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The Journal of Neuroscience, June 15, 2002, 22(12):4932-4941

Growth Cone Collapse Induced by Semaphorin 3A Requires 12/15-Lipoxygenase

Keith Mikule, Jesse C. Gatlin, Becky A. de la Houssaye, and Karl H. Pfenninger

Department of Cellular and Structural Biology, University of Colorado School of Medicine, Denver, Colorado 80262


    ABSTRACT
TOP
ABSTRACT
INTRODUCTION
MATERIALS AND METHODS
RESULTS
DISCUSSION
REFERENCES

Detection of a repellent factor, such as a semaphorin (Sema), causes localized collapse of the growth cone and directs the neurite away from the repellent. Growth cone collapse results from concomitant cytoskeletal rearrangements and detachment of adhesion sites from the extracellular matrix, via mostly unknown signaling mechanisms. In cultures of dorsal root ganglion neurons, we found that Sema3A treatment stimulates the synthesis of the eicosanoid, 12(S)-hydroxyeicosatetraenoic acid (HETE), whereas Sema3A-induced growth cone collapse is prevented when 12(S)-HETE synthesis is blocked with an inhibitor of 12/15-lipoxygenase (LO). Exogenously applied product of 12/15-LO, 12(S)-HETE, mimics Sema3A-induced collapse. As observed by interference reflection and confocal microscopy, 12(S)-HETE causes the loss of growth cone adhesion sites. The adhesion site effect seems partially independent of the actin cytoskeleton because growth cones treated with Sema3A and 12/15-LO inhibitor remain spread despite actin cytoskeleton loss. These studies demonstrate that 12/15-LO activity is a necessary step in Sema3A collapse signaling in growth cones and suggest a mechanism for its action.

Key words: neurite pathfinding; lipoxygenase; semaphorin; growth cone adhesion; growth cone collapse; eicosanoid signaling


    INTRODUCTION
TOP
ABSTRACT
INTRODUCTION
MATERIALS AND METHODS
RESULTS
DISCUSSION
REFERENCES

Accurate axonal targeting requires that growth cones navigate precisely along pathways defined by attractive and repulsive cues (Tessier-Lavigne and Goodman, 1996). Guidance cues are detected by receptor-rich growth cone filopodia that continuously survey their microenvironment (Lauffenburger and Horwitz, 1996). Filopodial contact with positive chemotactic factors stimulates growth cone translocation, which results from a cycle involving F-actin assembly driving lamellipodial extension, distal adhesion of the newly formed lamellipodia (to form an adhesion site), and proximal de-adhesion of the growth cone from the substrate (Richards et al., 1997). In contrast, filopodial contact with a repulsive factor, such as thrombin or a semaphorin (Sema), triggers de-adhesion and growth cone collapse, followed by retraction (Keynes and Cook, 1992; de la Houssaye et al., 1999; Fritsche et al., 1999; Fournier et al., 2000a).

Semas affect axonal guidance, cell migration, and neoplastic transformation (Tessier-Lavigne and Goodman, 1996; Soker et al., 1998; Behar et al., 1999). Sema3A is a class III secreted polypeptide that binds and activates a receptor complex consisting of neuropilin-1 (NP-1) and plexin A (Takahashi et al., 1999). When applied as a gradient, Sema3A causes growth cones to turn away from the source; incubation of cultured neurites with Sema3A triggers collapse of their growth cones (Fan and Raper, 1995). Sema3A-/- mice exhibit excessive growth of peripheral dorsal root ganglion (DRG) axons, reflecting the lack of normal Sema3A repulsion in vivo (Taniguchi et al., 1997). Several molecules involved in growth cone collapse have been identified. They include RhoA, Rac1, Cdc42, Rnd, and "collapse response mediator proteins" (CRMPs) and seem to be associated with the cytoskeletal effects induced by Sema3A (Goshima et al., 1995; Jin and Strittmatter, 1997; Zanata et al., 2002). Additionally, intracellular levels of cGMP have been shown to influence Sema3A-induced growth cone turning (Song et al., 1998). However, the signaling pathway leading to Sema3A-induced collapse is mostly unknown.

