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Previous Article | Next Article
The Journal of Neuroscience, September 15, 2000, 20(18):6927-6938
Local Permutations in the Glomerular Array of the Mouse Olfactory Bulb
Jörg Strotmann1, 2, Sidonie Conzelmann2, Anja Beck2, Paul Feinstein1, Heinz Breer2, and Peter Mombaerts1 1 The Rockefeller University, New York, New York 10021, and 2 Institute of Physiology, University Stuttgart-Hohenheim, D-70593 Stuttgart, Germany
ABSTRACT
TOP
ABSTRACT
INTRODUCTION
Olfactory sensory neurons expressing a given odorant receptor gene project their axons with great precision to a few specific glomeruli in the olfactory bulb. It is not clear to which extent the positions of these glomeruli are fixed. We sought to evaluate the constancy of the glomerular array in the mouse by determining the relative positions of glomeruli for various odorant receptors, using a method that affords single-axon resolution, and in a large number of bulbs. We used a genetic strategy to visualize neuronal populations that express one of three members of the mOR37 subfamily. We generated by gene targeting five strains of mice in which expression of a given mOR37 gene is linked to expression of an axonal maker, which is either taulacZ or tauGFP. The patterns of marker expression faithfully mimic those of the cognate receptors. Axons of neurons expressing a given mOR37 gene converge onto one or two glomeruli per bulb. Each mOR37 gene has its own glomeruli, and the mOR37 glomeruli are grouped within a restricted domain of the bulb. Serial sectioning of 214 bulbs reveals that the relative positions of the three types of glomeruli are not fixed but display local permutations. Importantly, this is also the case among the two bulbs from one individual, ruling out the genetic manipulation itself and differences in genetic background or olfactory experience as causes for the observed variability. These local permutations may reflect the developmental history of the glomeruli and are relevant for the construction of spatial odor maps.
Key words: olfaction; olfactory system; olfactory bulb; glomerulus; sensory neuron; olfactory receptor; odorant receptor
INTRODUCTION
The vertebrate olfactory system detects and discriminates numerous chemical compounds. Odorant reception initiates when odorous molecules interact with specific odorant receptors (ORs) on the surface of olfactory sensory neurons (OSNs) (Shepherd, 1994
). The OR repertoire is encoded by the largest gene family in the vertebrate genome, comprising as many as 1000 genes in mouse and rat (Buck and Axel, 1991
-galactosidase staining of the cell bodies, axons, and axon terminals of individual OSNs expressing a given OR. Using a similar genetic strategy, we provided evidence that ORs have instructive roles in directing axons to their appropriate glomeruli in the bulb (Mombaerts et al., 1996
Initial studies emphasized the stereotyped nature of the glomerular array of the bulb, a view that was based on analysis of the absolute positions of glomeruli corresponding to single ORs (Ressler et al., 1994
Here, we have tagged three genes of the mOR37 subfamily by gene targeting in embryonic stem (ES) cells. The use of two histological axonal markers (Rodriguez et al., 1999
MATERIALS AND METHODS
Targeting vectors. Genomic clones containing mOR37 genes were isolated from a 129/Sv mouse P1 genomic library (Genome Systems, St. Louis, MO). Fragments containing the respective OR37 coding regions were subcloned into pBS-SKII (Stratagene, La Jolla, CA). A PacI site was generated by recombinant PCR three nucleotides downstream of the stop codon of the mOR37A, mOR37B, and mOR37C genes, respectively. A PmeI site was generated at the 3' end of the targeting vectors for linearization of the construct. A PacI fragment containing IRES-taulacZ-LTNL (Mombaerts et al., 1996
Gene targeting. Targeting vectors were linearized with PmeI. Electroporation and culture of E14 cells (Hooper et al., 1987
Targeted ES clones containing the LTNL cassette were transiently transfected with the plasmid expressing the Cre recombinase. Genomic DNA from ganciclovir-resistant clones was digested with EcoRI and analyzed by Southern blot hybridization with a 3' internal probe. Recombinant clones were injected into C57BL6/J blastocysts, and germ line transmission was obtained for mOR37A-ITLZ (clone Y68/Cre8) and mOR37C-ITLZ (clone V37/Cre1).
Targeted ES clones containing the LNL cassette were injected directly into blastocysts, and germ line transmission was obtained for mOR37B-ITLZ-LNL (clone B136), mOR37A-ITGFP-LNL (clone H26), and mOR37C-ITGFP-LNL (clone O44). The neo-selectable marker was subsequently removed by crossing mice heterozygous for the LNL allele to EIIa-Cre transgenic mice (Lakso et al., 1996
5-bromo-4-chloro-3-indolyl-
Immunohistochemistry. Tissues were fixed for 45 min in 2% paraformaldehyde in 0.1 M phosphate buffer, pH 7.4, at 8°C and incubated in 25% sucrose overnight. Sixteen micrometer coronal sections were mounted on Superfrost slides (Fisher, Pittsburgh, PA). For
In situ hybridization on frozen sections. Templates for specific antisense RNA probes were generated by PCR amplification of the 3' noncoding region. Amplification products were subcloned into the pGEM T-vector and sequenced before probe preparation.
5' primers were mOR37A/1, mOR37B/1, and mOR37C/1; 3' primers were mOR37A/2, mOR37B/2, and mOR37C/2 (Strotmann et al., 1999
Mice were killed by CO2 asphyxiation and decapitated. The lower jaw and top of the skull were carefully removed using a bone cutter (Fine Science Tools, Foster City, CA). The tissue was embedded in Tissue-Tek and frozen on dry ice. Coronal sections of 10 µm were cut on a cryostat at
24°C, adhered to Superfrost microslides, and air-dried for 2 hr. For in situ hybridization, tissue sections were covered with 10 µl of hybridization solution containing ~3-5 ng of digoxigenin-labeled RNA and coverslipped. Hybridization and posthybridization washes were performed as by Strotmann et al. (1994)
Quantitative analyses. The relative positions of cell somata within the vertical dimension of the olfactory epithelium were determined as detailed previously (Strotmann et al., 1996
Mice. The analyses were performed on a total of 214 bulbs from 129 different mice. The remaining 44 bulbs were judged uninformative, for instance, because of damage. For each genotype, 16-23 mice were analyzed from three to six litters. Both males and females were used at ages between 10 and 16 weeks.
