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The Journal of Neuroscience, February 15, 2001, 21(4):1148-1159

Muscarinic Regulation of Dendritic and Axonal Outputs of Rat Thalamic Interneurons: A New Cellular Mechanism for Uncoupling Distal Dendrites

J. Julius Zhu^{1, 2} and Paul Heggelund^{1, 3}

¹ Department of Cell Physiology, Max-Planck-Institute for Medizinische Forschung, Heidelberg D-69120, Germany, ² Cold Spring Harbor Laboratory, Cold Spring Harbor, New York 11724, and ³ Department of Physiology, University of Oslo, N-0317 Oslo, Norway

	ABSTRACT

TOP ABSTRACT INTRODUCTION MATERIALS AND METHODS RESULTS DISCUSSION REFERENCES

Inhibition is crucial for sharpening the sensory information relayed through the thalamus. To understand how the interneuron-mediated inhibition in the thalamus is regulated, we studied the muscarinic effects on interneurons in the lateral posterior nucleus and lateral geniculate nucleus of the thalamus. Here, we report that activation of muscarinic receptors switched the firing pattern in thalamic interneurons from bursting to tonic. Although neuromodulators switch the firing mode in several other types of neurons by altering their membrane potential, we found that activation of muscarinic subtype 2 receptors switched the fire mode in thalamic interneurons by selectively decreasing their input resistance. This is attributable to the muscarinic enhancement of a hyperpolarizing potassium conductance and two depolarizing cation conductances. The decrease in input resistance appeared to electrotonically uncouple the distal dendrites of thalamic interneurons, which effectively changed the inhibition pattern in thalamocortical cells. These results suggest a novel cellular mechanism for the cholinergic transformation of long-range, slow dendrite- and axon-originated inhibition into short-range, fast dendrite-originated inhibition in the thalamus observed in vivo. It is concluded that the electrotonic properties of the dendritic compartments of thalamic interneurons can be dynamically regulated by muscarinic activity.

Key words: rat; thalamus; interneurons; inhibition; cholinergic receptors; cortex

	INTRODUCTION

TOP ABSTRACT INTRODUCTION MATERIALS AND METHODS RESULTS DISCUSSION REFERENCES

The thalamus relays sensory information from the periphery to cortex in a state-dependent manner (Sherman and Guillery, 1996). In an awake, attentive state, thalamic neurons fire tonic action potentials, and the transmission of sensory inputs is relatively faithful. During slow-wave sleep, thalamic cells resume a rhythmic burst-firing pattern, which allows only salient sensory inputs to be amplified and transmitted. Thalamic inhibition, provided mainly by GABAergic cells in the reticular nucleus and local interneurons in the dorsal nuclei, modulates the sensory information relayed through thalamocortical cells. During wakefulness, fast, short-duration IPSPs, ideal for maintaining high signal resolution, dominate, whereas in slow-wave sleep, slow, long-duration IPSPs, perfect for promoting oscillation and burst activity, prevail in the thalamus (Steriade et al., 1996).

Neuromodulators [e.g., acetylcholine (ACh)], which are released in a state-dependent manner, regulate the firing patterns in thalamic cells (Steriade et al., 1997). Activation of the cholinergic input from the brainstem, which occurs during arousal, affects sensory transmission in the thalamus (Sillito et al., 1983; Francesconi et al., 1988; Hartveit et al., 1993; Hartveit and Heggelund, 1994, 1995; Uhlrich et al., 1995). In particular, inhibitory sharpening of sensory information is dramatically altered during the activation (Ahlsén et al., 1984; Hu et al., 1989; Hartveit and Heggelund, 1993; Murphy et al., 1994). In vitro studies have shown that various neuromodulators change the membrane potential of reticular and thalamocortical cells (Steriade et al., 1997), which switches their firing patterns by inactivating or deinactivating a low-threshold calcium current crucial for burst firing (Huguenard, 1996). This affects the sensory signals transmitted through the thalamus, because the forms of inhibition produced by reticular cells onto thalamocortical cells depend on the firing pattern of reticular cells (Huguenard and Prince, 1994; Destexhe and Sejnowski, 1995; Kim et al., 1997).

Compared with reticular cells, local interneurons appear to play a more important role in sharpening sensory information in the thalamus because they can produce larger varieties of inhibition on thalamocortical cells. These cells release GABA not only through conventional axonal terminals but also via the dendritic triadic structures, which are postsynaptic to the sensory afferents but presynaptic to the dendrites of thalamocortical cells and other interneurons (Ralston, 1971; Zhu and Lo, 1999; Cox and Sherman, 2000). The two release sites allow interneurons to generate multiple forms of inhibition in thalamocortical cells (Paré et al., 1991; Curró Dossi et al., 1992). In addition, interneurons have two or three prolonged dendrites and one locally branched axon (Zhu and Lo, 1999), which may contribute to the long- and short-range inhibitions observed in the thalamus in vivo (Eysel et al., 1986). How the distinct types of interneuron-mediated inhibition are differentially regulated, particularly during different behavioral states, is largely unknown. We examined muscarinic effects on the firing patterns (i.e., bursting vs tonic) (Zhu et al., 1999a) and outputs of interneurons. We found that the predominate muscarinic effect was a decrease in input resistance in interneurons, which effectively switched the interneuron-mediated inhibition from a long-range, slow one to a local, fast one.

