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 Previous Article

The Journal of Neuroscience, February 1, 2001, 21(3):1076-1085

Adenosine-Mediated Presynaptic Modulation of Glutamatergic Transmission in the Laterodorsal Tegmentum

Elda Arrigoni1, Donald G. Rainnie1, 2, Robert W. McCarley1, and Robert W. Greene1

1 Harvard Medical School and Veterans Administration Medical Center, Department of Psychiatry, Brockton, Massachusetts 02401, and 2 Emory University, Department of Psychiatry, Atlanta, Georgia 30322


    ABSTRACT
TOP
ABSTRACT
INTRODUCTION
MATERIALS AND METHODS
RESULTS
DISCUSSION
REFERENCES

The laterodorsal tegmentum (LDT) neurons supply most of the cholinergic tone to the brainstem and diencephalon necessary for physiological arousal. It is known that application of adenosine in the LDT nucleus increases sleep in vivo (Portas et al., 1997) and directly inhibits LDT neurons in vitro by activating postsynaptic adenosine A1 receptors (Rainnie et al., 1994). However, adenosine effects on synaptic inputs to LDT neurons has not been previously reported. We found that both evoked glutamatergic EPSCs and GABAergic IPSCs were reduced by adenosine (50 µM). A presynaptic site of action for adenosine A1 receptors on glutamatergic afferents was suggested by the following: (1) adenosine did not affect exogenous glutamate-mediated current, (2) adenosine reduced glutamatergic miniature EPSC (mEPSC) frequency, without affecting the amplitude, and (3) inhibition of the evoked EPSC was mimicked by the A1 agonist N6-cyclohexyladenosine (100 nM) but not by the A2 agonist N6-[2-(3,5-dimethoxyphenyl)-2-(methylphenyl)-ethyl]-adenosine (10 nM).

The A1 receptor antagonist 8-cyclopentyltheophylline (CPT; 200 nM) potentiated the evoked EPSCs, suggesting the presence of a tonic activation of presynaptic A1 receptors by endogenous adenosine. The adenosine kinase inhibitor, 5-iodotubercidin (10 µM), mimicked adenosine presynaptic and postsynaptic effects. These effects were antagonized by CPT or adenosine deaminase (0.8 IU/ml), suggesting mediation by increased extracellular endogenous adenosine. Together, these data suggest that the activity of LDT neurons is under inhibitory tone by endogenous adenosine through the activation of both presynaptic A1 receptors on excitatory terminals and postsynaptic A1 receptors. Furthermore, an alteration of adenosine kinase activity modifies the degree of this inhibitory tone.

Key words: adenosine; evoked EPSC; synaptic modulation; laterodorsal tegmental (LDT) nucleus; sleep; electrophysiology; A1 receptors; adenosine kinase inhibitor


    INTRODUCTION
TOP
ABSTRACT
INTRODUCTION
MATERIALS AND METHODS
RESULTS
DISCUSSION
REFERENCES

Cholinergic neurons of the mesopontine laterodorsal tegmental nucleus (LDT) and pedunculopontine tegmental nucleus (PPT), together with the cholinergic neurons of the basal forebrain (BF), constitute to the cholinergic "arousal system." Most of these cholinergic neurons selectively increase their discharge rate during the two states of electroencephalographic (EEG) activation: waking and rapid eye movement (REM) sleep (Szymusiak and McGinty, 1989; Semba, 1991; Jones, 1993; Szymusiak, 1995). Cortical EEG activation and behavioral arousal depends, at least in part, on excitatory cholinergic projections to the thalamus. An increased cholinergic tone can depolarize thalamocortical neurons, inducing a voltage-dependent shift in their discharge pattern from a burst firing mode to a more responsive single-spike mode, which is associated with cortical activation (Steriade et al., 1993). During the transition from waking to sleep, the firing rate of LDT/PPT neurons markedly decreases, reducing the cholinergic tone of their target sites and thus facilitating the transition to sleep (Steriade et al., 1990). Several "sleep factors" have been proposed to regulate the transition from waking to sleep. One such factor, adenosine, has been shown to promote sleep and increase EEG slow wave activity in a manner similar to that observed at the onset of sleep (for review, see Radulovacki, 1985; Strecker et al., 2000). Moreover, adenosine antagonists, such as caffeine and theophylline, are known to suppress sleep (for review, see Fredholm et al., 1999). We recently proposed that adenosine may mediate these state-dependent changes by a direct A1 receptor-mediated inhibition of neurons of the cholinergic arousal system (Rainnie et al., 1994). This hypothesis is supported by subsequent observations that (1) local administration of adenosine into either the LDT/PPT or the cholinergic area of the BF in cats (Portas et al., 1997) and in BF in rats (Basheer et al., 1999) decreases waking, (2) local administration of the adenosine transport inhibitor S(4-nitrobenzyl)-6-thioinosine (NBTI) into the BF promotes sleep in cats (Porkka-Heiskanen et al., 1997), whereas application in the BF of adenosine A1 receptor antagonist increases waking (Strecker et al., 2000), (3) sleep deprivation induces a region-specific increase in BF adenosine levels that decline after subsequent recovery sleep (Porkka-Heiskanen et al., 1997; Basheer et al., 1999; for review, see Strecker et al., 2000), and (4) A1 receptor activation inhibits the firing activity of wake active neurons in the BF of rats and cats (Alam et al., 1999; Thakkar et al., 1999).

Adenosine inhibits 60-70% of LDT/PPT neurons recorded in whole-cell patch-clamp configuration by activation of postsynaptic A1 receptors coupled to an inwardly rectifying K+ conductance (Rainnie et al., 1994). However, the activity of 100% of LDT/PPT and BF neurons recorded with extracellular recording electrodes in vitro was inhibited by similar concentrations of adenosine, suggesting an additional presynaptic locus of adenosine action. In the present study, we have investigated the role of exogenous and endogenous adenosine in regulating synaptic transmission in the LDT nucleus.