Microscopy and photography. Whole-mount specimens were photographed using a Wild M8 stereomicroscope. Sections were analyzed with a Zeiss Axiophot microscope. Fluorescence was examined using the appropriate filter sets for Cy3 and EGFP. Overlay of fluorescent images was obtained by double or triple exposure of individual color slides (400 ASA). The fluorescent glomerulus was photographed in whole mount at 400× magnification using an Olympus Optical (Tokyo, Japan) IX70 inverted microscope with fluorescence optics.
RESULTS
Targeted mutagenesis of mOR37 genes
OSNs expressing ORs of the mOR37 subfamily occupy a circumscribed region within the epithelium that overlaps with three of the four expression zones for the other ORs (Strotmann et al., 1992
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Figure 1. Genetic strategy. A, Genomic organization of the mOR37 locus, redrawn from Strotmann et al. (1999)
Patterns of mOR37-expressing neurons
We first confirmed that the spatial patterns of OSNs expressing the mOR37-lacZ alleles match the patterns of OSNs expressing the corresponding wild-type alleles.
Whole-mount specimens of mice that carry either of the three mOR37-lacZ mutations were stained with X-gal. Figure 2A shows a view onto a half-head from a mouse homozygous for the mOR37A-lacZ (henceforth abbreviated A-lacZ) mutation. Blue-colored cell bodies are clustered in a patch in the center of the turbinates. This characteristic pattern also emerges when mice homozygous for the mOR37B-lacZ (B-lacZ) mutation (Fig. 2B) or the mOR37C-lacZ (C-lacZ) mutation (Fig. 2C) were stained. Whole-mount in situ hybridization using a digoxigenin-labeled riboprobe specific for mOR37B labels cell bodies in a wild-type mouse (Fig. 2D) that are located in the same patch as those detected by X-gal staining of a B-lacZ mouse (Fig. 2B). Similar results were obtained using mOR37A- and mOR37C-specific probes (data not shown).
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Figure 2. Patterns of mOR37-expressing OSNs in the nasal cavity. a, Whole-mount view of the right half-head from an A-lacZ mouse stained with X-gal. The nasal septum was removed, allowing a view onto the medial aspect of the turbinates. Blue cells are clustered in a patch in the central region of the turbinates (anterior is to the left, and dorsal is to the top). b, Whole-mount preparation of a B-lacZ mouse stained with X-gal. c, Whole-mount preparation of a C-lacZ mouse stained with X-gal. d, Whole-mount in situ hybridization using a probe specific for mOR37B. Reactive cells are clustered in the same small area of epithelium as those cells expressing the corresponding mutant allele in B-lacZ mice (compare with b). e, f, Adjacent coronal sections through the nasal cavity from a heterozygous B-lacZ mouse stained with X-gal (e) or subjected to in situ hybridization with a probe specific for mOR37B (f). The pattern of cells labeled by either X-gal staining or in situ hybridization is superimposable. Reactive cells are clustered on the tips of endoturbinates II and III and on ectoturbinate 3. Scale bars: e, f, 200 µm.
Coronal sections through the nasal cavity from a heterozygous B-lacZ mouse (Fig. 2E) reveal the characteristic clustering of X-gal-reactive OSNs expressing ORs from the mOR37 subfamily on the tip regions of endoturbinates II and III and on ectoturbinate 3. In adjacent sections of the same specimen (Fig. 2F), in situ hybridization identifies cells expressing mOR37B from the targeted or wild-type allele. Importantly, the spatial patterns of the cells reacting with X-gal histochemistry (Fig. 2E) or in situ hybridization (Fig. 2F) are superimposable; there are no areas that contain only cells reactive with the riboprobe (reflecting either the targeted or wild-type allele) but not with X-gal (representing the targeted allele). Similar results were obtained with A-lacZ and C-lacZ mice (data not shown). Thus, at a gross level, the spatial expression pattern of the mOR37 genes is not affected by targeted insertion of the IRES-taulacZ cassette.
Laminar organization
We have shown previously that, in mature (>6-week-old) mice, OSNs expressing a particular OR gene exhibit an additional level of spatial organization within the olfactory epithelium; the cell bodies are arranged in distinct laminar layers (Strotmann et al., 1996
A cross-section through the epithelium of an A-lacZ mouse after X-gal staining is shown in Figure 3A. The dendrites and axons of individual A-lacZ expressing OSNs appear of similar length, implying that the cell bodies are preferentially located within the middle layer of the epithelium. The relative position within the cellular layer was determined quantitatively for a large number of A-lacZ-expressing OSNs (see Materials and Methods). Their cell bodies are predominantly located within the 0.4-0.6 range (Fig. 3B), with few cells above and below these levels. The laminar preference of OSNs expressing the mOR37A gene was confirmed in wild-type mice by in situ hybridization (Fig. 3C), with quantification of the results shown in Figure 3B.
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Figure 3. Laminar patterning of mOR37-expressing OSNs. a, c, d, f, g, i, Cross-sections of the olfactory epithelium. b, e, h, Graphic representations of the numbers of reactive cells in different laminae; data were collected from randomly selected and informative sections of four mice each. a, A-lacZ mouse stained with X-gal. Reactive cells are located within the middle layer of the epithelium. b, Laminar distribution of cell bodies reactive with X-gal in A-lacZ mice (blue bars) and with a specific probe in wild-type mice (pink bars). c, In situ hybridization of wild-type mouse with a probe specific for mOR37A. d, B-lacZ mouse stained with X-gal. Reactive cells are closer to the luminal surface than A-lacZ cells (see a). e, Laminar distribution of cell bodies reactive with X-gal in B-lacZ mice (blue bars) and with a specific probe in wild-type mice (pink bars). f, In situ hybridization of wild-type mouse with a probe specific for mOR37B. g, C-lacZ mouse stained with X-gal. Reactive cells are located very close to the luminal surface. h, Laminar distribution of neurons reactive with X-gal in C-lacZ mice (blue bars) and with a specific probe in wild-type mice (pink bars). i, In situ hybridization of wild-type mouse with a probe specific for mOR37C. Cells are located in an apical cellular layer, similar to C-lacZ-expressing neurons. Scale bars: (in d) a, d, g, 20 µm; (in f) c, f, i, 20 µm.