	MATERIALS AND METHODS

TOP ABSTRACT INTRODUCTION MATERIALS AND METHODS RESULTS DISCUSSION REFERENCES

Experiments were performed in thalamic slices from Wistar rats that were postnatal 27-56 d old (100-280 gm). No differences in electrophysiological properties of interneurons was found over this age range (Zhu et al., 1999c). The rats were deeply anesthetized by halothane and decapitated. The brain was then quickly removed and placed into cold (1-4°C) physiological solution containing (in mM): NaCl 125, KCl 2.5, NaH₂PO₄ 1.25, NaHCO₃ 25, MgCl₂ 1, dextrose 25, CaCl₂ 2, at pH 7.4. This surgical operation, which appeared to be crucial for obtaining healthy slice tissues from the adult animals, was accomplished as fast as possible (within 15 sec). Slices containing lateral geniculate nucleus (LGN), each 300-500 µm thick, were then cut from the tissue blocks in cold (0-4°C), oxygenated physiological solution, using a microslicer (Campden Instrument). The advance speed of the microslicer was adjusted to be as slow as possible during the slicing. These slices were then kept in warm (37.0 ± 0.5°C), oxygenated physiological solution for ~1 hr before recording. During the recordings, slices were submerged in a Plexiglas chamber and stabilized using a fine nylon net attached to a platinum ring. The chamber was perfused with warmed, oxygenated physiological solution, and the half-time for the bath solution exchange was ~6 sec. The temperature of the bath solution in the chamber was kept at 35.0 ± 0.5°C. Agonists and antagonists were typically applied with the bath solution at 7-15 min intervals. To examine the sustained muscarinic effects, 1 mM acetyl--methcholine (Mch) was continuously perfused with the physiological solution. Intrinsic and synaptic properties were studied typically 2-4 min after the beginning of perfusion to ensure that the activation of muscarinic receptors reached the steady state. Brief (4-8 sec) application of Mch was achieved by switching between two perfusing tubings containing either nothing or 1 mM Mch in the physiological solution. Approximately 4-7 min were allowed to wash in and wash out the antagonists. TTX (4 µM) was typically included in the bath solution after interneurons or thalamocortical cells were physiologically identified, except when the firing patterns or synaptic responses were examined.

Whole-cell recordings from thalamic interneurons and thalamocortical cells were made as described previously (Zhu and Lo, 1999). Patch electrodes were made from borosilicate tubing, and their resistances were 5-9 M with our intracellular solution containing (in mM): potassium gluconate 115, HEPES 10, MgATP 2, Na₂ATP 2, GTP 0.3, and KCl 20 at pH 7.3. Liquid junction potential (9 mV) was subtracted from all membrane potentials. We found little washout effect on the intracellular Ca²⁺ concentration with this intracellular solution, as indicated by no change in Ca²⁺-dependent muscarinic depolarization during the prolonged recordings (see Fig. 3C,D) (cf. Helmchen et al., 1996). Whole-cell recordings were made with up to two Axoclamp-2B or Axopatch-1D amplifiers (Axon Instruments). Single-electrode voltage-clamp mode was chosen to record current responses. To obtain current versus voltage (I-V) plots, the cells were initially held at 70 mV. Then, after 2 sec of hyperpolarization of 130 to 125 mV, the membrane potential was continuously ramped to 55 to 40 mV over a period of 5-10 sec.

Synaptic responses were examined using both acute and organotypic culture thalamic slices. Only slices that contain no thalamic reticularnucleus were used (sliced from bregma 5.0 to 4.2). As described previously (Zhu et al., 2000), culture slices were incubated in the culture medium containing (in percentage or mM): MEM 79%, horse serum 20%, L-glutamine 3, glucose 18.4, NaHCO₃ 5, CaCl₂ 2.26, MgSO₄ 2.81, HEPES 30, ascorbic acid 0.07, insulin 0.00017, at pH 7.28. Cortical slices from the same animals were co-cultured with the thalamic explants to increase the survival rate of thalamic cells (cf. Bolz et al., 1992; Sieg et al., 1998). The recordings were performed at ~36-72 hr after culture, which allowed only degeneration of the severed axons from reticular cells (Ohara et al., 1980) but not large-scale reorganization of the local circuitry (cf. McKinney et al., 1999). The survived thalamic interneurons and thalamocortical cells from culture slices appeared to be healthy, and they had the same resting membrane potential (acute: 66.5 ± 0.8 mV, n = 34; culture: 62.5 ± 1.8 mV, n = 4; t test, p = 0.12 for interneurons; acute: 68.3 ± 0.7 mV, n = 30; culture: 66.4 ± 0.9 mV, n = 17; t test, p = 0.14 for thalamocortical cells), input resistance (acute: 535 ± 24 M, n = 34; culture: 578 ± 50 M, n = 4; t test, p = 0.56 for interneurons; acute: 98 ± 6 M, n = 30; culture: 108 ± 9 M, n = 17; t test, p = 0.49 for thalamocortical cells), and time constant (acute: 93.8 ± 3.3 msec, n = 34; culture: 92.0 ± 3.8 msec, n = 4; t test, p = 0.35 for interneurons; acute: 17.1 ± 0.8 msec, n = 30; culture: 15.1 ± 0.8 msec, n = 17; t test, p = 0.09 for thalamocortical cells) as those recorded from acute slices. Thalamic interneurons were directly activated by one or two bipolar electrodes (FHC Inc., Bowdoinham, ME) with single voltage pulses (200 µsec, 0.4-7 V, 0.25 Hz). NBQX (5 µM) and DL-AP5 (100 µM) were included in the bath solution to block excitatory synaptic transmission. Stimulating electrodes were placed ~200 µm away from the recorded interneurons and ~200-500 µm away from the recorded thalamocortical cells to induce direct excitation in interneurons, proximal and/or distal interneuron-mediated inhibitions in thalamocortical cells. The similar stimulation intensity was used for activating the proximal and distal populations of interneurons. Synaptic responses were averaged over 10-50 trials. All results are reported as mean ± SEM. Statistical differences of the means were determined using paired t test unless stated otherwise. The level of significance was set at p < 0.05.

To recover cell morphology, biocytin (0.25%) was included in the intracellular solution. After recordings, slices were fixed by immersion in 0.1 M phosphate buffer containing 4% paraformaldehyde, resected into 150- to 250-µm-thick sections, and histologically reacted for biocytin to recover the cell morphology. Cells were subsequently drawn under 100× objective with the aid of a computerized reconstruction system (Neurolucida 3.18) or a camera lucida system. Chemicals were purchased from Sigma-RBI (St. Louis, MO).