Amoeboid systems like growth cones, polymorphonuclear leukocytes, and platelets possess high levels of free arachidonic acid (AA) (Mazurov et al., 1983; Doukas et al., 1988; Negre-Aminou and Pfenninger, 1993). The free AA found in growth cones results from the action of at least two different cytoplasmic forms of phospholipase A2 (cPLA2) (Negre-Aminou and Pfenninger, 1993). We also demonstrated that isolated growth cones treated with the repellent thrombin greatly increase AA levels by activation of cPLA2. Although most AA is rapidly reincorporated into phospholipid, some of it is converted by leukocyte 12/15-lipoxygenase (LO) to 12(S)- and 15(S)-hydroperoxyeicosatetraenoic acid, which is subsequently reduced to 12(S)- and 15(S)-hydroxy-eicosatetraenoic acid (HETE) (Dailey and Imming, 1999; Yamamoto et al., 1999). Experiments on growth cones in culture show that 12/15-LO is necessary for thrombin-induced collapse, and 12(S)-HETE was shown to be sufficient for growth cone collapse in cortical neurons (de la Houssaye et al., 1999). The present studies on DRG neurons test the hypothesis that at least one of the Semas, Sema3A, also uses a signaling pathway involving 12/15-LO to trigger growth cone collapse and that this pathway affects growth cone adhesion.


    MATERIALS AND METHODS
TOP
ABSTRACT
INTRODUCTION
MATERIALS AND METHODS
RESULTS
DISCUSSION
REFERENCES

Materials. Experiments were performed with recombinant Sema3A used either as partially purified protein or as Sema3A-enriched culture supernatant of stably expressing 293 cells. Enrichment was achieved by concentrating the culture medium on a Centriplus membrane (Millipore Corporation, Bedford, MA; 50,000 MW cutoff). Partially purified Sema3A and Sema3A-expressing 293 cells were the generous gift of Dr. Yuling Luo (Exelixis Pharmaceuticals, Inc., South San Francisco, CA). Sema3A preparations were calibrated based on the degree of the collapse response and compared with that achieved with the partially purified factor (~1 nM). Antibody to NP-1 was a gift from Dr. Alex Kolodkin (The Johns Hopkins University School of Medicine). Cinnamyl-3,4-dihydroxy-alpha -cyanocinnamate (CDC), 12(S)-HETE, and indomethacin were purchased from Biomol. 5(R)-HETE was obtained from Calbiochem (LaJolla, CA). NGF was from Collaborative Biomedical Products. All other chemicals, unless stated otherwise, were from Sigma (St. Louis, MO) and of the highest quality obtainable.

Neuron culture. DRGs were dissected from embryonic day 15 Sprague Dawley rats and cultured on laminin-coated cover glass (Assistant Brand; Carolina Biological Supply Co., Burlington, NC) in B27 neurobasal medium (NB) supplemented with 10% FBS and 100 ng/ml NGF. The cultures were incubated at 37°C in 5% CO2 in air. Sprouting neurons were observed readily on day 1. Long neurites were present on day 2 or 3 and used for experiments.

12(S)-HETE immunobinding assay. DRG neurons were cultured as described above and used for experimentation on day 1.5. Cultures that received CDC did so 30 min before challenge with Sema3A. For 3 min before extraction, cultures were incubated at 37°C with Sema3A, CDC (10 µM), CDC (10 µM) followed by Sema3A, or left untreated. During extraction with detergent-containing buffer, 12(S)-HETE was assayed quantitatively by immunobinding kit (Assay Designs, Ann Arbor, MI) according to the instructions of the supplier. The assay uses a polyclonal antibody to 12(S)-HETE to bind, in a competitive manner, the 12(S)-HETE contained within a sample or an alkaline phosphatase-12(S)-HETE conjugate. This assay is capable of measuring ~120 pg/ml, is linear to 9500 pg/ml, is stereo-specific, and is minimally cross-reactive to other eicosanoids.

Growth cone collapse assay. DRG cultures were preincubated (at 37°C) with inhibitor or vehicle control (DMSO or ethanol) for 45-60 min before they were inverted onto media-filled Coverwell (Grace Bio-Labs) imaging chambers. The imaging chambers were then placed onto the heated stage (37°C) of a Zeiss Axiophot microscope and allowed to equilibrate for 15 min before repellent factor treatment. Images were captured at t = -5, 0, 1, 2.5, 5, 7.5, 10, and 15 min after the addition of repellent using a Kodak digital camera. Growth cone collapse was assessed quantitatively by recording the area of growth cone spread before and after treatment (using NIH image 1.62 software). The results were expressed as the percentage change in growth cone area. Student's t test was used to assess the statistical significance of experimental results.

Interference reflection microscopy. Coverslips with DRG cultures were mounted onto viewing chambers in medium, covered with mineral oil, and analyzed on a heated stage with an appropriately equipped Zeiss Axiovert 200M microscope. Images were analyzed by NIH image 1.62. The density slice function was used to isolate the darkest areas of a growth cone image. Total area of that region was measured. Student's t test was used to assess the statistical significance of experimental results.

