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The Journal of Neuroscience, May 15, 2001, 21(10):3483-3491
Immature Neurons From CNS Stem Cells Proliferate in Response to Platelet-Derived Growth Factor
Anna Erlandsson, Maria Enarsson, and Karin Forsberg-Nilsson Department of Genetics and Pathology, Rudbeck Laboratory, Uppsala University, SE-751 85 Uppsala, Sweden
ABSTRACT
TOP
ABSTRACT
INTRODUCTION
Identifying external signals involved in the regulation of neural stem cell proliferation and differentiation is fundamental to the understanding of CNS development. In this study we show that platelet-derived growth factor (PDGF) can act as a mitogen for neural precursor cells. Multipotent stem cells from developing CNS can be maintained in a proliferative state under serum-free conditions in the presence of fibroblast growth factor-2 (FGF2) and induced to differentiate into neurons, astrocytes, and oligodendrocytes on withdrawal of the mitogen. PDGF has been suggested to play a role during the differentiation into neurons. We have investigated the effect of PDGF on cultured stem cells from embryonic rat cortex. The PDGF
-receptor is constantly expressed during differentiation of neural stem cells but is phosphorylated only after PDGF-AA treatment. In contrast, the PDGF
-receptor is hardly detectable in uncommitted cells, but its expression increases during differentiation. We show that PDGF stimulation leads to c-fos induction, 5'-bromo-2'deoxyuridine incorporation, and an increase in the number of immature cells stained with antibodies to neuronal markers. Our findings suggest that PDGF acts as a mitogen in the early phase of stem cell differentiation to expand the pool of immature neurons.
Key words: PDGF; neural stem cells; differentiation; neurogenesis; rat; cortex; development
INTRODUCTION
Neurons and glia in the mature CNS originate from precursor cells in the ventricular zone of the fetal brain and spinal cord (Williams et al., 1991
). The division of the precursor cells takes place close to the lumen of the neuroepithelium, after which the postmitotic progeny migrate from the ventricular zone to assume their final positions (Rakic, 1972
Many reports describe the establishment of in vitro culture of precursor cells from the embryonic rat or mouse brain (for review, see Gage, 1998
Identification of external signals involved in the regulation of neural stem cell proliferation and differentiation may allow for improved transplantation methods and augment the treatment of neurodegenerative disorders. Several investigators report that in cell cultures of neuroepithelial progenitor cells, exogenously added growth factors can potentiate survival, induce proliferation, or facilitate commitment or differentiation into mature phenotypes. Besides the mitogenic effect of FGF2, it is well documented that ciliary neurotrophic factor (CNTF) acts to direct multipotent stem cells to an astrocytic fate (Johe et al., 1996
Both PDGF ligands are present within the developing cortex at early embryonic ages. Hutchins and Jefferson (1992)
In the present study we aimed at clarifying the effect of PDGF on cortical neural stem cells in culture. We have found that PDGF-AA treatment increases 5'-bromo-2'deoxyuridine bromodeoxyuridine (BrdU) incorporation, and as a consequence it increases total cell number. PDGF-AA was further shown to delay the differentiation of microtubule-associated protein 2 (MAP2)/TuJ1-positive neural precursor cells, possibly by retaining committed but immature neurons in a proliferative state. Double staining with BrdU antibodies showed that PDGF markedly increased the proportion of proliferating cells that expressed MAP2. These results suggest that PDGF acts to expand a pool of immature neurons.
MATERIALS AND METHODS
Cell culture. Embryonic cortex was dissected in HBSS (Life Technologies, Paisley, Scotland) from timed-pregnant Sprague Dawley rats (B&K, Sollentuna, Sweden) on E15 (E1 was defined as the day of copulatory plug). The tissue was gently triturated, and meninges and larger cell clumps were allowed to sediment for 10 min, after which the cell suspension was centrifuged. The pellet was resuspended in N2-medium (Bottenstein and Sato, 1979
6 cells per 10 cm tissue culture dish that was precoated with poly-L-ornithine (Sigma, St. Louis, MO) and fibronectin (Life Technologies). Fresh FGF2 was added daily, and the medium was changed every other day. When subconfluent, the cells were passaged by scraping with a cell lifter (Costar, Cambridge, MA). After the cells were gently triturated in HBSS, the cell suspension was centrifuged, and the pellet was resuspended in N2-medium and plated. The cells were used for experiments 2-6 d after the first passage. The concentrations of PDGF-AA (Pepro Technologies) used in the experiments were 10 ng/ml for continuous treatment, 20 ng/ml for treatment with a single dose, and 100 ng/ml for shorter exposures (c-fos and protein kinase B (PKB)/c-Akt experiments).