Cell bodies expressing the B-lacZ allele (Fig. 3D) are located closer to the luminal surface than A-lacZ cells, within the range of 0.6-0.8 (Fig. 3E). Cells expressing the mOR37B wild-type allele exhibit a similar laminar patterning (Fig. 3F). Finally, OSNs expressing the C-lacZ allele (Fig. 3G) have very short dendrites, because their cell bodies are situated even closer to the luminal surface than mOR37B-expressing OSNs, in a position ~0.8-1.0 (Fig. 3H,I). We note that the linear order of mOR37 genes on the chromosome (A-C-B) does not correspond to the relative laminar positions within the epithelium (A-B-C), eliminating a simple correlation between these variables.
Thus, OSNs expressing a particular mOR37 gene reside within distinct epithelial layers, and this configuration is replicated in the gene-targeted mice. We regard this observation as a sensitive indicator that the targeted insertion of IRES-taulacZ does not alter the pattern of gene expression in a major way.
Mutually exclusive expression of mOR37 genes
It is conceivable that mOR37 genes are coexpressed in some OSNs. The availability of a second histological marker (tauGFP) allows us to examine this issue at the single-cell level. Double-heterozygous mice were generated that carry two differentially tagged mOR37 alleles; expression of tagged alleles residing in trans on homologous chromosomes can be revealed by double-labeling of individual histological sections. Imaging was performed by means of the intrinsic fluorescence of GFP (green) and a Cy3-conjugated secondary antibody to
To ensure that our imaging conditions would adequately identify double-labeled cells, a control experiment was first performed with double-heterozygous mice carrying the OMP-GFP mutation (C. Zheng, P. Feinstein, and P. Mombaerts, unpublished results) and the B-lacZ mutation. In these mice, all mature OSNs express GFP and are green fluorescent, whereas some OSNs express also the B-lacZ allele, resulting in red fluorescence. In double exposure, all B-lacZ neurons appear yellow (Fig. 4A) because of the overlay of the red and green colors, demonstrating that we can detect both markers simultaneously in single cells.
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Figure 4. MOR37 gene- and allele-specific expression patterns. Cross-sections through the olfactory epithelium of double- or compound-heterozygous mice. The genotype is indicated in the top right of each panel. GFP-expressing cells are green fluorescent, lacZ-expressing cells are red fluorescent; cells coexpressing GFP and lacZ are yellow in double exposure. a, OMP-GFP × B-lacZ mouse. All neurons expressing B-lacZ coexpress GFP and are yellow. b, A-GFP × B-lacZ mouse. Two distinct neuronal populations are visible, either green fluorescent (A-GFP) or red fluorescent (B-lacZ). No double-stained neuron is detectable. c, B-lacZ × C-GFP mouse. No cells are both red and green fluorescent. Note that both populations are located rather apically within the epithelium. d, A-lacZ × C-GFP mouse. No double-stained neurons are detectable. Both populations are located in different horizontal layers of the epithelium. e, A-lacZ × A-GFP mouse. The mOR37A gene is strictly subject to monoallelic expression. Two differentially stained neuronal populations are detectable, and no double-stained cells are found. Note the location of cell bodies preferentially in the middle cellular layer of the epithelium. f, C-lacZ × C-GFP mouse. The mOR37C gene is strictly subject to monoallelic expression. Note the location of cell bodies preferentially in the apical cellular layer of the epithelium. Scale bar, 20 µm.
In mice double-heterozygous for the A-GFP and B-lacZ mutations (A-GFP × B-lacZ), double exposure reveals two differentially stained cell populations (Fig. 4B). Among 980 cells analyzed from four mice, no cell was detected expressing both markers. Likewise, analyzing 800 individual fluorescent cells from B-lacZ × C-GFP (Fig. 4C) and A-lacZ × C-GFP crosses (Fig. 4D), we encountered no double-fluorescent cells. Thus, coexpression of mOR37 genes in trans within individual OSNs is very rare, if it occurs at all.
Monoallelic expression
The mouse OR genes I7 and I54 are monoallelically expressed within an individual OSN, from either the paternal or maternal allele, but not both (Chess et al., 1994
In mice compound-heterozygous for the A-lacZ (maternal red) and A-GFP (paternal green) mutations, no yellow (i.e., double-expressing) cells are present among 750 individual cells, analyzed in randomly selected and informative sections of three mice (Fig. 4E). Interestingly, red and green cells are present in equal numbers (average of 47.3% red cells and 52.7% green cells). The reverse experiment in which expression of the maternal allele results in green fluorescence and that of the paternal allele in red fluorescence gives a similar result in 750 analyzed cells (average of 51.1% red cells and 48.9% green cells). Both alleles are thus expressed at equal frequencies in these neuronal populations. Similarly, among 800 fluorescent cells detected in four mice heterozygous for the C-lacZ (maternal) and C-GFP (paternal) mutations (Fig. 4F), no double-fluorescent neurons are detected (average of 54.2% red cells and 45.8% green cells). Thus, these mOR37 genes are subject to monoallelic expression at a high level of fidelity. Moreover, we have characterized this phenomenon for the first time quantitatively and at the single-cell level (Rodriguez et al., 1999
Projection patterns in the olfactory bulb
Having shown in control experiments that the targeted mutagenesis does not alter the expression pattern of the mOR37 genes, we next imaged the glomerular targets of A-lacZ-expressing OSNs with X-gal histochemistry in whole mounts and sections (Fig. 5).
