. Sections were then incubated for 60 min at room temperature with the appropriate biotinylated secondary antibody (Jackson ImmunoResearch, West Grove, PA) at 1:100, and followed by an incubation in an avidin-biotin-horseradish peroxidase (HRP) complex (ABC kit; Vector Laboratories, Burlingame, CA) (Hsu et al., 1981). The bound peroxidase was localized by incubating sections in 0.1% 3,3'-diaminobenzidine (DAB) and 0.025% H2O2 at room temperature for 5-10 min, which generated the visible substrate. Sections incubated without the primary antibody served as controls. All the sections were examined and photomicrographed using standard light microscopy (Leitz, Orthoplan 2 and Vario Orthomat 2). Because variation occurs along the septal-temporal gradient, all levels of the hippocampal formation were analyzed in all experiments. A 0-4+ rating scale was used to quantify visible substrate staining intensity in cell nuclei using standard light microscopy. All slides were scored by a single observer to ensure consistency. A score of 0 was assigned when staining was completely absent. A score of 1+ was assigned to minimal basal level staining, 2+ for moderate staining, and 3+ for high staining. The greatest score of 4+ was only assigned to maximal nuclear staining patterns usually observed in the dentate gyrus under KA administration.

    RESULTS
Top
Abstract
Introduction
Materials & Methods
Results
Discussion
References

Absence of elevated IEGP expression in stg/stg hippocampus

Regional expression patterns of c-Fos, c-Jun, and Zif/268 were assayed in 12 adult mutant mice (stg/stg) and compared with those of 12 age-matched wild-type control (+/+) mice. All stg/stg and +/+ mice displayed a consistent lack of nuclear c-Fos staining in the dentate granule cells and other neurons of the hippocampus (Fig. 1). In both genotypes, minimal basal levels of specific nuclear c-Fos staining were found sporadically in the neocortex, thalamus, and septum. Specific staining throughout the remainder of the mutant brain was negligible, and no significant genotypic differences in regional c-Fos nuclear staining was identified (Table 1).


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  		Figure 1.   Stargazer mutants show no resting elevations in c-Fos, c-Jun, or Zif/268. Comparison of sprouting pattern, electrocortical activity, and IEG protein expression patterns in the hippocampus of adult stg/stg, +/+, and +/+-KA mice (2 hr after kainic acid-induced limbic seizures). Top, Stargazer and +/+-KA mice display strong mossy fiber sprouting in the inner molecular layer of dentate gyrus. Representative 8 sec samples of ECoG recordings display spontaneous six or seven per second spike-wave discharges in the mutant that are not present in the wild-type control, whereas the ECoG recordings during kainic acid convulsive seizures show continuous discharges associated with tonic neuronal depolarizations. Bottom, In temporal hippocampus, stg/stg mice are devoid of c-Fos and Zif/268 staining in a pattern identical to +/+ mice and show moderate antibody staining to c-Jun in dentate granule cells and the pyramidal cell layers. Wild-type mice with KA-induced seizures display a strong c-Fos, c-Jun, and Zif/268 response that is greatest in the DG. Moderate elevations of c-Fos and Zif/268 and lesser elevations of c-Jun were seen in CA1, CA2, and CA3 pyramidal regions. Scale bar, 100 µm.

                              
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  		Table 1.   Regional IEG protein antibody stain intensity in stg/stg, +/+, and +/+-KA hippocampus

In contrast to SW seizures found in the mutant, limbic seizures induced by KA produced clear increases in nuclear c-Fos expression in six assayed adult wild-type (+/+) mice. c-Fos staining in the KA model (+/+-KA) was most intense in the dentate granule cells, and elevated in pyramidal neurons of all the hippocampal subregions (CA1, CA2, and CA3) (Fig. 1). c-Fos expression was also diffusely elevated in neuronal nuclei of the entorhinal cortex, thalamus, basal ganglia, septum, and most layers of the neocortex (Table 1).

The pattern of polyclonal antibody staining to c-Jun revealed moderate immunoreactivity in all hippocampal subregions of both stg/stg and +/+ brains (Fig. 1). As in the c-Fos experiments, both genotypes displayed light or negligible c-Jun staining in the neocortex and thalamus, and no consistent differences in the density of neuronal staining for c-Jun were observed between the two genotypes. KA-induced seizures produced large increases in c-Jun staining in the wild-type dentate gyrus and CA1 pyramidal neurons and elevated to a lesser degree c-Jun staining in the hippocampal CA3 subregion. However, no consistent c-Jun staining above basal levels was found after a KA seizure in any other brain area (Table 1).