    MATERIALS AND METHODS
TOP
ABSTRACT
INTRODUCTION
MATERIALS AND METHODS
RESULTS
DISCUSSION
REFERENCES

Slice preparation. Experiments were performed in vitro on coronal brainstem slices that were prepared using standard methods (Luebke et al., 1993). Briefly, female Long-Evans hooded rats (21-30 d old) were decapitated after isofluorane-induced anesthesia, and their brain were rapidly removed. Coronal slices (400 µm) were cut with an EMS 3000 Vibratome (Electron Microscopy Science, Fort Washington, PA) at 4°C in artificial CSF (ACSF). Two or three slices containing the LDT nucleus were transferred to a holding chamber and incubated at room temperature in continuously oxygenated ACSF for ~1 hr before use.

Recording. Individual slices were transferred to the recording chamber where they were submerged and perfused with pregassed ACSF (1.8 ml/min) at 32°C. Recordings were made with the "blind" whole-cell technique (Blanton et al., 1989) in voltage-clamp mode using an Axopatch-1D amplifier (Axon Instruments, Foster City, CA). Data acquisition and analysis were conducted with a Digidata 1200B interface and Clampex 8.0 software (Axon Instruments). Patch electrodes were pulled from borosilicate glass tubing (outer diameter 1.5 mm; inner diameter 0.86 mm, with filament) on a P97 pipette puller (Sutter Instrument, Novato, CA) and had resistances of 7-10 MOmega when filled with the recording medium. Series resistance was monitored at regular intervals throughout the course of the experiments with voltage pulses (-10 mV; 100 Hz).

Evoked (ev) EPSCs and evIPSCs were electrically induced after stimulation of afferents to the LDT with a bipolar electrode PK/3 (FHC, Bowdoinham, ME) placed on the surface of the slice close to the dorsolateral border of the ipsilateral LDT, rostral-caudal extent (between -8.72 and -9.30 mm relative to Bregma). The regions of the stimulation correspond to the cuneiform nucleus (rostral sections) and parabrachial nucleus (caudal sections). An isolated constant-current source, Master-8 (A.M.P.I. Jerusalem, Israel), was used to generate square-wave pulses of direct current (300-3000 µA; 200 µsec duration; 0.3 Hz). The stimulation trigger was controlled by Clampex 8.0 software. Unless specified, all evEPSCs were captured at Vh -60 mV and are displayed as averages of 10 current traces.

Spontaneous, miniature EPSCs (mEPSCs) were recorded in the presence of tetrodotoxin (TTX; 1 µM). Continuous recordings of 60 sec epochs were obtained with Clampex 8.0 software, low-pass-filtered at 1 or 2 kHz, and digitized at 5 kHz. Events were semiautomatically analyzed off-line with Mini Analysis 4.0 software (Synaptosoft, Leonia, NJ). Traces were then visually examined, and erroneous events were rejected. Events were ranked by amplitude and inter-event interval for preparation of cumulative probability distribution.

Data were compared statistically with either the nonparametric Kolmogorov-Smirnov test (K-S test) or Student's t test. Significance was assessed at p < 0.05. All data are expressed as mean ± SE. To establish whether the evEPSC and evIPSC amplitudes were affected by drug application, the average amplitude of 10 evEPSCs/evIPSCs recorded under control conditions and 10 evEPSCs/evIPSCs recorded during the drug application were compared by using the Student's t test (unpaired). Significance was assessed (p < 0.05) to select "responding" neurons. The amplitudes of the evEPSC/evIPSC for the remaining neurons (termed "nonresponding") were not significantly different from control (p > 0.1), and so they were excluded from the averaging of the response amplitude.

Solutions and drugs. The control ACSF solution for slice preparation and recording contained (in mM): NaCl 124, KCl 2, KH2PO4 3, MgCl2 1.3, CaCl2 2.5, NaCO3 26, glucose 10, pH 7.35, and 315-320 mOsm when gassed with O2 95% and CO2 5%. The patch recording medium contain (in mM): K-gluconate 120, KCl 10, MgCl2 3, HEPES 10, MgATP 2, NaGTP 0.2, pH 7.2, adjusted with KOH; 280 mOsm. The drugs used in these experiments were adenosine, adenosine deaminase (type VI), D(-)-2-amino-5-phosphonopentanoic acid (APV), (-)-bicuculline methiodide (BMI), N6-cyclohexyladenosine (CHA), CPT; 6,7- dinitro-quinoxaline-2,3-dione (DNQX), N6-[2-(3,5-dimethoxyphenyl)- 2-(methylphenyl)-ethyl]adenosine (DPMA), dipyridamole, glutamate, 5-iodotubercidin (ITU), NBTI, and TTX. Drugs were all obtained from Sigma/RBI (Natick, MA). The stocks of CPT, dipyridamole, DPMA, DNQX, ITU, and NBTI were dissolved in dimethyl sulfoxide (DMSO) before being added to ACSF (final concentration of DMSO <0.1%). The ITU stock was protected from the light and added to the ACSF in a perfusion tube protected from ambient light exposure. In some experiments, glutamate (1 mM) was transiently applied (0.8-1.5 sec) by pressure ejection from a micropipette (1-2 µm tip diameter) positioned near the recorded cell using a picospritzer (General Valve, Fairfield, NJ). Allura red food dye (Durkee, San Francisco, CA) was added to the micropipette (2 µl in 2 ml ACSF) to monitor the area of the slice involved in the pressure injection protocol. This dye by itself had no electrophysiological effects on LDT neurons (n = 2).