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Figure 5. Glomerular pattern in A-lacZ mice. a, Whole-mount view onto the ventral surface of a bulb removed from the cranial cavity. Stained fibers converge on a single target (anterior is to the left, and medial is to the top). b, Parasagittal section through a bulb stained with X-gal and counterstained with neutral red. A single blue glomerulus is located on the anteroventral aspect of the bulb. The position of the cribriform plate is indicated by the dashed line (anterior is to the left, and dorsal is to the top). c, Higher magnification identifies the stained area as a single glomerulus. d, Coronal section through the left and right bulbs counterstained with neutral red. Individual glomeruli in the ventral and central region of the bulb are stained blue. They are located in similar positions in both bulbs (dorsal is to the top). e, Coronal section through the right bulb from a different A-lacZ mouse. The labeled glomerulus is positioned slightly laterally compared with e. f, Coronal sections through the right bulb. Of 40 sections, the first and last are shown. The center of the first glomerulus, as identified by X-gal staining, is located on section 1, the center of the second glomerulus is located on section 40. They are separated by 34 sections without any glomerular X-gal staining. Both labeled glomeruli are located in the ventral region of the bulb but in slightly different mediolateral positions. Scale bars: b, 400 µm; c, 100 µm; d-f, 300 µm.
On the ventral surface of the bulb, many blue axons converge onto a single target (Fig. 5A). This situation is in contrast with P2-expressing OSNs in P2-IRES-taulacZ mice, which project their axons to at least one glomerulus located within each hemisphere of each bulb (Mombaerts et al., 1996
Figure 5D shows a representative section through the left and right bulbs from another mouse of this strain; on each side, a single stained glomerulus is visible located in a ventral position close to the midline of the bulb. Analysis of 30 bulbs indicates that axons of A-lacZ-expressing OSNs project to a defined domain of the bulb but also reveals variability in the position of the A-lacZ glomeruli; representative results are shown in Figure 5D-F. A small shift to a more medial position (Fig. 5D, left bulb) is found in 27% of bulbs. In 17% of the bulbs, a minor shift laterally is observed (Fig. 5E). This mediolateral shift is within the range of three glomeruli toward each side from the midline. Importantly, it is not symmetrical between the two bulbs from one individual (Fig. 5D). An additional level of variability is that a single labeled glomerulus is detected in 77% of bulbs of A-lacZ mice and two labeled, distinct glomeruli in 23% of bulbs (Table 1). The second glomerulus is always located in close vicinity to the other one, usually 450-500 µm more posterior (Fig. 5F). This distance corresponds to more than five glomerular widths. In all five cases of bulbs with two blue glomeruli for which the contralateral bulb of the same mouse was also characterized, the other bulb had only a single blue glomerulus; in other words, the maximal number of blue glomeruli in a mouse was three.
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Table 1. Percentage of olfactory bulbs with particular numbers of stained glomeruli in mOR37-lacZ strains Thus, axons of A-lacZ-expressing OSNs converge to one or two glomeruli per bulb, which are located in a defined domain but at slightly variable positions. However, a better characterization of glomerular positions involves determining the relative positions of glomeruli corresponding to various members of the mOR37 subfamily.
OR-specific glomeruli
Next we determined the glomerular targets for the other tagged mOR37-lacZ alleles (Fig. 6). The projection pattern of B-lacZ-expressing neurons was analyzed in series of cross-sections through 26 bulbs of B-lacZ mice. In 62% of bulbs, a single stained glomerulus is detected, located in the ventral region (Fig. 6A) in a position very similar to the A-lacZ glomeruli. B-lacZ glomeruli from different bulbs exhibit a mediolateral variability (data not shown), as was described for the A-lacZ mice. In 38% of B-lacZ bulbs, two stained glomeruli are observed (Table 1), with the second glomerulus also located in a similar ventral position of the bulb and separated from the first one by 400-500 µm in the anteroposterior dimension (data not shown). From six cases with two blue glomeruli in one bulb and bilateral analysis, four had also two blue glomeruli in the other bulb.
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Figure 6. Glomerular patterns in mOR37-lacZ mice. Coronal sections through the right bulbs from various mOR37-lacZ strains stained with X-gal (dorsal is to the top, and medial is to the left). a, B-lacZ. The stained glomerulus is located in the ventral and central region of the bulb. b, C-lacZ. The stained glomerulus is also located in the ventral and central region of the bulb. c, A-lacZ × B-lacZ. Two distinct blue-stained glomeruli are visible, which are located adjacent to each other. d, A-lacZ × B-lacZ. Two glomeruli are stained, separated in the mediolateral dimension by one unlabeled glomerulus. e, A-lacZ × B-lacZ. Of six sections, the first and the last are shown. On the first section, a stained glomerulus is visible in the ventral region of the bulb. On the next three sections, the glomerular X-gal staining disappears but reappears on the consecutive ones. Thus, these two glomeruli are located immediately adjacent to each other in the anteroposterior dimension. f, B-lacZ × C-lacZ. Two glomeruli are stained, separated in the mediolateral dimension by two unlabeled glomeruli. g, A-lacZ × C-lacZ. Two stained glomeruli are visible, located adjacent to each other in the mediolateral dimension. Scale bar: (in a) a-d, f, g, represents 200 µm; e, 200 µm.
Analysis of the third targeted allele, C-lacZ, yields similar observations. In 81% of 31 bulbs, a single labeled glomerulus is located in the ventral position of the bulb (Fig. 6B, Table 1). In the remaining 19%, a second stained glomerulus is located 400-500 µm more posterior. From four cases with two blue glomeruli in one bulb and bilateral analysis, two had also two blue glomeruli in the other bulb.
Thus, OSNs expressing distinct ORs of the mOR37 subfamily project their axons to glomeruli that are located within a defined domain of the bulb (ventral and central). We estimate that this domain encompasses ~30 of the 1800 glomeruli (~2%) in the bulb.
Spatial relationships between mOR37 glomeruli
Because the position of the labeled glomeruli varies between individuals, the identity of a glomerulus cannot be deduced from its position. It is thus conceivable that different mOR37 subpopulations project to the same glomeruli.