Fluorescence imaging. After experimentation, cultures were fixed by slow infusion of 4% paraformaldehyde in 0.1 M phosphate buffer, pH 7.4, into the culture dishes over 10 min (Pfenninger and Maylie-Pfenninger, 1981). Thereafter, the fixative was removed by rinsing three times with PBS containing 1 mM glycine. Cultures were blocked with PBS/1% bovine serum albumin (BSA) and permeabilized by incubation in 0.02% Triton X-100 for 5 min. After three washes in PBS/BSA, cultures were ready for staining.

To stain for F-actin, cultures were incubated with Texas-Red-conjugated phalloidin for 30 min. Unbound phalloidin was washed away by two PBS rinses, after which the cover glasses were mounted in anti-FADE reagent (Molecular Probes, Eugene, OR). Paxillin and NP-1 were visualized by incubating the cultures with either anti-paxillin antibody (Transduction Laboratories, Lexington, KY) or anti-NP-1 antibody 1:100 in PBS/BSA at room temperature for 1 hr. Cultures were washed again and incubated for 45 min with either Texas-Red-conjugated goat anti-rabbit IgG for NP-1 (1:100; Molecular Probes) or Oregon-Green-conjugated goat anti-mouse IgG (1:100; Molecular Probes) and Texas-Red-conjugated phalloidin (5 U/ml; Molecular Probes) for dually labeled samples. After three washes, the coverslips were mounted onto slides in anti-FADE reagent.

Coverslips were examined with a Zeiss Axiophot fluorescence microscope equipped with epi-illumination. Confocal images were captured using an Olympus IX70 inverted microscope equipped with a Photometrics PXL camera (67 nm per image pixel with a 100× oil immersion objective) and a Silicon Graphics O2 computer with DeltaVision deconvolution software (Applied Precision).


    RESULTS
TOP
ABSTRACT
INTRODUCTION
MATERIALS AND METHODS
RESULTS
DISCUSSION
REFERENCES

Neuropilin-1 is distributed throughout the growth cone

Indirect immunofluorescence was performed on cultures of primary DRG neurons to determine the prevalence of NP-1-positive neurites in our preparations and to assess morphologically the distribution of NP-1 in their growth cones. Specific NP-1 fluorescence was observed in all neurons and neurites and thus confirmed that our DRG cultures are a valid system for studying quantitatively the growth cone responses to Sema3A (Fig. 1). Controls that omitted the primary antibody were free of fluorescence (data not shown).



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  		Figure 1.   Immunolocalization of NP-1 in NGF-responsive growth cones of DRG neurons. Left panel, Phase-contrast image shown for comparison. Right panel, Immunofluorescence image of bound anti-NP-1. Nonspecific fluorescence in controls processed without primary antibody was below the level of detection. Anti-NP-1 generates a diffuse, punctate pattern of immunoreactivity that is detected throughout the neurite and growth cone and extends into the finest filopodia. The asterisk indicates an area of high fluorescence intensity discussed in Results. Scale bar, 15 µm.

Detailed examination of the growth cones revealed punctate NP-1 staining. Labeling extended, at a moderate level, from the proximal domain into the thinnest regions of the growth cone periphery and into the finest filopodia. The staining in these distal regions appeared uniformly punctate. Robust labeling of neurite bundles indicated the presence of NP-1 in neurite shafts. Whether all NP-1 was on the cell surface, and whether its level was higher on neurites than on growth cones, however, was not evident because the optical superimposition of images generated by neurite fascicles contained much more plasma membrane than those of the growth cone periphery. In addition, cytoplasmic membrane elements were present. Areas of intense fluorescence (Fig. 1, asterisk) were observed occasionally in preterminal areas of fasciculated neurites. These may represent accumulations of NP-1-rich precursor membrane waiting to be inserted into the growth cone plasmalemma, they may be the result of NP-1 internalization, or they may serve some other, unknown function.

Eicosanoid generation in DRG neurons

We know that thrombin-treated cortical neurons and isolated growth cones generate 12(S)-HETE and that 12(S)-HETE is necessary for growth cone collapse in that system (de la Houssaye et al., 1999). To test our current hypotheses, we first examined whether Sema3A stimulated the synthesis of 12(S)-HETE. 12(S)-HETE levels were determined in sprouting DRG cultures using an immunobinding assay. Resting 12(S)-HETE levels were ~11 fmol/µg protein (Table 1). This value is based on total protein in the cultures, rather than just growth cone protein. Because cPLA2 and 12/15-LO activities are enriched in growth cones (Negre-Aminou and Pfenninger, 1993; de la Houssaye et al., 1999), the actual level of 12(S)-HETE in DRG growth cones is likely to be higher. In response to Sema3A treatment for 2 min, the level of 12(S)-HETE increased up to approximately ninefold (Table 1). The limited number of DRG cultures that could be prepared in a single session did not allow for the generation of a dose-response curve. However, in two separate experiments an increase in Sema3A concentration from ~0.5 to ~1 nM raised the level of 12(S)-HETE detected 4.9- and 2.7-fold (3.8-fold average). In parallel experiments, the same increase in Sema3A concentration caused a 4.1-fold enhancement in collapse (area decrease, see below). Thus, Sema3A-stimulated 12(S)-HETE synthesis was dose dependent and paralleled the collapse response, at least within a limited concentration range. The Sema3A-stimulated increase in 12(S)-HETE was reduced to below resting level by preincubating the cultures with CDC for 45 min before exposure to Sema3A. In the micromolar range, CDC is a selective inhibitor of LOs, including 12/15-LO (Cho et al., 1991). In cultures that were treated with CDC only, 12(S)-HETE was not detectable.