    RESULTS
TOP
ABSTRACT
INTRODUCTION
MATERIALS AND METHODS
RESULTS
DISCUSSION
REFERENCES

Adenosine inhibits both excitatory and inhibitory evoked synaptic transmission in LDT neurons

Adenosine has a direct effect on LDT excitability by activation of postsynaptic adenosine A1 receptors (Rainnie et al., 1994); however, the effect of adenosine on the synaptic input to these neurons was not known. In the present work we addressed this issue. Electrical stimulation of the ipsilateral border of the LDT (see Materials and Methods) induced a reproducible evEPSC in most of the LDT neurons recorded (86%; n = 92; Vh = -60 mV). Application of non-NMDA and NMDA receptor subtype specific antagonists DNQX (20 µM) and APV (100 µM) completely blocked the evEPSCs in all neurons recorded (n = 27), indicating that they were mediated by glutamate release (Fig. 1A), similar to that observed in guinea pig (Sanchez and Leonard, 1996). These results are consistent with anatomical data that LDT neurons receive glutamatergic projections (for review, see Semba, 1999) and have both glutamatergic AMPA and NMDA receptors (Inglis and Semba, 1996). Additionally, in 11 of these 27 neurons, glutamatergic antagonists blocked the evEPSC and unmasked a residual evIPSC. The evIPSCs were recorded at different holding potentials between -80 and -40 mV, revealing an average reversal potential of -62.7 ± 3.3 mV (n = 6), and were sensitive to the GABAA receptor antagonist BMI (20 µM; n = 4), suggesting mediation by GABAA receptor activation (Fig. 1B). In many areas of the CNS, adenosine inhibits both glutamatergic and GABAergic transmission (Shen and Johnson, 1997; Bagley et al., 1999; Oliet and Poulain, 1999). However, in hippocampus and septal nucleus, adenosine inhibits glutamatergic transmission but not GABAergic transmission (Yoon and Rothman, 1991; Hasuo et al., 1992). Therefore, we tested the effect of adenosine on glutamatergic evEPSCs and GABAergic evIPSCs. Pharmacologically isolated evEPSCs, recorded in the presence of BMI (20 µM), were reduced by adenosine (50 µM) by 42.35 ± 2.56% in 38 of 48 neurons tested (Fig. 1A). In the remaining 10 neurons, the evEPSCs were unaffected by adenosine (0.3 ± 2.04%). GABAergic evIPSCs, recorded during bath application of DNQX and APV (Vh = -45 to -40 mV), were also reduced by adenosine (50 µM) by 56.4 ± 7.1% in seven of nine neurons tested (Fig. 1B). Moreover, as shown in Figure 2, adenosine reduced the amplitude of the glutamatergic evEPSCs in a dose-dependent manner with maxresp = 77.3% and EC50 = 39.9 µM, which are similar to those previously found in the hippocampus and hypothalamus (Dunwiddie and Hoffer, 1980; Oliet and Poulain, 1999).



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  		Figure 1.   Adenosine modulates both glutamatergic- and GABAergic-evoked synaptic transmissions. A, Glutamatergic evEPSCs are inhibited by adenosine (AD). The evEPSC recorded at Vh = -60 mV is reversibly reduced by adenosine (50 µM) and blocked by perfusion of glutamatergic receptor antagonists DNQX (20 µM) and APV (100 µM). B, In another neuron, a GABAergic evIPSC is inhibited by adenosine. The evIPSC recorded in the presence of DNQX and APV (Vh = -40 mV) is reversibly reduced by adenosine and blocked by GABAA receptor antagonist BMI (20 µM). Each current trace is the average of 10 evEPSCs or evIPSCs.



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  		Figure 2.   Dose-response curve for adenosine inhibition of evEPSCs. The line represents the computer-generated fit to the data according to the equation: Y = minresp + {(maxresp - minresp)/(1 + 10 [(Log EC50 - Log AD) × H])}, with minresp = 0%, maxresp = 77.3%, EC50 = 39.9 µM, AD = adenosine concentration in micromolar, and Hill slope, H = 0.98. The numbers indicate the number of cells recorded.

Adenosine inhibits CNS neurons by activating a G-protein-activated inwardly rectifying K+ current (GIRK) (Trussell and Jackson, 1985), and in particular, this current is activated by adenosine in LDT neurons (Rainnie et al., 1994). Activation of the GIRK current might result in a conductance shunt of the evEPSC and hence contribute to the reduction of the evEPSC amplitude. Consequently, we examined 79 LDT neurons for the effects of adenosine on the isolated evEPSC and on activation of postsynaptic GIRK currents, the latter by measuring either activation of an outward current (Vh = -60 mV) (see Fig. 6A) or increase in the amplitude of currents obtained during a voltage ramp command from -100 to -35 mV, 10 mV/sec (see Fig. 6C1). Adenosine activated postsynaptic GIRK currents in 57% of the LDT neurons recorded. In 87% of these neurons, adenosine also induced inhibition of the evEPSC. Thus, in 13% of the neurons showing an adenosine-mediated increase in postsynaptic GIRK current, there was no adenosine-mediated inhibition of the evEPSC. Furthermore, reduction in the amplitude of the evEPSC was observed in 71% of the neurons that did not show adenosine postsynaptic effects, supporting the argument that the inhibition of the evEPSCs can be mediated independently of the postsynaptic effects.

Adenosine reduces the evoked EPSC amplitude without changing the postsynaptic response to exogenous glutamate application

In the absence of a GIRK-mediated shunt, the reduction in the evEPSC amplitude in the presence of adenosine could be caused by (1) changes in the amount of neurotransmitter released presynaptically or (2) changes in the responsiveness of postsynaptic glutamate receptors, or both. Adenosine inhibits glutamatergic transmission mainly through an activation of presynaptic adenosine receptors (for review, see Ribeiro, 1995; Brundege and Dunwiddie, 1997). However, recent studies have raised the possibility that adenosine can directly attenuate postsynaptic NMDA currents in hippocampal projection neurons and retinal bipolar cells (de Mendonca et al., 1995; Costenla et al., 1999). To determine whether the inhibitory effect of adenosine on the evEPSC in LDT neurons might result from a change in the postsynaptic response to glutamate, we compared the effect of adenosine on currents evoked by pressure-ejected glutamate (see Materials and Methods) with the effect of adenosine on evEPSC.