Therefore, mice double-heterozygous for two distinct mOR37-lacZ mutations were examined. This comparison is valid because mice heterozygous for a particular mOR37-lacZ mutation are indistinguishable from homozygotes (data not shown). Figure 6C shows a coronal section through the bulb of an A-lacZ × B-lacZ mouse. Two distinct glomeruli are now stained, suggesting that OSNs expressing A-lacZ or B-lacZ project to different glomeruli. Analyzing 27 bulbs from this genotype, in no case is only a single glomerulus stained (Table 1). Two and three glomeruli are detectable at similar percentages (48 and 45%, respectively); only in 7%, four stained glomeruli are visible in a single bulb.
Among the 13 bulbs from A-lacZ × B-lacZ mice with two blue glomeruli, in most cases (n = 10, or 77%) they are located side by side. Of these 10 cases, seven cases are adjacent in the mediolateral dimension (Fig. 6C), and in three cases, this relationship pertains to the anteroposterior dimension (Fig. 6E). In the three other bulbs with two labeled glomeruli, they are separated by one unlabeled glomerulus in the mediolateral dimension (Fig. 6D), or two and three unlabeled glomeruli in the mediolateral and anteroposterior dimensions, respectively (data not shown). In the 12 bulbs with three blue glomeruli, the third glomerulus is located 400-500 µm more posterior, comparable with the location of the extra glomerulus observed in mOR37-lacZ mice (Fig. 5F). In nine of these 12 bulbs, two glomeruli are adjacent and the third is separate; in the remaining three bulbs, none of the three labeled glomeruli are adjacent. In the two bulbs with four labeled glomeruli, a second doublet of glomeruli is present with the characteristics described for the first pair (data not shown). Together, these observations suggest that the A and B glomeruli are preferentially adjacent (Table 2).
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Table 2. Percentage of olfactory bulbs with adjacent and separated stained glomeruli in mOR37-lacZ double-heterozygous mice In all 29 bulbs from B-lacZ × C-lacZ mice, more than one glomerulus is stained, suggesting that B-lacZ- or C-lacZ-expressing OSNs also project to different glomeruli (Fig. 6F, Table 1). Two or three glomeruli are identified at similar percentages (52 and 41%, respectively), and four glomeruli are found in only 7% of bulbs. This distribution is comparable with that of the A-lacZ × B-lacZ genotype. A major difference, however, is that in most bulbs with two blue glomeruli (12 of 15, or 80%), they are not adjacent but separated by one to three unlabeled glomeruli (Fig. 6F). Similarly, in eight of 12 bulbs (67%) with three labeled glomeruli, none of them are adjacent. In the two bulbs with four stained glomeruli, these are segregated in two pairs as described for the A-lacZ × B-lacZ cross. Thus, it appears that the B and C glomeruli are usually separated by other glomeruli (Table 2).
Finally, among 31 bulbs of A-lacZ × C-lacZ double-heterozygotes, two glomeruli are stained in 65% of bulbs, three glomeruli in 32% of bulbs, and four glomeruli in a single case. As is the case in the A-lacZ × B-lacZ genotype, in most bulbs with two blue glomeruli (15 of 20, or 75%), they are adjacent to each other (Fig. 6G), and in only a single case of the 10 bulbs with three blue glomeruli, they are all separated. This suggests that the A and C glomeruli are typically adjacent (Table 2).
It is important to note that, from 35 bilaterally characterized cases of the three types of double-heterozygous mOR37-lacZ mice, most mice (n = 29, 83%) have a dissimilar pattern of stained glomeruli between the left and right bulbs; this fraction is probably underestimated because glomeruli for distinct mOR37 genes cannot be distinguished, precluding their unambiguous identification on the basis of their positions.
Thus, axons from mOR37-expressing OSNs project in the bulb to a defined domain in which they target distinct glomeruli at slightly variable positions. In ~80% of bulbs, the A and B glomeruli and the A and C glomeruli are adjacent.
TauGFP as an axonal marker
Our analysis thus far does not permit us to distinguish to which of the two mOR37 genes each labeled glomerulus in the double-heterozygotes corresponds. To determine unambiguously the relative spatial arrangement of particular mOR37 glomeruli and to show that they are innervated by OSNs expressing only one particular mOR37 gene, we made use of the axonal marker tauGFP (Rodriguez et al., 1999
Figure 7A shows a whole-mount preparation of a bulb from an A-GFP mouse. At high magnification, a large number of strongly fluorescent fibers can be seen converging onto a distinct target. On a horizontal section through the nose and bulb of an A-GFP mouse, one green fluorescent target is visible in the anterior region of each bulb (Fig. 7B). The counterstaining with propidium-iodide, which highlights the shell of periglomerular cell bodies, reveals a single labeled glomerulus in each case. Thus, tauGFP is an acceptable substitute for taulacZ to image glomeruli for an mOR37 gene.
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Figure 7. Glomerular patterns in mice with differentially tagged mOR37 alleles and genes. a, Whole-mount view of the bulb from an A-GFP mouse. Intensely fluorescent fiber bundles can be seen converging onto a single glomerulus. b, Horizontal section through the nasal cavity and bulb from an A-GFP mouse, counterstained with propidium-iodide. Individual glomeruli in the two bulbs are green fluorescent, located at similar positions (posterior is to the top). c, Cross-section through the right bulb from an A-GFP × A-lacZ mouse. Axons from neurons expressing mOR37A but different axonal markers terminate in the same glomerulus, resulting in a yellow appearance. A region from the outer nerve layer (small white box) is shown as an inset; here, fibers that are either green fluorescent (arrow) or red fluorescent (arrowhead) are seen approaching the glomerulus separately. d, Cross-section through the right bulb from a C-GFP × C-lacZ mouse. Axons from neurons expressing mOR37C but different axonal markers terminate in the same glomerulus. e, Cross-section through the left bulb from an A-GFP × C-lacZ mouse. Two distinct glomeruli are stained (A-GFP, green; C-lacZ, red), located adjacent to each other. Medial is to the right. f, Cross-section through the left bulb from an A-GFP × C-lacZ mouse. Again, the two glomeruli are located adjacent to each other, but the doublet is displaced in the mediolateral dimension compared with e. Medial is to the right. g, Cross-section through the left bulb from another A-GFP - C-lacZ mouse. The glomeruli are also located next to each other but are arranged in an inverted orientation compared with e and f. Medial is to the right. h, Cross-section through the right bulb from the mouse shown in e. The two labeled glomeruli are separated by two unlabeled glomeruli. The spatial arrangement is thus discordant between the two bulbs of this mouse. Medial is to the left. i, Four coronal sections (16 µm each) through the bulb from an A-GFP × C-lacZ mouse. The approximate center of the C-lacZ glomerulus is located on section 1. On section 4, the C-lacZ glomerulus is still visible; adjacent to it, the A-GFP glomerulus is now visible. These two glomeruli are located immediately next to each other but in a slightly different anteroposterior dimension. Scale bars: a, 100 µm; b, 200 µm; c-i, 50 µm.