                              
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  		Table 1.   Sema3A stimulates 12(S)-HETE generation in sprouting DRG neurons

Is 12/15-LO necessary for Sema3A-induced collapse?

To answer this question we performed quantitative collapse assays on growth cones of rat DRG neurons in culture. Figure 2 shows in representative phase-contrast micrographs the effects of the experimental treatments on growth cone morphology at the beginning (t = 0) and end of a 7.5 min challenge with a subsaturating concentration (~0.5-1.0 nM) of Sema3A. Quantitation of growth cone collapse was accomplished by measuring total area of the same live growth cones, at the beginning and after 7.5 min of experimental treatment. This was done for 25 growth cones per experimental condition. Measurement of the same growth cones in this before/after paradigm had the advantage of determining actual change in area independent of the highly variable starting configuration of the growth cone. Quantitative results were expressed as the percentage change in growth cone area measured over the experimental time period and are shown in Figure 3.



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  		Figure 2.   Effects of Sema3A, of an LO inhibitor (CDC), of LO inhibitor plus Sema3A, and of LO inhibitor plus 12(S)-HETE on growth cone morphology. Phase-contrast micrographs show growth cones in cultures of rat DRG neurons that were either pretreated with vehicle alone or with CDC for 45 min before challenge with Sema3A or 12(S)-HETE. Images in the top row were taken at time 0; those in the bottom row were taken after 7.5 min of challenge with collapsing agent. Left to right, 10 µM CDC pretreatment, 10 µM CDC pretreatment followed by Sema3A (~0.5 nM), Sema3A (~0.5 nM), and 10 µM CDC pretreatment followed by 12(S)-HETE (100 nM). Arrows point to growth cones that are collapsing. Scale bar, 20 µm.



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  		Figure 3.   Quantitative analysis of collapse measured as percentage of change in growth cone area after 7.5 min of experimental treatment ± SEM. Twenty-five growth cones were measured for each condition over a minimum of three independent experiments. Left to right: 10 µM CDC pretreatment; Sema 3A (~0.5 nM); 10 µM CDC pretreatment followed by Sema3A (~0.5 nM); 12(S)-HETE (100 nM); 10 µM CDC pretreatment followed by 12(S)-HETE (100 nM); 10 µM indomethacin pretreatment followed by Sema3A (~0.5 nM); and 5(R)-HETE.

Within 3 min of Sema3A treatment, many growth cones lost their lamellipodial veils (data not shown). Treatment for 7.5 min (Fig. 2) caused the expected collapse, with a highly significant reduction of >30% of growth cone area (Fig. 3) (p < 10-4) and concomitant loss of filopodia and lamellipodia. Increasing the Sema3A concentration enhanced the collapse response to reach a saturating level at ~75% of area reduction (data not shown). This resulted often in neurites terminating in a "club"-shaped structure that sometimes possessed a few filopodia. In contrast to thrombin and lysophosphatidic acid-induced collapse (Jalink et al., 1993; de la Houssaye et al., 1999), little or no neurite retraction was observed for Sema3A in this time frame.

To determine whether signaling involved in Sema3A-induced growth cone collapse requires 12/15-LO activity, we preincubated DRG neurons with CDC, a potent and selective LO inhibitor. As a control we used indomethacin, a specific inhibitor of cyclooxygenases, thereby blocking the synthesis of prostaglandins and related eicosanoids, but not of HETEs. The growth cones of cultures pretreated with only CDC and observed over time (up to 2 hr) were fully spread out and veiled with extensive lamellipodia and filopodia (Fig. 2, CDC). By our current criteria they were indistinguishable from untreated cultures.

In cultures pretreated with CDC, Sema3A exposure for 7.5 min (Figs. 2, 3, CDC+SEMA3A) failed to induce growth cone collapse. These growth cones retained their lamellipodia and filopodia and, in some cases, continued to advance. Quantitatively growth cone areas remained indistinguishable (two-tailed t test) from those in CDC-only or control cultures. In contrast, if cultures were pretreated with indomethacin before challenge with Sema3A (Fig. 3, IND+Sema3A), growth cones collapsed, losing ~25% of their area, essentially as though they had not been pretreated.