Both stg/stg and +/+ displayed identical patterns of Zif/268 antibody staining in the forebrain. In the septal hippocampus of both genotypes, there was light staining in the dentate gyrus and CA1 pyramidal cells. The temporal hippocampus, however, was devoid of Zif/268 staining in both genotypes. In stg/stg there was moderate Zif/268 staining in neocortex, entorhinal cortex, and basal ganglia; this pattern of staining was identical to that found in +/+ mice. The pattern of Zif/268 expression in +/+ mice treated with KA showed extreme elevations in dentate granule cells and CA1 pyramidal neurons (Fig. 1; Table 1). Zif/268 expression was slightly elevated in CA2 but conspicuously absent in CA3 neurons. KA-induced seizures also greatly raised the number of Zif/268-stained neurons in the neocortex and entorhinal cortex, and diffuse, moderate elevations of Zif/268 staining were found in the thalamus, basal ganglia, and septum (Table 1). These experiments demonstrate the presence of a striking difference in hippocampal IEGP expression between two types of hypersynchronous seizure activity that induce similar patterns of MF sprouting (spike-wave seizures in stg/stg and convulsive seizures in +/+-KA).

To ensure that the absence of an IEGP response to SW discharge activity was not specific for the stargazer mutation, we analyzed an adult tottering mouse, a mutation on a different genetic background that also shows SW discharge and sprouting activity (Stanfield, 1989). No abnormal c-Fos staining was observed, and c-Fos elevation was not seen in the neocortex of an inbred rat strain with SW discharges (Willoughby et al., 1993), suggesting that the SW pattern itself may be ineffective in inducing IEGP activity regardless of genetic background.

IEGP induction patterns in stargazer brain

The absence of IEGP induction above basal levels in stg/stg hippocampus, thalamus, and neocortex was unexpected, considering the frequent bursts of SW synchronization generated in these areas and the strong increase in IEGP levels that follow neuronal activation during synchronous discharges in other seizure models. This finding raised the possibility that the SW pattern might synchronize neurons by activating synaptic networks below the depolarization threshold required to initiate a measurable IEGP response, suggesting that the intensity of this pattern of abnormal synchronization differed significantly from those found in other experimental epilepsy models. Other factors, however, could potentially mask the induction of a detectable c-Fos, c-Jun, and Zif/268 response in the mutant and mitigate this conclusion, namely: (1) a gene-linked defect in one or more steps of the stimulus transduction pathway of IEGP expression in stg/stg neurons; (2) the brevity (1-10 sec) of individual SW episodes relative to seizures in other experimental models; (3) short-term inactivation of IEGP expression in cells engaged in SW bursting; (4) longer-term cellular refractory mechanisms for sustained IEGP expression activated by chronic SW stimulation; and (5) increased buffering capacity of intracellular free Ca2+ in stg/stg neurons. These possibilities were evaluated in a series of control experiments (Figs. 2-6).

Robust stimulus-induced IEGP expression in stg/stg neurons

To test whether the lack of IEGP induction in stg/stg neurons during SW activity might be caused by a gene-linked neuronal defect in the intervening steps of the transynaptic induction pathway, six stg/stg mice were injected with KA 30 mg/kg intraperitoneally (stg/stg-KA), monitored for ECoG seizure activity for 2 hr, and killed for IHC (Fig. 2A). Within the first 15 min after injection, mutant mice exhibited typical class I-II seizure activity. This activity was soon followed by class V limbic seizures and generalized tonic-clonic seizures. In a similar manner to wild-type mice, the seizures induced by KA produced clear increases in nuclear expression of the IEGPs, c-Fos, c-Jun, and Zif/268, in stg/stg. In the stg/stg hippocampal formation, KA produced increases in expression of c-Fos in nuclei of DG, CA1, CA2, and CA3 neurons (Fig. 2B). In addition, strong c-Fos staining was observed in neocortex, entorhinal cortex, thalamus, basal ganglia, and septum. Clear increases in c-Jun expression were found in the DG, and smaller elevations were found in CA1, CA2, and CA3 regions (Fig. 2B). No c-Jun staining above basal levels was found in any other brain area. KA-induced seizures produced a pattern of intense Zif/268 elevation in DG neurons and moderate elevations of Zif/268 in the hippocampal pyramidal layer subregions (CA1, CA2, and CA3) in stg/stg (Fig. 2B). The mutant neocortex and entorhinal cortex also displayed a strong elevation of Zif/268, whereas the thalamus, basal ganglia, and septum showed mild Zif/268 increases. These data show that stg/stg neurons are capable of seizure-induced IEGP expression. More precisely, these data demonstrate that the specific population of dentate granule cells in the temporal hippocampus of stg/stg that are involved in recurrent MF sprouting can display a robust induction of c-Fos, c-Jun, and Zif/268.