Immunocytochemistry. Cells were fixed in ice-cold acid ethanol (90% ethanol, 5% acetic acid). Endogenous peroxidase was quenched by 0.3% H2O2 in methanol for 20 min followed by washing with PBS and permeabilization and blocking in 0.1% Triton X-100 with 5% normal goat serum (Dako A/S, Glostrup, Denmark) in PBS for 30 min. The cells were incubated for 1-4 hr with primary antibody, washed in PBS, and incubated with biotinylated goat anti-mouse immunoglobulin (Kirkegaard and Perry Laboratories, Gaithersburg, MD) for 30 min-1 hr. After washing with PBS, the reaction was developed with diaminobenzidine (DAB SigmaFast, Sigma). Incorporation of BrdU was detected using anti-BrdU antibodies (Amersham Pharmacia Biotech, Uppsala, Sweden). Cells were pulsed with 10 mM BrdU (Amersham Pharmacia Biotech) for 14 hr before fixation. Stained cells were counted, and the ratio of BrdU-positive cells to total cell number per field at 200× magnification was calculated. Seven parallel fields were counted for each time point, and each time point was performed in duplicate. For the double staining of MAP2 and BrdU, a cell proliferation kit, including a nickel-enhanced peroxidase reaction (Amersham Pharmacia Biotech), was used for detection of BrdU-positive cells together with the regular peroxidase reaction for MAP2 as described above. Duplicate cultures were stained for MAP2, and 10 defined fields of each culture dish were photographed, using a computerized microscope (Axiovert 100TV, Zeiss). After BrdU staining, the same fields were photographed again. Cells counts were determined from photographs of stained cells. Monoclonal anti-MAP2 antibody (clone HM2, Sigma), dilution 1:200, and monoclonal anti-Tuj1 antibody (BabCO, Nordic Biosite AB, Täby, Sweden), dilution 1:50, were used. A polyclonal antibody to nestin was a kind gift from Dr. R. McKay (National Institutes of Health, Bethesda, MD).
Deoxynucleotidyl transferase-mediated biotinylated UTP nick end labeling assay. Cells were grown on poly-L-ornathine and fibronectin precoated glass coverslips. After fixation in 2% paraformaldehyde, pH 7.4, the cells were stained using a terminal deoxynucleotidyl transferase-mediated biotinylated UTP nick end labeling (TUNEL) kit (Roche Diagnostics, Mannheim, Germany). The proportion of cells going through apoptosis was counted in 10 parallel fields, using a fluorescence microscope. Each time point was performed in duplicate.
Cell counting. Cell counting was performed using a Coulter Z1 cell counter (Coulter Electronics, Herpendon, UK). The cells were removed from the dishes by scraping and were then carefully dissociated and counted. Each experiment was performed in triplicate.
Northern blot analysis. Total RNA was isolated using the LiCl/urea method (Auffrey and Rougeon, 1980
Western blot analysis: total lysate. Cells were lysed in boiling 2× Laemmli sample buffer (4% SDS, 20% glycerol, Tris-HCl, pH 6.8) and sonicated, and the cell debris was removed by centrifugation. Protein samples were boiled with 5% betamercaptoethanol and loaded to the wells of a 8-16% gradient Tris-glycine gel (Novex). For immunoblotting, the samples were electrically transferred onto a nitrocellulose membrane (Hybond ECL Nitrocellulose membrane, Amersham Pharmacia Biotech) according to the protocol provided by the manufacturer. Nonspecific protein binding to the filter was blocked by incubation in Tris-buffered saline/Tween (TBS-T: 20 mM Tris base, pH 7.6, 137 mM NaCl, 0.2% Tween-20) containing 5% BSA for 1 hr at room temperature, followed by incubation with primary antibody overnight at 4°C. After washing with TBS-T, the membrane was incubated with peroxidase-conjugated secondary antibody (Amersham Pharmacia Biotech), dilution 1:5000, for 1 hr at room temperature. After the filter was washed in TBS-T, the blot was developed using an enhanced chemiluminescence (ECL) system (Amersham Pharmacia Biotech). Before rehybridization of the filters, the membrane was dehybridized (20% SDS, Tris-HCl, pH 6.8, 340 µl betamercaptoethanol) at 55°C for 30 min and washed in TBS-T. Primary antibodies were anti-PKB/c-Akt antibody (Akt1 #06-558, Upstate Biotech) and anti-phosphorylated-PKB/c-Akt antibody (Ser-473-PhosphoAkt #9271S, New England Biolabs), diluted 1:1000.