At the holding potential of -60 mV, transient (0.8-1.2 sec) application of glutamate (1 mM) evoked an inward current ranging between 0.95 and 2.2 nA in LDT neurons (n = 4). In four of four neurons tested, the glutamate-evoked currents were unaffected by adenosine (50 µM) (Fig. 3A), whereas the amplitude of the evEPSC was reduced by 47.9 ± 2.2% (Fig. 3B). Both the evEPSCs and the currents elicited by glutamate injection were sensitive to DNQX (20 µM) and APV (100 µM) applied in the bath (n = 3), indicating that both were mediated through activation of postsynaptic glutamatergic receptors (Fig. 3A,B). A direct inhibitory effect of adenosine on the postsynaptic glutamate receptors would seem unlikely, although a specific effect at electrotonically distant glutamate receptors cannot be excluded.



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  		Figure 3.   Modulation of glutamatergic transmission occurs independently of adenosine postsynaptic effects. A, Adenosine does not alter postsynaptic response to glutamate. Glutamate-evoked currents, elicited by exogenous glutamate (1 mM) pressure injected on the recording neuron (Vh = -60 mV), are unaffected by bath application of adenosine but are blocked by application of DNQX (20 µM) and APV (100 µM). The duration of each glutamate injection (1 sec) is indicated by the black bar above each current trace. B, In the same neuron, adenosine reversibly decreases evEPSC amplitude. Application of DNQX and APV eliminates the evEPSCs. C, Adenosine inhibition of the evEPSCs is independent of both presynaptic and postsynaptic GIRK activation. In another neuron, adenosine still reduces evEPSC amplitude in the presence of 2 mM BaCl2. Each evEPSC current trace is the average of 10 evEPSCs (Vh = -60 mV).

Adenosine reduces the frequency of mEPSPs

To further investigate the location of adenosine receptors, we next analyzed the action of adenosine on the amplitude and frequency of glutamatergic spontaneous mEPSCs. These spontaneous unitary events were recorded at a holding potential of -60 mV and in the presence of TTX (1 µM), which completely abolished evoked synaptic transmission. The frequency of mEPSCs ranged between 3.18 and 11.23 Hz under control conditions (n = 9), whereas the mean amplitude varied between 5 and 19.68 pA (191-674 events for each cell). Application of DNQX (20 µM) completely blocked the mEPSCs, suggesting mediation by glutamate (Fig. 4A). In five of the nine neurons recorded, occasional mIPSCs were observed that were sensitive to BMI (20 µM; n = 2), suggesting mediation by postsynaptic GABAA receptors. However, the frequency of mIPSCs was typically observed to be between 0.1 and 0.8 Hz under control conditions, which was too low for adequate analysis of amplitude and inter-event interval distribution. We therefore focused on adenosine modulatory effects of spontaneous mEPSCs.



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  		Figure 4.   Adenosine reduces glutamatergic mEPSC frequency without affecting mEPSC amplitude. A, Consecutive traces (2 sec each) recorded at Vh = -60 mV, showing typical mEPSC in control ACSF (left) and during the applications of adenosine (50 µM; middle) and DNQX (20 µM; right). Adenosine produces a decrease in frequency of mEPSC, and DNQX blocks the mEPSCs. B, C, Cumulative distribution plots of mEPSC amplitude and inter-event interval before (thick line) and during (thin line) adenosine application for the experiment shown in A. The cumulative plots show that adenosine does not induce a significant variation of the mEPSC amplitude (p > 0.1, K-S test; events analyzed = 685 in control and 232 with adenosine), whereas it does induce a shift toward the right in the distribution of inter-event intervals (p < 0.001, K-S test), indicating a decrease in mEPSC frequency. The mean mEPSC amplitude and frequency data pooled from nine neurons are represented in the two histograms (B, C, insets). Adenosine reduces mean mEPSC frequency (6.18 ± 0.79 Hz in control; 3.21 ± 0.34 Hz in adenosine; p = 0.0015, paired t test) without significant effect on the mean mEPSC amplitude (10.25 ± 1.44 pA in control; 9.35 ± 1.15 pA with adenosine; p = 0.066, paired t test).

When adenosine (50 µM) was added to the bathing solution, the spontaneous mEPSC activity was reduced in all neurons recorded (n = 9) (Fig. 4A). We examined the amplitude and inter-event interval distributions of the spontaneous mEPSCs. The results of a representative neuron are shown in Figure 4, B and C. No significant difference was found between the amplitude distribution obtained under control conditions and that obtained in the presence of adenosine (p > 0.1, K-S test; n = 9). Moreover, the amplitude of the spontaneous mEPSC was unaffected in five of the nine recorded neurons in which adenosine activated a postsynaptic GIRK conductance (17.95 ± 12 nS). In contrast, application of adenosine did affect the cumulative inter-event interval distribution of the mEPSC, producing a significant rightward shift of the curve (p < 0.01, K-S test; n = 9), reflecting a decrease of mEPSC frequency. Pooled data from nine recorded neurons are represented in Figure 4, B and C (insets), and show that adenosine decreases the mean mEPSC frequency (6.18 ± 0.79 Hz, in control; 3.21 ± 0.34 Hz, with adenosine; p = 0.0015, paired t test) in the absence of a significant change in the mean mEPSC amplitude (10.25 ± 1.44 pA, in control; 9.35 ± 1.1 pA, with adenosine; p = 0.066 paired t test).

Modifications of miniature synaptic current frequency, in the absence of changes in amplitude distribution, most likely reflect a change in neurotransmitter release probability (Redman, 1990). Our findings are therefore consistent with a presynaptic location for the adenosine receptors, the activation of which results in an inhibition of glutamate release. Because the amplitude distributions of the mEPSCs were not significantly affected, it is unlikely that postsynaptic glutamate receptors are directly modulated by adenosine.

Does activation of GIRK current mediate the presynaptic inhibition?