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  		Figure 2.   Stargazer mice demonstrate a strong IEGP response after KA-induced seizures. A, ECoG recordings demonstrate that inherited spike-wave seizures were converted to tonic discharges after intraperitoneal administration of 30 mg/kg of KA. B, IEGP expression patterns in stg/stg examined 2 hr after KA treatment reveal strong inductions of c-Fos and Zif/268 in DG, CA1, CA2, and CA3. Strong c-Jun expression was found in the DG, and lesser elevations of c-Jun were detected in the pyramidal layers. Scale bar, 100 µm.

Prolongation of SW activity does not increase IEGP expression

Preliminary pharmacological survey revealed that the NMDA receptor glutamate-binding site antagonist CPP paradoxically increases SW seizure activity in stargazer, in contrast to its antiepileptic effects in other epilepsy models. To test the possibility that spontaneous SW seizures in stg/stg were simply too brief (1-10 sec) or infrequent (one or two per minute) to elevate IEGPs over basal levels, IHC to c-Fos, c-Jun, and Zif/268 was performed on seven stg/stg mice injected (40 µg/kg, i.p.) with CPP (stg/stg-CPP). Within 30 min of treatment, the ECoG of these mice converted from a pattern of brief intermittent SW discharges to a nearly continuous (95% of elapsed time) pattern of typical six per second SW bursting lasting ~2 hr accompanied by behavioral immobility (Fig. 3A). Although the possibility exists that CPP, an NMDA receptor antagonist, might indirectly block IEGP induction, recent evidence shows that other NMDA antagonists MK-801 (Dragunow and Faull, 1990; Sharp et al., 1990; Hughes et al., 1993) and ketamine (Nakao et al., 1993) allow distinct induction of IEGPs in the CNS, presumably through non-NMDA receptor Ca2+ signaling pathways (Lerea et al., 1992).


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  		Figure 3.   Prolongation of SW activity with CPP does not induce c-Fos expression pattern in stg/stg. A, ECoG recordings in a stg/stg mutant demonstrate the conversion of spontaneous SW discharges to prolonged status-like SW activity after injection of CPP (40 µM/kg, i.p.). B, After 2 hr of CPP-induced prolonged SW activity, stg/stg hippocampus displays no change in c-Fos expression. c-Jun and Zif/268 levels were also unchanged (data not shown). Scale bar, 100 µm.

Sections from CPP-treated stg/stg mice killed after 2 hr of continuous SW activity showed a pattern of c-Fos, c-Jun, and Zif/268 staining in the hippocampal formation identical to that seen in untreated stg/stg mice (Fig. 3B). No increases in c-Fos over basal levels were detected throughout all other brain regions examined, and similar results were found with c-Jun and Zif/268 staining patterns. The low levels of hippocampal IEGP expression were identical to the basal levels seen in untreated stg/stg mice (Fig. 1). These data suggest that the brief duration of individual spontaneous SW discharges is not a critical factor in the lack of a sustained IEGP response in the mutant, and that the amount of neuronal depolarization during this pattern of activity remains below the threshold for IEGP induction.