Western blot analysis: immunoprecipitation. Cell pellets were solubilized in lysis buffer (1% Triton X-100, 20 mM Tris-HCl, pH 7.5, 150 mM NaCl, 2 mM EDTA, 1% aprotinin, 2 mM phenylmethylsulfonyl fluoride, 250 µM orthovanadate) on ice for 5 min, after which cell debris was removed by centrifugation. Immunoprecipitation was performed using 150 µg protein. Lysates were incubated with 10 µl anti-PDGF
Statistical analysis. For statistical analysis we used ANOVA followed by Fisher's multiple comparison. Values presented are the means ± SEM.
RESULTS
Expression of PDGF receptors during stem cell differentiation
The cell culture system used in this study has been described in detail elsewhere (Johe et al., 1996
Western blot analysis was used to investigate the expression and phosphorylation of PDGF receptors in cultured neuroepithelial cells during differentiation (Fig. 1). Proliferating cells were compared with cells differentiating after withdrawal of FGF2. Cell lysates were prepared from proliferating stem cells (FGF2) and cortical stem cells at 1, 2, 4, and 6 d after FGF2 withdrawal (Fig. 1, No add.). Each cell lysate was first immunoprecipitated with antibodies to PDGF
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Figure 1. Western blot analysis was used to study PDGF receptor expression and phosphorylation in CNS stem cells during differentiation. Proliferating cells (FGF2) were compared with differentiating cells after FGF2 withdrawal (No add.). All cell lysates were immunoprecipitated with a PDGF
PDGF
Expression of PDGF receptors after PDGF-AA stimulation
Because PDGF has been implicated in neuronal differentiation of CNS stem cells, we next investigated how receptor levels were influenced by differentiation in the presence of PDGF. Western blot analysis was used to study expression and phosphorylation of PDGF receptors in neuroepithelial cells after PDGF-AA stimulation. Subconfluent cortical stem cells were grown for 1, 2, 4, and 6 d after withdrawal of FGF2, in the presence of 10 ng/ml of PDGF-AA, and compared with proliferating stem cells (FGF2). Because previous investigators (Williams et al., 1997
Figure 2A shows that the PDGF
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Figure 2. Western blot analysis of PDGF receptor expression and phosphorylation in CNS stem cells after PDGF-AA stimulation. Immunoprecipitation of cell lysates was performed using antibodies to PDGF
After PDGF-AA stimulation, phosphorylation of the PDGF
Similarly to cortical stem cells grown in the absence of PDGF, the PDGF
PDGF induces c-fos RNA expression
In our examination of cellular events initiated by activation of PDGF receptors, we next studied expression of the immediate-early gene c-fos in response to PDGF-AA. We first incubated cortical stem cell cultures for 3 hr in N2-medium without FGF2 to minimize possible c-fos background expression. Cells were then stimulated with PDGF-AA and harvested for RNA preparation after 10 min, 30 min, 1 hr, and 2 hr. Control cells were incubated for 3 hr without FGF, after which RNA was prepared. In Figure 3 the ratio of c-fos RNA to GAPDH RNA is shown. The PDGF-AA-stimulated c-fos expression displayed a maximal induction of 50-fold the control level at 1 hr. At 2 hr the amount of c-fos RNA had declined but had not yet reached that of control cells.