	RESULTS

TOP ABSTRACT INTRODUCTION MATERIALS AND METHODS RESULTS DISCUSSION REFERENCES

Identification of GABAergic interneurons in the thalamus

We studied muscarinic effects on interneurons and interneuron-mediated inhibition in thalamocortical cells in the lateral posterior nucleus (LPN) and LGN of the rat's thalamus using the whole-cell recording technique (Fig. 1). Once the whole-cell configuration was formed, thalamic interneurons could be unambiguously distinguished from thalamocortical cells by their distinct physiological properties, such as high input resistance and long membrane time constant (Fig. 1B,D) (Pape et al., 1994; Williams et al., 1996; Zhu and Uhlrich, 1997; Zhu et al., 1999a). Both interneurons and thalamocortical cells could generate bursts of action potentials. However, the intraburst firing frequency in interneurons (<150 Hz) was lower than that in thalamocortical cells (>250 Hz) (cf. Deschênes et al., 1984; Williams et al., 1996; Zhu and Lo, 1998, 1999; Zhu et al., 1999d). The morphology of 34 interneurons and 30 thalamocortical cells was recovered (Fig. 1A,C). Interneurons in LPN and LGN had the distinguishing dendritic and axonal branching pattern as described previously (Williams et al., 1996; Zhu and Uhlrich, 1997; Zhu and Lo, 1999), characteristic for GABAergic interneurons in the thalamus (Ohara et al., 1983; Ottersen and Storm-Mathisen, 1984; Webster and Rowe, 1984; Gabbott et al., 1986). Interneurons recorded from LPN and LGN showed no difference in input resistance and time constant (Fig. 1E). However, these two properties were sufficient to distinguish interneurons from thalamocortical cells; the distributions of input resistance and time constant from interneurons did not overlap with those from thalamocortical cells (Fig. 1E).

View larger version (27K): [in this window] [in a new window]   Figure 1.   Thalamic interneurons. A, C, Morphology of reconstructed thalamic interneurons in the lateral posterior nucleus (LP) and the dorsal lateral geniculate nucleus (dLGN). Note that these cells were obtained from different slices. B, D, Responses of these interneurons to depolarizing and hyperpolarizing current pulses. E, Plot of the time constants of morphologically identified interneurons (filled circles and filled squares for interneurons in LGN and LPN, respectively) and thalamocortical cells (open circles and open squares for thalamocortical cells in LGN and LPN, respectively) against their input resistances. No difference was found in the time constant () or input resistance (Ri) among interneurons (LP: 100.6 ± 7.2 msec, n = 7; LGN: 92.0 ± 3.8 msec, n = 27; t test, p = 0.31 for ; LPN: 518 ± 37 M, n = 7; LGN: 539 ± 29 M, n = 27; t test, p = 0.72 for Ri) or among thalamocortical cells (LPN: 16.6 ± 1.3 msec, n = 12; LGN: 17.9 ± 1.2 msec, n = 18; t test, p = 0.45 for ; LPN: 98 ± 9 M, n = 12; LGN: 102 ± 8 M, n = 18; t test, p = 0.77 for Ri) in LPN and LGN.

Effects of muscarinic activity on firing mode in thalamic interneurons

We have shown previously that adult thalamic interneurons can generate burst firing and oscillation (Zhu et al., 1999a). The same burst firing and oscillation were observed in this study (Fig. 2) (n = 51). A previous in vivo study has shown that thalamus-projecting, cholinergic cells in the brainstem fire tonic action potentials, and their firing rate increases during wakefulness (Steriade et al., 1990). To test whether a sustained increase of muscarinic receptor activity could modify the burst firing pattern of interneurons, we continuously applied 1 mM Mch, a muscarinic agonist (McCormick 1992a; Zhu and Uhlrich, 1998), through the bath solution. The application of Mch reversibly suppressed burst firing and bursting oscillation in thalamic interneurons (Fig. 2B,C). Similar effects were found by bath application of ACh (Fig. 2D), although ACh also induced a brief, quickly desensitizing depolarization resulting from the activation of nicotinic receptors (Zhu and Uhlrich, 1997).

View larger version (28K): [in this window] [in a new window]   Figure 2.   Responses of rat thalamic interneurons to bath application of Mch. A, Responses of a thalamic interneuron to depolarizing and hyperpolarizing current pulses. B-D, Bath application of 1 mM Mch (n = 22) or 0.5 mM ACh (n = 5) decreased the input resistance of the cell and switched its burst firing pattern to a tonic firing one. Note the reduction of action potential number in the initial burst after application of Mch (Control: 5.2 ± 0.6; Mch: 1.8 ± 0.3; n = 9; p < 0.0001) and large current needed to evoked action potentials during application of agonists. The responses were reversible (not shown for ACh application). E, Input resistance (Ctrl: 602 ± 32 M; Mch: 328 ± 31 M; n = 12; p < 0.0001) and resting membrane potential (Ctrl: 66.1 ± 1.3 mV; Mch: 67.7 ± 1.3 mV; n = 12; p = 0.13) of thalamic interneurons in control or with Mch in bath solution. Large filled circles represent average values (same in the following figures). F, Dose-dependence interneuron responses to bath solution of Mch (left, n = 4) and ACh (right, n = 3). Note the monotonically increasing effect on the input resistance but not on the resting potential. Sigmoid curves in F are the best fitting curves for the average data points from Mch (pKi = 4.50, nH = 0.48) and ACh (pKi = 4.02, nH = 0.45) responses.

Bath application of Mch also had a prominent effect on input resistance of interneurons, but not on resting membrane potential measured when muscarinic activity reached to steady state (Fig. 2E). Using the change of input resistance as an indicator, we measured the dose-response curve of both Mch and ACh (Fig. 2F). Mch and ACh began to show evident effect at ~10 µM concentration, and 1 mM Mch and 0.5 mM ACh activated most of the muscarinic receptors (~95%) in interneurons. No change in resting membrane potential was observed at different concentrations of the agonists.