Is the 12/15-LO product, 12(S)-HETE, sufficient to induce growth cone collapse?

To examine whether the products of 12/15-LO are sufficient for growth cone collapse, we applied exogenous 12(S)-HETE to cultures that had or had not been preincubated with CDC (Figs. 2, 3, CDC+12(S)-HETE, 12(S)-HETE). These experiments revealed that 12(S)-HETE caused collapse alone and even in the presence of CDC when 12/15-LO was inhibited. In both cases, 12(S)-HETE-treated growth cones gradually withdrew their lamellipodia until they lost both lamellipodia and filopodia. After 7.5 min of 12(S)-HETE treatment, growth cones exhibited a significant loss of ~20% of total area (Fig. 3) regardless of whether they had been pretreated with CDC (p < 0.06 for HETE alone; p < 0.04 for CDC + HETE). This degree of area reduction was more modest than that achieved by Sema3A but nevertheless significant. To rule out possible nonspecific effects that may be induced by the eicosanoid treatment of our cultures, we also tested a regio-isomer, 5(R)-HETE. Interestingly, the addition of 5(R)-HETE to cultures caused an increase (~10%), rather than a decrease, in growth cone area (Fig. 3), which indicated regio-isomer specificity of the HETE effects. These data demonstrate that 12/15-LO is necessary for Sema3A-induced growth cone collapse and that 12(S)-HETE, but not 5(R)-HETE, is sufficient to induce collapse.

Effects of Sema3A and HETE on adhesion sites

Growth cone collapse must occur as a result of at least two mechanisms: loss of F-actin from the growth cone periphery and disruption of growth cone adhesion sites (de la Houssaye et al., 1999; Zhou and Cohan, 2001). To test directly whether 12(S)-HETE caused alteration of adhesion sites, we used interference reflection microscopy (IRM), which allows the investigator to view, in live cells, changes in cellular adhesion sites. IRM images areas of close apposition of the plasma membrane to the glass coverslip (<10 nm) as very dark; areas of more open cellular apposition appear lighter than background (Izzard and Lochner, 1976).

Figure 4 shows representative IRM images taken of growth cones before and after 7.5 min of challenge. At the onset of challenge, growth cones exhibited a variable but primarily punctate and somewhat radial pattern of close (dark) adhesions with occasional bright (intermediate) adhesion areas (Fig. 4, left panels). After Sema3A or 12(S)-HETE challenge, the growth cone contact area was reduced greatly. Some close contact areas were changed to bright (Sema3A, arrowhead), indicating loosened adhesion. To quantify these changes, growth cone images were processed digitally by thresholding to isolate and measure the dark adhesion sites (Fig. 5A). Over the 7.5 min experimental time period, dark areas of control growth cones did not change significantly (-8 ± 6%; mean ± SEM). In contrast, Sema3A or 12(S)-HETE treatment of the cultures decreased the dark regions by ~45%, a value that is greater than the growth cone area decrease reported above (Fig. 3). This provides direct evidence that Sema3A and exogenous 12(S)-HETE rapidly cause at least partial dissociation of adhesion sites in growth cones. Quantitatively, i.e., for the population of growth cones analyzed, CDC pretreatment plus Sema3A challenge did not reduce close contact area. Of particular interest was the appearance of a prominent, almost continuous distal band of attachment. Instead of the primarily radial pattern of dark areas at 0 min, we observed an IRM-dark peripheral band adjacent to a more distal IRM-bright rim of close and intermediate attachment, respectively (Fig. 3, CDC+Sema3A, 7.5 min). Although IRM patterns of thin structures must be interpreted with caution, a marginal band of adhesion was evident.



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  		Figure 4.   Sema3A and 12(S)-HETE treatment of DRG growth cones causes a loss of adhesive area. IRM images were taken before (left) and after (right) 7.5 min of treatment with (top to bottom) Sema3A (~1 nM), Sema3A after 30 min CDC (10 µM), or 12(S)-HETE (100 nM). Arrows point at an area of conversion from predominantly dark (close contact) to bright (intermediate contact).



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  		Figure 5.   Quantitative analysis of the decrease in IRM-dark areas under various experimental conditions. A, Density slices of a growth cone before and after treatment (7.5 min) with Sema3A (~1 nM). These were derived from the top panels of Figure 4. B, Changes in the IRM-dark areas (close contacts) of growth cones treated with Sema3A (~1 nM) or 12(S)-HETE (100 nM) for 7.5 min, without or with CDC preincubation (30 min). control, No treatment. Data are averages ± SEM (n >=  5, except for CDC + Sema3A, where n = 2).

