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The Journal of Neuroscience, July 1, 2002, 22(13):5328-5333

Identification of a  Subunit TM2 Residue Mediating Proton Modulation of GABA Type A Receptors

Megan E. Wilkins, Alastair M. Hosie, and Trevor G. Smart

School of Pharmacy, Department of Pharmacology, University of London, London, WC1N 1AX, United Kingdom

	ABSTRACT

TOP ABSTRACT INTRODUCTION MATERIALS AND METHODS RESULTS DISCUSSION REFERENCES

GABA type A (GABA_A) receptors are functionally regulated by external protons in a manner dependent on the receptor subunit composition. Although H⁺ can regulate the open probability of single GABA ion channels, exactly what residues and receptor subunits are responsible for proton-induced modulation remain unknown. This study resolves this issue by using recombinant 1i subunit GABA_A receptors expressed in human embryonic kidney cells. The potentiating effect of low external pH on GABA responses exhibited p_Ka in accord with the involvement of histidine and/or cysteine residues. The exposure of GABA_A receptors to the histidine-modifying reagent DEPC ablated regulation by H⁺, implicating the involvement of histidine residues rather than cysteines in proton regulation. Site-specific substitution of all conserved external histidines to alanine on the  subunits revealed that H267 alone, in the TM2 domain, is important for H⁺ regulation. These results are interpreted as a direct protonation of H267 on 1i receptors rather than an involvement in signal transduction. The opposing functional effects induced by Zn²⁺ and H⁺ at this single histidine residue most likely reflect differences in charge delocalization on the imidazole rings in the mouth of the GABA_A receptor ion channel. Additional substitutions of H267 in  subunits with other residues possessing charged side chains (glutamate and lysine) reveal that this area of the ion channel can profoundly influence the functional properties of GABA_A receptors.

Key words: GABA_A receptor; pH modulation; subunit; histidine, H⁺; ion channel

	INTRODUCTION

TOP ABSTRACT INTRODUCTION MATERIALS AND METHODS RESULTS DISCUSSION REFERENCES

GABA type A (GABA_A) receptors are the major mediators of inhibitory neurotransmission in the brain. Fast inhibition occurs via the synaptic GABA_A receptors (Smart, 1998), whereas extrasynaptic isoforms have been demonstrated to underlay continuous tonic inhibition (Otis et al., 1991). Those receptors expressed at inhibitory synapses will be regulated by endogenous processes, including trafficking, endocytosis, phosphorylation, redox reagents, and ions normally present in vivo (e.g., Zn²⁺ and H⁺) (Kaila, 1994; Sieghart, 1995; Rabow et al., 1996; Moss and Smart, 2001). Variations in the level of endogenous ions in the CNS represent a rapid and effective method for regulating receptor function (Kaila, 1994; Smart et al., 1994). Previous studies have indicated that protons can differentially affect neuronal GABA_A receptors, resulting in potentiation, inhibition, or no effect on GABA-activated responses (Kaila, 1994; Robello et al., 1994; Pasternack et al., 1996; Zhai et al., 1998; Krishek and Smart, 2001). This variability to pH has been ascribed to differences in the neuronal receptor subunit composition that can be reproduced to some extent using recombinant GABA_A receptors (Krishek et al., 1996).

GABA_A receptors are presumed to be pentameric proteins (Nayeem et al., 1994) composed of combinations of the following subunits: (1-6), (1-3), (1-3), , , , and  (Rabow et al., 1996; Mehta and Ticku, 1999). Notably, receptors composed of 11, 11, and 112 subunits were differentially sensitive to external pH, whereas the 1i2 subunit combinations (where i = 1,2) were primarily unaffected (Krishek et al., 1996). Subsequent single-channel studies in neurons indicated that raised H⁺ concentrations caused inhibition of GABA-activated responses by reducing the single-channel open probability with no effect on channel conductance (Huang and Dillon, 1999; Krishek and Smart, 2001). Furthermore, for cerebellar granule neurons, developmental changes in GABA_A receptor 1 and 6 subunit expression (Laurie et al., 1992) occurred concurrently with changes in GABA_A receptor sensitivity to external pH, suggesting that protons may be a useful probe for detecting changes in receptor subunit composition (Krishek and Smart, 2001).

Modulating GABA_A receptor function with external pH may be important during CNS trauma and neurological pathologies by causing changes in neuronal excitability (Xiong and Stringer, 2000). However, the underlying amino acid residues on GABA_A receptors that are responsible for the proton effects remain unidentified. The present study investigates this question using heterologous expression of GABA_A receptors and resolves that a histidine residue at position 267 in the ion channel domain of the  subunit is not only completely responsible for proton regulation of 1i receptor function, but also that after substitution, this location in the ion channel can profoundly influence the functional properties of the GABA_A receptor. To date, this histidine residue plays a unique role in the function of the GABA_A receptor's forming a potential ion binding site and significant transduction pathway. Some of these results have been reported previously in abstract form (Wilkins et al., 2001).

	MATERIALS AND METHODS

TOP ABSTRACT INTRODUCTION MATERIALS AND METHODS RESULTS DISCUSSION REFERENCES

cDNA constructs and site-specific mutagenesis. The murine GABA_A receptor 1, 1, and 2 subunit cDNAs were cloned into the vector pRK5. Site-specific mutagenesis was achieved by using oligonucleotides in conjunction with a primer-directed PCR method (Quickchange; Stratagene, La Jolla, CA), and purified DNAs were prepared using the Plasmid Maxi Kit (Qiagen, Crawley, UK). The entire coding region of all mutants was sequenced using the BigDye ready reaction mix (PerkinElmer Life Sciences, Emeryville, CA/Applied Biosystems, Foster City, CA) and an ABI 310 automated DNA sequencer (Applied Biosystems).