Muscarinic receptor-evoked responses in thalamic interneurons

A brief application of Mch to the recording chamber was used to dissect the Mch-evoked responses in interneurons (Fig. 3A) (n = 21). Interneurons responded to the brief Mch application with a hyperpolarization, which had a latency of ~1-3 sec and a duration of ~16-28 sec. The hyperpolarization was followed by a depolarization, which had a latency of ~16-28 sec and a duration of ~20-35 sec. The amplitudes of the hyperpolarization correlated with those of the depolarization (Fig. 3B). This result suggests that if Mch induces a large hyperpolarization in an interneuron, it will also induce a large depolarization in the same cell. The muscarinic receptors exhibited little desensitization because successive applications of Mch showed little change in the amplitude of hyperpolarization or depolarization (Fig. 3C,D). We also tested the effect of bath application of 1 mM Mch on some of these cells. Bath application of Mch induced the expected decrease in input resistance (Ctrl: 611 ± 51 M; Mch: 332 ± 40 M; n = 5; p < 0.005) with little change in membrane potential (Ctrl: 64.0 ± 1.9 mV; Mch: 64.8 ± 1.8 mV; n = 5; p = 0.65).

View larger version (41K): [in this window] [in a new window]   Figure 3.   Involvement of muscarinic subtype 2 receptors in Mch-evoked responses in thalamic interneurons. A, Brief bath application of 1 mM Mch (4-8 sec) induced a hyperpolarization followed by a depolarization in current-clamp mode or an outward current followed by an inward current in voltage-clamp mode. Note that both hyperpolarization and depolarization were accompanied by a decrease in input resistance (Ctrl: 542 ± 35 M; Hyperpolarization: 397 ± 27 M; p < 0.0001; n = 21; Depolarization: 435 ± 27 M; n = 21; p < 0.0001). B, Plot of peak amplitudes of depolarization (4.2 ± 0.4 mV) against that of hyperpolarization (6.4 ± 0.6 mV) induced by brief application of Mch (r = 0.51; n = 21; ANOVA, p < 0.05). Linear line is a regression line. C, The muscarinic responses exhibited little desensitization to successive brief applications of 1 mM Mch but were blocked by bath application of 200 nM gallamine. D, Hyperpolarization (Ctrl: 5.6 ± 0.8 mV; Mch: 5.4 ± 0.7 mV; n = 5; p = 0.58) and depolarization (Ctrl: 3.9 ± 0.7 mV; Mch: 3.9 ± 0.4 mV; n = 5; p = 0.87) evoked by the first and second applications of Mch. E, Hyperpolarization (Ctrl: 6.5 ± 0.9 mV; Mch: 0.3 ± 0.2 mV; n = 6; p < 0.001) and depolarization (Ctrl: 4.1 ± 0.9 mV; Mch: 0.2 ± 0.1 mV; n = 6; p < 0.01) in control and with gallamine in bath solution.

Recent studies have shown that muscarinic subtype 2 (M2) receptors are highly expressed in the dendrite and soma of thalamic interneurons (Carden and Bickford, 1999; Plummer et al., 1999). We tested whether Mch-evoked responses in interneurons were mediated by M2 receptors. Bath application of 200 nM gallamine, a selective M2 receptor antagonist (Michel et al., 1990), reversibly blocked the muscarinic hyperpolarization and depolarization (Fig. 3C,E), indicating that the Mch-evoked responses were mediated mainly by M2 receptors.

Cellular mechanisms underlying the muscarinic responses in thalamic interneurons

An early study has shown that activation of M2 muscarinic receptors induces a delayed Ca²⁺ release from intracellular stores (Lechleiter et al., 1991). To test whether the muscarinic responses were mediated by increased concentration of intracellular Ca²⁺, we included 5 mM EGTA in the intracellular solution. We found that the depolarizing response to a brief application of Mch was blocked after loading EGTA for ~30 min, whereas the hyperpolarizing response changed little (Fig. 4A,B). Consistent with the EGTA blockade of intracellular Ca²⁺ increase, we found that continuous perfusion of 1 mM Mch with the bath solution resulted in a sustained hyperpolarization in membrane potential in cells loaded with EGTA (n = 2) (data not shown). No change in hyperpolarization and depolarization to a brief application of Mch was found in cells recorded with normal intracellular solution (Fig. 3C,D). These results suggest that the depolarizing response is mediated by muscarinic receptor-stimulated increase of intracellular Ca²⁺ in interneurons.

View larger version (18K): [in this window] [in a new window]   Figure 4.   Muscarinic activity enhances a potassium conductance in thalamic interneurons. A, Brief bath application of 1 mM Mch induced a hyperpolarization followed by a depolarization right after the formation of the whole-cell configuration. B, Depolarization was blocked after intracellular loading of 5 mM EGTA for 20-50 min (n = 4). C, I-V relationships obtained before (Ctrl) and after application of Mch. Note that current versus voltage (I-V) plots were obtained by ramping membrane potential from 130 or 125 to 55 or 40 mV over a period of 5-10 sec. D, Difference between control I-V relation and that obtained 5 sec after application of Mch reveals that Mch affected a relatively linear current that reversed at 100.5 ± 1.2 mV (n = 6).

Taking advantage of the isolated hyperpolarization, we studied its cellular mechanism using the single-electrode voltage-clamp technique. To do that, we obtained current versus voltage plots by ramping the voltage from 130 mV to 55 mV and measuring the current required to achieve the voltage. Current versus voltage plots of control and 5 sec after application of Mch were obtained in this way (Fig. 4C). The difference of current versus voltage plots revealed that Mch induced an outward current that reversed at approximately 100 mV, the expected potassium reversal potential (n = 3) (Fig. 4D). The outward current had a relatively linear I-V relationship. The same result was obtained from three other cells without loading EGTA. These results indicate that an enhancement of a potassium conductance underlies the Mch-evoked early hyperpolarization.