It is important to demonstrate that the two populations of OSNs, each expressing a differentially tagged allele of the same mOR37 gene as a result of monoallelic expression, co-converge to the same glomeruli. Coronal sections through the bulbs from compound-heterozygous A-GFP × A-lacZ mice were analyzed. Figure 7C shows a triple exposure of a section counterstained with 4',6-diamidino-2-phenylindole to determine the contours of individual glomeruli. Small numbers of green fluorescent (A-GFP allele) and red fluorescent (A-lacZ allele) fibers are visible in the outer nerve layer. The larger fiber bundles and the glomerulus itself appear intensely yellow because of the overlay of the two colors, indicating that the axons of both neuronal subpopulations intermingle diffusely and terminate within the same glomerulus. Similar results are obtained for neurons expressing differentially tagged mOR37C alleles (Fig. 7D). Thus, targeted integration of the IRES-taulacZ or IRES-tauGFP cassette results in identical projection patterns.
Local permutations in the glomerular array
Next, mice double-heterozygous for the A-GFP and C-lacZ mutations were analyzed to determine the position of the glomeruli relative to each other. Figure 7E shows a cross-section through the bulb from an A-GFP × C-lacZ mouse. The triple exposure reveals two differentially stained glomeruli. At this level of resolution, differentially labeled fibers each form distinct glomeruli, demonstrating that OSNs expressing different mOR37 genes innervate specific glomeruli. The analysis of A-lacZ × C-lacZ mice described above suggested that the A and C glomeruli are usually adjacent, with 65, 32, and 3% of cases of two, three, and four glomeruli, respectively (Table 1). Among 40 bulbs of A-GFP × C-lacZ mice, there are 27 with two glomeruli, 11 with three, and two with four glomeruli; this distribution of 67, 28, and 5% matches that observed in the A-lacZ × C-lacZ cross. Similarly, in 82% of cases with two blue glomeruli, they are adjacent compared with 81% in the A-GFP × C-lacZ. Thus, these observations indicate that the IRES-taulacZ and IRES-tauGFP alleles are functionally equivalent for our purposes and, furthermore, that the projection patterns in gene-targeted mice reflect the wild-type situation.
Because we are here able to distinguish the A and C glomeruli, bulbs with more than two labeled glomeruli can also be included in the analysis. Together, A-GFP and C-lacZ glomeruli are adjacent in 32 of 40 bulbs (80%) analyzed from this cross. In 19 of these 32 bulbs (59%), they are adjacent in the mediolateral dimension. In 11 of these 32 bulbs (34%), the A glomerulus is medial to the C glomerulus, as shown in Figure 7, E and F; the position of the red-green doublet along the mediolateral axis is slightly different in these two examples, confirming the conclusion from the double-lacZ cross (Fig. 6G). In eight of the 32 bulbs with adjacent glomeruli (25%), the two types of glomeruli are arranged in the reverse, lateromedial, orientation (Fig. 7G), the A glomerulus is here located lateral to the C glomerulus. Two stained glomeruli are adjacent in the anteroposterior dimension in 13 of 32 bulbs (41%). In the example shown in Figure 7I, the C glomerulus occupies a position immediately anterior to the A glomerulus; this arrangement is found in six bulbs (19%); the reverse, posteroanterior, orientation is observed in the remaining seven bulbs (22%). As is the case in A-lacZ × C-lacZ genotype, in a minority of bulbs (8 of 40, or 20%), A and C glomeruli are separated by one, two (Fig. 7H), or three unlabeled glomeruli.
The analysis yields similar conclusions when the 27 bulbs of the A-GFP × C-lacZ genotype with only two labeled glomeruli are considered separately. The four combinations of A and C glomerular relationships are 48% for mediolateral, 22% for lateromedial, 15% for anteroposterior, and 15% for posteroanterior. The variable structure of the glomerular array becomes even clearer by analysis of the patterns in the 17 A-GFP × C-lacZ mice with two bulbs characterized. Only eight mice (47%) had the same number of glomeruli in each bulb, and a mere two mice (12%) showed identical numbers (two) and indistinguishable relative positions (mediolateral). This is the best evidence for local permutations in the glomerular array.
Variability
From the large number of possibilities, selected combinations are shown in Figure 8 involving a single glomerulus per mOR37 gene, for the sake of clarity. The quantitative analyses reveal a preferred (~80%) arrangement of the A glomerulus adjacent to the B glomerulus and the C glomerulus, whereas the B and the C glomeruli are most frequently not located next to each other (shown in situations 1-3). In the remaining ~20% of cases, the A glomerulus is neither located adjacent to the B nor to the C glomerulus (shown in situation 4). However, the B and C glomeruli are located adjacent to each other only in these rare cases (also shown in situation 4).
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Figure 8. Local permutations in the glomerular array. Schematic three-dimensional representations of a restricted domain of the glomerular array from the anteroventral region of the right olfactory bulb. This region is located behind the cribriform plate and viewed from the nasal cavity; only a small number of glomeruli is drawn. Selected arrangements of mOR37 glomeruli are depicted, with each mOR37 gene corresponding to a single glomerulus for the sake of clarity and simplicity. The A glomerulus is placed centrally in the three most common situations. A, mOR37A; B, mOR37B; C, mOR37C; a, anterior; p, posterior; m, medial; l, lateral.