Short-term refractory mechanisms are not responsible for the lack of IEGP induction in stg/stg mice

Seizures induced by an initial dose of the convulsant PTZ are followed by a short (up to 16 hr) period during which a second dose of PTZ is unable to further induce c-fos (Morgan et al., 1987). A desensitization of this type to c-fos and zif/268 induction in the brain is also found after acute administration of cocaine (Ennulat et al., 1994). This interval in which IEGs are persistently suppressed even in the presence of an effective stimulus is referred to as a short-term refractory period. To determine whether short-term refractoriness may have contributed to the lack of IEGP response in stg/stg mice, SW seizures were completely blocked for 30 hr with repeated injections of ETX (Fig. 4A). At the end of this time, ETX administration was discontinued, and seizure activity was allowed to return for 2 hr. The stg/stg mouse brain was then examined for IEGP expression. No changes in c-Fos expression in granule cells or other brain regions were found after the ETX treatment in comparison to untreated stg/stg mice (Fig. 4B). Similarly, there were no apparent changes in c-Jun or Zif/268 expression patterns. These data imply that short-term refractory mechanisms do not contribute to the lack of stg/stg IEGP induction during SW seizures.


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  		Figure 4.   Short-term refractory mechanisms do not account for lack of c-Fos expression in stg/stg mice with chronic SW seizures. A, Schematic diagram shows blockade of basal SW discharge activity in a mutant mouse for 30 hr with ethosuximide injections. SW seizures (approximately one per minute) were allowed to resume, and IHC for IEG proteins was performed 2 hr later. B, IHC of stg/stg hippocampus after seizure suppression treatment shown in A reveals no abnormal induction of c-Fos. c-Jun and Zif/268 patterns were also unchanged (data not shown). Scale bar, 100 µm.

Postnatal day 17 and 18 stg/stg mutants do not display IEGP elevations over basal levels

Winston et al. (1990) reported a second, longer lasting (>1 day) refractory period for c-fos and c-jun mRNA induction after chronic electroconvulsive seizures. Unlike short-term inactivation, this inhibition was maximal after 8-10 d of daily stimulation and did not appear to be related to c-Fos autoregulation, because it occurred in the absence of increased c-Fos protein. To test whether similar long-term refractory mechanisms maintained by chronic SW seizures might result in a persistent block of IEGP induction, stg/stg brains were examined within 24 hr of the developmental onset of SW seizures during the third postnatal week, postnatal days 17 and 18 (P17-P18) (Fig. 5A) (Qiao and Noebels, 1993). Because the capacity for c-fos induction is already well established at this age (Schreiber et al., 1992; Jensen et al., 1993; Smeyne et al., 1993), the potential for IEGP induction by SW seizure activity in P17-P18 stg/stg mice should not be masked by either short- or long-term IEGP expression refractory mechanisms.

As in the adult, hippocampal sections from P17-P18 stg/stg displayed no evidence of increased c-Fos staining relative to the wild type (Fig. 5B). Similar results were found with c-Jun and Zif/268 antibodies. In other brain regions, the only significant findings among the three IEGP expression patterns were slight elevations in Zif/268 staining present diffusely in neocortex, entorhinal cortex, and basal ganglia in the young mutants compared with the adults.


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  		Figure 5.   Long-term refractory mechanisms do not account for absence of c-Fos induction in stargazer mice. A, Schematic diagram illustrates the experimental time point for IEGP analysis, performed within 1 d of the developmental onset (P17-P18) of spike-wave seizures in stg/stg mutants. B, IHC of P17-P18 stg/stg hippocampus reveals the absence of c-Fos expression after spike-wave seizure onset. No c-Jun or Zif/268 changes in this tissue were seen (data not shown). Scale bar, 100 µm.

These data indicate that neither type of refractory mechanism is likely to account for the lack of c-Fos, c-Jun, and Zif/268 induction in stargazer mutants. The previous finding that KA-induced seizures in adult stg/stg caused marked c-Fos, c-Jun, and Zif/268 induction also supports this conclusion. If refractory mechanisms had been recruited by chronic SW activity, the IEGP response after KA administration in stg/stg mice would have been diminished relative to that in KA-treated +/+ mice.

stg/stg neurons show no increases in the Ca2+-buffering proteins calbindin-D28k and parvalbumin

Several studies show that increases in intracellular Ca2+ are required for depolarization or seizure-induced expression of IEGs (Morgan and Curran, 1986; Lerea and McNamara, 1993). Intracellular calcium may be modulated in cells by the EF-hand (Persechini et al., 1989) calcium-binding proteins (CaBPs), such as calbindin-D28k and parvalbumin (Morrissey et al., 1978; Baimbridge et al., 1982; Celio, 1986; Kohr et al., 1991). The levels of these proteins within the hippocampal formation correlate with vulnerability to excitotoxic induced cell death or neurodegeneration (Sloviter, 1989; Hof et al., 1991; Mattson et al., 1991; Sloviter et al., 1991) and can be changed by activity-dependent processes, including seizures (Miller and Baimbridge, 1983; Kamphuis et al., 1989; Lowenstein et al., 1991).

