The Journal of Neuroscience, August 15, 2001, 21(16):6457-6457
ERRATUM
In the article "The OMP-lacZ Transgene
Mimics the Unusual Expression Pattern of
OR-Z6, a New Odorant Receptor Gene on Mouse Chromosome 6:
Implication for Locus-Dependent Gene Expression," by Martina Pyrski,
Zheng Xu, Eric Walters, Debra J. Gilbert, Nancy A. Jenkins, Neal G. Copeland, and Frank L. Margolis, which appeared on pages 4637-4648 of the July 1, 2001 issue, Figure 6 was incorrectly printed as a black and white figure. The correct color version of the
figure, as well as the legend, is printed here.
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Figure 6.
The three bright-field images illustrate the
criteria by which olfactory neurons coexpressing the
lacZ transgene and OR-Z6 were identified.
OR-Z6 in situ hybridization was performed using a
digoxigenin-labeled OR-Z6 riboprobe, and hybridization
signals were detected and visualized using an alkaline
phosphatase-conjugated anti-digoxigenin antibody and a substrate that
developed a purple precipitate. Left, Hybridization
signals were most prominent in the apical cytoplasmic portion and
appeared triangular (arrows). In addition
we observed a thin line surrounding the nucleus; the
nucleus itself was usually white. Right,
In contrast, ORNs that solely express the lacZ transgene
(arrow) were gray, including the
cytoplasm covering the nucleus. Expression of  -galactosidase was
detected using a primary anti--galactosidase antibody and visualized
using a substrate yielding a gray precipitate.
Middle, The double-labeled neuron shows a combination of
both features described. The gray cytoplasm covering the
nucleus indicates -galactosidase expression (arrow),
whereas the peak-shaped purple precipitate in the apical cytoplasm
corresponds to OR-Z6 in situ hybridization
(arrowhead). The three differently labeled neurons shown
derive from the same coronal cryosection of a 35-d-old
H-lacZ6 mouse and were closely associated on
endoturbinate-II. Scale bar, 20 µm.
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