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Figure 3.
Expression of
eat-4:: reporters. The reporter constructs
schematically shown in Figure 1A were used to
generate transgenic animals. A, Expression of
eat-4:: lacZ(nls) in the
pharynx, observed with Nomarski optics. 
-Galactosidase activity was
found in the nuclei of two types (each consists of a bilaterally
symmetric pair) of pharyngeal neurons, M3 and
NSM. Also seen in this panel is the strong signal in the
nucleus of an extrapharyngeal cell posterior to M3. This
extrapharyngeal cell is probably a neuron in the IL1 class.
B, C, Expression of
eat-4:: gfp in extrapharyngeal neurons. The
identity of these cells is marked either in the figure
(B) or above the photograph
(C). The identity of one cell (marked as
AVJ or AIN) is uncertain. In
B, the entire animal is shown, whereas in
C only the head is shown. Only the right half of the
animal is shown in C, because these classes of cells are
bilaterally symmetric. C, Shown is an overlay (with
Adobe Photoshop software) of nine serial confocal images with an
intersection spacing of 0.9 µm.