To determine whether SW seizures might regulate the level of cytoplasmic CaBPs, with potential secondary effects on transynaptic IEGP expression, the patterns of calbindin-D28k and parvalbumin IHC in stg/stg brains were compared with those in +/+ mice. Adult stg/stg mutants displayed normal hippocampal patterns of calbindin-D28k and parvalbumin expression relative to the +/+ mice (Fig. 6). In both genotypes, calbindin-D28k was expressed in DG and CA1, but not CA3 pyramidal cells. The dentate granule cell MF projections to CA3, and the dendritic layer (stratum oriens) of CA1 showed marked calbindin-D28k expression. No genotypic differences were observed in other brain regions. Parvalbumin expression differed from the pattern seen with calbindin-D28k antibody. Parvalbumin was expressed in a fiber plexus surrounding dentate granule cells and hippocampal pyramidal cells. Most parvalbumin staining was found in interneurons of the DG (stratum granulosum) and pyramidal cell layer (stratum pyramidale), but was also not altered in the mutant hippocampus. In both genotypes, scattered parvalbumin staining neurons were found in the neocortex, thalamic relay nuclei, and the thalamic reticular nucleus. In addition, strong staining was observed in the cerebellar cortex, particularly in the Purkinje cell layer. No genotypic differences in the pattern of parvalbumin expression were observed. These data provide no evidence for the seizure-induced changes in calbindin-D28k or parvalbumin seen in convulsive seizure models that could alter intracellular Ca2+ buffering or IEGP induction.


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  		Figure 6.   Inherited spike-wave seizures do not alter calcium-binding protein levels in the stargazer hippocampus. Comparison of calbindin-D28k and parvalbumin immunohistochemistry in adult stg/stg and its wild-type control. No consistent differences in the staining patterns of these two calcium-binding proteins were detected between these two genotypes. Scale bar, 100 µm.

    DISCUSSION
Top
Abstract
Introduction
Materials & Methods
Results
Discussion
References

The absence of elevated IEGP expression in stg/stg hippocampus before and during the period of MF sprouting demonstrates that specific increases in c-Fos, c-Jun, and Zif/268 seen in convulsive models of epilepsy are not obligatory signals for hippocampal MF reorganization. The failure of SW synchronization to elicit cellular IEGP responses in the stargazer absence epilepsy model is apparently not prevented by stimulus-transcription coupling defects in hippocampal neurons, subthreshold seizure activation, intracellular refractory mechanisms, or abnormal levels of the Ca2+-buffering proteins calbindin-D28k and parvalbumin. These findings demonstrate that alternative gene transcription pathways for triggering MF sprouting must exist. In addition, because the synchronization patterns of SW and tonic-clonic seizures differentially regulate IEGP expression, our data suggest that these two seizure types are associated with marked differences in neuronal depolarization and calcium influx and give rise to fundamentally distinct patterns of downstream molecular signaling.

c-Fos, c-Jun, and Zif/268 increases are not obligatory for MF reorganization

Elevations of IEG expression correlate well with pathological sprouting of nerve axons at several sites in the nervous system (Bao et al., 1993; Herdegen et al., 1993; Kenney and Kocsis, 1998), and the induction of c-fos, c-jun, and zif/268 have been specifically correlated with MF sprouting (Sutula et al., 1988; Popovici et al., 1990; Simonato, 1993; Kiessling and Gass, 1993; Watanabe et al., 1996; Represa and Ben-Ari, 1997). Although these models are accompanied by early or delayed cell death, stargazer mutants display an identical pattern of MF recurrent sprouting with little evidence of neuronal cell loss except at very late stages (Qiao and Noebels, 1993). The presence of sprouting in the absence of elevations of c-Fos, c-Jun, and Zif/268 in the mutant demonstrate that these IEGPs are not obligatory in hippocampal synaptic reorganization and that other regulators of downstream neuronal gene expression induce this pattern of reactive axonal plasticity.