Because a hyperpolarization-activated cation current (I_h) is present in thalamic interneurons (Zhu et al., 1999b) and this current is enhanced when the intracellular Ca²⁺ concentration is raised (Hagiwara and Irisawa, 1989; Zhu and Uhlrich, 1998; Luthi and McCormick, 1999), we tested whether the Mch-evoked depolarization was mediated by I_h. The effect of Mch on I_h was examined by comparing the currents induced by stepping the command voltage from 60 to 100 mV at control and 20 sec after a brief application of Mch (Fig. 5A,C). This target voltage was chosen for two reasons. First, it elicits a near-maximal activation I_h. Second, it is near the reversal potential of the potassium current, thereby minimizing contamination from the muscarinic effects on the potassium current. In all seven cells tested, the slowly activated inward current, I_h, was increased after application of Mch, suggesting that I_h was upregulated by Mch. Bath application of 2 mM Cs⁺ (Fig. 5B), which blocks I_h, or intracellular loading of 5 mM EGTA (Fig. 5D), which suppresses the Ca²⁺-dependent modulation on I_h, abolished the effect, confirming that muscarinic activity upregulated I_h in interneurons.

View larger version (24K): [in this window] [in a new window]   Figure 5.   Muscarinic activity enhances two cation conductances in thalamic interneurons. A, C, Stepping voltage to 100 mV before (Ctrl) and 20 sec after application of 1 mM Mch in two interneurons revealed that the slowly activated inward current was augmented during the muscarinic response. The effect was blocked by bath application of 2 mM Cs+ (B; n = 7) or intracellular loading of 5 mM EGTA (D; n = 3). Note that the muscarinic activity generated a net outward current when cells were held at a depolarized membrane potential (0 mV), where Ih was largely inactivated (Zhu et al., 1999b). Calibration in D applies also to A-C. E, I-V relationships obtained from another interneuron before (Ctrl) and 35-45 sec after application of Mch, whereas 2 mM Cs+ was included in the bath solution. Inset, Subtracting the control I-V relation from that obtained after application of Mch reveals that Mch induced a linear inward current that reversed at 4.5 ± 3.9 mV (n = 4). F, Substituting extracellular Na+ ions with NMDG+ ions blocked the effect (n = 3).

Bath application of Cs⁺ also revealed a small Mch-evoked, sustained inward current when interneurons were held at 100 mV (Fig. 5B). We suspected that this current was mediated by a Ca²⁺-activated cation conductance (I_CAN), which also exists in thalamic interneurons (Zhu et al., 1999d). Given the small amplitude of the inward current, we chose only cells with large muscarinic depolarization to test whether Mch activated I_CAN. Current versus voltage plots, with voltage ramped from 100 mV to 40 mV, were obtained at control condition and ~40 sec after application of Mch to minimize contamination from the muscarinic effect on the hyperpolarizing conductance (Figs. 4B, 6A). With 2 mM Cs⁺ also included in the bath solution, the difference of the current plots revealed a relatively linear current activated by Mch (Fig. 5E and inset). The average reversal potential suggests that a cation conductance, presumably I_CAN, is activated by Mch. Substituting extracellular Na⁺ ions with NMDG⁺ ions, which blocked I_CAN, eliminated the effect (Fig. 5F), supporting the notion that the sustained inward current was mediated by I_CAN. Together, these results suggest that Mch-evoked depolarization in thalamic interneurons is mediated by the enhancement of both I_h and I_CAN.

To further confirm that the enhancement of I_h and I_CAN mediates the muscarinic depolarization, we tested the effect of Cs⁺ and NMDG⁺ ions on the depolarization evoked by brief application of Mch (Fig. 6A). Bath application of Cs⁺ ions and substitution of extracellular Na⁺ ions with NMDG⁺ ions increased the input resistance of interneurons, which results in increases in the hyperpolarizing response evoked by brief application of Mch (Fig. 6B). In contrast, the depolarizing response was partially blocked by 2 mM Cs⁺ and completely blocked after substitution of extracellular Na⁺ ions by NMDG⁺ ions (Fig. 6B). This is consistent with the hypothesis that I_h and I_CAN mediate the muscarinic depolarization.

View larger version (25K): [in this window] [in a new window]   Figure 6.   Two cation conductances mediate muscarinic depolarization in thalamic interneurons. A, Muscarinic depolarization induced by brief application of 1 mM Mch was partially blocked by bath application of 2 mM Cs+ and completely blocked by further substituting extracellular Na+ ions with NMDG+ ions. Note that these channel blockers increased the input resistance of the cell. B, Hyperpolarization (Ctrl: 5.8 ± 0.4 mV; Cs+: 7.0 ± 0.3 mV; p < 0.05; n = 6; Cs++NMDG+: 9.0 ± 0.4 mV; n = 6; p < 0.01) and depolarization (Ctrl: 3.7 ± 0.6 mV; Cs+: 1.8 ± 0.3 mV; p < 0.05; n = 6; Cs++NMDG+: 0.4 ± 0.2 mV; n = 6; p < 0.01) in control and with one or two channel blockers.

Together these results indicate that muscarinic activity activates both depolarizing and hyperpolarizing conductances in interneurons, with little desensitization. This explains why the prolonged bath application of Mch results in a large decrease in input resistance but little net change in membrane potential after muscarinic activity reaches the steady state.

Effects of muscarinic activity on interneuron-mediated inhibition in thalamocortical cells

We wished to examine whether the muscarinic effects on interneurons can modulate the interneuron-mediated inhibition in thalamocortical cells. We added NBQX and DL-AP5 in the bath solution to block the excitatory synaptic transmission and then stimulated interneurons directly by placing the stimulating electrodes within LPN or LGN. Direct stimulation of interneurons in LPN (n = 1) and LGN (n = 4) evoked a large depolarization, which could lead to a burst of two to six action potentials (Fig. 7A,B). Bath application of Mch suppressed the depolarization and transformed the bursts into a subthreshold response or single action potential response (Fig. 7B,C).