One of the possible mechanisms by which adenosine can inhibit evoked glutamatergic transmission through presynaptic A1 receptor activation is by activating the same GIRK current that adenosine activates postsynaptically, thus shunting the presynaptic action potential and decreasing the voltage-dependent Ca2+ entry (for review, see Brundege and Dunwiddie, 1997). To determine whether the inhibitory effect of adenosine on the evoked EPSC was mediated by presynaptic activation of GIRK, we tested the effect of adenosine in the presence of 2 mM BaCl2, which is known to completely block the GIRK conductance activated by adenosine (Gerber et al., 1989; Birnstiel et al., 1992). Barium did not block adenosine (50 µM) inhibition of the evEPSC in any neurons tested (Fig. 3C). In the presence of BaCl2, adenosine still reduced evEPSC amplitude by 53.8 ± 7.3% (n = 6), compared with a reduction of 42.35 ± 2.56% by adenosine (50 µM; n = 38) in control conditions. The activation of presynaptic GIRK is therefore unlikely to contribute to the adenosine-mediated inhibition of the evEPSC.

Adenosine inhibits evoked excitatory inputs via activation of A1 receptors

In the CNS, the inhibitory effect of adenosine on cellular and synaptic activity appears to be primarily mediated by A1 receptors (for review, see Greene and Haas, 1991; Brundege and Dunwiddie, 1997). Adenosine reduces the activity of LDT neurons through direct activation of postsynaptic A1 receptors (Rainnie et al., 1994). Here we have shown an additional indirect inhibition by reduction of excitatory inputs; however, the subtype of the receptor involved in this effect was unknown.

We studied the effects of the A1 receptor agonist CHA (100 nM) for the selective activation of A1 receptors (EC50 = 1-2 nM at A1 receptors; EC50 = 450-1000 nM at A2 receptors) (Bruns et al., 1986). The effects of application of CHA on evEPSCs were identical in character to those of adenosine (n = 9) (Fig. 5A,E). In six of these neurons tested with CHA, the evEPSC amplitude was reduced by 59.8 ± 6.3% and by 48.8 ± 2.7% with adenosine. In the remaining three neurons, the evEPSCs were unaffected by either CHA or adenosine.



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  		Figure 5.   Exogenous and endogenous adenosine inhibit the evEPSCs through activation of A1 receptors. A, Application of the A1 receptor agonist CHA (100 nM) reduces the evEPSC amplitude. B, The evEPSC inhibition mediated by 50 µM adenosine (AD) is removed by the A1 receptor antagonist CPT (200 nM). C, A neuron sensitive to adenosine (left) does not respond to the A2 agonist DPMA (10 nM) but does respond to further application of CHA (right). D, Application of CPT alone induces an increase in the evEPSC amplitude. E, Summary of the effects on the evEPSC. The histogram shows the mean ± SE of the evEPSC peak amplitude during applications of CHA, DPMA, and CPT expressed as a percentage of their respective evEPSC amplitude in control. Only adenosine-sensitive cells were considered. The numbers indicate the number of cells recorded: *p < 0.01 versus evEPSC amplitude under control conditions; paired t test.

In a second set of experiments, we antagonized adenosine inhibition of the evEPSCs with the A1 receptor antagonist CPT (Ki = 10.9 nM at A1 receptors; Ki = 1440 nM at A2 receptors) (Bruns, 1981). Application of CPT (200 nM; n = 10) completely antagonized the effects of adenosine (50 µM), which elicited a reduction of evEPSC by 48.7 ± 6.9%. During the course of these experiments it was noted that compared with control conditions, CPT caused a significant increase in the evEPSC amplitude (11.7 ± 6.93%; p = 0.039, paired t test), consistent with removal of a tonic inhibition by endogenous adenosine (Fig. 5B). Taken together, these results suggest that adenosine inhibits evoked excitatory transmission in the LDT through activation of presynaptic A1 receptors. However, the increase in evEPSC amplitude observed in the presence of the A1 receptor antagonist might be caused, at least in part, by the activation of additional adenosine receptor subtypes.

Are adenosine A2 receptors involved in the modulation of the evoked EPSC?

Although activation of the A1 receptors induces inhibitory effects on cellular and synaptic activity, activation of A2 receptors induces presynaptic and postsynaptic excitatory responses (Palmer and Stiles, 1995). In several areas of the CNS, A1 and A2 receptors are coexpressed, for instance, in the striatum (Brown et al., 1990) and in the hippocampus (Li and Henry, 1998). Enhancement of neurotransmitter release by adenosine is evident only when A1 receptors are blocked. On the basis of these observations, the increased evEPSC amplitude that we observed with CPT in the presence of adenosine (Fig. 5B) might have resulted from the coactivation of A2 receptors.

We examined this possibility by testing the activation of A2 receptors with the selective A2 receptor agonist, DPMA. To selectively activate the A2 rather than the A1 receptors, we used DMPA at a concentration of 10 nM (Ki = 142 nM at A1 receptors; Ki = 4.4 nM at A2 receptors) (Bridges et al., 1988), a concentration that elicited an A2-dependent enhancement of the evEPSC amplitude in hippocampal slices in vitro (Kessey and Mogul, 1998). Applications of DPMA did not alter the amplitude or the duration of the evEPSC (98.9 ± 1.25% of the control amplitude; n = 6) (Fig. 5C,E), nor did it affect postsynaptic membrane potential or resistance. However, in the same cells, adenosine inhibited the evEPSC by 41.8 ± 4.4%. These findings suggest that A2 receptor activation does not mediate adenosine effects on evEPSC or excitability of LDT neurons.

