QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

HOME  |   SEARCH  |   ARCHIVE  |   SUBSCRIBE  |   CONTACT  |   HELP

This Article Abstract Full Text (PDF) Submit an eLetter Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Web of Science (12) Citing Articles via Google Scholar Google Scholar Articles by Magoski, N. S. Articles by Kaczmarek, L. K. Search for Related Content PubMed PubMed Citation Articles by Magoski, N. S. Articles by Kaczmarek, L. K.

Next Article 

The Journal of Neuroscience, January 1, 2002, 22(1):1-9

Protein Kinase Modulation of a Neuronal Cation Channel Requires Protein-Protein Interactions Mediated by an Src homology 3 Domain

Neil S. Magoski¹, Gisela F. Wilson², and Leonard K. Kaczmarek¹

¹ Department of Pharmacology, Yale University, New Haven, Connecticut 06520, and ² Department of Biology, University of Michigan, Ann Arbor, Michigan 48109

	ABSTRACT

TOP ABSTRACT INTRODUCTION MATERIALS AND METHODS RESULTS DISCUSSION REFERENCES

Accumulating evidence suggests that many ion channels reside within a multiprotein complex that contains kinases and other signaling molecules. The role of the adaptor proteins that physically link these complexes together for the purposes of ion channel modulation, however, has been little explored. Here, we examine the protein-protein interactions required for regulation of an Aplysia bag cell neuron cation channel by a closely associated protein kinase C (PKC). In inside-out patches, the PKC-dependent enhancement of cation channel open probability could be prevented by the src homology 3 (SH3) domain, presumably by disrupting a link between the channel and the kinase. SH3 and PDZ domains from other proteins were ineffective. Modulation was also prevented by an SH3 motif peptide that preferentially binds the SH3 domain of src. Furthermore, whole-cell depolarizations elicited by cation channel activation were decreased by the src SH3 domain. These data suggest that the cation channel-PKC association may require SH3 domain-mediated interactions to bring about modulation, promote membrane depolarization, and initiate prolonged changes in bag cell neuron excitability. In general, protein-protein interactions between ion channels and protein kinases may be a prominent mechanism underlying neuromodulation.

Key words: Aplysia; bag cell neurons; cation channel; phosphorylation; protein kinase C; protein-protein interaction; SH3 domain; excitability.

	INTRODUCTION

TOP ABSTRACT INTRODUCTION MATERIALS AND METHODS RESULTS DISCUSSION REFERENCES

A fundamental mechanism underlying neuromodulation is the phosphorylation of ion channels by protein kinases (Levitan and Kaczmarek, 1997). In some cases, the ion channel and the kinase appear to be physically associated with one another (Holmes et al., 1996; Tsunoda et al., 1997; Yu et al., 1997; Tibbs et al., 1998; Davare et al., 2001), and this association can at times be robust enough to preserve kinase-dependent modulation either in cell-free patches (Bielefeldt and Jackson, 1994; Yu et al., 1997; Wilson et al., 1998) or after reconstitution into bilayers (Rehm et al., 1989; Chung et al., 1991). The colocalization and targeting of kinases to ion channels requires a means of orchestrating protein-protein interactions. The "signaling module" theory of protein-protein interaction proposes that association occurs through the binding of a larger section [(50-100 amino acids (aa)] of one protein, called a domain, to a smaller portion (5-10 aa) of a second protein, called a motif or binding peptide sequence (Cohen et al., 1995; Pawson, 1995; Pawson and Scott, 1997; Fanning and Anderson, 1999). A number of such domains have been implicated in ion channel function (for review, see Sheng and Wyszynski, 1997; Staub and Rotin, 1997). In the present work, we investigate the interactions between a cation channel, which is responsible for prolonged changes in Aplysia bag cell neuron excitability, and a closely associated kinase.

The bag cell neurons of Aplysia californica control egg-laying behavior through a profound change in excitability, called the afterdischarge (Kupfermann, 1967; Kupfermann and Kandel, 1970; Pinsker and Dudek, 1977; Rothman et al., 1983; Conn and Kaczmarek, 1989). Stimulation of inputs to the bag cell neurons produces an ~30 min period of depolarization and action potential firing, followed by an ~18 hr refractory period during which another afterdischarge cannot be elicited. A pharmacological stimulus for initiating afterdischarges is Conus textile venom (CtVm), which elicits a normal afterdischarge and refractory period (Wilson and Kaczmarek, 1993; Wilson et al., 1996; Magoski et al., 2000). CtVm activates a nonselective cation channel that provides a depolarizing drive for the afterdischarge and is key to controlling bag cell neuron excitability. The cation channel is regulated by several kinases and phosphatases, including a protein kinase C (PKC)-like enzyme that is colocalized with the cation channel in excised, inside-out patches (Wilson and Kaczmarek, 1993; Wilson et al., 1998). To determine whether the association between the kinase and the cation channel involves specific forms of protein-protein interactions, we have tested the ability of different interaction domains and motifs to interfere with the functional link between channel and kinase in excised, inside-out patches. We have also used whole-cell recording to introduce interaction domains into bag cell neurons and observe their effects on cation channel-induced depolarization. Our data indicate that the cation channel-kinase "association" requires src homology 3 (SH3) domain protein-protein interactions, and that disruption of this interaction prevents channel modulation and attenuates the long-lasting depolarization produced by cation channel activation.

	MATERIALS AND METHODS

TOP ABSTRACT INTRODUCTION MATERIALS AND METHODS RESULTS DISCUSSION REFERENCES

Animals and cell culture. Adult A. californica weighing 100-200 gm were obtained from Marine Specimens Unlimited (San Francisco, CA) or Marinus Inc. (Long Beach, CA). Animals were housed in an ~400 l aquarium containing continuously circulating, aerated Instant Ocean (Aquarium Systems, Mentor, OH) salt water at 14°C on an ~12 hr light/dark cycle and were fed Romaine lettuce three times a week. All experiments were performed at room temperature (18-20°C).

For primary cultures of isolated bag cell neurons, animals were anesthetized by an injection of isotonic MgCl₂ (~50% of body weight); the abdominal ganglion was removed and treated with neutral protease for 18 hr at 18-20°C (13.33 mg/ml; 165859; Boehringer Mannheim, Indianapolis, IN) and dissolved in normal artificial seawater (nASW); the nASW was composed of (in mM): 460 NaCl, 10.4 KCl, 11 CaCl₂, 55 MgCl₂, 15 HEPES, 1 mg/ml glucose, 100 U/ml penicillin, and 0.1 mg/ml streptomycin; pH adjusted to 7.8 with NaOH. The ganglion was then transferred to fresh nASW, and the bag cell neuron clusters were dissected from their surrounding connective tissue. Using a fire-polished Pasteur pipette and gentle trituration, neurons were dispersed in nASW onto 35 × 10 mm polystyrene tissue culture dishes (no. 25000; Corning, Corning, NY). Cultures were maintained in nASW for 1-3 d in a 14°C incubator.

Excised, inside-out patch-clamp recording. A single cation channel current was measured using an EPC-7 amplifier (List Electronics; Instrutech, Port Washington, NY) and the excised, inside-out patch-clamp method. Microelectrodes were pulled from 1.5 mm external diameter borosilicate glass capillaries (TW 150 F-4; World Precision Instruments, Sarasota, FL) and fire- polished to a resistance of 2-5 M when filled with nASW (composition as above but lacking glucose, penicillin, and streptomycin). After excision, the cytoplasmic face was bathed with artificial intracellular saline composed of (in mM): 500 K-aspartate, 70 KCl, 0.77 CaCl₂, 1.2 MgCl₂, 10 HEPES, 11 glucose, 0.77 EGTA, and 10 glutathione; pH adjusted to 7.3 with KOH; the calculated free [Ca²⁺] was ~1 µM. Regarding the calculated free [Ca²⁺], we used salts of the highest purity grade [from J. T. Baker Chemical Company (Phillipsburg, NJ), Mallinckrodt (Hazelwood, MD), or Sigma (St. Louis, MO)], and care was taken to measure amounts of CaCl₂ and EGTA accurately to ensure that the free [Ca²⁺] was as close as possible to the calculated theoretical value (calculation performed with the CaBuffer program, courtesy of Dr. L. Schlichter, University of Toronto, Toronto, Canada). Data were acquired with an IBM-compatible personal computer, a Digidata 1200 analog-to-digital converter (Axon Instruments, Foster City, CA), and the Fetchex acquisition program pClamp (version 6.02; Axon Instruments). Current was filtered at 1 kHz with a Bessel filter (Frequency Devices, Haverhill, MS) and sampled at 10 kHz. Data were gathered in 1-3 min intervals while holding the patch at 60 mV or, to avoid occasional contamination by Ca²⁺-activated K⁺ currents, at 80 mV.

Whole-cell current-clamp recording. Membrane potential responses to CtVm (see below) were measured with the EPC-7 amplifier and the tight-seal, whole-cell method. Microelectrodes (glass capillaries as above) were fire- polished to a resistance of 1-2 M when filled with artificial intracellular saline. [Composition was as described above, but CaCl₂ was reduced to 0.595 mM for a calculated free [Ca²⁺] of ~300 nM and supplemented with 5 mM ATP (grade 2, disodium salt; Sigma A3377) and 0.1 mM GTP (type 3, disodium salt; Sigma G8877)]. The ~300 nM concentration of free intracellular Ca²⁺ is based on the fura-imaging and ion-sensitive electrode measurements of cytosolic Ca²⁺ concentrations in cultured bag cell neurons that were obtained by our and other laboratories (Fisher et al., 1994; Knox et al., 1996; Magoski et al., 2000). The extracellular solution was nASW (composition as initially described). In some cases, 50 µM PKC_19-36 pseudosubstrate inhibitor peptide (Sigma P8462) or a 300 nM concentration of either src SH3 domain or yes SH3 domain was included in the intracellular saline. Data were acquired with the Clampex acquisition program of pClamp to either monitor membrane potential or inject current to measure input resistance. Voltage was taken from the EPC-7 V-reference and sampled at 1 Hz to monitor membrane potential or 2 kHz for input resistance assessment. Measurements were made from 50 mV, which was maintained as necessary by constant current injection from the EPC-7.

Reagents and drug application. Bacterially expressed, purified interaction domain fusion proteins and motif peptides were generous gifts from Dr. B. K. Kay (University of Wisconsin, Madison, WI). The original constructs were generated by amplifying predicted regions encoding the interaction domains by PCR, followed by subcloning into glutathione S-transferase (GST) gene fusion vectors as described by Gee et al. (1998), Pirozzi et al. (1997), and Sparks et al. (1996). Motif peptides were synthesized as described by Pirozzi et al. (1997).