With a complementary approach we examined how both the actin cytoskeleton and adhesion sites were affected by our experimental treatments. We fixed cultures after challenge and then stained them with fluorescent labels for F-actin and the adhesion site protein, paxillin. In previous studies paxillin has proven to be an excellent marker of adhesion sites (Ross et al., 2000). Figures 6 and 7 show the confocal images of the attachment areas of the growth cones. CDC-treated (Fig. 6, CDC) growth cones exhibited broadly spread lamellipodia with F-actin-positive radial ridges that originated near the neurite shaft and extended to the distal edge of the growth cone. The area of greatest label intensity was near or at the distal edge. Paxillin staining in these cultures appeared as a partially overlapping, punctate pattern. It was particularly dense in the neurite and central region of the growth cone but extended all the way into the periphery. The function of the high paxillin level in the neurite is unknown. Relative to the other peripheral areas of the growth cone, the paxillin puncta seemed to be slightly more concentrated along the distal edge and the radial ridges highlighted by F-actin. This observation is consistent with a requirement for distal adhesion sites in the spread growth cone.



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  		Figure 6.   Effects of CDC and Sema3A on growth cone F-actin and paxillin as shown by confocal microscopy. Images are optical sections through the attachment area. DRG growth cones were pretreated with 10 µM CDC for 45 min before challenge (7.5 min) with control medium or Sema3A (~0.5 nM). Collapse effects paralleled those reported in Figure 3. F-actin is shown in red, paxillin is shown in green, and areas of overlap appear yellow. From top to bottom, panels show merged images, F-actin only, and paxillin only. Scale bar, 15 µm.



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  		Figure 7.   Effects of Sema3A and 12(S)-HETE on growth cone F-actin and paxillin as shown by confocal microscopy. Images are optical sections through the attachment area. DRG growth cones were challenged with Sema3A (~0.5 nM) or 12(S)-HETE (100 nM). Collapse effects paralleled those reported in Figure 3. To illustrate the relative changes in actin and paxillin distribution, however, the growth cone example for 12(S)-HETE (left) is one of incomplete collapse. F-actin is shown in red, paxillin is shown in green, and areas of overlap appear yellow. From top to bottom, panels show merged images, F-actin only, and paxillin only. Scale bar (shown in Fig. 6), 15 µm.

After 7.5 min of Sema3A treatment (Fig. 7, Sema3A), growth cones had lost their lamellipodia and all but a few of their filopodia, with the actin cytoskeleton being collapsed to a proximal, "clumped" pattern, where it colocalized with paxillin. This is consistent with what others have observed (Fournier et al., 2000b). Interestingly, we observed a very similar response in thrombin-treated cortical neurons (de la Houssaye et al., 1999), which supports the idea that proximal "clumping" of actin may represent a universal feature of growth cone collapse.

We also examined cultures that were pretreated with CDC before Sema3A challenge. In most cases (Fig. 6, CDC+Sema3A), the growth cones remained spread out and retained their lamellipodia despite having lost their radial actin ridges. Fluorescent intensity for both paxillin and F-actin was strongest along the distal edge of the growth cone, where they colocalized (yellow). This yellow band seemed to correspond to the peripheral band of attachments observed by IRM in the same type of experiment (Fig. 4). This led us to suspect that the adhesion sites remained intact, especially along the distal edge, notwithstanding the compromised structural integrity associated with loss of distal F-actin. Some degree of proximal F-actin clumping also was evident in these growth cones.

Growth cones of DRG neurons treated with 12(S)-HETE lost a significant fraction of their spread area and close contacts (Figs. 2, 3, 4). However, in growth cones that were not yet fully collapsed (Fig. 7, 12(S)-HETE), we observed that a significant amount of F-actin remained distributed in the growth cone periphery, chiefly in filopodia, with some actin ridges still evident (see corresponding single channel image). Little or no actin clumping was observed proximally in these cases. Paxillin was distributed in a somewhat more condensed punctate pattern, extensively colocalized with F-actin. The paxillin-enhanced distal edge of the growth cone, characteristic of CDC + Sema3A experiments, was not evident.


    DISCUSSION
TOP
ABSTRACT
INTRODUCTION
MATERIALS AND METHODS
RESULTS
DISCUSSION
REFERENCES

Sema3A triggers growth cone collapse in neurons expressing NP-1/plexin A receptors (Goshima et al., 1999; Reza et al., 1999; Takahashi et al., 1999), but the identification of the signaling steps leading to collapse has remained elusive. We report that Sema3A increases 12(S)-HETE levels in DRG neurons and that this eicosanoid is necessary for growth cone collapse. We also show that 12(S)-HETE is sufficient to cause collapse and that it does so by reducing growth cone adhesion.