View larger version (24K): [in this window] [in a new window]   Figure 7.   Blockade of GABAB inhibition in thalamocortical cells by muscarinic activity. A, Schematic drawing of recording and stimulating electrodes indicates the location of the stimulating electrode, placed within the dendritic tree of the recorded interneuron (~200 µm away from the soma). B, Direct electric shock evoked a large depolarization and a burst of action potentials in an interneuron in the presence of 5 µM NBQX and 100 µM DL-AP5. Bath application of 1 mM Mch transformed bursting firing into single action potential responses. C, Number of action potentials, averaged over 8-16 trials, in control or with Mch in bath solution (Ctrl: 2.8 ± 0.4; Mch: 0.8 ± 0.1; n = 5; p < 0.01). Circles and squares represent data points obtained from acute and culture slices, respectively (same in F and Fig. 8). D, Schematic drawing of recording and stimulating electrodes indicates the location of the stimulating electrode, placed ~200-500 µm away from the soma of the recorded thalamocortical cell. E, Direct electric shock evoked a prolonged IPSP in a thalamocortical cell in the presence of 5 µM NBQX and 100 µM DL-AP5. Bath application of 10 µM PTX blocked the early component of the IPSP. The later component of the IPSP was blocked by 1 mM saclofen (n = 3) or 1 mM Mch. F, Amplitude of later IPSPs in control or with Mch in bath solution (Ctrl: 0.51 ± 0.09 mV; Mch: 0.03 ± 0.01 mV; n = 15; p < 0.0001).

We then tested whether suppression of burst responses in interneurons by Mch can subsequently affect the inhibition in thalamocortical cells. Because of the long dendrites of interneurons in LPN and LGN, we could locally excite a population of interneurons without activating their postsynaptic thalamocortical cells directly (Fig. 7D,E). Recording from postsynaptic thalamocortical cells showed that such stimulation evoked a prolonged IPSP (Fig. 7E). The IPSP appeared to have two components. The early part of the response was blocked by bath application of picrotoxin (PTX), a GABA_A receptor blocker, leaving a small and slow IPSP. This slow IPSP was blocked by bath application of saclofen, a GABA_B receptor blocker, or Mch (Fig. 7E,F), although Mch also induced a depolarization and an increase in input resistance in thalamocortical cells [data not shown; but see Zhu and Uhlrich (1998)]. The same muscarinic effect on GABA_B response was found in thalamocortical cells recorded from culture slices (Fig. 7F). Because the severed axons of reticular cells degenerate after 24 hr (Ohara et al., 1980), the results suggest that the interneuron-mediated GABA_B IPSP is largely blocked by muscarinic activity.

The muscarinic effect on interneuron-mediated GABA_A IPSP was also studied. In this experiment, we directly stimulated two populations of interneurons (proximally located vs distally located) by placing two stimulating electrodes at ~250 and ~500 µm away from the recorded thalamocortical cells, respectively (Fig. 8A). Both distal and proximal stimulation could evoke a prolonged IPSP in thalamocortical cells, and the GABA_A-mediated response could be isolated by the bath application of saclofen (Fig. 8B). Although bath application of Mch had a significant suppression on the distal stimulation-evoked GABA_A IPSP, it had much less effect on the proximal stimulation-evoked GABA_A IPSP (Fig. 8B-E). In several cases, the latter IPSP was actually enhanced (Fig. 8C), presumably reflecting a muscarinic increase of input resistance and depolarization in postsynaptic thalamocortical cells (Zhu and Uhlrich, 1998). A similar result was obtained from the culture slices (Fig. 8C-E). Although Mch produced a slightly larger suppression on the distal inhibition in culture slices than in acute slices, the difference was not statistically significant (Fig. 8E), suggesting that the inhibition induced by the direct stimulation was mediated primarily by the activation of interneurons. The results indicate that muscarinic activity selectively suppresses the distal, interneuron-mediated inhibition but on average has little effect on the local, interneuron-mediated inhibition.

View larger version (29K): [in this window] [in a new window]   Figure 8.   Selective suppression of distal interneuron-mediated GABAA inhibition in thalamocortical cells by muscarinic activity. A, Schematic drawing of recording and stimulating electrodes indicates the locations of two stimulating electrodes, placed ~250 and 500 µm away from the soma of the recorded thalamocortical cell, respectively. B, Direct electric shock at the proximal and distal locations evoked fast IPSPs in a thalamocortical cell in the presence of 5 µM NBQX, 100 µM DL-AP5, and 1 mM saclofen. The proximal shock-evoked IPSP was less sensitive to the bath application of 1 mM Mch, compared with the distal shock-evoked one. C, Amplitude of proximal shock-evoked IPSPs in control or with Mch in bath solution (Ctrl: 2.1 ± 0.40 mV; Mch: 1.9 ± 0.4 mV; n = 12; p = 0.52). D, Amplitude of distal shock-evoked IPSPs in control or with Mch in bath solution (Ctrl: 1.3 ± 0.4 mV; Mch: 0.6 ± 0.2 mV; n = 12; p < 0.001). E, Relative amplitude of the proximal shock-evoked and distal shock-evoked IPSPs with Mch in bath solution (Proximal: 91.0 ± 10.0%; Distal: 40.3 ± 8.7%; n = 12; p < 0.005). The values were normalized to control responses. Note that the muscarinic suppression on distal responses was slightly larger in culture slices than in acute slices (culture: 34.6 ± 5.5%; n = 6; acute: 59.6 ± 22.5%; n = 6; t test; p = 0.29). The values were normalized to proximal responses. F, Schematic drawing of recording and stimulating electrodes indicates the simultaneous recordings from two thalamocortical cells, located at ~250 and 500 µm away from the stimulating electrode. G, Direct electric shock-evoked fast IPSPs in a pair of simultaneously recorded thalamocortical cells in the presence of 5 µM NBQX, 100 µM DL-AP5, and 1 mM saclofen. The IPSP in the proximally located thalamocortical cell was less sensitive to the bath application of 1 mM Mch, compared with the distally located one. H, Relative amplitude of the IPSPs in proximally and distally located thalamocortical cells with Mch in bath solution (Proximal: 89.3 ± 10.3%; Distal: 43.9 ± 8.1%; n = 10; p < 0.005). The values were normalized to control responses. Note that the muscarinic suppression on distal responses was slightly larger in culture slices than in acute slices (culture: 44.5 ± 6.0%; n = 5; acute: 60.1 ± 9.9%; n = 5; t test; p = 0.37). The values were normalized to proximal responses.