CtVm lyophilate was generously provided by Dr. B. M. Olivera (University of Utah, Salt Lake City, UT). Adult specimens of the molluscivorous snail C. textile were collected from the ocean around the island of Marinduque in the Philippines. Venom ducts were dissected out of an animal and placed on an ice- cold metal spatula. The duct was then cut into 2 cm sections and the venom was extruded by squeezing with forceps. The venom was then lyophilized in a vacuum centrifuge and stored at 80°C for subsequent extraction. To prepare the crude CtVm, procedures were performed at 4°C, and the lyophilized venom was made up in 0.5% (v/v) trifluoroacetic acid (TFA) for a final protein concentration of 5% (w/v). The CtVm was vortexed for 2 min and sonicated for 2 min, in an alternating manner, for a total of 18 min. The mixture was then centrifuged at 15,000 × g for 12 min, and the supernatant was collected. A second aliquot of TFA was added to the pellet (final protein concentration of 10% w/v), and the protocol was repeated. The supernatants were pooled, divided into aliquots, and frozen at 80°C.

CtVm and other drugs were introduced into the bath by pipetting a small volume (<10 µl) of concentrated stock solution into the Petri dish (2 ml volume). Care was taken to pipette the stock near the side of the dish and as far away as possible from the patch or neuron. CtVm was diluted to a final protein concentration of ~100 µg/ml. ATP (grade 2, disodium salt; Sigma A3377) was diluted to a final concentration of 1 mM.

Analysis. To determine single- channel open probability (P_O) and make statistical descriptions of channel kinetics, event lists were made from single- channel data files by using the half-amplitude threshold criterion of the Fetchan analysis program of pClamp (Colquhoun and Sigworth, 1995). For analysis, most data did not require additional filtering below the 1 kHz used during acquisition; however, to avoid inclusion of noise-related "events" as channel openings, some data were filtered a second time by using the Fetchan digital Gaussian filter to a final cutoff frequency of 500 Hz. For display in figures, some data were filtered to a final cutoff frequency of 500 or 250 Hz. The Pstat analysis program of pClamp was used to read events lists and determine the P_O for a given data file, either automatically or manually, but in both cases the following formula was used: P_O = (t₁ + t₂ + ... t_n)/N × t_tot, where t equals the amount of time that n channels are open, N equals the number of channels in the patch, and t_tot equals the time interval over which P_O is measured. The number of channels in the patch was determined by counting the number of overlapping unitary current levels, particularly at more positive voltages (up to 20 mV). Pstat was also used to generate single- channel open and closed dwell-time histograms and fit them with a sum of exponentials describing the kinetic behavior of the channel. The time interval (x-axis) was binned logarithmically, and histograms were fit by using the maximum likelihood estimator method (Colquhoun and Sigworth, 1995) and a simplex search, which was given the number of exponential and estimated time constants () at the start. Maximum likelihood fitting allowed comparisons between fits with different numbers of exponentials, typically by using the log likelihood ratio (LLR) of one model with a given number of one exponential versus another model with a larger number of exponentials. Models were considered not different if the LLR was 2. Kinetic analysis was performed exclusively on patches that contained only one cation channel, as determined by a consistent display of only one open current level over several minutes, again at more positive voltages (up to 20 mV).

The magnitude of the CtVm-induced depolarization was taken as the difference between the pre-CtVm potential (maintained at 50 mV) and the peak of the depolarization. If the CtVm elicited action potentials, the peak depolarization was measured at the potential just after the cessation of spiking. As an assay of neuronal viability, input resistance was measured with a steady-state response to a 10-50 pA hyperpolarizing current injection and Ohm's law.

Data are presented as the mean and SEM. Statistical analysis was performed by using Instat (version 2.01; GraphPad Software Inc., San Diego, CA). Student's t test was used to test for differences between two means. Data were considered significantly different at p < 0.05.

	RESULTS

TOP ABSTRACT INTRODUCTION MATERIALS AND METHODS RESULTS DISCUSSION REFERENCES

ATP enhances cation channel activity

In the present study, cation channels were recorded using excised, inside-out patches from cultured Aplysia bag cell neurons. We have demonstrated previously that application of ATP to the cytoplasmic face of a patch containing a cation channel results in an increase in P_O. This is attributable to the phosphorylation of the cation channel, or some adjacent protein, by a closely associated PKC-like enzyme (Fig. 1A) (Wilson et al., 1998). This channel-kinase association or "complex" can be defined in functional terms; i.e., because the regulation of the channel by PKC persists in excised patches, the kinase must be associated with the channel in some manner, although we cannot say whether this association is direct or not. Cation channels were readily identified on the basis of a conductance of 25-30 pS (~2 pA inward current at 60 mV), an increased P_O with depolarization, a lack of voltage-dependent inactivation, and a distinct kinetic profile of a three exponential component description for closed times and a two exponential component description for open times (Wilson and Kaczmarek, 1993; Wilson et al., 1996). When the cytoplasmic face of the patch was exposed to 1 mM ATP, there was a rapid and sustained elevation of cation channel P_O lasting for the remainder of the recording period (up to 20 min) (Fig. 1B). In experiments on 21 patches, performed as parallel controls throughout the present study, ATP produced an increase in cation channel P_O from a mean control value of ~0.1 to a mean final level of ~0.25 (Fig. 1C). Enhancement of activity occurred regardless of initial P_O, although larger proportional increases were more often detected from lower starting P_O levels. For patches with channels showing an initial P_O of <0.1, the fold increase in P_O with ATP was 19.9 ± 10.1 (n = 13), whereas for patches with channels whose initial P_O was >0.1, the fold increase was 1.7 ± 0.2 (n = 8) (see also Wilson et al., 1998).

View larger version (15K): [in this window] [in a new window]   Figure 1.   ATP increases cation channel PO in excised, inside-out patches from cultured bag cell neurons. A, Model of the cation channel based on Wilson et al. (1998). Under physiological conditions, the channel passes Na+, K+, and Ca2+. ATP is used as a phosphate source in a phosphotransfer reaction catalyzed by a closely associated PKC-like enzyme (PKC). This phosphorylation of either the cation channel itself or a nearby protein results in enhanced activity. The purpose of the present study was to examine the nature of the interaction between the cation channel and the kinase. B, Top trace, At a holding potential of 60 mV, cation channel activity was observed as unitary inward current steps of ~2 pA. For this and subsequent figures, the closed state (C) is at the top, and the open state (O) is at the bottom of the trace. Bottom trace, Bath application of 1 mM ATP to the cytoplasmic face of the patch resulted in a marked and sustained increase in PO from 0.012 to 0.093. The PO rose to its elevated level within 10 sec of adding ATP; most likely, diffusion of ATP from the site of bath application (pipetting location; see Materials and Methods) was the determining factor in this time course. C, Grouped data for control experiments used in the present study. The increase in cation channel PO produced by ATP was statistically significant. The PO was calculated over the entire time of recording before and then after ATP (typically 1-3 min in each condition; see Materials and Methods). The n value refers to the number of patches.

Cation channel modulation is disrupted by src SH3 domain

We sought to examine the nature of the association between the cation channel and the kinase by applying various protein-protein interaction domains and binding peptides to the cytoplasmic face as a means of disrupting the channel-kinase association and preventing the ATP response. We tested the potential role of SH3 domains in mediating the cation channel-kinase association by using GST fusion proteins of SH3 domains from several proteins. The most effective domain tested was the SH3 domain from src itself, which is part of one of the noncatalytic regions of the tyrosine kinase molecule (Koch et al., 1991). When the SH3 domain from src (amino acids 87-143; 60-100 nM) was applied to the cytoplasmic face of a cation channel-containing patch and then followed by ATP, the expected elevation of P_O failed to occur (n = 7) (Fig. 2A,C). On its own, the src SH3 domain did not alter cation channel activity (n = 5) (Fig. 2C). Interestingly, the ATP-induced increase in cation channel P_O was not affected by GST fusion proteins of SH3 domains from yes, a tyrosine kinase related to src (150-375 nM; n = 4) (Fig. 2B,C, left traces), or grb2, a prototypical scaffolding protein, (N-terminal SH3 domain; 100-300 nM; n = 4) (Fig. 2B,C, right traces); in addition, these domains did not alter channel activity on their own (data not shown). This suggests that the inhibition of the ATP response by the src SH3 domain is attributable to a specific interaction between this domain and the channel-kinase complex and is not the result of some nonspecific effect of the domain storage buffer or the GST tag.

View larger version (43K): [in this window] [in a new window]   Figure 2.   The ATP-induced increase in channel PO is blocked by the src SH3 protein-protein interaction domain. A, Application of a 100 nM concentration of the GST fusion protein of the SH3 domain from the tyrosine kinase src prevented the enhancement of cation channel PO by ATP (0.005 in src SH3 vs 0.005 in src SH3 plus 1 mM ATP). The domain was bath-applied to the cytoplasmic face of the patch, which in this case contained two channels, and was followed by ATP ~2 min later. The patch was held at 60 mV. B, The block is specific to the src form of the SH3 domain, because GST-fused SH3 domains from the related tyrosine kinase yes (left traces) (PO = 0.014 in 375 nM yes SH3 vs 0.116 in yes SH3 plus 1 mM ATP) or the N-terminal SH3 domain from the grb2 adaptor protein (right traces; PO = 0.014 in 300 nM grb2N SH3 vs 0.116 in grb2N SH3 plus 1 mM ATP) fail to inhibit the ATP-induced PO increase. Both patches were held at 60 mV. C, Summary data for the effect of SH3 domains on the cation channel response to ATP. The src SH3 domain had no effect itself on channel PO; furthermore, it prevented ATP from enhancing PO. This is in contrast to the SH3 domains from yes or grb2N, neither of which impeded the significant elevation of PO by ATP. For C and E, the n values refer to the number of patches; n.s., Not significant. D, Exposure to the GST-fused PDZ interaction domain from the enzyme NOS did not prevent ATP from increasing cation channel PO (0.208 in 130 nM NOS PDZ vs 0.573 in NOS PDZ plus 1 mM ATP). The patch was held at 60 mV. E, Summary data for the effect of the NOS PDZ domain on cation channel activity and the ATP response. The PDZ domain did not alter cation channel activity, nor did it hinder ATP from significantly enhancing PO.

Another candidate interaction domain, and one that is found in scaffolding proteins known to organize complexes containing ionotropic glutamate receptors or certain K⁺ channels, is the PSD-95, disks-large, ZO-1 (PDZ) domain (Kennedy, 1995; Sheng and Wyszynski, 1997; Staub and Rotin, 1997). Application of the GST-tagged PDZ domain of nitric oxide synthase (NOS) (130-250 nM), however, had little impact on cation channel activity and did not prevent the ATP-induced increase in P_O (n = 4) (Fig. 2D,E). Similarly, a GST fusion protein of the PDZ domain from ₁- syntrophin (100 nM; n = 1) did not alter baseline cation channel behavior or affect the ATP response (data not shown).