DISCUSSION
Axonal projection patterns
This study confirms and extends the concept of glomerular convergence that emerged from studies with indirect methods demonstrating that axons from some OSNs converge to individual glomeruli in mice (Ressler et al., 1993
Although the gross features of the glomerular array are conserved, at a fine scale, the position of a specific type of glomerulus varies among bulbs of different mice. Importantly and shown here for the first time, variable interglomerular relationships are also the case among the two bulbs from one individual (Fig. 7E,H), ruling out that the genetic manipulation itself, genetic background variation, and differences in olfactory experience may underlie the local permutations. The variability in the glomerular array of the main olfactory bulb, however, is much less pronounced and at a much finer scale than in the accessory olfactory bulb in which we have not been able to discern meaningful patterns (Rodriguez et al., 1999
Interestingly, the numbers of glomeruli for individual mOR37 genes show independent variations; the frequencies of bulbs from double mOR37-lacZ mice with two stained glomeruli are equal to the products of the frequencies of bulbs with one stained glomerulus in single mOR37-lacZ mice (Table 2). This finding may mean that these neuronal populations do not influence each other in a major way and that the glomerular array can locally accommodate variations in the total numbers of glomeruli.
We did not observe a correlation between the order of genes on the chromosome and the spatial patterning of glomeruli in the bulb; whereas the gene order is A-C-B, the A and C glomeruli, but not the B and C glomeruli, are usually adjacent. Furthermore, the relative order of the glomeruli does not correspond to that of the laminar patterning (A-B-C), eliminating a simple coupling between laminar distribution and glomerular position. Additionally, no obvious correlation was found between overall OR sequence similarity and glomerular positions; mOR37B and mOR37C have 93% amino acid identity between each other, which is more than to mOR37A (87%), yet their glomeruli are typically located the farthest from each other.
More information is required to try to correlate OR sequences, genomic locations, and glomerular positions. One study (Tsuboi et al., 1999
Other species
Although the antennal lobes of insects contain fewer glomeruli, the glomerular array also exhibits variability. The honeybee, for instance, possesses between 156 and 166 glomeruli (Flanagan and Mercer, 1989
Zebrafish is the vertebrate species with the best characterized bulb (Baier and Korsching, 1994
Variability in the glomerular array
By analogy with the fruit fly (Laissue et al., 1999
Our data do not challenge the concept that the arrangement of axonal projections from the epithelium to the bulb is similar from animal to animal. What is perhaps more remarkable is not the variability described here but the highly recognizable pattern of positions of OR-specific glomeruli within the olfactory bulb. mOR37 glomeruli, for instance, are grouped within a defined domain of the bulb, which we estimate to encompass ~30 of the 2000 glomeruli, or a mere ~1.5% of the surface area.
It is important to realize that the glomerular array is not distorted in a global way when bulbs are compared but is rearranged when examined in greater detail. Bulbs are not simply elongated or compressed in one or more dimensions with regard to one another, but nearest neighbor relationships between glomeruli vary qualitatively.
Implications of the local permutations
The developmental mechanisms that govern axonal convergence likely underlie the local permutations. One interpretation is that, during development, axons of OSNs expressing a given OR may project to a rather broad area of the olfactory bulb and intermingle with axons of OSNs expressing other ORs. We speculate that this broad area may occupy ~1.5% of the surface area of the bulb, in accordance with the observed boundaries of the territory of mOR37 glomeruli in the adult. We further speculate that, as a result of the coalescence of neuropil into discrete glomeruli, the relative positions of the OR glomeruli may occupy virtually any position within that territory as a result of stochastic factors. It will be interesting to examine in detail the coemergence of glomeruli for two differentially tagged mOR37 genes during development. Our speculations are supported by direct evidence for the coalescence of glomerular structures from a broad locus, as described recently in P2-IRES-taulacZ mice (Royal and Key, 1999
We believe that the most important implication of the local permutations is of a practical nature. Many of the earlier methods used to describe odor maps are not performed at a scale of resolution that would be hampered by the local permutations (Buck, 1996
FOOTNOTES Received March 30, 2000; revised June 20, 2000; accepted July 6, 2000.
This work was supported by the Deutsche Forschungsgemeinschaft Leibniz Programm, European Community Project TRANS ERBBIO4 4CT 960593, and the Fonds der Chemischen Industrie (all to J.S. and H.B.). P.F. was supported by postdoctoral fellowships from Bristol Myers Squibb and the National Institutes of Health. P.M. received grant support from the Human Frontier Science Program and the National Institutes of Health, and was an Alfred P. Sloan, Basil O'Connor, Guggenheim, Irma T. Hirschl, Klingenstein, McKnight, Rita Allen, and Searle Scholar or Fellow. We thank Annemarie Walsh, Susan Powell-Hayre, and Ruben Peraza of the Transgenic Service at The Rockefeller University for the efficient generation of chimeric mice. Chen Zheng is acknowledged for the IRES-tauGFP cassette, and Heiner Westphal for EIIa-Cre transgenic mice.
Correspondence should be addressed to Peter Mombaerts, The Rockefeller University, 1230 York Avenue, New York, NY 10021. E-mail: peter{at}mail.rockefeller.edu.
REFERENCES
- Baier H, Korsching S (1994) Olfactory glomeruli in the zebrafish form an invariant pattern and are identifiable across animals. J Neurosci 14:219-230[Abstract].
- Buck L, Axel R (1991) A novel multigene family may encode odorant receptors: a molecular basis for odor recognition. Cell 65:175-187[Web of Science][Medline].
- Buck LB (1996) Information coding in the vertebrate olfactory system. Annu Rev Neurosci 19:517-544[Web of Science][Medline].
- Chess A, Simon I, Cedar H, Axel R (1994) Allelic inactivation regulates olfactory receptor gene expression. Cell 78:823-834[Web of Science][Medline].
- Costanzo R (2000) Rewiring the olfactory bulb: changes in odor maps following recovery from nerve transection. Chem Senses 25:199-205
[Abstract/Free Full Text] .- Flanagan D, Mercer AR (1989) An atlas and 3-D reconstruction of the antennal lobes in the worker honey bee, Apis mellifera L. (Hymenoptera: Apidae) Int J Insect Morphol Embryol 18:145-159.