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Figure 3. Northern blot analysis of c-fos expression in cortical stem cells after PDGF-AA stimulation. Cell cultures were incubated without FGF2 for 3 hr, stimulated with PDGF-AA, and harvested for RNA preparation after 10 min, 30 min, 1 hr, and 2 hr. The levels of RNA expression are given as the ratios of c-fos to GAPDH pixels.
PDGF-AA stimulation of BrdU incorporation
To investigate whether PDGF-AA can stimulate DNA synthesis in cortical stem cells, cultures were pulse labeled with BrdU for 14 hr and stained with an anti-BrdU antibody. The BrdU incorporation in FGF2-cultured cells were 86 ± 2.9% (data not shown). Cells grown in the absence of FGF2 were compared with cells treated once with PDGF-AA, at the time of FGF2 withdrawal, and cells treated continuously with PDGF-AA (Fig. 4). Counting of BrdU-positive cells revealed that treatment with PDGF-AA gives a fourfold increase in BrdU incorporation 2 d after stimulation, compared with control cultures that received no PDGF. In cultures receiving a single dose of PDGF, the amount of cells incorporating BrdU had declined to that of control cells after 4 d. In cells treated continuously with PDGF-AA, the number of BrdU-positive cells remained significantly higher after 4 d. In all PDGF-treated cells, BrdU incorporation levels had declined to that of control cells after 6 d.
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Figure 4. BrdU labeling of PDGF-AA-stimulated cortical stem cells. Parallel stem cell cultures were untreated, treated once with PDGF-AA, or treated continuously with PDGF-AA for 2, 4, and 6 d. Before fixation the cells were exposed to BrdU for 14 hr. Incorporation of BrdU was detected using anti-BrdU antibodies. Stained cells (duplicate dishes) were counted (in seven parallel fields; 200× magnification) and plotted as the ratio of BrdU-positive cells to the total cell number. *** denotes p < 0.001.
PDGF-AA treatment increases the total cell number
To further clarify the mitogenic effect of PDGF-AA on cortical stem cells, we performed cell counting experiments. FGF2-treated cells were harvested and counted at the start of the experiment. After FGF2 withdrawal, cultures were left untreated, treated once with PDGF-AA, or treated continuously with PDGF-AA. The total cell number was measured using a Coulter Z1 cell counter, on days 2, 4, 6, and 8 (Fig. 5). A fourfold increase in total cell number was observed in cortical cultures treated continuously with PDGF-AA, compared with untreated cultures, at day 8. The increase in total cell number was lower, but significant, in cultures treated with a single dose of PDGF-AA.
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Figure 5. Total cell number in cortical cultures after PDGF-AA treatment. FGF2-treated cells were harvested and counted at the start of the experiment. After FGF2 withdrawal, parallel stem cell cultures were untreated, treated with a single dose of PDGF-AA, or treated continuously with PDGF-AA for 2, 4, 6, and 8 d. The total cell number was measured using a Coulter Z1 cell counter. * denotes p < 0.05, ** p < 0.01, and *** p < 0.001.
PDGF treatment retains an immature phenotype that can be stained with neuronal markers
To further investigate the effect of PDGF on cortical stem cell differentiation, cells were stained with antibodies specific to the neuronal marker, MAP2. Cells were grown for 2, 4, or 6 d without FGF2 in the absence or presence of PDGF. Parallel cultures were untreated, received a single dose of PDGF-AA, or received a daily dose of PDGF-AA (Fig. 6). After fixation, the cells were stained with MAP2 antibodies to reveal neuronal differentiation. Parallel cultures were stained with antibodies to nestin. At the time of FGF2 withdrawal, close to 100% of the cells were nestin positive, and this number declined during the course of the experiment, as the cells differentiated (data not shown). In cultures grown in the presence of FGF2, very few MAP2-positive cells were seen (Fig. 6A). In untreated cultures, MAP2-positive cells started to appear 2 d after FGF withdrawal, indicative of neuronal differentiation (Fig. 6B). The intensity of MAP2 staining in control cultures increased with time (Fig. 6E,H), and 6 d after FGF2 withdrawal (H) most the cells had differentiated. The MAP2-positive neurons now had a more mature phenotype with extended processes (Fig. 6H).