Cation channel modulation is disrupted by src SH3 motif peptide

SH3 domains bind to a motif with the consensus amino acid sequence X₆PXXPX₆ (Pawson and Scott, 1997), and the specificity of interactions with distinct SH3 domains is determined primarily by the variable residues that flank the invariant prolines (boldface type) (Sparks et al., 1996). The prolines are thought to interact with the domain at a core binding groove, whereas the overall specificity of the interaction is mediated by binding between the flanking peptide amino acids and an adjacent specificity pocket on the domain (Nguyen et al., 1998). We tested the effects of exposing the cytoplasmic face of cation channel-containing patches to a 1 µM concentration of the src SH3 motif peptide (NH₂-LASRPLPLLPNSAPGQ-COOH; underlined residues are key for binding to the src form of the SH3 domain) (Sparks et al., 1996). The src SH3 motif peptide did not alter cation channel gating in the absence of ATP, and like the src SH3 domain, the peptide prevented the ATP-induced elevation of channel P_O (n = 4) (Fig. 3A,D). If the domain in the channel-kinase complex that interacts with the src SH3 motif peptide is an src-like SH3 domain, then a peptide that binds an SH3 domain different from that of src should not block the ATP response. To examine this, a second SH3 motif peptide, one that preferentially binds the abl SH3 domain (NH₂-SGSGSRPPRWSPPVPLPTSLDSR-COOH; underlined residues are key for binding to the abl form of SH3 domain) (Sparks et al., 1996), was applied at 1 µM to cation channel-containing patches. This second SH3 motif peptide did not alter cation channel behavior, nor did it disrupt the ability of ATP to increase channel P_O (n = 4) (Fig. 3B,D). As a secondary control for the vehicle or any contaminating solvents used during peptide synthesis, we also tested the effects of a WW motif peptide (NH₂-LKLPDYWESSAS-COOH; 1 µM). Like the abl SH3 motif peptide, the WW motif peptide had no effect on cation channel activity or the ATP-induced increase in P_O (n = 4) (Fig. 3C,D).

View larger version (21K): [in this window] [in a new window]   Figure 3.   An src SH3 motif peptide blocks the ATP-induced increase in PO. A, In keeping with an SH3-like domain-mediated interaction being involved in the channel-kinase association, a 1 µM concentration of an SH3 motif peptide (NH2-LASRPLPLLPNSAPGQ-COOH, src SH3 motif) that preferentially binds to the src SH3 domain blocked the ATP response (PO = 0.038 in controls vs 0.037 in src SH3 peptide vs 0.025 in src SH3 peptide plus 1 mM ATP). The src SH3 motif peptide was in the bath for ~2 min before the addition of ATP. The patch was held at 60 mV. B, To determine the specificity of the disruption of the channel-kinase complex by the src SH3 motif, a second SH3 motif (NH2-SGSGSRPPRWSPPVPLPTSLDSR-COOH, abl SH3 motif) was used that preferentially binds the abl SH3 domain over the src SH3 domain. A 1 µM dose of abl SH3 motif peptide failed to block the ATP response (PO = 0.023 in controls vs 0.037 in abl SH3 peptide vs 0.073 in abl SH3 peptide plus 1 mM ATP). C, As an additional control for nonspecific effects, a 1 µM concentration of the WW motif peptide (NH2-LKLPDYWESSAS-COOH) was applied to the cytoplasmic face of a cation channel-containing patch. This peptide did not interfere with the ATP-induced increase in PO (0.042 in controls vs 0.036 in WW peptide vs 0.055 in WW peptide plus 1 mM ATP). D, Summary data for the effect of src SH3, abl SH3, and WW motif peptides on the cation channel response to ATP. Of the two SH3 motif peptides, neither had a significant impact on channel activity; however, the src SH3 motif peptide but not the abl SH3 motif peptide inhibited the ATP-induced PO elevation. Furthermore, not only did the WW motif peptide have no effect on cation channel PO, it also failed to prevent an increase in PO by ATP. The n values refer to the number of patches; n.s., Not significant.

The investigation of cation channel modulation by the closely associated PKC also described a phosphatase-like activity present in excised patches that reverses the PKC effect after withdrawal of ATP, returning P_O to control levels (Wilson et al., 1998). Therefore, we tested whether the ATP response could be reversed by applying src SH3 motif peptide after the addition of ATP; i.e., can disruption of the SH3-dependent channel-kinase association allow the phosphatase to act, despite a maintained presence of ATP? Figure 4A shows an experiment in which the P_O of a very active, single cation channel (top trace) was markedly increased by the application of ATP to the cytoplasmic face of the patch (middle trace); subsequently, the addition of 1 µM src SH3 motif peptide produced a shift of the P_O back toward control levels (bottom trace). The same experiment was also performed on two other patches, containing multiple channels with lower starting P_O levels, and resulted in an identical outcome (P_O = 0.026 ± 0.012 in controls vs 0.105 ± 0.061 in ATP vs 0.022 ± 0.019 in ATP plus src SH3 motif peptide). These experiments also indicate that the phosphatase remains activated and associated with the membrane patch in the presence of the competing peptide.

View larger version (34K): [in this window] [in a new window]   Figure 4.   The src SH3 motif peptide reverses the ATP-induced increase in PO. A, A patch held at 60 mV displayed a single, rather active cation channel under control conditions (PO = 0.203). With the application of 1 mM ATP to the cytoplasmic face, the activity was markedly elevated (PO = 0.530). However, when 1 µM SH3 motif peptide was added along with the ATP, the activity of the channel fell back toward control levels (PO = 0.316). B, Given that this patch appeared to contain only a single cation channel, kinetic analysis was possible. The histograms represent single- channel closed and open dwell times, fit with a sum of exponentials by using the maximum likelihood estimator method and a simplex search (see Materials and Methods). The time constants of the exponentials are given in the inset of each graph, with the proportion or fractional contribution to the area under the curve of each exponential shown in brackets under its respective time constant. During the control period (top graphs), cation channel closed times were best described by three exponentials (C1, C2, and C3) and the open times were best described by two exponentials (O1 and O2). After exposure to ATP (middle graphs), the C1 or C2 showed little change; however, C3 was substantially reduced both in duration (a 65% decrease, from ~78 to ~27 msec) and in the proportion of events that it described (a 80% decrease, from 0.25 to 0.05). For open times during ATP, there was a modest shift, from O2 to O1, in the proportion of events described. After application of the SH3 motif peptide (bottom graphs), C3 values returned to near control levels. Similarly, the number of events described by O1 and O2 shifted back toward control levels.

Because the patch from the experiment depicted in Figure 4A contained only a single cation channel with a relatively high P_O (see Materials and Methods for information about determining the single- channel status of a patch), this allowed for kinetic analysis of the behavior of the channel and made it possible to separate the effect of dephosphorylation from any nonspecific effect of the src SH3 motif peptide. Under control conditions, cation channel closed times (Fig. 4B, top left graph) were best described by three exponentials (with time constants _C1, _C2, and _C3), whereas the open times (Fig. 4B, top right graph) were best fit with a function consisting of two exponentials (with time constants _O1 and _O2). ATP application resulted in an obvious decrease in both the duration and relative contribution of _C3 to the fitted function (Fig. 4B, middle left graph). At the same time, _C1 and _C2 underwent only slight changes, the exception being an increase in the number of events described by these time constants. With regard to open times in the presence of ATP, _O1 and _O2 showed only nominal changes in the number of events that they described (Fig. 4B, middle right graph). When the src SH3 motif peptide was applied after ATP, the duration and proportion of closures described by _C3 reverted back to control values (Fig. 4B, bottom left graph). In addition, there was a return toward control levels for the relative contribution of _O1 and _O2 (Fig. 4B, bottom right graph). This reversal of the kinetic profile of the channel, combined with the lack of an effect in the absence of ATP, suggests that the src SH3 motif-induced return of P_O to control levels, in the presence of ATP, is most likely attributable to disruption of the channel-kinase complex and subsequent phosphatase-dependent dephosphorylation, rather than an effect of the peptide on the channel itself.

We also applied a mixture of 100 nM src SH3 domain plus 1 µM src SH3 motif peptide to excised patches and tested the effect on the response to 1 mM ATP. This domain/peptide mixture also blocked the ability of ATP to increase cation channel P_O (0.062 ± 0.022 in domain/peptide vs 0.047 ± 0.012 in domain/peptide plus ATP; n = 3; not significant; data not shown).

Cation channel-mediated bag cell neuron depolarization is attenuated by PKC inhibition or src SH3 domain

To address the role of the SH3 domain-mediated interactions in the regulation of bag cell neuron excitability, we used interaction domains to disrupt the channel-kinase complex during whole-cell recording of cation channel-mediated depolarization in cultured bag cell neurons. Cation channels can be reliably activated in cultured bag cell neurons by the extracellular application of a crude extract of CtVm. Previous work suggests that CtVm activates cation channels and depolarizes bag cell neurons through the binding of a component(s), most likely peptidergic (Olivera et al., 1990), to a membrane receptor and initiation of both the production of second messengers and the activation of protein kinases (Wilson and Kaczmarek, 1993; Wilson et al., 1996, Magoski et al., 2000) (N. S. Magoski and L. K. Kaczmarek, unpublished observations). In many ways, the CtVm response of cultured bag cell neurons can be viewed as an in vitro analog of the afterdischarge itself.

In the present study, we first tested whether there was a contribution of the PKC pathway to the CtVm-induced depolarization of cultured bag cell neurons. Whole-cell current-clamp recordings were made from individual bag cell neurons, and the depolarizing CtVm responses of neurons dialyzed with control intracellular saline were compared with those dialyzed with a 50 µM concentration of the specific pseudosubstrate PKC inhibitor peptide PKC_19-36 (House and Kemp, 1987) (see Materials and Methods). As depicted in Figure 5, the depolarizing response is often multiphasic, perhaps because of the production of multiple second messengers after CtVm application (see above). Figure 5A (top traces) shows that previous dialysis with PKC_19-36 attenuated the CtVm-induced depolarization by approximately one-third with respect to controls (n = 4 and 4). We subsequently determined whether disruption of the channel-kinase complex with the src SH3 domain would affect the CtVm response. When bag cell neurons were dialyzed with a 300 nM concentration of src SH3 domain (n = 7 and 7) (Fig. 5A, middle traces), the depolarization evoked by CtVm was again attenuated. Moreover, this reduction in the CtVm-induced depolarization did not appear to be attributable to the buffer or to a nonspecific effect of the domain itself, because dialysis with 300 nM yes SH3 domain did not alter the peak depolarization compared with controls (n = 4 and 4) (Fig. 5A, bottom traces). Finally, bag cell neuron input resistance, a simple assay of excitability, was unaffected by dialysis, regardless of the contents of the intracellular saline (Fig. 5C). The fact that the depolarization was inhibited to an equal extent by both the PKC inhibitor and the src SH3 domain suggests that, other than PKC, modulatory components involved in eliciting the depolarization were unaffected by the src SH3 domain.