- Friedrich RW, Korsching SI (1997) Combinatorial and chemotopic odorant coding in the zebrafish olfactory bulb visualized by optical imaging. Neuron 18:737-752[Web of Science][Medline].
- Friedrich RW, Korsching SI (1998) Chemotopic, combinatorial, and noncombinatorial odorant representations in the olfactory bulb revealed using a voltage-sensitive axon tracer. J Neurosci 18:9977-9988
[Abstract/Free Full Text] .- Hooper ML, Hardy K, Handyside A, Hunter S, Monk M (1987) HPRT-deficient (Lesch-Nyhan) mouse embryos derived from germline colonization by cultured cells. Nature 326:292-295[Medline].
- Kubick S, Strotmann J, Andreini I, Breer H (1997) Subfamily of olfactory receptors characterized by unique structural features and expression patterns. J Neurochem 69:465-475[Web of Science][Medline].
- Laissue PP, Reiter C, Hiesinger PR, Halter S, Fischbach KF, Stocker RF (1999) Three dimensional reconstruction of the antennal lobe in Drosophila melanogaster. J Comp Neurol 405:543-552[Web of Science][Medline].
- Lakso M, Pichel JG, Gorman JR, Sauer B, Okamoto Y, Lee E, Alt FW, Westphal H (1996) Efficient in vivo manipulation of mouse genomic sequences at the zygote stage. Proc Natl Acad Sci USA 93:5860-5865
[Abstract/Free Full Text] .- Lin DM, Wang F, Lowe G, Gold GH, Axel R, Ngai J, Brunet L (2000) Formation of precise connections in the olfactory bulb occurs in the absence of odorant-evoked neuronal activity. Neuron 26:69-80[Web of Science][Medline].
- Malnic B, Hirono J, Sato T, Buck LB (1999) Combinatorial receptor codes for odors. Cell 96:713-723[Web of Science][Medline].
- Mombaerts P (1999a) Molecular biology of odorant receptors in vertebrates. Ann Rev Neurosci 22:487-509[Web of Science][Medline].
- Mombaerts P (1999b) Seven-transmembrane proteins as odorant and chemosensory receptors. Science 286:707-711
[Abstract/Free Full Text] .- Mombaerts P, Wang F, Dulac C, Chao SK, Nemes A, Mendelsohn M, Edmondson J, Axel R (1996) Visualizing an olfactory sensory map. Cell 87:675-686[Web of Science][Medline].
- Mori K, Nagao H, Yoshihara Y (1999) The olfactory bulb: coding and processing of odor molecule information. Science 286:711-715
[Abstract/Free Full Text] .- Ressler KJ, Sullivan SL, Buck LB (1993) A zonal organization of odorant receptor gene expression in the olfactory epithelium. Cell 73:597-609[Web of Science][Medline].
- Ressler KJ, Sullivan SL, Buck LB (1994) Information coding in the olfactory system: evidence for a stereotyped and highly organized epitope map in the olfactory bulb. Cell 79:1245-1255[Web of Science][Medline].
- Rodriguez I, Feinstein P, Mombaerts P (1999) Variable patterns of axonal projections of sensory neurons in the mouse vomeronasal system. Cell 97:199-208[Web of Science][Medline].
- Royal SJ, Key B (1999) Development of P2 olfactory glomeruli in P2-internal ribosome entry site-tau-lacZ transgenic mice. J Neurosci 19:9856-9864
[Abstract/Free Full Text] .- Rubin BD, Katz LC (1999) Optical imaging of odorant representations in the mammalian olfactory bulb. Neuron 23:499-511[Web of Science][Medline].
- Shepherd GM (1994) Discrimination of molecular signals by the olfactory receptor neuron. Neuron 13:771-790[Web of Science][Medline].
- Strotmann J, Wanner I, Krieger J, Raming K, Breer H (1992) Expression of odorant receptors in spatially restricted subsets of chemosensory neurones. NeuroReport 3:1053-1056[Web of Science][Medline].
- Strotmann J, Wanner I, Helfrich T, Beck A, Meinken C, Kubick S, Breer H (1994) Olfactory neurones expressing distinct odorant receptor subtypes are spatially segregated in the nasal neuroepithelium. Cell Tissue Res 276:429-438[Web of Science][Medline].
- Strotmann J, Konzelmann S, Breer H (1996) Laminar segregation of odorant receptor expression in the olfactory epithelium. Cell Tissue Res 284:347-354[Medline].
- Strotmann J, Hoppe R, Conzelmann S, Feinstein P, Mombaerts P, Breer H (1999) Small subfamily of olfactory receptor genes: structural features, expression pattern and genomic organization. Gene 236:281-291[Web of Science][Medline].
- Tsuboi A, Yoshihara S, Yamazaki N, Kasai H, Asai-Tsuboi H, Komatsu M, Serizawa S, Ishii T, Matsuda Y, Nagawa F, Sakano H (1999) Olfactory neurons expressing closely linked and homologous odorant receptor genes tend to project their axons to neighboring glomeruli on the olfactory bulb. J Neurosci 19:8409-8418
[Abstract/Free Full Text] .- Vassar R, Ngai J, Axel R (1993) Spatial segregation of odorant receptor expression in the mammalian olfactory epithelium. Cell 74:309-318[Web of Science][Medline].
- Vassar R, Chao SK, Sitcheran R, Nunez JM, Vosshall LB, Axel R (1994) Topographic organization of sensory projections to the olfactory bulb. Cell 79:981-991[Web of Science][Medline].
- Wang F, Nemes A, Mendelsohn M, Axel R (1998) Odorant receptors govern the formation of a precise topographic map. Cell 93:47-60[Web of Science][Medline].
- Zheng C, Feinstein P, Bozza T, Rodriguez I, Mombaerts P (2000) Peripheral olfactory projections are differentially affected in mice deficient in a cyclic nucleotide-gated channel subunit. Neuron 26:81-91[Web of Science][Medline].
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