Excitatory synaptic transmission is under inhibitory control by endogenous adenosine

Application of adenosine A1 receptor antagonists induces excitatory presynaptic and postsynaptic responses compatible with a removal of the inhibitory tone of endogenous adenosine (Dunwiddie et al., 1981; Greene et al., 1985; Haas and Greene, 1988). In the LDT, application of CPT induces an increase in neuronal activity as well as an increase in the amplitude of hyperpolarization-activated current (Ih), which is reduced by adenosine (Rainnie et al., 1994). To test the possibility that glutamatergic transmission could also be under tonic inhibition by endogenous adenosine, we measured the effects of CPT on the glutamatergic evEPSC. In those neurons that were adenosine sensitive, CPT (200 nM) alone induced an increase in the evEPSC amplitude by 20 ± 5.9% (n = 10) (Fig. 5D,E). In addition, the holding currents of five of these neurons were reduced through the application of CPT by 24.8 ± 11.5 pA, which is similar to the reduction previously reported for LDT neurons (Rainnie et al., 1994). These data confirm that in the LDT, the endogenous, extracellular, adenosine concentration is sufficiently high to induce a physiologically measurable inhibition of synaptic glutamate release. In the hypothalamic supraoptic nucleus, CPT only potentiates glutamatergic evEPSCs after train stimulation of 200 sec duration (Oliet and Poulain, 1999). In contrast, in the LDT, electrophysiologically significant basal release of adenosine is appreciable.

Adenosine kinase inhibitor 5-iodotubercidin increases endogenous adenosine levels

Adenosine is primarily formed from the breakdown of ATP within cells and is transported across the cell membrane by equilibrative transporters (Geiger and Fyda, 1991; Thorn and Jarvis, 1996). Thus, any increase in the intracellular adenosine concentration is likely to be reflected as an increase of adenosine in the extracellular space (Brundege and Dunwiddie, 1996). Intracellular adenosine metabolism occurs through phosphorylation by adenosine kinase and deamination by adenosine deaminase. Blockers of these two enzymes can induce an increase in the intracellular adenosine level and consequently extracellular adenosine levels (Brundege and Dunwiddie, 1997). Inhibitors of adenosine kinase, more than inhibitors of adenosine deaminase, increase extracellular adenosine levels in hippocampal, spinal cord, and cortical slices under control conditions (Pak et al., 1994; Lloyd and Fredholm, 1995; Golembiowska et al., 1996; White, 1996), consistent with the higher affinity of adenosine kinase for adenosine (Arch and Newsholme, 1978). The adenosine kinase inhibitor ITU can decrease neuronal activity in hippocampal and cortical slices by increasing extracellular adenosine levels (Pak et al., 1994; White, 1996). Consequently, we examined the effects of ITU in the LDT. Neurons sensitive to adenosine responded to ITU application (n = 9) in a manner indistinguishable from adenosine. Application of ITU (10 µM) induced an increase in the membrane conductance (Fig. 6A) shown by both (1) activation of an outward current (Vh = -60 mV) and (2) an increase in the amplitude of the current evoked by hyperpolarizing voltage step commands to -100 mV. The response to ITU appeared in 3-6 min, whereas its recovery was only observed after >45 min of drug washout. However, ITU effects were rapidly blocked when the A1 receptor antagonist CPT (200 nM) was applied (Fig. 6A). Three neurons that were insensitive to adenosine were also insensitive to ITU.



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  		Figure 6.   ITU induces postsynaptic activation of an inwardly rectifying K+ current and inhibition of the evoked glutamatergic transmission, similar to adenosine. A, Currents recorded at Vh = -60 mV. Downward deflections resulted from three different voltage protocol commands indicated by the numbers: 1, voltage pulses to -100 mV (200 msec; 0.2 Hz); 2, voltage ramps from -100 to -35 mV (10 mV/sec); 3, stimulation with a bipolar stimulating electrode (0.3 Hz). All protocols are performed in control ACSF and during applications of adenosine (AD; 50 µM), ITU (10 µM), and CPT (200 nM), indicated by black bars above the current traces. Both adenosine and ITU induce increases in the membrane conductance. The outward current elicited by ITU was rapidly blocked by application of CPT. B, evEPSCs recorded during control condition, adenosine, and ITU applications. The evEPSCs are inhibited by both adenosine and ITU. Each current trace is the average of 10 evEPSCs. C1, C2, Recordings from another neuron, obtained during the voltage-ramp protocols; each current trace displayed is the average of three consecutive voltage ramps. Adenosine (C1) and ITU (C2) increase the membrane slope conductance at all membrane potentials between -100 and -35 mV. The effect of ITU is antagonized by CPT (C2). D, Current-voltage relationships of adenosine (IAD) and ITU (IITU) evoked currents, calculated by digital subtraction (IAD = AD - CON) and (IITU 61 ITU - CON) of the currents displayed in C1 and C2. IAD and IITU show the same reversal potential (-81 mV) and the same voltage-dependent slope conductance, consistent with adenosine and ITU-mediated inwardly rectifying K+ current activations. E, ITU chord conductance (GITU) as a function of membrane potential (Em). GITU is obtained from the values of IITU shown in D (GITU = IITU/(Em - Ereversal) and is fit to the Boltzmann equation (line) GITU = GITU(min) + (GITU(max) - GITU(min))/{1 + exp [(Em - E1/2)/k]}, with GITU(max) = 56 nS, GITU(min) = 19.6 nS, E1/2 = -87 mV, and k = 9.7 mV.

To investigate whether the responses to adenosine and ITU were mediated by activation of the same GIRK, we compared the voltage sensitivity and reversal potential of the current evoked by adenosine (IAD) with those evoked by ITU (IITU). Currents obtained during a voltage ramp command from -100 to -35 mV (10 mV/sec) were recorded before and during adenosine application (Fig. 6C1), and before and during ITU application (Fig. 6C2). These recordings were used to obtain current-voltage relationships for IAD and IITU by digitally subtracting the currents recorded under control conditions from those recorded during drug applications (Fig. 6D). Current-voltage relationships obtained from five neurons showed that IITU had the same rectification properties and an identical reversal potential as IAD (EITU = -82.04 ± 9.7 mV; EAD = -82.6 ± 3.87 mV) compared with adenosine-mediated GIRK (Rainnie et al., 1994). Moreover, IITU was blocked by CPT (200 nM; n = 4) (Fig. 6C2). The ITU chord conductance (GITU) as a function of membrane potential (Em), shown in Figure 6E, was derived from the IITU voltage relationship. A curve constrained by the Boltzmann equation (Fig. 6, legend) with half-activated potential, E1/2 = -87 mV, and a slope factor, k = 9.7 mV, was then fit to the data. These values were similar to those reported in LDT neurons for the adenosine chord conductance (Rainnie et al., 1994).