View larger version (18K): [in this window] [in a new window]   Figure 5.   CtVm-induced depolarization is reduced by both a PKC inhibitor and the src SH3 domain. A, Whole-cell current-clamp recordings of depolarizations elicited by CtVm (100 µg/ml) in cultured bag cell neurons. CtVm responses in neurons dialyzed with control intracellular saline (gray line) are displayed along with responses of neurons dialyzed with intracellular saline containing 50 µM PKC19-36, 300 nM src SH3 domain, or 300 nM yes SH3 domain (black line). Neurons were dialyzed for 45 min before CtVm bath application (arrow). The peak CtVm- induced depolarization for neurons dialyzed with either PKC19-36 (top traces) or src SH3 domain (middle traces) was reduced compared with controls; however, dialysis with yes SH3 domain (bottom traces) did not have a obvious impact on the peak depolarization. The control CtVm response depicted in the top traces shows some action potentials approximately halfway through the recording period; the spikes are truncated because of the low digitization rate and the display scale. Differences in the onset of the depolarization are not significant and are most likely attributable to experimental variability. B, Summary data for the effect of PKC19-36, src SH3 domain, or yes SH3 domain on the bag cell neuron membrane potential response to CtVm and on input resistance before CtVm application. Top graph, On average, both PKC19-36 and src SH3 domain dialysis significantly reduced the depolarization by approximately one-third compared with controls. The SH3 domain from yes did not produce any significant change in the CtVm-induced depolarization. Bottom graph, Input resistance (see Materials and Methods) was measured at the end of dialysis and just before the application of CtVm. Compared with controls, none of the agents introduced into the neurons had a significant effect on input resistance. The apparent but nonsignificant elevation after PKC19-36 introduction was attributable to a single neuron showing an inexplicable increase in input resistance over the dialysis period. The labels apply to both the top and bottom graphs, and the n values refer to the number of control and experimental neurons examined in a given set. n.s., Not significant.

	DISCUSSION

TOP ABSTRACT INTRODUCTION MATERIALS AND METHODS RESULTS DISCUSSION REFERENCES

Previous work has shown that much of the depolarizing drive and membrane potential instability of the afterdischarge originates from the activation of a defined cation conductance in the bag cell neurons (Kaczmarek and Strumwasser, 1984; Wilson and Kaczmarek, 1993; Wilson et al., 1996). The macroscopic as well as the underlying single-channel properties of this cation conductance are one of a slow, nonselective, Ca²⁺-permeable, Ca²⁺-sensitive, voltage-dependent, noninactivating current (Wilson et al., 1996; Magoski et al., 2000). Similar currents have been described in a number of molluscan and mammalian neurons, in which they contribute to bursting and repetitive firing (Wilson and Wachtel, 1974; Green and Gillette, 1983; Stafstrom et al., 1985; Swandulla and Lux, 1985; Alonso and Llinas, 1989). The afterdischarge is associated with changes in both the electrical and biochemical properties of the bag cell neurons (Conn and Kaczmarek, 1989). The activity of PKC, an enzyme that mediates several afterdischarge-associated biophysical changes (Strong et al., 1987; Knox et al., 1992), increases with onset of the afterdischarge (Wayne et al., 1999). With the same assay of excised, inside-out patches that was used in the present study, Wilson et al. (1998) showed that cation channel P_O is enhanced by the application of ATP, through the activity of a closely associated PKC-like enzyme. Thus, the increase in PKC activity is temporally consistent with the ability of the kinase to modulate cation channel behavior and suggests that this mechanism contributes to the electrical changes of the afterdischarge. The association of the cation channel with PKC is likely to promote cation channel modulation after a stimulus for initiating the afterdischarge.

We have now shown that application of either the SH3 domain from src or an src SH3 motif peptide (i.e., an optimized binding site for src SH3 domains) prevents the ATP-induced increase in cation channel P_O. This inhibition probably reflects a disruption of the protein complex containing the channel and the kinase. The effects of the src SH3 domain and peptide were specific, because SH3 or PDZ domains from other proteins, as well as different motif peptides, did not inhibit the P_O increase elicited by ATP.

There are probably two ways in which the cation channel and the kinase could be closely associated in excised patches. First, the channel and kinase could be directly bound to each other, or second, an intermediary scaffolding protein could bind both channel and kinase, thereby bringing the two molecules together. Moreover, the substrate of PKC need not be the channel itself but could be another component of the complex. The idea of a scaffolding protein is somewhat more attractive, given the number of components that potentially make up the signaling complex. Along with the PKC activity, two phosphatase activities are also present in excised, inside-out patches, namely, a serine-threonine phosphatase that is capable of reversing the effects of PKC and a tyrosine phosphatase that is activated by exogenous protein kinase A (PKA) (Wilson and Kaczmarek, 1993; Wilson et al., 1998). Furthermore, recent work suggests that a Ca²⁺- sensing mechanism involved in production of the refractory period into which the bag cell neurons enter after an afterdischarge may also be closely tethered to the cation channel (Magoski et al., 2000). The scaffolding protein hypothesis is also supported by the fact that the two PKC isoforms that have been cloned so far from Aplysia nervous tissue do not have apparent SH3 domains (Kruger et al., 1991). However, these data do not preclude the possibility that the channel itself may contain the SH3 domain, whereas the enzyme possesses an undefined binding motif.

There appears to be a high degree of specificity for both the src form of the SH3 domain and the src SH3 motif peptide over other SH3 domains and peptides in preventing the ATP response. This specificity may represent the possibility that src tyrosine kinase, in addition to its catalytic properties, also plays a scaffolding role in the cation channel-kinase protein complex. For example, Seibenhener et al. (1999) demonstrated that an atypical PKC isoform, PKC, binds to v-src tyrosine kinase. The role of src tyrosine kinase in the regulation of the cation channel is as yet unknown, however, although our preliminary attempts suggest that src tyrosine kinase inhibits P_O in the presence of ATP (Magoski and Kaczmarek, unpublished observation). Furthermore, we cannot completely exclude the possibility that the apparent specificity of the src SH3 domain represents its structural similarity to a native scaffolding protein.

There are examples of both direct and scaffolded enzyme-channel interactions. Src tyrosine kinase has been shown to closely associate with NMDA receptors (Yu et al., 1997) and Kv1.5 K⁺ channels (Holmes et al., 1996). Moreover, the src-Kv1.5 association was shown to be mediated by the direct interaction of the src SH3 domain and a polyproline SH3 motif on the K⁺ channel (Holmes et al., 1996). A well- studied example of a scaffolding protein is the multi-PDZ domain protein known as inaD (inactivation no-after potential D), which serves to assemble a signal transduction complex of phospholipase C, PKC, and the trp channel in Drosophila photoreceptors (Tsunoda et al., 1997; Tsunoda and Zuker, 1999). Another, more wide spread set of scaffolding proteins are cAMP-dependent protein kinase-anchoring proteins (AKAPs), some of which bring together several different phosphatases and kinases (Coghlan et al., 1995; Klauck et al., 1996). Peptide interference techniques similar to those used in the present study suggest that AKAPs participate in the PKA-dependent modulation of ionotropic glutamate receptors, Ca²⁺ channels, and Na⁺ channels, presumably by linking these channels with the kinase (Rosenmund et al., 1994; Johnson et al., 1997; Cantrell et al., 2000).

We also examined the potential role of the PKC-induced modulation of the cation channel in whole-cell recordings from isolated bag cell neurons. Cation channels were activated in cultured bag cell neurons by extracellular application of CtVm. The depolarizing response to CtVm in cultured bag cell neurons is attributable to activation of the cation channel and can be considered an in vitro analog of the afterdischarge itself (Wilson and Kaczmarek, 1993; Wilson et al., 1996; Magoski et al., 2000). We first sought to establish whether the CtVm-induced depolarization has a component that is dependent on PKC activation. The specific PKC pseudosubstrate inhibitor PKC_19-36 (House and Kemp, 1987), which has been shown previously to be very effective in preventing the increase in cation channel P_O elicited by ATP (Wilson et al., 1998), reduced the CtVm-induced depolarization by approximately one-third compared with controls. This level of reduction in response amplitude is in keeping with the fact that other pathways, including PKA, may be turned on by CtVm and contribute to cation channel activation and subsequent depolarization. There are data indicating that the cAMP/PKA pathway initiates a number of changes in bag cell neuron excitability during the afterdischarge (Kaczmarek et al., 1978, 1980; Kaczmarek and Strumwasser, 1981, 1984). Similarly, the CtVm-induced activation of the cation channel in cultured bag cell neurons involves, at least in part, a PKA-regulated pathway (Wilson and Kaczmarek, 1993). Thus, both the PKA and PKC pathways appear to act on the cation channel to enhance its activity and promote depolarization.

The CtVm response of cultured bag cell neurons was also attenuated by approximately one-third after dialysis with the SH3 domain from src but not the domain from yes. This suggests that, as in excised patches, modulation of the cation channel, as assayed by CtVm-induced depolarization, may involve SH3 domain-mediated protein-protein interactions. It should be noted that if additional enzymatic components of the signaling complex had also been affected by the src SH3 domain, it is likely that the inhibition of the CtVm-induced depolarization would have been larger than that observed with the PKC inhibitor. Overall, the results indicate not only that modulation of the cation channel requires specific protein-protein interactions, namely SH3-like interactions, to orchestrate channel modulation by PKC, but also that these interactions may play a part in cation channel-mediated depolarization of bag cell neurons. It would appear that signaling complexes are a common means by which ion channel modulation is carried out. This is supported by reports from the literature of regulatory enzymes being linked to ion channels (Holmes et al., 1996; Tsunoda et al., 1997; Yu et al., 1997; Tibbs et al., 1998; Davare et al., 2001) or of kinase modulation of ion channels persisting in cell-free system conditions (Rehm et al., 1989; Chung et al., 1991; Bielefeldt and Jackson, 1994; Yu et al., 1997; Wilson et al., 1998). For the bag cell neurons, it may very well be that a number of different protein-protein interactions are required to network the second messengers and enzymes that regulate their activation and initiation of the afterdischarge.

	FOOTNOTES

Received March 29, 2001; revised Sept. 17, 2001; accepted Sept. 18, 2001.

This work was supported by Human Frontiers Science Program and Medical Research Council of Canada postdoctoral fellowships to N.S.M. and by National Institutes of Health operating grants to G.F.W. and L.K.K. We are very grateful to Dr. B. K. Kay for providing interaction domains and peptides, Dr. B. M. Olivera for providing lyophilized Conus textile venom, and N. M. Magoski for critical evaluation of earlier drafts of this manuscript.

Correspondence should be addressed to Dr. L. K. Kaczmarek, Department of Pharmacology, Yale University School of Medicine, 333 Cedar Street, New Haven, CT 06520. E-mail: leonard.kaczmarek{at}.yale.edu.