Role of eicosanoid in Sema-induced collapse

Our cultured DRG neurons exhibited a uniform population of NP-1 receptor-positive growth cones. This enabled us to investigate the role of 12/15-LO in Sema3A signaling. Cellular eicosanoid generation depends on the supply of arachidonic acid, a PLA2 cleavage product. Indeed, the collapsing factor, thrombin, greatly stimulates PLA2 activity in isolated growth cones (de la Houssaye et al., 1999). However, PLA2 is such a widespread biological activity that its changes (e.g., triggered by Sema3A) in a subset of cellular elements (e.g., growth cones in DRG cultures) are difficult to detect against background levels. The next step in 12(S)-HETE synthesis, catalyzed by 12/15-LO, appears to be more specific. The recent introduction of a highly sensitive immunobinding assay for 12(S)-HETE has made it possible to measure Sema3A-induced changes in the level of this eicosanoid.

When exposed to Sema3A, DRG cultures increased the level of 12(S)-HETE several-fold. This increase was comparable to that observed for thrombin-treated, isolated growth cones (de la Houssaye et al., 1999). In these earlier growth cone studies, we measured rates of eicosanoid synthesis from exogenous radiolabeled substrate. Because those data are not directly comparable to the actual eicosanoid levels in DRG cultures shown here, we analyzed isolated growth cones with both assays in parallel, using thrombin as the repellent factor. Our results (data not shown) suggest that the 12(S)-HETE accumulation expected from the measured, thrombin-stimulated increase in eicosanoid synthesis [radiolabel assay; see de la Houssaye et al. (1999)] is comparable to the actual increase in 12(S)-HETE as determined by immunobinding. Also of importance, at least within a limited range of Sema3A concentrations, growth cone collapse and 12(S)-HETE generation increase roughly in parallel, suggesting that 12/15-LO may play a role in Sema3A-induced growth cone collapse, as in thrombin signaling.

To test this hypothesis we inhibited 12/15-LO activity by pretreatment of the cultures with CDC, a selective blocker of LOs (Cho et al., 1991). Biochemical measurements showed that CDC decreased even Sema3A-stimulated 12(S)-HETE levels to below control. In these conditions, Sema3A failed to cause growth cone collapse. In contrast, the cyclooxygenase inhibitor, indomethacin, did not interfere with collapse triggered by this repellent. These results indicated that 12/15-LO is necessary for Sema3A-induced collapse. To assess whether 12(S)-HETE is sufficient to cause collapse, we applied exogenous 12(S)-HETE to DRG growth cones, with or without previous CDC incubation. As in the case of cortical neurons (de la Houssaye et al., 1999), growth cones of DRG neurons collapsed in response to 12(S)-HETE, regardless of the presence of CDC. The result indicates that this eicosanoid can reverse the CDC inhibition of collapse and that it is sufficient to cause collapse. Interestingly a regio-isomer, 5(R)-HETE, increased rather than decreased growth cone spread by a small, but significant, amount. Therefore, the 12(S)-HETE-induced collapse is a specific effect of this isomer.

Eicosanoids and growth cone adhesion

Our earlier experiments with isolated growth cones demonstrated that thrombin or thrombin receptor-activating peptide causes growth cone detachment from the substratum independent of the actin cytoskeleton. Because experiments involving isolated growth cones are not feasible for DRG neurons, we examined their attachment to the substratum by IRM. Within minutes, Sema3A and exogenous 12(S)-HETE were seen to reduce areas of both intermediate and close contacts. Close-contact areas were shown to change significantly, and this reduction in contact was greater than the decrease in growth cone area observed under the same conditions. However, CDC inhibited the Sema3A-induced reduction in contact area. These observations indicate that 12(S)-HETE diminishes growth cone adhesion.

To study changes in growth cone adhesion in further detail, we analyzed growth cone collapse by confocal microscopy, using paxillin antibody and phalloidin to reveal adhesion sites and F-actin, respectively. The observed high level of paxillin in the neurite shaft is surprising and difficult to explain at the present. The images of control (or CDC pretreated) and of Sema3A-collapsed growth cones were consistent with earlier reports (Fournier et al., 2000b). Most striking was the result of the CDC + Sema3A experiment: growth cones remained spread out and exhibited a distal, continuously labeled rim of paxillin as if the adhesion sites had consolidated into an adherent margin. The usually radial F-actin pattern had disappeared, but a distal rim of actin, colocalized with paxillin, persisted. Corresponding IRM images did indeed show such a distal rim of attachment. In other words, growth cones remained spread and attached by a peripheral band of paxillin- and F-actin-containing adhesions despite Sema3A-induced F-actin loss more proximally. This result is consistent with what we observed in previous studies in which CDC + thrombin-treated growth cones of cortical neurons remained spread, although the majority of F-actin was lost (de la Houssaye et al., 1999). Therefore, inhibition of 12/15-LO does not appear to block F-actin loss within the time frame of these observations.