ITU inhibits the evEPSC

We next examined the effect of ITU on the evEPSC. Similar to adenosine, application of ITU inhibited evoked excitatory transmission (Figs. 6B, 7). evEPSCs were recorded from eight neurons, and the response to exogenous adenosine and ITU application was examined. In six neurons, adenosine reduced the evEPSC by 51.1 ± 3.23%, and ITU reduced the evEPSC by 49 ± 4%. In the remaining two neurons, the evEPSCs were insensitive to both adenosine and ITU application. From the dose-response relationship characterizing the effect of exogenous adenosine on the evEPSC amplitude (Fig. 2), we can estimate that the inhibitory effect of ITU on the evEPSC is comparable with an application of exogenous adenosine of ~50-100 µM. In addition, the evEPSC inhibition by ITU could be completely antagonized by the A1 receptor antagonist CPT (200 nM; n = 3) (Fig. 7A) and by application of the enzyme adenosine deaminase (0.8 IU/ml; n = 2) (Fig. 7B).



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  		Figure 7.   The inhibitory effect of ITU on the evEPSCs can be blocked by A1 receptor antagonist CPT or by adenosine deaminase. A, CPT (200 nM) antagonized the inhibition of the evEPSC by ITU. B, In another neuron, adenosine deaminase (ADA) (0.8 IU/ml) partially removed the inhibitory effect of ITU. Each current trace is the average of 10 evEPSCs (Vh = -60 mV).

These data, taken together, show that in an in vitro preparation, LDT neurons are tonically inhibited by extracellular endogenous adenosine and that the level of endogenous adenosine can be dramatically altered by manipulating the adenosine kinase responsible for adenosine metabolism.


    DISCUSSION
TOP
ABSTRACT
INTRODUCTION
MATERIALS AND METHODS
RESULTS
DISCUSSION
REFERENCES

In summary, adenosine reduces both glutamate- and GABA-mediated evEPSCs and evIPSCs recorded from neurons of the LDT nucleus. Moreover, by focusing on the excitatory input, we found that adenosine directly inhibits glutamatergic transmission by activating presynaptic adenosine A1 receptors. In addition, a reduction of adenosine catabolism by an inhibition of adenosine kinase results in an activation of presynaptic and postsynaptic A1 receptors comparable, in both character and degree, with activation by exogenous adenosine (50-100 µM).

Presynaptic inhibition

Evidence for a presynaptic locus of the adenosine-mediated inhibition of the glutamate transmission is based on several independent results. First, the adenosine-mediated inhibition of glutamatergic evEPSCs was not associated with alterations in the postsynaptic sensitivity of glutamate receptors. Second, adenosine reduced the frequency, but not amplitude, of spontaneous glutamatergic mEPSCs. Third, the presynaptic effect of adenosine could occur independently of a postsynaptic adenosine effect.

The presynaptic mechanism through which adenosine inhibits the glutamatergic release remains to be determined. However, the inhibition of the evEPSC by adenosine was maintained in the presence of 2 mM BaCl2, which is known to block the postsynaptic GIRK conductance activated by adenosine (Gerber et al., 1989; Birnstiel et al., 1992). It is probable, therefore, that adenosine does not inhibit glutamatergic evEPSCs by activating a GIRK conductance in the presynaptic terminal. This result is consistent with the literature, which does not support a presynaptic GIRK activation as a primary mechanism by which adenosine inhibits neurotransmitter release (Dunwiddie and Miller, 1993; Brundege and Dunwiddie, 1997). It is more likely that the inhibition of excitatory transmission in the LDT nucleus occurs via the modulation of presynaptic calcium channels. Adenosine is known to (1) inhibit voltage-dependent Ca2+ channels (Dolphin et al., 1986; Scholz and Miller, 1991; Mogul et al., 1994) and (2) reduce the evEPSC amplitude by inhibiting the presynaptic calcium fluxes in hippocampus and striatum (Wu and Saggau, 1994; Ambrosio et al., 1997). Alternatively, or in combination, adenosine may also act presynaptically to inhibit glutamate release at a point downstream from Ca2+ entry, as shown in the hippocampus where adenosine still reduces spontaneous synaptic activity when Ca2+ entry is blocked (Scanziani et al., 1992; Scholz and Miller, 1992). Recently, a reduction of glutamatergic and GABAergic transmission has been shown after presynaptic inhibition of adenylate cyclase-protein kinase A cascade (Tzounopoulos et al., 1998; Bagley et al., 1999). Activation of A1 receptors also inhibits adenylate cyclase activity, which mediates the reduction of presynaptic GABA release in the periacqueductal gray neurons (Bagley et al., 1999) and may contribute to the presynaptic inhibition of glutamate release observed in the LDT nucleus.

Endogenous adenosine

We provide direct evidence for the presence of a basal endogenous adenosine level in the LDT nucleus that can tonically activate adenosine A1 receptors. Hence, application of CPT potentiated the stimulus-evoked glutamatergic EPSC. This is compatible with a removal of an endogenous inhibitory adenosine tone on the glutamatergic input to the LDT and may represent the presynaptic mechanism contributing to the CPT-induced increase in network excitability observed with extracellular recording in the LDT (Rainnie et al., 1994).