	REFERENCES

TOP ABSTRACT INTRODUCTION MATERIALS AND METHODS RESULTS DISCUSSION REFERENCES

• Alonso A, Llinas RR (1989) Subthreshold Na⁺-dependent theta-like rhythmicity in stellate cells of entorhinal cortex layer II. Nature 342:175-177[Medline].
• Bielefeldt K, Jackson MB (1994) Phosphorylation and dephosphorylation modulate a Ca²⁺-activated K⁺ channel in rat peptidergic nerve terminals. J Physiol (Lond) 475:241-254[Abstract/Free Full Text].
• Cantrell AR, Tibbs VC, Westenbrock RE, Scheuer T, Catterall WA (2000) Dopaminergic modulation of voltage-gated Na⁺ current in rat hippocampal neurons requires anchoring of cAMP-dependent protein kinase. J Neurosci 19:RC21:1-6.
• Chung S, Reinhart PH, Martin BL, Brautigan D, Levitan IB (1991) Protein kinase activity closely associated with a reconstituted calcium-activated potassium channel. Science 253:560-562[Abstract/Free Full Text].
• Coghlan VM, Perrino BA, Howard M, Langeberg LK, Hicks JB, Gallatin WM, Scott JD (1995) Association of protein kinase A and protein phosphatase 2B with a common anchoring protein. Science 267:108-111[Abstract/Free Full Text].
• Cohen GB, Ren R, Baltimore D (1995) Modular binding domains in signal transduction proteins. Cell 80:237-248[Web of Science][Medline].
• Colquhoun D, Sigworth FJ (1995) Fitting and statistical analysis of single-channel records. In: Single channel recording (Sakmann B, Neher E, eds), pp 483-587. New York: Plenum.
• Conn PJ, Kaczmarek LK (1989) The bag cell neurons of Aplysia. Mol Neurobiol 3:237-273[Web of Science][Medline].
• Davare MA, Avdonin V, Hall DD, Peden EM, Burette A, Weinberg RJ, Horne MC, Hoshi T, Hell JW (2001) A ₂ adrenergic receptor signalling complex assembled with the Ca²⁺ channel Ca_V 1.2. Science 293:98-101[Abstract/Free Full Text].
• Fanning AS, Anderson JM (1999) Protein modules as organizers of membrane structure. Curr Opin Cell Biol 11:432-439[Web of Science][Medline].
• Fisher T, Levy S, Kaczmarek LK (1994) Transient changes in intracellular calcium associated with a prolonged increase in excitability in neurons of Aplysia californica. J Neurophysiol 71:1254-1257[Abstract/Free Full Text].
• Gee SH, Sekely SA, Lombardo C, Kurakin A, Froehner SC, Kay BK (1998) Cyclic peptides as non-carboxyl-terminal ligands of syntrophin PDZ domains. J Biol Chem 273:21980-21987[Abstract/Free Full Text].
• Green DJ, Gillette R (1983) Patch- and voltage-clamp analysis of cyclic AMP-stimulated inward current underlying neurone bursting. Nature 306:784-785[Medline].
• Holmes TC, Fadool DA, Ren R, Levitan IB (1996) Association of Src tyrosine kinase with a human potassium channel mediated by SH3 domain. Nature 274:2089-2091[Abstract/Free Full Text].
• House C, Kemp BE (1987) Protein kinase C contains a pseudosubstrate prototope in its regulatory domain. Science 238:1726-1728[Abstract/Free Full Text].
• Johnson BD, Brousal JP, Peterson BZ, Gallombardo PA, Hockerman GH, Lai Y, Scheuer T, Catterall WA (1997) Modulation of the cloned skeletal muscle L-type Ca²⁺ channel by anchored cAMP-dependent protein kinase. J Neurosci 17:1243-1255[Abstract/Free Full Text].
• Kaczmarek LK, Strumwasser F (1981) The expression of long lasting afterdischarge by isolated Aplysia bag cell neurons. J Neurosci 1:626-634[Web of Science][Medline].
• Kaczmarek LK, Strumwasser F (1984) A voltage-clamp analysis of currents underlying cyclic AMP-induced membrane modulation in isolated peptidergic neurons of Aplysia. J Neurophysiol 52:340-349[Abstract/Free Full Text].
• Kaczmarek LK, Jennings K, Strumwasser F (1978) Neurotransmitter modulation, phosphodiesterase inhibitor effects, and cyclic AMP correlates of afterdischarge in peptidergic neurites. Proc Natl Acad Sci USA 75:5200-5204[Abstract/Free Full Text].
• Kaczmarek LK, Jennings KR, Strumwasser F, Nairn AC, Walter U, Wilson FD, Greengard P (1980) Microinjection of catalytic subunit of cyclic AMP-dependent protein kinase enhances calcium action potentials of bag cell neurons in cell culture. Proc Natl Acad Sci USA 77:7487-7491[Abstract/Free Full Text].
• Kennedy MB (1995) Origin of PDZ (DHR, GLGF) domains. Trends Biochem Sci 20:350[Web of Science][Medline].
• Klauck TM, Faux MC, Labudda K, Langeberg LK, Jaken S, Scott JD (1996) Coordination of three signalling enzymes by AKAP79, a mammalian scaffold protein. Science 271:1589-1592[Abstract].
• Knox RJ, Quattrocki EA, Connor JA, Kaczmarek LK (1992) Recruitment of Ca²⁺ channels by protein kinase C during rapid formation of putative neuropeptide release sites in isolated Aplysia neurons. Neuron 8:883-889[Web of Science][Medline].
• Knox RJ, Jonas EA, Kao L-S, Smith PJS, Connor JA, Kaczmarek LK (1996) Ca²⁺ influx and activation of a cation current are coupled to intracellular Ca²⁺ release in peptidergic neurons of Aplysia californica. J Physiol (Lond) 494:627-693[Abstract/Free Full Text].
• Koch CA, Anderson D, Moran MF, Ellis C, Pawson T (1991) SH2 and SH3 domains: elements that control interactions of cytoplasmic signalling proteins. Science 252:68-674[Abstract/Free Full Text].
• Kruger KE, Sossin WS, Sacktor TC, Bergold PJ, Beushausen S, Schwartz JH (1991) Cloning and characterization of Ca²⁺-dependent and Ca²⁺-independent PKCs expressed in Aplysia sensory cells. J Neurosci 11:2303-2313[Abstract].
• Kupfermann I (1967) Stimulation of egg laying: possible neuroendocrine function of bag cells of abdominal ganglion of Aplysia californica. Nature 216:814-815[Medline].
• Kupfermann I, Kandel ER (1970) Electrophysiological properties and functional interconnections of two symmetrical neurosecretory clusters (bag cells) in abdominal ganglion of Aplysia. J Neurophysiol 33:865-876[Free Full Text].
• Levitan IB, Kaczmarek LK (1997) In: The neuron: cell and molecular biology. New York: Oxford UP.
• Magoski NS, Knox RJ, Kaczmarek LK (2000) Activation of a Ca²⁺-permeable cation channel produces a prolonged attenuation of intracellular Ca²⁺ release in Aplysia bag cell neurones. J Physiol (Lond) 522:271-283[Abstract/Free Full Text].
• Nguyen JT, Turck CW, Cohen FE, Zuckermann RN, Lim WA (1998) Exploiting the basis of proline recognition by SH3 and WW domains: design of N-substituted inhibitors. Science 282:2088-2092[Abstract/Free Full Text].
• Olivera BM, River J, Clark C, Ramilo CA, Corpuz GP, Abogadie FC, Mena EE, Woodward SR, Hillyard DR, Cruz LJ (1990) Diversity of Conus neuropeptides. Science 249:257-263[Abstract/Free Full Text].
• Pawson T (1995) Protein modules and signalling networks. Nature 373:573-579[Medline].
• Pawson T, Scott JD (1997) Signalling through scaffold, anchoring, and adaptor proteins. Science 278:2075-2080[Abstract/Free Full Text].
• Pinsker HM, Dudek FE (1977) Bag cell control of egg laying in freely behaving Aplysia. Science 197:490-493[Abstract/Free Full Text].
• Pirozzi G, McConnell SJ, Uveges AJ, Carter JM, Sparks AB, Kay BK, Fowlkes DM (1997) Identification of novel human WW domain-containing proteins by cloning of ligand targets. J Biol Chem 272:14611-14616[Abstract/Free Full Text].
• Rehm H, Pelzer S, Cochet C, Chambaz E, Tempel BL, Trautwein W, Pelzer D, Lazdunski M (1989) Dendrotoxin-binding brain membrane protein displays a K⁺ channel activity that is stimulated by both cAMP-dependent and endogenous phosphorylations. Biochemistry 28:6455-6460[Medline].
• Rosenmund C, Carr DW, Bergeson SE, Nilaver G, Scott JD, Westbrook GL (1994) Anchoring of protein kinase A is required for modulation of AMPA/kainate receptors on hippocampal neurons. Nature 368:853-856[Medline].
• Rothman BS, Weir G, Dudek FE (1983) Egg-laying hormone: direct action on the ovotestis of Aplysia. Gen Comp Endocrinol 52:134-141[Web of Science][Medline].
• Seibenhener ML, Roehm J, White WO, Neidigh KB, Vandenplas ML, Wooten MW (1999) Identification of src as a novel atypical protein kinase C-interacting protein. Mol Cell Biol Res Commun 2:28-31[Medline].
• Sheng M, Wyszynski M (1997) Ion channel targeting in neurons. BioEssays 19:847-853[Web of Science][Medline].
• Sparks AB, Rider JE, Hoffman NG, Fowlkes DM, Quillan LA, Kay BK (1996) Distinct ligand preferences of src homology 3 domains from src, yes, abl, cortactin, p53bp2, PLCgamma, crk, and grb2. Proc Natl Acad Sci USA 93:1540-1544[Abstract/Free Full Text].
• Stafstrom CE, Schwindt PC, Chubb MC, Crill WE (1985) Properties of persistent sodium conductance and calcium conductance of layer V neurons from cat sensorimotor cortex in vitro. J Neurophysiol 53:153-170[Abstract/Free Full Text].
• Staub O, Rotin D (1997) Regulation of ion transport by protein-protein interaction domains. Curr Opin Nephrol Hypertens 6:447-454[Web of Science][Medline].
• Strong JA, Fox AP, Tsien RW, Kaczmarek LK (1987) Stimulation of protein kinase C recruits covert calcium channels in Aplysia bag cell neurons. Nature 325:714-717[Medline].
• Swandulla D, Lux HD (1985) Activation of a nonspecific cation conductance by intracellular Ca²⁺ elevation in bursting pacemaker neurons of Helix pomatia. J Neurophysiol 54:1430-1443[Abstract/Free Full Text].
• Tibbs VC, Gray PC, Catterall WA, Murphy BJ (1998) AKAP15 anchors cAMP-dependent protein kinase to brain sodium channels. J Biol Chem 273:25783-25788[Abstract/Free Full Text].
• Tsunoda S, Zuker CS (1999) The organization of INAD-signalling complexes by a multivalent PDZ domain protein in Drosophila photoreceptor cells ensures sensitivity and speed of signalling. Cell Calcium 26:165-171[Web of Science][Medline].
• Tsunoda S, Sierralta J, Sun Y, Bodner R, Suzuki E, Becker A, Socolich M, Zuker CS (1997) A multivalent PDZ-domain protein assembles signalling complexes in a G-protein-coupled cascade. Nature 388:243-249[Web of Science][Medline].
• Wayne NL, Lee W, Kim YJ (1999) Persistent activation of calcium-activated and calcium-independent protein kinase C in response to electrical afterdischarge from peptidergic neurons of Aplysia. Brain Res 834:211-213[Web of Science][Medline].
• Wilson GF, Kaczmarek LK (1993) Mode-switching of a voltage-gated cation channel is mediated by a protein kinase A-regulated tyrosine phosphatase. Nature 366:433-438[Medline].
• Wilson GF, Richardson FC, Fisher TE, Olivera BM, Kaczmarek LK (1996) Identification and characterization of a Ca²⁺-sensitive nonspecific cation channel underlying prolonged repetitive firing in Aplysia neurons. J Neurosci 16:3661-3671[Abstract/Free Full Text].
• Wilson GF, Magoski NS, Kaczmarek LK (1998) Modulation of a calcium-sensitive nonspecific cation channel by ATP via a closely-associated protein kinase. Proc Natl Acad Sci USA 95:10938-10943[Abstract/Free Full Text].
• Wilson WA, Wachtel H (1974) Negative resistance characteristic essential for the maintenance of slow oscillations in bursting neurons. Science 186:932-934[Abstract/Free Full Text].
• Yu X-M, Askalan R, Keil GJ, Salter MW (1997) NMDA channel regulation by channel-associated protein tyrosine kinase src. Science 275:674-678[Abstract/Free Full Text].