To confirm that Mch selectively suppressed the distal inhibition, we performed another experiment with a slightly modified stimulating and recording setting. In this experiment, only one population of interneurons was stimulated directly. Two thalamocortical cells, located at ~250 and 500 µm away from the stimulating electrode, were then recorded simultaneously (Fig. 8F). Bath application of Mch selectively suppressed the IPSP in the distally located thalamocortical cell, but on average had little effect on that in the proximally located one (Fig. 8G,H). In fact, the IPSP in the proximally located thalamocortical cells was often boosted by Mch application (Fig. 8G,H). Again, Mch produced a slightly larger (but not significant) suppression on the distal inhibition in culture slices than in acute slices (Fig. 8H). These results confirm that muscarinic receptor activity selectively suppresses the long-range inhibition.

	DISCUSSION

TOP ABSTRACT INTRODUCTION MATERIALS AND METHODS RESULTS DISCUSSION REFERENCES

In this study, we demonstrated that activation of muscarinic receptors in thalamic interneurons switched their firing mode from bursting to tonic. This effect is mediated by the muscarinic subtype 2 receptors and involves enhancement of at least three conductances: a linear potassium conductance, I_h, and I_CAN. Upregulation of these conductances has little net effect on the resting membrane potential in interneurons but causes a large decrease in their input resistance. The muscarinic activity also effectively modulates the interneuron-mediated inhibition in thalamocortical cells by blocking GABA_B inhibition, suppressing distal GABA_A inhibition, and maintaining local GABA_A inhibition relatively unchanged (Fig. 9).

View larger version (30K): [in this window] [in a new window]   Figure 9.   Schematic drawing shows the patterns of interneuron-mediated inhibition in thalamocortical cells with or without muscarinic activity. Note that the sustained muscarinic activity suppresses long-range, slow dendrite- and axon-originated inhibition, but not local, fast dendrite-originated inhibition in thalamocortical cells caused in part by the muscarinic uncoupling of the distal dendrites of thalamic interneurons.

Muscarinic effect on burst firing in interneurons

Recently, we have shown that the change of membrane potential can modulate burst firing in interneurons (Zhu et al., 1999a). Here we report a more profound muscarinic effect on input resistance, which can effectively switch the firing mode in these cells. Because thalamus-projecting, cholinergic cells in the brainstem fire tonic action potentials and their firing rate increases during wakefulness (Steriade et al., 1990), we focused on the effect of tonic muscarinic receptor activation, presumably more physiologically relevant, on the membrane properties and firing mode of interneurons in this study. We found that a sustained activation of muscarinic receptors induces a decrease in input resistance and a transformation of firing pattern from bursting to tonic firing. The switch of firing pattern likely results from the decrease in input resistance, which is crucial for interneurons to fire bursts of action potentials (Zhu et al., 1999a). This effect may be mediated by two mechanisms. First, reducing input resistance in interneurons may directly prevent the active conductances from generating large depolarizations necessary for bursting (our unpublished simulation data). Second, reducing input resistance may also indirectly suppress bursting by electrotonically uncoupling the axonal action potential initiation zone from the distal dendrites that appear to host a large amount of active conductances crucial for burst generation in interneurons (Zhu et al., 1999d).

Muscarinic receptor-mediated responses in thalamic interneurons

An early in vivo study has shown that activation of brainstem cholinergic cells, which project to the thalamic nuclei, can induce a large hyperpolarization in geniculate interneurons (Ahlsén et al., 1984), caused presumably by activation of a potassium conductance (McCormick and Pape, 1988). Here, we show that in addition to a potassium conductance, activation of muscarinic receptors also enhances I_h and I_CAN. The discrepancy can be explained by the different recording techniques used. The sharp electrodes used in the previous two studies can cause Ca²⁺ influx into the cells (Staley et al., 1992), which may subsequently occlude the Mch effects on I_h and I_CAN. Consistent with this view, we found that buffering intracellular Ca²⁺ ions with EGTA selectively blocked the depolarizing currents.

Functional considerations

Inhibitory neurons can provide many forms of inhibition that are critical for various information processes (Connors et al., 1988; Ferster and Jagadeesh, 1992; Gibson et al., 1999; Huntsman et al., 1999; Anderson et al., 2000; Zhu and Lo, 2000). A tight regulation of the inhibition patterns thus becomes crucially important for the brain to properly perform different tasks (Reyes et al., 1998; Xiang et al., 1998; Finnerty et al., 1999; Larkum et al., 1999). In the thalamus, thalamocortical cells in the lateral posterior (Zhu and Lo, 1997) and lateral geniculate nuclei (Ahlsén et al., 1985; Crunelli et al., 1988; Bal et al., 1995) receive inhibitory inputs from reticular cells. This recurrent inhibition is controlled by neuromodulators, which determine the membrane potentials and firing pattern of reticular cells. However, local thalamic interneurons show little change in their resting membrane potential during the steady-state activation of muscarinic receptors. Instead there is a large decrease in input resistance, which effectively switches the firing mode in interneurons from bursting to tonic and changes their electrotonic properties.