In a test of this hypothesis, Watanabe et al. (1996) reported that a null mutant of c-fos showed impaired development of kindling and reduced MF sprouting and concluded that there was a functional role for c-fos in triggering new axon collateral growth in granule cells. However, these authors could not exclude maturational alterations in the c-fos -/- mutant brain preceding the kindling experiment that might diminish both processes, and the specific contribution of c-Fos in activating neuronal growth-related genes after seizures in the limbic system remained untested. Some kindling-induced sprouting was still observed in c-fos -/- mice, and it was suggested that alternative transcription factor pathways might compensate for the absence of c-fos. In contrast to the c-fos null mutation, a related study of zif/268 (NGFI-A) null mutants concluded that zif/268 did not play a required role in the expression of MF sprouting (Zheng et al., 1998). In the stargazer model, unlike the null mutations, the IEGPs c-Fos, c-Jun, and Zif/268 are available throughout brain development. Our findings specifically demonstrate that they are not induced before or during sprouting and, therefore, play a nonobligate role.

Along with our evidence that they are not necessary for reactive sprouting, c-Fos protein increases are also not sufficient to initiate reactive plasticity, because not all models with pathological c-Fos expression in granule cells show MF sprouting. Although KA induction uniformly produced elevated granule cell c-Fos elevations (Le Gal La Salle, 1988; Popovici et al., 1988; Sonnenberg et al., 1989; Pennypacker et al., 1993), Cronin et al. (1992) has shown that of 25 rats with KA-induced seizures only 17 displayed the dense MF sprouting response.

The elevation of c-Fos, c-Jun, Zif/268, and other IEGPs [e.g., JunD and the 35-37 kDa fos-related antigen (FRA)] (Bing et al., 1996, 1997; Feng et al., 1997) has been viewed as an attractive mechanism for inducing axonal growth because they are transcription factors that bind to activator protein-1 (AP-1) sites. AP-1 is a promoter element regulating a variety of genes reported to contribute to sprouting, such as growth-associated protein-43 (GAP-43) (Eggen et al., 1994; Bendotti et al., 1994, 1997) and nerve growth factor (NGF) (Adams et al., 1997). Murine overexpression of a GAP-43 transgene potentiates MF reorganization (Aigner et al., 1995), and null mutants of GAP-43 display axon pathfinding defects (Strittmater et al., 1995). Induction of neurotrophic factors like NGF, neurotrophin-3, brain-derived neurotrophic factor, and basic fibroblast growth factor (bFGF) have been associated with sprouting (Gall, 1993; Gall et al., 1994; Kar et al., 1997; Mathern et al., 1997), and blockade of the tyrosine kinase B receptor for growth factors abolishes induction of MF sprouting (Hughes et al., 1998). Anti-NGF IgGs and NGF domains that interfere with neurotrophin binding also inhibit kindling-induced sprouting (Rashid et al., 1995; Van der Zee et al., 1995), and the time course of increased neurotrophic activity after KA administration parallels MF reorganization (Lowenstein et al., 1993). Finally, other mechanisms regulating sprouting have been proposed that are not directly related to AP-1 activation in neurons. Represa and Ben-Ari (1997) suggest that MF terminal branches may sprout under the influence of local astrocytes that could favor axon growth by secreting neuronal cell adhesion molecule (Niquet et al., 1993) and trophic factors such as bFGF or by not secreting inhibitory substrate molecules such as tenascin-C (Niquet et al., 1995).

Convulsive and nonconvulsive synchronization give rise to different patterns of stimulus-transcription coupling

The finding that convulsive and SW synchronization display differential patterns of IEGP expression suggests that these two seizure types may produce distinct profiles of AP-1-binding complexes in the CNS. Because specific temporal patterns of AP-1-induced genes can result in altered long-term plasticity, this pathway for gene regulation may contribute to the variations in neurite outgrowth, neuronal differentiation, and cell death found between convulsive and SW seizures.