---

Copyright © 2002 Society for Neuroscience  0270-6474/02/2211-09$05.00/0

This article has been cited by other articles:

				A. Evrard and N. Ropert Early Development of the Thalamic Inhibitory Feedback Loop in the Primary Somatosensory System of the Newborn Mice J. Neurosci., August 5, 2009; 29(31): 9930 - 9940. [Abstract] [Full Text] [PDF]

				K. E. Gardam and N. S. Magoski Regulation of Cation Channel Voltage and Ca2+ Dependence by Multiple Modulators J Neurophysiol, July 1, 2009; 102(1): 259 - 271. [Abstract] [Full Text] [PDF]

				L. de Almeida, M. Idiart, and J. E. Lisman A Second Function of Gamma Frequency Oscillations: An E%-Max Winner-Take-All Mechanism Selects Which Cells Fire J. Neurosci., June 10, 2009; 29(23): 7497 - 7503. [Abstract] [Full Text] [PDF]

				D. R. Marks, K. Tucker, M. A. Cavallin, T. G. Mast, and D. A. Fadool Awake Intranasal Insulin Delivery Modifies Protein Complexes and Alters Memory, Anxiety, and Olfactory Behaviors J. Neurosci., May 20, 2009; 29(20): 6734 - 6751. [Abstract] [Full Text] [PDF]

				T. Furuta, T. Kaneko, and M. Deschenes Septal Neurons in Barrel Cortex Derive Their Receptive Field Input from the Lemniscal Pathway J. Neurosci., April 1, 2009; 29(13): 4089 - 4095. [Abstract] [Full Text] [PDF]

				H.-K. Lee, P. Kumar, Q. Fu, K. M. Rosen, and H. W. Querfurth The Insulin/Akt Signaling Pathway Is Targeted by Intracellular {beta}-Amyloid Mol. Biol. Cell, March 1, 2009; 20(5): 1533 - 1544. [Abstract] [Full Text] [PDF]

				K. L. Lambertsen, B. H. Clausen, A. A. Babcock, R. Gregersen, C. Fenger, H. H. Nielsen, L. S. Haugaard, M. Wirenfeldt, M. Nielsen, F. Dagnaes-Hansen, et al. Microglia Protect Neurons against Ischemia by Synthesis of Tumor Necrosis Factor J. Neurosci., February 4, 2009; 29(5): 1319 - 1330. [Abstract] [Full Text] [PDF]

				A. Khoutorsky and M. E. Spira Activity-dependent calpain activation plays a critical role in synaptic facilitation and post-tetanic potentiation Learn. Mem., January 29, 2009; 16(2): 129 - 141. [Abstract] [Full Text] [PDF]

				M. Bal, J. Zhang, O. Zaika, C. C. Hernandez, and M. S. Shapiro Homomeric and Heteromeric Assembly of KCNQ (Kv7) K+ Channels Assayed by Total Internal Reflection Fluorescence/Fluorescence Resonance Energy Transfer and Patch Clamp Analysis J. Biol. Chem., November 7, 2008; 283(45): 30668 - 30676. [Abstract] [Full Text] [PDF]

				P.-P. Lenck-Santini, A. A. Fenton, and R. U. Muller Discharge Properties of Hippocampal Neurons during Performance of a Jump Avoidance Task J. Neurosci., July 2, 2008; 28(27): 6773 - 6786. [Abstract] [Full Text] [PDF]

				S. L. Prime, M. Vesia, and J. D. Crawford Transcranial Magnetic Stimulation over Posterior Parietal Cortex Disrupts Transsaccadic Memory of Multiple Objects J. Neurosci., July 2, 2008; 28(27): 6938 - 6949. [Abstract] [Full Text] [PDF]

				K. E. Gardam, J. E. Geiger, C. M. Hickey, A. Y. Hung, and N. S. Magoski Flufenamic Acid Affects Multiple Currents and Causes Intracellular Ca2+ Release in Aplysia Bag Cell Neurons J Neurophysiol, July 1, 2008; 100(1): 38 - 49. [Abstract] [Full Text] [PDF]

				J. E. Geiger and N. S. Magoski Ca2+-Induced Ca2+ Release in Aplysia Bag Cell Neurons Requires Interaction Between Mitochondrial and Endoplasmic Reticulum Stores J Neurophysiol, July 1, 2008; 100(1): 24 - 37. [Abstract] [Full Text] [PDF]

				H. Bokor, L. Acsady, and M. Deschenes Vibrissal Responses of Thalamic Cells That Project to the Septal Columns of the Barrel Cortex and to the Second Somatosensory Area J. Neurosci., May 14, 2008; 28(20): 5169 - 5177. [Abstract] [Full Text] [PDF]

				S. Hirano, Y. Hirano, T. Maekawa, C. Obayashi, N. Oribe, T. Kuroki, S. Kanba, and T. Onitsuka Abnormal Neural Oscillatory Activity to Speech Sounds in Schizophrenia: A Magnetoencephalography Study J. Neurosci., May 7, 2008; 28(19): 4897 - 4903. [Abstract] [Full Text] [PDF]

				M. Son, S. C. Leary, N. Romain, F. Pierrel, D. R. Winge, R. G. Haller, and J. L. Elliott Isolated Cytochrome c Oxidase Deficiency in G93A SOD1 Mice Overexpressing CCS Protein J. Biol. Chem., May 2, 2008; 283(18): 12267 - 12275. [Abstract] [Full Text] [PDF]

				P. Cassina, A. Cassina, M. Pehar, R. Castellanos, M. Gandelman, A. de Leon, K. M. Robinson, R. P. Mason, J. S. Beckman, L. Barbeito, et al. Mitochondrial Dysfunction in SOD1G93A-Bearing Astrocytes Promotes Motor Neuron Degeneration: Prevention by Mitochondrial-Targeted Antioxidants J. Neurosci., April 16, 2008; 28(16): 4115 - 4122. [Abstract] [Full Text] [PDF]

				A. Alink, W. Singer, and L. Muckli Capture of Auditory Motion by Vision Is Represented by an Activation Shift from Auditory to Visual Motion Cortex J. Neurosci., March 12, 2008; 28(11): 2690 - 2697. [Abstract] [Full Text] [PDF]

				R.-Y. Liu, D. Fioravante, S. Shah, and J. H. Byrne cAMP Response Element-Binding Protein 1 Feedback Loop Is Necessary for Consolidation of Long-Term Synaptic Facilitation in Aplysia J. Neurosci., February 20, 2008; 28(8): 1970 - 1976. [Abstract] [Full Text] [PDF]

				K. K. Ade, M. J. Janssen, P. I. Ortinski, and S. Vicini Differential Tonic GABA Conductances in Striatal Medium Spiny Neurons J. Neurosci., January 30, 2008; 28(5): 1185 - 1197. [Abstract] [Full Text] [PDF]

				C. D. Derby Escape by Inking and Secreting: Marine Molluscs Avoid Predators Through a Rich Array of Chemicals and Mechanisms Biol. Bull., December 1, 2007; 213(3): 274 - 289. [Abstract] [Full Text] [PDF]

				F. F. Trigo, M. Chat, and A. Marty Enhancement of GABA Release through Endogenous Activation of Axonal GABAA Receptors in Juvenile Cerebellum J. Neurosci., November 14, 2007; 27(46): 12452 - 12463. [Abstract] [Full Text] [PDF]

				J. Liang, A. Suryanarayanan, A. Abriam, B. Snyder, R. W. Olsen, and I. Spigelman Mechanisms of Reversible GABAA Receptor Plasticity after Ethanol Intoxication J. Neurosci., November 7, 2007; 27(45): 12367 - 12377. [Abstract] [Full Text] [PDF]

				N. Urbain and M. Deschenes A New Thalamic Pathway of Vibrissal Information Modulated by the Motor Cortex J. Neurosci., November 7, 2007; 27(45): 12407 - 12412. [Abstract] [Full Text] [PDF]

				S. Kuriki, K. Ohta, and S. Koyama Persistent Responsiveness of Long-Latency Auditory Cortical Activities in Response to Repeated Stimuli of Musical Timbre and Vowel Sounds Cereb Cortex, November 1, 2007; 17(11): 2725 - 2732. [Abstract] [Full Text] [PDF]

				M. Mongiat, G. Ligresti, S. Marastoni, E. Lorenzon, R. Doliana, and A. Colombatti Regulation of the Extrinsic Apoptotic Pathway by the Extracellular Matrix Glycoprotein EMILIN2 Mol. Cell. Biol., October 15, 2007; 27(20): 7176 - 7187. [Abstract] [Full Text] [PDF]

				R. P. Bonin, L. J. Martin, J. F. MacDonald, and B. A. Orser {alpha}5GABAA Receptors Regulate the Intrinsic Excitability of Mouse Hippocampal Pyramidal Neurons J Neurophysiol, October 1, 2007; 98(4): 2244 - 2254. [Abstract] [Full Text] [PDF]

				J. Ostergaard, J. Hannibal, and J. Fahrenkrug Synaptic Contact between Melanopsin-Containing Retinal Ganglion Cells and Rod Bipolar Cells Invest. Ophthalmol. Vis. Sci., August 1, 2007; 48(8): 3812 - 3820. [Abstract] [Full Text] [PDF]