In contrast to the previous experiment, the paxillin- and F-actin-containing rim seen after CDC + Sema3A was not evident in growth cones treated with 12(S)-HETE or with CDC + 12(S)-HETE (data not shown). The growth cones of 12(S)-HETE-treated neurons collapsed, although F-actin retained a rudimentary radial pattern. This would be expected initially, if an otherwise intact growth cone were to lose first its attachments to the growth substratum. These findings are consistent with the 12(S)-HETE-induced loss of paxillin from adhesion sites that we observed in pseudopods of carcinoma cells (Ross et al., 2000).

Structurally, the integrity of adhesion sites and that of the F-actin cytoskeleton are known to be interdependent (Critchley et al., 1999; Small et al., 1999). Our data suggest, however, that growth cone collapse depends to a significant degree on the disassembly and detachment of adhesion sites, via a signaling pathway that may be partially distinct from that regulating the actin cytoskeleton. Support for this interpretation stems from the preferential inhibition of adhesion site disassembly by CDC, as shown here, and from the fact that thrombin-induced growth cone detachment does not require a functional actin cytoskeleton (de la Houssaye et al., 1999). Our observations suggest, furthermore, that the 12/15-LO product, 12(S)-HETE, affects primarily the adhesion sites.

The search for molecules that mediate Sema-induced growth cone collapse has identified a number of potential signaling elements. Members of the Rho family of GTPases, especially Rac1, have been implicated in collapse (Jin and Strittmatter, 1997; Zanata et al., 2002). Rac1 seems to control the changes of the actin cytoskeleton associated with collapse (Kuhn et al., 1999). However, Rho family GTPases also are necessary for growth cone spreading (Mackay et al., 1995), and Rho may be more important for neurite retraction than collapse (Arimura et al., 2000). Also, inhibition of Rho blocks lysophosphatidic acid-mediated growth cone collapse in neuronal cell lines (Jalink et al., 1994) but causes growth cone collapse in sensory neurons (Jin and Strittmatter, 1997). There is no doubt that Rho family GTPases are involved in the cytoskeletal reorganizations accompanying growth cone spreading and collapse, but it remains unclear whether they are steps in the repellent receptor-activated signaling cascade. The CRMPs (Goshima et al., 1995) have been implicated in collapse but today seem more likely to regulate microtubule dynamics and axon formation (Gu and Ihara, 2000; Inagaki et al., 2001). Therefore, a consistent picture of repellent signaling has failed to emerge so far.

How 12(S)-HETE regulates the disassembly of adhesion sites and collapse is unknown. Is there cross talk between 12(S)-HETE generation and the rearrangement of the actin cytoskeleton? Interestingly, 12(S)-HETE has been implicated indirectly in Rac1 signaling (Wen et al., 2000). This could explain our observation that 12(S)-HETE treatment of cultured DRG growth cones causes partial F-actin loss, in addition to adhesion site disassembly. However, our own preliminary data suggest that 12(S)-HETE activates a PKC isoform, which in turn leads to the phosphorylation of the adhesion site protein myristoylated alanine-rich C-kinase substrate (our unpublished observations), a protein involved in cell spreading (Rosen et al., 1990; Myat et al., 1997; Laux et al., 2000).

Conclusions

Our data demonstrate that Sema3A treatment of DRG growth cones stimulates the synthesis of 12(S)-HETE and that its generation is necessary for growth cone collapse and detachment. 12(S)-HETE mimics, and therefore is sufficient to cause, collapse when added to DRG growth cones exogenously. Furthermore, our evidence suggests that increases in 12(S)-HETE levels cause a reduction of growth cone adhesion. These observations are consistent with our previous finding that thrombin-treated cortical neurons require 12/15-LO activity for collapse and that collapse involves regulated detachment. Therefore, the generation of 12(S)-HETE and the regulation of adhesion sites may be general phenomena of repellent signaling.


    FOOTNOTES

Received Aug. 3, 2001; revised March 15, 2002; accepted March 22, 2002.

This work was supported by National Institutes of Health Grants NS24672 and NS41029 to K.H.P. We thank Dr. W. J. Betz for access to his confocal microscope and Steve Fadul for his help with this technology.

Correspondence should be addressed to Dr. Karl H. Pfenninger, Department of Cellular and Structural Biology, University of Colorado School of Medicine, B111 4200 East 9th Avenue, Denver, CO 80262. E-mail: Karl.Pfenninger{at}uchsc.edu.


    REFERENCES
TOP
ABSTRACT
INTRODUCTION
MATERIALS AND METHODS
RESULTS
DISCUSSION
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