It has been reported that intracellular catabolism of adenosine may directly affect the extracellular concentration of adenosine through the adenosine equilibrative facilitated transporter system (Geiger and Fyda 1991; Pak et al., 1994; Brundege and Dunwiddie, 1996, 1997). Thus, if catabolism is slowed, then an increase in intracellular adenosine may be reflected by an increase in extracellular adenosine. Of the enzymes that catabolize adenosine, adenosine kinase has the highest affinity (Arch and Newsholme, 1978) and thus, under baseline conditions, is most likely to affect the rate of adenosine catabolism (Lloyd and Fredholm, 1995). The present study suggests that when adenosine kinase activity is reduced with ITU, extracellular adenosine levels dramatically increase to give an adenosine-mediated electrophysiological response approximately equal to application of exogenous adenosine at a concentration between 50 and 100 µM. In addition to being a potent adenosine kinase blocker, ITU is also known to inhibit the equilibrative adenosine transporter (Davies and Cook, 1995). Nevertheless, it is unlikely that ITU induces extracellular adenosine accumulation by blocking adenosine reuptake. First, prolonged application of the adenosine transport blockers NBTI (5 µM, n = 4; 30 µM, n = 6) and dipyridamole (5 µM; n = 4) does not induce any presynaptic or postsynaptic effects in any of the adenosine-sensitive LDT neurons tested. Second, presynaptic and postsynaptic effects of ITU occur in the first 10 min of its application, whereas adenosine transport inhibitors cause a slow and gradual accumulation of extracellular adenosine (Brundege and Dunwiddie, 1997; Dunwiddie and Diao, 2000). Therefore, it is probable that blockade of adenosine kinase is the major mechanism by which ITU increased extracellular adenosine levels. Moreover, we cannot exclude the possibility that the final extracellular adenosine accumulation, resulting from the adenosine kinase blockade by ITU, may actually be blunted by the additional inhibitory action of ITU on the adenosine transporters.

Adenosine inhibits glutamatergic input to cholinergic LDT neurons

The LDT nucleus contains a heterogeneous neuronal population of cholinergic and noncholinergic neurons (Kamondi et al., 1992), part of which has been identified as GABAergic (Ford et al., 1995). It has been reported that 60% of the LDT neurons affected postsynaptically by adenosine were cholinergic (Rainnie et al., 1994). We show that in 87% of postsynaptically responding neurons, adenosine also reduces evoked glutamatergic transmission, suggesting that cholinergic LDT neurons can be inhibited by activation of both presynaptic and postsynaptic adenosine receptors. In addition, results from the recording of spontaneous mEPSCs, which better represent the total afferent input conserved in the slice, show that adenosine reduces spontaneous glutamatergic mEPSC frequency in all LDT neurons.

Therefore, adenosine presynaptic responses appear to be more a function of the input rather than of the postsynaptic cell type. We can conclude that the glutamatergic afferents to the LDT neurons do not all have presynaptic adenosine receptors, but virtually all LDT neurons appear to receive at least a portion of glutamatergic projection that is sensitive to adenosine.

Functional role of adenosine modulation of the synaptic input in the LDT nucleus

Exogenous adenosine reduces spontaneous cell firing of action potentials in 100% of the cells recorded extracellularly in the slice (Rainnie et al., 1994). However, because adenosine inhibits only 80% postsynaptically (Rainnie et al., 1994), the remaining 20% of neurons were inhibited solely by a presynaptic action on the glutamatergic input. Thus, it is likely that with respect to isolated spontaneous local circuit inputs conserved in the slice, adenosine presynaptic effects are predominately disfacilitatory, and the overall effect of presynaptic and postsynaptic adenosine action is inhibitory. The caveat remains that in vivo, the state-dependent balance of disfacillitation and disinhibition is ultimately determined by the state-dependent presynaptic activity of the adenosine-sensitive excitatory and inhibitory inputs.

Recent studies in the literature show the presence of a large number of GABAergic neurons in the LDT nucleus; the majority of them give rise to dense local GABAergic innervations (Ford et al., 1995), and analysis of c-Fos expression suggests that they may be active during REM sleep (Maloney et al., 1999). This local GABAergic circuit is a most likely target of our stimulation protocol in vitro. If this is the case, adenosine is likely to inhibit the output of these GABAergic REM-on neurons.

If an adenosine-sensitive, wake-dependent, excitatory activity predominates in vivo, then our data are consistent with a combined presynaptic and postsynaptic inhibitory adenosine tone during waking. This is consistent with the hypothesis that a localized increase in extracellular adenosine in one or both of the cholinergic arousal centers (the LDT/PPT and the BF) (Portas et al., 1997) facilitates the transition from waking to sleep by inhibition of the activity of these neurons (Rainnie et al., 1994). Because a localized increase in extracellular adenosine in the LDT is sufficient to facilitate the transition from waking to sleep (Portas et al., 1997), the control of the extracellular adenosine concentration may be important in the control of the behavioral state. Indeed, sleep deprivation that increases the probability of making the transition from wake to sleep is associated with an increase in endogenous extracellular adenosine in the BF cholinergic arousal center (Porkka-Heiskanen et al., 1997).

We have demonstrated that by reducing the intracellular catabolism of adenosine with an adenosine kinase inhibitor we can significantly increase extracellular levels of adenosine. If this occurs in the cholinergic arousal centers in vivo, then the likelihood of a behavioral state transition from waking to sleep would be increased. Because adenosine catabolism by adenosine kinase may be altered by changes in the intracellular concentrations of adenosine, AMP, ADP, or ATP (Hawkins and Bagnara, 1987; Mimouni et al., 1994; Pelicano et al., 1997), the localized metabolic state of the cholinergic arousal centers could affect the extracellular adenosine concentration and, accordingly, the behavioral state.


    FOOTNOTES

Received July 21, 2000; revised Nov. 6, 2000; accepted Nov. 24, 2000.

This work was supported by a Merit Award from The Department of Veterans Affairs to R.W.G., and by Specialized Center of Research (SCOR) Grant NHLBI-HL60292. E.A. was supported by the University of Milan, Italy and by SCOR Grant NHLBI-HL60292. We also thank Dr. M. R. Palmer for help in the preparation of this manuscript.

Correspondence should be addressed to Dr. Robert W. Greene, Harvard Medical School and Veterans Administration Medical Center, Laboratory of Neuroscience, 151-C, 940 Belmont Street, Brockton, MA 02401. E-mail: robert_greene{at}HMS.harvard.edu.


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