A switch of firing pattern from bursting to tonic will suppress GABA_B inhibition in thalamocortical cells (Huguenard and Prince, 1994; Destexhe and Sejnowski, 1995; Kim et al., 1997). Indeed, the interneuron-mediated GABA_B response in thalamocortical cells is largely blocked by muscarinic activity, whereas on average the local GABA_A response changes little. This result is in the line with the previous in vivo finding that activation of brainstem cholinergic cells suppresses only slightly the GABA_A receptor-mediated inhibition in thalamocortical cells, whereas the GABA_B receptor-mediated inhibition is completely eliminated (Ahlsén et al., 1984; Hu et al., 1989; Curró Dossi et al., 1992).

The dendrites of thalamic interneurons release the neurotransmitter GABA (Ralston, 1971; Cox et al., 1998). Without the activation of muscarinic receptors, interneurons are likely to be electrotonically compact because of their high input resistance (cf. Zhu, 2000). When muscarinic receptors are activated, the input resistance is reduced by ~50%, and this may isolate their distal dendrites electrotonically from each other (Bloomfield and Sherman, 1989). The distal dendritic triads will function as independent units, being excited by the presynaptic retinal afferents and then directly inhibiting only the postsynaptic dendrites of thalamocortical cells. Thus, we expect that the long-range inhibition (e.g., activation of one distal dendritic terminal and release of GABA at another one) will be suppressed. Our results do show that although the local GABA_A response remains unchanged after muscarinic receptor activation, the distal GABA_A response is substantially suppressed. Consistent with this idea, an in vivo study has reported blockade of long-range inhibition in thalamocortical cells by iontophoretic application of acetylcholine in LGN (Eysel et al., 1986).

Because muscarinic activity reduces input resistance and increase electrotonic isolation, the dendrites will become less excitable, and the soma and axon will be more isolated from the dendrites in interneurons (Bloomfield and Sherman, 1989; Zhu, 2000). Thus, the axon will be less sensitive to distal inputs and less likely to reach firing threshold. As expected, muscarinic receptor activation does suppress the axonal firing induced by direct dendritic activation in interneurons, transforming the burst firing into single action potential or subthreshold responses. The notion is further supported by an elegant in vivo report (Curró Dossi et al., 1992), which illustrates that when brainstem cholinergic cells are activated, the GABA_B receptor-mediated inhibition is completely eliminated. In addition, the axon-mediated GABA_A inhibition is also substantially suppressed, whereas the dendrite-mediated GABA_A inhibition is not reduced. Instead, in many instances it is enhanced. Congruent with the observations, the IPSPs resulting from the activation of the distal dendrites are substantially reduced by the muscarinic activity, whereas those caused by the local dendrites are less reduced and sometimes may even be boosted by muscarinic activity.

In addition to muscarinic effects on the intrinsic membrane properties of interneurons, muscarinic activity at presynaptic release sites and postsynaptic thalamocortical cells may also play a role in sharpening the interneuron-mediated inhibition. In the thalamus, although muscarinic receptors are only weakly expressed in some axonal terminals that originate presumably from the cholinergic nuclei in the brainstem, they are highly expressed in the presynaptic dendritic terminals of interneurons (Carden and Bickford, 1999; Plummer et al., 1999). These muscarinic receptors may suppress neurotransmitter release (Gil et al., 1997; Cox and Sherman, 2000) by reducing dendritic excitability (see above discussions) or suppressing the vesicle release machinery, or both. This effect may account for the general suppression of interneuron-mediated GABA_A responses (also see Curró Dossi et al., 1992; Pape and McCormick 1995). However, the local, dendrite-originated GABA_A inhibition is not significantly changed by muscarinic activity. This presumably reflects weaker muscarinic suppression of the local, dendrite-originated GABA_A inhibition, as well as the muscarinic effects on input resistance and membrane potential at postsynaptic thalamocortical cells (McCormick 1992a; Zhu and Uhlrich, 1998), which should amplify the voltage response of GABA_A current.

Conclusions

Neuromodulators, including acetylcholine, have been shown to control the firing pattern of several types of neurons by regulating their membrane potential (McCormick, 1992b; Steriade et al., 1997). Here we have demonstrated that acetylcholine can switch the firing patterns in thalamic interneurons, but by a different cellular mechanism. Instead of altering membrane potential, sustained muscarinic activity decreases the input resistance in interneurons. This muscarinic regulation of input resistance provides a new cellular mechanism for in vivo cholinergic suppression of long-range, slow dendrite- and axon-originated inhibition, and boosts local, fast dendrite-originated inhibition in thalamocortical cells (summarized in Fig. 9). The muscarinic promotion of local, fast inhibition should increase both the spatial and temporal resolution of the sensory signals, which is desired for periods of alertness or arousal.

It is now clear that in addition to the axon, the dendrites of a large variety of neurons can release neurotansmitters in an activity-dependent manner (Hausser et al., 1995; Maletic-Savatic and Malinow, 1998; Schoppa and Westbrook, 1999; Zilberter et al., 1999; Chen et al., 2000). However, whether the neurotransmitter release at these two sites is coordinated and how it is coordinated are largely unknown. Here, we provide evidence that dendritic and axonal release sites are tightly regulated by muscarinic activity. Our results also show that dendritic compartments of thalamic interneurons can be dynamically coupled and uncoupled, which is presumably dependent on the behavioral states.

	FOOTNOTES

Received Sept. 21, 2000; revised Dec. 1, 2000; accepted Dec. 7, 2000.

This work was supported by National Institutes of Health (R.M.), the Max-Planck Society, the Alexander von Humboldt Foundation, the NARSAD Foundation, and the Norwegian Research Council. J.J.Z. is the Naples Investigator of the NARSAD Foundation. We thank Drs. Bert Sakmann and Roberto Malinow for their support, Drs. Josh Huang, Fu-Sun Lo, Marie-Claude Perreault, Ed Ruthazer, and Ken Seidenman for their helpful discussions and comments, and Dr. Yi Qin for excellent technical assistance.

Correspondence should be addressed to J. Julius Zhu, Cold Spring Harbor Laboratory, Jones 1 Bungtown Road, Cold Spring Harbor, NY 11724. E-mail: Zhuju{at}cshl.org.

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