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Figure 6. Staining of PDGF-AA-treated cortical stem cells with neuronal markers. After fixation, the cells were stained with MAP2 antibodies. Control cells, grown in the presence of FGF2, were fixed at the start of the experiment (A). Cells were grown for 2 d (B-D), 4 d (E-G), or 6 d (H-J) without FGF2 in the absence or presence of PDGF. Parallel cultures were untreated (B, E, H), received a single dose of PDGF-AA (C, F, I), or PDGF-AA was added daily (D, G, J). The cells were photographed at 200× magnification.
Cells treated with a single dose of PDGF-AA showed the same type of differentiation pattern (Fig. 6C,F,I), but the differentiation process was slightly slower, with more immature MAP2-positive cells at days 2 and 4 than in the untreated cultures. In contrast, the MAP2-positive cells in cultures treated continuously with PDGF-AA did not differentiate into process-extending neurons (Fig. 6D,G,J) during the course of 6 d. Still, at the end of the experiment (J) only a few cells showed morphological maturation. The MAP2-positive cells rather display a rounded morphology, and many cells appear in clusters. Together with the data from the BrdU incorporation and cell counting experiments, these results manifest a mitogenic effect of PDGF-AA on cortical stem cells during the early stage of neuronal differentiation. Parallel experiments were stained with an antibody to Tuj1, another commonly used neuronal marker, and an identical staining pattern was observed (data not shown).
MAP2-positive progenitor cells incorporate BrdU in PDGF-AA-treated cultures
To further investigate whether dividing cells are neuronal precursors, we used double staining of cortical stem cells with antibodies to MAP2 and BrdU (Fig. 7). Cells were grown for 2 or 4 d without FGF2 in the absence or presence of 10 ng/ml PDGF-AA. Before fixation, the cells were pulse labeled with BrdU for 14 hr. The cells were first stained for MAP2 and photographed, using a computerized microscope. After subsequent staining with BrdU antibodies, the same fields of the culture dish were photographed again. A high proportion of cells grown in the absence of PDGF for 2 d were MAP2 positive (Fig. 7A) and BrdU negative (Fig. 7B, Table 1) and extended short processes. Cells grown in the presence of PDGF-AA showed an undifferentiated phenotype 2 d after FGF2 withdrawal (Fig. 7C). Staining with antibodies to BrdU indicated that a population of the cells treated with PDGF-AA for 2 d were positive for both MAP2 and BrdU (Fig. 7D, Table 1). After 4 d of differentiation, the PDGF-treated cells still showed an immature phenotype, but the amount of double-positive cells had decreased to control levels (Table 1). Untreated cells had a more differentiated phenotype with long processes (data not shown).
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Figure 7. MAP2 and BrdU double staining of PDGF-AA-treated cortical cells. Stem cell cultures were untreated (A, B) or treated with PDGF-AA (C, D) for 2 d after FGF2 withdrawal. The cells were exposed to BrdU for 14 hr, fixed, and stained with MAP2 antibodies (A, C). Defined fields of the culture dish were photographed using a computerized microscope. The cultures were subsequently stained for BrdU, and the same fields were photographed again (B, D).
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Table 1. Percentage of MAP2 and BrdU double-positive cells PDGF-AA is involved in control of stem cell survival
We next investigated whether the increase in cell number, seen after addition of PDGF-AA, results from increased cell survival in addition to the effect on proliferation. Staining for apoptotic nuclei using a TUNEL assay revealed no differences between PDGF-AA and untreated cultures at 2 d of differentiation, but at day 4 we detected a twofold decrease in TUNEL staining in PDGF-AA-treated cultures (Fig. 8A).
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Figure 8. Cell survival studies of PDGF-AA-treated cortical stem cells. A TUNEL assay was performed on cells grown for 2 and 4 d in the presence or absence (no addition, no add.) of PDGF-AA. Cells grown in the presence of FGF2 were included as a control. Cells going through apoptosis were counted using a fluorescence microscope and plotted as the ratio to total number of cells (A). *** denotes p < 0.001. Western blot analysis was used to study PKB/c-Akt phosphorylation in CNS stem cells after FGF2 and PDGF-AA stimulation compared with untreated cells (no add.) (B). Total cell lysates were used for immunoblotting with anti-PKB/c-Akt antibody (Akt) and anti-phosphorylated PKB/c-Akt antibody (p-Akt).