				M. Sun, M. J. Thomas, R. Herder, M. L. Bofenkamp, S. B. Selleck, and M. B. O'Connor Presynaptic Contributions of Chordin to Hippocampal Plasticity and Spatial Learning J. Neurosci., July 18, 2007; 27(29): 7740 - 7750. [Abstract] [Full Text] [PDF]

				Y. Yan, K. Yamada, M. Niwa, T. Nagai, A. Nitta, and T. Nabeshima Enduring vulnerability to reinstatement of methamphetamine-seeking behavior in glial cell line-derived neurotrophic factor mutant mice FASEB J, July 1, 2007; 21(9): 1994 - 2004. [Abstract] [Full Text] [PDF]

				M. R. Regan, Y. H. Huang, Y. S. Kim, M. I. Dykes-Hoberg, L. Jin, A. M. Watkins, D. E. Bergles, and J. D. Rothstein Variations in Promoter Activity Reveal a Differential Expression and Physiology of Glutamate Transporters by Glia in the Developing and Mature CNS J. Neurosci., June 20, 2007; 27(25): 6607 - 6619. [Abstract] [Full Text] [PDF]

				E. Taoufik, S. Valable, G. J. Muller, M. L. Roberts, D. Divoux, A. Tinel, A. Voulgari-Kokota, V. Tseveleki, F. Altruda, H. Lassmann, et al. FLIPL Protects Neurons against In Vivo Ischemia and In Vitro Glucose Deprivation-Induced Cell Death J. Neurosci., June 20, 2007; 27(25): 6633 - 6646. [Abstract] [Full Text] [PDF]

				H. Okamoto, Y. Isomura, M. Takada, and T. Fukai Temporal Integration by Stochastic Recurrent Network Dynamics With Bimodal Neurons J Neurophysiol, June 1, 2007; 97(6): 3859 - 3867. [Abstract] [Full Text] [PDF]

				C. Geisler, D. Robbe, M. Zugaro, A. Sirota, and G. Buzsaki Hippocampal place cell assemblies are speed-controlled oscillators PNAS, May 8, 2007; 104(19): 8149 - 8154. [Abstract] [Full Text] [PDF]

				M. V. Soldovieri, M. R. Cilio, F. Miceli, G. Bellini, E. Miraglia del Giudice, P. Castaldo, C. C. Hernandez, M. S. Shapiro, A. Pascotto, L. Annunziato, et al. Atypical Gating Of M-Type Potassium Channels Conferred by Mutations in Uncharged Residues in the S4 Region of KCNQ2 Causing Benign Familial Neonatal Convulsions J. Neurosci., May 2, 2007; 27(18): 4919 - 4928. [Abstract] [Full Text] [PDF]

				M. Son, K. Puttaparthi, H. Kawamata, B. Rajendran, P. J. Boyer, G. Manfredi, and J. L. Elliott Overexpression of CCS in G93A-SOD1 mice leads to accelerated neurological deficits with severe mitochondrial pathology PNAS, April 3, 2007; 104(14): 6072 - 6077. [Abstract] [Full Text] [PDF]

				A. Y. Hung and N. S. Magoski Activity-Dependent Initiation of a Prolonged Depolarization in Aplysia Bag Cell Neurons: Role for a Cation Channel J Neurophysiol, March 1, 2007; 97(3): 2465 - 2479. [Abstract] [Full Text] [PDF]

				V. Zsiros, I. Aradi, and G. Maccaferri Propagation of postsynaptic currents and potentials via gap junctions in GABAergic networks of the rat hippocampus J. Physiol., January 15, 2007; 578(2): 527 - 544. [Abstract] [Full Text] [PDF]

				C. A. Czeisler and J. J. Gooley Sleep and Circadian Rhythms in Humans Cold Spring Harb Symp Quant Biol, January 1, 2007; 72(0): 579 - 597. [Abstract] [PDF]

				M. Wei, N. Li, S. D. Newlands, J. D. Dickman, and D. E. Angelaki Deficits and Recovery in Visuospatial Memory During Head Motion After Bilateral Labyrinthine Lesion J Neurophysiol, September 1, 2006; 96(3): 1676 - 1682. [Abstract] [Full Text] [PDF]

				D. A. Lupinsky and N. S. Magoski Ca2+-dependent regulation of a non-selective cation channel from Aplysia bag cell neurones J. Physiol., September 1, 2006; 575(2): 491 - 506. [Abstract] [Full Text] [PDF]

				H. Nishimaru, C. E. Restrepo, and O. Kiehn Activity of Renshaw cells during locomotor-like rhythmic activity in the isolated spinal cord of neonatal mice. J. Neurosci., May 17, 2006; 26(20): 5320 - 5328. [Abstract] [Full Text] [PDF]

				J. Chin, R.-Y. Liu, L. J. Cleary, A. Eskin, and J. H. Byrne TGF-beta1-Induced Long-Term Changes in Neuronal Excitability in Aplysia Sensory Neurons Depend on MAPK J Neurophysiol, May 1, 2006; 95(5): 3286 - 3290. [Abstract] [Full Text] [PDF]

				J. H. Song, A. Bellail, M. C. L. Tse, V. W. Yong, and C. Hao Human astrocytes are resistant to Fas ligand and tumor necrosis factor-related apoptosis-inducing ligand-induced apoptosis. J. Neurosci., March 22, 2006; 26(12): 3299 - 3308. [Abstract] [Full Text] [PDF]

				J. F. Otto, Y. Yang, W. N. Frankel, H. S. White, and K. S. Wilcox A Spontaneous Mutation Involving Kcnq2 (Kv7.2) Reduces M-Current Density and Spike Frequency Adaptation in Mouse CA1 Neurons J. Neurosci., February 15, 2006; 26(7): 2053 - 2059. [Abstract] [Full Text] [PDF]

				S. Reddy Kinases Bicker Over an Ion Channel J. Neurosci., February 8, 2006; 26(6): 1657 - 1658. [Full Text] [PDF]

				Z. Mtchedlishvili and J. Kapur High-Affinity, Slowly Desensitizing GABAA Receptors Mediate Tonic Inhibition in Hippocampal Dentate Granule Cells Mol. Pharmacol., February 1, 2006; 69(2): 564 - 575. [Abstract] [Full Text] [PDF]

				E. Azim, D. Mobbs, B. Jo, V. Menon, and A. L. Reiss Sex differences in brain activation elicited by humor PNAS, November 8, 2005; 102(45): 16496 - 16501. [Abstract] [Full Text] [PDF]

				S. A. Kushner, Y. Elgersma, G. G. Murphy, D. Jaarsma, G. M. van Woerden, M. R. Hojjati, Y. Cui, J. C. LeBoutillier, D. F. Marrone, E. S. Choi, et al. Modulation of Presynaptic Plasticity and Learning by the H-ras/Extracellular Signal-Regulated Kinase/Synapsin I Signaling Pathway J. Neurosci., October 19, 2005; 25(42): 9721 - 9734. [Abstract] [Full Text] [PDF]

				N. S. Magoski and L. K. Kaczmarek Association/Dissociation of a Channel-Kinase Complex Underlies State-Dependent Modulation J. Neurosci., August 31, 2005; 25(35): 8037 - 8047. [Abstract] [Full Text] [PDF]

				C. Fuchs, V. Forster, E. Balse, J.-A. Sahel, S. Picaud, and L.-H. Tessier Retinal-Cell-Conditioned Medium Prevents TNF-{alpha}-Induced Apoptosis of Purified Ganglion Cells Invest. Ophthalmol. Vis. Sci., August 1, 2005; 46(8): 2983 - 2991. [Abstract] [Full Text] [PDF]

				G. C. Brainard and J. P. Hanifin Photons, Clocks, and Consciousness J Biol Rhythms, August 1, 2005; 20(4): 314 - 325. [Abstract] [PDF]

				G. A. Phares and J. H. Byrne Analysis of 5-HT-Induced Short-Term Facilitation at Aplysia Sensorimotor Synapse During Bursts: Increased Synaptic Gain That Does Not Require ERK Activation J Neurophysiol, July 1, 2005; 94(1): 871 - 877. [Abstract] [Full Text] [PDF]

				H. Tummala, C. Jung, A. Tiwari, C. M. J. Higgins, L. J. Hayward, and Z. Xu Inhibition of Chaperone Activity Is a Shared Property of Several Cu,Zn-Superoxide Dismutase Mutants That Cause Amyotrophic Lateral Sclerosis J. Biol. Chem., May 6, 2005; 280(18): 17725 - 17731. [Abstract] [Full Text] [PDF]

				C.-B. Zhu, A. M. Carneiro, W. R. Dostmann, W. A. Hewlett, and R. D. Blakely p38 MAPK Activation Elevates Serotonin Transport Activity via a Trafficking-independent, Protein Phosphatase 2A-dependent Process J. Biol. Chem., April 22, 2005; 280(16): 15649 - 15658. [Abstract] [Full Text] [PDF]

				P. S. Mangan, C. Sun, M. Carpenter, H. P. Goodkin, W. Sieghart, and J. Kapur Cultured Hippocampal Pyramidal Neurons Express Two Kinds of GABAA Receptors Mol. Pharmacol., March 1, 2005; 67(3): 775 - 788. [Abstract] [Full Text] [PDF]

				Y.-Q. Cao and R. W. Tsien Effects of familial hemiplegic migraine type 1 mutations on neuronal P/Q-type Ca2+ channel activity and inhibitory synaptic transmission PNAS, February 15, 2005; 102(7): 2590 - 2595. [Abstract] [Full Text] [PDF]

				M. Ruger, M. C. M. Gordijn, D. G. M. Beersma, B. de Vries, and S. Daan Nasal versus Temporal Illumination of the Human Retina: Effects on Core Body Temperature, Melatonin, and Circadian Phase J Biol Rhythms, February 1, 2005; 20(1): 60 - 70. [Abstract] [PDF]

				J. Yan, Y. Zhang, and G. Ehret Corticofugal Shaping of Frequency Tuning Curves in the Central Nucleus of the Inferior Colliculus of Mice J Neurophysiol, January 1, 2005; 93(1): 71 - 83. [Abstract] [Full Text] [PDF]

				D. A. Wilson Odor Perception is Dynamic: Consequences for Interpretation of Odor Maps Chem Senses, January 1, 2005; 30(suppl_1): i105 - i106. [Full Text] [PDF]

				K. M. O'Brien, R. Dirmeier, M. Engle, and R. O. Poyton Mitochondrial Protein Oxidation in Yeast Mutants Lacking Manganese-(MnSOD) or Copper- and Zinc-containing Superoxide Dismutase (CuZnSOD): EVIDENCE THAT MnSOD AND CuZnSOD HAVE BOTH UNIQUE AND OVERLAPPING FUNCTIONS IN PROTECTING MITOCHONDRIAL PROTEINS FROM OXIDATIVE DAMAGE J. Biol. Chem., December 10, 2004; 279(50): 51817 - 51827. [Abstract] [Full Text] [PDF]