Several lines of evidence indicate that prolonged IEGP elevations may be more closely related to cell death than seizure-induced neurite outgrowth; this hypothesis is compatible with the differences observed in IEGP expression between the models of convulsive and nonconvulsive epilepsy. c-fos expression can be an indicator of impending death in certain neurons in the developing and adult CNS (Gonzalez-Martin et al., 1992; Smeyne et al., 1993; Dragunow and Preston, 1995; Kasof et al., 1995; Goodenough et al., 1997). IEGP elevations are more prolonged after KA seizures, in which there is substantial cell death, than after PTZ seizures, in which there is not (Kasof et al., 1995). c-Jun is associated with cell death in the hippocampus after status epilepticus and ischemia (Dragunow et al., 1993). Certain classes of neurons have large increases in c-jun and undergo apoptosis when deprived of NGF. Ham et al. (1995) demonstrated that a c-Jun dominant negative mutation protected mouse sympathetic neurons from apoptotic cell death induced by NGF withdrawal, and showed that overexpression of c-Jun in sympathetic neurons induced apoptosis. KA-induced convulsive seizures are followed by marked regional induction of IEGPs in specific hippocampal pyramidal neurons, and these areas show the most severe loss of cells (Nadler et al., 1980a,b; Nitecka et al., 1984). Conversely, SW seizures in the stargazer model are associated with only a minor and late loss of hilar interneurons in the mutant hippocampus not seen until several months after the onset of the seizures (Qiao and Noebels, 1993).

Alteration in IEGP expression between spike-wave and convulsive seizure patterns may depend on different levels of depolarization and calcium entry

Why don't hypersynchronous spike-wave absence seizures induce IEGP expression? Since the discovery that c-fos mRNA transcripts rapidly rise after depolarization and influx of calcium in PC12 cells (Greenberg et al., 1986; Morgan and Curran, 1986), calcium has been linked as a required second messenger for c-fos induction (Morgan and Curran, 1991a), along with the activation of other regulatory elements (Robertson et al., 1995). Although the route by which calcium enters a neuron during a seizure may differ, the resulting transcription of c-fos still occurs (Lerea et al., 1992), and calcium entry during different patterns of stimulation may lead to distinct arrays of IEG expression. The fact that SW seizures in stg/stg do not induce IEGPs, whereas KA-induced seizures produce robust responses, and the fact that stg/stg and +/+ mice show equivalent levels and patterns of two CaBPs suggests that SW seizures cause (1) lower depolarizing levels, and (2) lesser degrees of Ca2+ influx in synchronized neurons than do convulsive seizures.

This tentative conclusion is supported by proposals (Gloor, 1979) that the SW discharge is a true "inhibitory" seizure, a pattern of generalized synchronous oscillations in neurons deficient in paroxysmal depolarization shifts (PDSs) and showing increased postsynaptic inhibition, as opposed to the seizures characteristic of convulsive epileptic syndromes. According to this hypothesis, the repatterning of cellular excitation during the brief "spike" of a SW seizure is not caused by excessive neuronal depolarization, and impulse activity does not exceed the normal range; the prolonged IPSPs (>250 msec) corresponding to the inhibitory ECoG "wave" also predict substantially less neuronal depolarization throughout the duration of the seizure episode (Gloor, 1984; Giaretta et al., 1987). This proposed mechanism to account for the lack of IEGP expression during SW seizures has been generally confirmed during in vivo neocortical intracellular recordings in the SW epileptic rat that show only brief depolarizations without PDSs during SW seizures, and no decreases in extracellular Ca2+ levels determined by Ca2+-sensitive microelectrodes as seen during convulsive discharges (Heinemann et al., 1977; R. Pumain, personal communication). Initial in vivo studies in a rat SW model using two photon confocal microscopy also confirm the absence of increased intracellular free Ca2+ during SW patterns of synchronization (Buzsaki et al., 1997).

    FOOTNOTES

Received July 15, 1998; revised Aug. 26, 1998; accepted Sept. 1, 1998.

This work was supported by National Institutes of Health Grant NS29709 and The Blue Bird Circle Foundation for Pediatric Neurology.

Correspondence should be addressed to Dr. Jeffrey L. Noebels, Department of Neurology, Baylor College of Medicine, One Baylor Plaza, Houston, TX 77030.

    REFERENCES
Top
Abstract
Introduction
Materials & Methods
Results
Discussion
References

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