The serine threonine kinase PKB/c-Akt has been linked to increased cell survival. In addition to the TUNEL assay, we studied the phosphorylation of PKB/c-Akt as a measure of increased cell survival. Proliferating stem cell cultures were stimulated for 5 min with 100 ng/ml PDGF-AA or 100 ng/ml FGF2, and total protein lysates were performed. Cell lysates from parallel cultures, receiving only medium, were used as a control. Figure 8B shows that PDGF-AA stimulated a moderate PKB/c-Akt phosphorylation. FGF2 treatment also resulted in stimulation of PKB/c-Akt phosphorylation, but the level was lower than in PDGF-treated cultures. No phosphorylated PKB/c-Akt was detected in untreated control cells. Total PKB/c-Akt levels were not found to be affected by PDGF-AA or FGF2 stimulation.
DISCUSSION
Multipotent neural stem cells can be maintained in monolayer cultures in the presence of FGF2, and on withdrawal of the mitogen give rise to neurons, astrocytes, and oligodendrocytes (for review, see McKay, 1997
The influence of exogenous factors on differentiation of CNS stem cells has been addressed in several studies. Although the pathway of astrocytic differentiation induced by CNTF has been studied in detail (Bonni et al., 1997
PDGF is active either as a homodimer (AA, BB) or heterodimer (AB). Activation of PDGF receptors is brought about by ligand binding to dimerizing receptor subunits
Tyrosine phosphorylation of the PDGF
In contrast to the PDGF
Expression of immediate-early genes is induced in various tissues in response to a wide range of extracellular cues, for example, PDGF (Cochran and Weissman, 1984
From MAP2 (and Tuj1) staining of differentiating cortical cultures, it is evident that exogenous PDGF has a profound effect on cell morphology. The presence of PDGF-AA after withdrawal of FGF2 almost completely abolished morphological neuronal maturation. Instead of extending elaborate processes as in control cultures, PDGF-AA-treated cells retained a rounded immature phenotype even after 6 d. It is interesting to note that a single dose of PDGF, at the time of FGF2 withdrawal, is not sufficient to obtain the same effect on differentiation. Cell culture medium is changed every second day, and any residual PDGF is washed out from the culture at that time. Thus, the presence of PDGF over several days of differentiation is needed for the effect reported in this investigation.
The immature morphology of PDGF-AA-treated stem cell cultures could be caused by a block in differentiation, a continuous proliferation, or an increase in cell survival, or a combination of these. Previous investigators (Johe et al., 1996
The possible effect of PDGF-AA on cell survival was investigated. TUNEL staining revealed that the amount of cells going through apoptosis during the earliest phase of differentiation (2 d) is similar between control and PDGF-treated cultures. At 4 d of differentiation, however, the number of apoptotic nuclei was reduced by half in the presence of PDGF. Activation of PKB/c-Akt has been linked to reduced apoptosis (Kandel and Hay, 1999
Our results fit with the notion that PDGF stimulates a neuronal fate of stem cells, although not as an instructive agent but rather to expand a pool of immature neurons before their morphological maturation occurs.
PDGF-AA and the
FOOTNOTES Received June 12, 2000; revised Feb. 12, 2001; accepted Feb. 23, 2001.
This study was supported by grants from the Swedish Cancer Foundation, the Children Cancer Foundation of Sweden, Magnus Bergwall's Foundation, Wiberg's Foundation, and Selander's Foundation. We thank M. Lindström for excellent technical assistance.
Correspondence should be addressed to Karin Forsberg-Nilsson, Department of Genetics and Pathology, Rudbeck Laboratory, Uppsala University, SE-751 85 Uppsala, Sweden. E-mail: karin.nilsson{at}genpat.uu.se.
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Extracellular Signal-Regulated Protein Kinase Signaling Is Uncoupled From Initial Differentiation of Central Nervous System Stem Cells to Neurons
Mol. Cancer Res., December 1, 2002; 1(2): 147 - 154.
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