				V. Khatri, J. A. Hartings, and D. J. Simons Adaptation in Thalamic Barreloid and Cortical Barrel Neurons to Periodic Whisker Deflections Varying in Frequency and Velocity J Neurophysiol, December 1, 2004; 92(6): 3244 - 3254. [Abstract] [Full Text] [PDF]

				G. Tezel and X. Yang Caspase-Independent Component of Retinal Ganglion Cell Death, In Vitro Invest. Ophthalmol. Vis. Sci., November 1, 2004; 45(11): 4049 - 4059. [Abstract] [Full Text] [PDF]

				J. Hannibal, P. Hindersson, J. Ostergaard, B. Georg, S. Heegaard, P. J. Larsen, and J. Fahrenkrug Melanopsin Is Expressed in PACAP-Containing Retinal Ganglion Cells of the Human Retinohypothalamic Tract Invest. Ophthalmol. Vis. Sci., November 1, 2004; 45(11): 4202 - 4209. [Abstract] [Full Text] [PDF]

				A. K. Tryba and J.-M. Ramirez Hyperthermia Modulates Respiratory Pacemaker Bursting Properties J Neurophysiol, November 1, 2004; 92(5): 2844 - 2852. [Abstract] [Full Text] [PDF]

				L. de Yebra, R. Adroer, N. de Gregorio-Rocasolano, R. Blesa, R. Trullas, and N. Mahy Reduced KIAA0471 mRNA expression in Alzheimer's patients: a new candidate gene product linked to the disease? Hum. Mol. Genet., November 1, 2004; 13(21): 2607 - 2612. [Abstract] [Full Text] [PDF]

				E. M. Petrini, I. Marchionni, P. Zacchi, W. Sieghart, and E. Cherubini Clustering of Extrasynaptic GABAA Receptors Modulates Tonic Inhibition in Cultured Hippocampal Neurons J. Biol. Chem., October 29, 2004; 279(44): 45833 - 45843. [Abstract] [Full Text] [PDF]

				L. Marchetti, M. Klein, K. Schlett, K. Pfizenmaier, and U. L. M. Eisel Tumor Necrosis Factor (TNF)-mediated Neuroprotection against Glutamate-induced Excitotoxicity Is Enhanced by N-Methyl-D-aspartate Receptor Activation: ESSENTIAL ROLE OF A TNF RECEPTOR 2-MEDIATED PHOSPHATIDYLINOSITOL 3-KINASE-DEPENDENT NF-{kappa}B PATHWAY J. Biol. Chem., July 30, 2004; 279(31): 32869 - 32881. [Abstract] [Full Text] [PDF]

				N. S. Magoski Regulation of an Aplysia Bag Cell Neuron Cation Channel by Closely Associated Protein Kinase A and a Protein Phosphatase J. Neurosci., July 28, 2004; 24(30): 6833 - 6841. [Abstract] [Full Text] [PDF]

				D. Ait-Ali, V. Turquier, L. Grumolato, L. Yon, M. Jourdain, D. Alexandre, L. E. Eiden, H. Vaudry, and Y. Anouar The Proinflammatory Cytokines Tumor Necrosis Factor-{alpha} and Interleukin-1 Stimulate Neuropeptide Gene Transcription and Secretion in Adrenochromaffin Cells via Activation of Extracellularly Regulated Kinase 1/2 and p38 Protein Kinases, and Activator Protein-1 Transcription Factors Mol. Endocrinol., July 1, 2004; 18(7): 1721 - 1739. [Abstract] [Full Text] [PDF]

				E. A. Budygin, M. S. Brodie, T. D. Sotnikova, Y. Mateo, C. E. John, M. Cyr, R. R. Gainetdinov, and S. R. Jones Dissociation of rewarding and dopamine transporter-mediated properties of amphetamine PNAS, May 18, 2004; 101(20): 7781 - 7786. [Abstract] [Full Text] [PDF]

				K. E. Cullen and J. E. Roy Signal Processing in the Vestibular System During Active Versus Passive Head Movements J Neurophysiol, May 1, 2004; 91(5): 1919 - 1933. [Abstract] [Full Text] [PDF]

				N. Schmitzer-Torbert and A. D. Redish Neuronal Activity in the Rodent Dorsal Striatum in Sequential Navigation: Separation of Spatial and Reward Responses on the Multiple T Task J Neurophysiol, May 1, 2004; 91(5): 2259 - 2272. [Abstract] [Full Text] [PDF]

				Q. Li, S. Guo-Ross, D. V. Lewis, D. Turner, A. M. White, W. A. Wilson, and H. S. Swartzwelder Dietary Prenatal Choline Supplementation Alters Postnatal Hippocampal Structure and Function J Neurophysiol, April 1, 2004; 91(4): 1545 - 1555. [Abstract] [Full Text] [PDF]

				V. B. Caraiscos, E. M. Elliott, K. E. You-Ten, V. Y. Cheng, D. Belelli, J. G. Newell, M. F. Jackson, J. J. Lambert, T. W. Rosahl, K. A. Wafford, et al. Tonic inhibition in mouse hippocampal CA1 pyramidal neurons is mediated by {alpha}5 subunit-containing {gamma}-aminobutyric acid type A receptors PNAS, March 9, 2004; 101(10): 3662 - 3667. [Abstract] [Full Text] [PDF]

				M. A. Sommer and R. H. Wurtz What the Brain Stem Tells the Frontal Cortex. II. Role of the SC-MD-FEF Pathway in Corollary Discharge J Neurophysiol, March 1, 2004; 91(3): 1403 - 1423. [Abstract] [Full Text] [PDF]

				R. M. Robertson Modulation of Neural Circuit Operation by Prior Environmental Stress Integr. Comp. Biol., February 1, 2004; 44(1): 21 - 27. [Abstract] [Full Text] [PDF]

				T. Suzuki, I. Hide, K. Ido, S. Kohsaka, K. Inoue, and Y. Nakata Production and Release of Neuroprotective Tumor Necrosis Factor by P2X7 Receptor-Activated Microglia J. Neurosci., January 7, 2004; 24(1): 1 - 7. [Abstract] [Full Text] [PDF]

				A. D. Patel and E. Balaban Human Auditory Cortical Dynamics During Perception of Long Acoustic Sequences: Phase Tracking of Carrier Frequency by the Auditory Steady-state Response Cereb Cortex, January 1, 2004; 14(1): 35 - 46. [Abstract] [Full Text] [PDF]

				B. S. Minnery, R. M. Bruno, and D. J. Simons Response Transformation and Receptive-Field Synthesis in the Lemniscal Trigeminothalamic Circuit J Neurophysiol, September 1, 2003; 90(3): 1556 - 1570. [Abstract] [Full Text] [PDF]

				A. J. Gruber, S. A. Solla, D. J. Surmeier, and J. C. Houk Modulation of Striatal Single Units by Expected Reward: A Spiny Neuron Model Displaying Dopamine-Induced Bistability J Neurophysiol, August 1, 2003; 90(2): 1095 - 1114. [Abstract] [Full Text] [PDF]

				L. Vila, E. F Barrett, and J. N Barrett Stimulation-induced mitochondrial [Ca2+] elevations in mouse motor terminals: comparison of wild-type with SOD1-G93A J. Physiol., June 15, 2003; 549(3): 719 - 728. [Abstract] [Full Text] [PDF]

				A. K. Tryba and J.-M. Ramirez Response of the Respiratory Network of Mice to Hyperthermia J Neurophysiol, June 1, 2003; 89(6): 2975 - 2983. [Abstract] [Full Text] [PDF]

				I. Strauss and A. Lev-Tov Neural Pathways Between Sacrocaudal Afferents and Lumbar Pattern Generators in Neonatal Rats J Neurophysiol, February 1, 2003; 89(2): 773 - 784. [Abstract] [Full Text] [PDF]

				G. Glickman, J. P. Hanifin, M. D. Rollag, J. Wang, H. Cooper, and G. C. Brainard Inferior Retinal Light Exposure Is More Effective than Superior Retinal Exposure in Suppressing Melatonin in Humans J Biol Rhythms, February 1, 2003; 18(1): 71 - 79. [Abstract] [PDF]

				T. Yoshimura, Y. Yokota, A. Ishikawa, S. Yasuo, N. Hayashi, T. Suzuki, N. Okabayashi, T. Namikawa, and S. Ebihara Mapping Quantitative Trait Loci Affecting Circadian Photosensitivity in Retinally Degenerate Mice J Biol Rhythms, December 1, 2002; 17(6): 512 - 519. [Abstract] [PDF]

				C. J. Quintans, M. J. Donaldson, M. V. Bertolino, and R. S. Pasqualini Birth of two babies using oocytes that were cryopreserved in a choline-based freezing medium Hum. Reprod., December 1, 2002; 17(12): 3149 - 3152. [Abstract] [Full Text] [PDF]

				Y. Zhang, N. S. Magoski, and L. K. Kaczmarek Prolonged Activation of Ca2+-Activated K+ Current Contributes to the Long-Lasting Refractory Period of Aplysia Bag Cell Neurons J. Neurosci., December 1, 2002; 22(23): 10134 - 10141. [Abstract] [Full Text] [PDF]

				P. M. Baker, P. S. Pennefather, B. A. Orser, and F. K. Skinner Disruption of Coherent Oscillations in Inhibitory Networks With Anesthetics: Role of GABAA Receptor Desensitization J Neurophysiol, November 1, 2002; 88(5): 2821 - 2833. [Abstract] [Full Text] [PDF]

				Y. Ikegaya, S. Matsuura, S. Ueno, A. Baba, M. K. Yamada, N. Nishiyama, and N. Matsuki beta -Amyloid Enhances Glial Glutamate Uptake Activity and Attenuates Synaptic Efficacy J. Biol. Chem., August 23, 2002; 277(35): 32180 - 32186. [Abstract] [Full Text] [PDF]

				K. P M Currie and A. P Fox Cause for excite-M-ent in adrenal chromaffin cells J. Physiol., May 1, 2002; 540(3): 729 - 729. [Full Text] [PDF]

				K. K. Cook and D. A. Fadool Two Adaptor Proteins Differentially Modulate the Phosphorylation and Biophysics of Kv1.3 Ion Channel by Src Kinase J. Biol. Chem., April 5, 2002; 277(15): 13268 - 13280. [Abstract] [Full Text] [PDF]

				K. P M Currie and A. P Fox Cause for excite-M-ent in adrenal chromaffin cells J. Physiol., May 1, 2002; 540(3): 729 - 729. [Full Text] [PDF]

This Article Abstract Full Text (PDF) Submit an eLetter Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Web of Science (12) Citing Articles via Google Scholar Google Scholar Articles by Magoski, N. S. Articles by Kaczmarek, L. K. Search for Related Content PubMed PubMed Citation Articles by Magoski, N. S. Articles by Kaczmarek, L. K.

Home  |   Search  |   Archive  |   Subscribe  |   Contact  |   Help