Cell culture and electroporation. Human embryonic kidney (HEK) cells were cultured as described previously (Wooltorton et al., 1997b). The cells were transfected by electroporation (Gene Electropulser II, Hemel Hempstead, UK) using the following parameters: 0.4 kV, 125 µF capacitance, and infinite resistance in the presence of 12 µg of cDNA for the GABA_A receptor subunits, present in equal ratios, and 3 µg of green fluorescent protein. Electroporated cells were plated onto poly-L-lysine-coated glass coverslips, which were used for electrophysiological recording 18-72 hr after transfection.

Patch-clamp electrophysiology. Membrane currents were recorded using a whole-cell patch-clamp technique from single HEK cells with an Axopatch 1-C amplifier (Axon Instruments, Foster City, CA). Patch pipettes (resistance, 3-5 M) were filled with a solution containing (in mM): 120 KCl, 1 MgCl₂, 11 EGTA, 30 KOH, 10 HEPES, 1 CaCl₂, 2 adenosine triphosphate, and 12 creatine phosphate, pH 7.11. The cells were continuously perfused with Krebs' solution containing (in mM): 140 NaCl, 4.7 KCl, 1.2 MgCl₂, 2.52 CaCl₂, 11 glucose, and either 5 HEPES or 5 MES (see pH buffers). The Krebs' pH was finally adjusted to pH 5.4-8.4 with 1 or 5 N NaOH. Membrane currents were filtered at 5 kHz (3 dB, sixth pole Bessel, 36 dB/octave) and analyzed with Clampex 8 (Axon Instruments). Any change of >10% in the membrane conductance/series resistance resulted in the cessation of recording. Drugs and solutions were rapidly applied to the cells using a modified Y-tube positioned ~300 µm from the recorded cell. The response rise times were within 20-30 msec (Wooltorton et al., 1997b). All drugs were dissolved in external Krebs' solution at the appropriate pH. DEPC was added directly to the Krebs' solution, immediately before use, to yield final concentrations of 1 mM.

pH buffers. To ensure accurate control of the Krebs' pH over the range 5.4-8.4, two different pH buffers were used. For pH excursions between 6.8 and 8.4, HEPES (p_Ka 7.5) was added to the Krebs' solution, and for the pH range 5.4-7, MES was used (p_Ka 6.1), both at 5 mM final concentrations.

Analysis of whole-cell current data. Peak amplitude membrane currents activated by GABA (I) were determined at 50 mV holding potential. GABA equilibrium concentration-response relationships were constructed by measuring the peak GABA currents, which were normalized to the response induced by 10 µM GABA in control Krebs' solution at pH 7.4 (I₁₀) and subsequently fitted with the Hill equation:

(1)

where EC₅₀ represents the concentration of GABA ([A]) inducing 50% of the maximal current evoked by a saturating concentration of GABA and n is the Hill coefficient.

When the maxima of the curves demonstrated a clear depression with incrementing GABA concentration, the data were fitted with the following equation, accounting for the reduced maximum response by assuming that the ligand binds to two distinct sites, one producing potentiation and the other producing inhibition (or desensitization) of the GABA-activated current:

(2)

where I, I₁₀, EC₅₀, and n are as defined, IC₅₀ is the GABA concentration producing a 50% inhibition of the current, and m represents the corresponding Hill coefficient. To ensure a fit to the inhibitory component, occasionally the IC₅₀ was constrained where limited numbers of data points were available.

The GABA concentration-response curve data were analyzed using ANOVA with a Bonferroni post hoc test. Significance was determined at the p < 0.05 level.

The Zn²⁺ inhibition concentration relationships were fitted with the following equation:

(3)

where I_N^' and I_N represent the normalized GABA-induced current in the presence and absence of Zn²⁺ at concentration B, respectively, and IC₅₀ defines the concentration of Zn²⁺ producing 50% inhibition of the GABA-induced current.

	RESULTS

TOP ABSTRACT INTRODUCTION MATERIALS AND METHODS RESULTS DISCUSSION REFERENCES

External pH and recombinant 1i GABA_A receptors

To assess whether the identity of the i subunit was important for the pH-induced regulation of GABA_A receptor function, recombinant GABA_A receptors composed of 11 and 12 were expressed in HEK cells and used to construct full GABA concentration-response curves at normal physiological pH 7.4 and after a pH excursion to 5.4 (Fig. 1). For either construct, increasing the total H⁺ concentration by 100-fold resulted in potentiated responses to GABA and an increased maxima for the concentration-response curves (p < 0.05 for the range of 5 µM to 1 mM GABA). The GABA EC₅₀s for 11 (pH 7.4, 5.0 ± 0.7 µM; pH 5.4, 3.3 ± 0.6 µM; n = 3-5) and 12 (pH 7.4, 3.2 ± 0.4 µM; pH 5.4, 4.9 ± 0.7 µM; n = 3-5) displayed no significant change, suggesting that GABA potency was primarily unaffected by the change in external pH (Fig. 1A,B). A direct comparison between the concentration-response curves for the two GABA_A receptor heteromers indicated that H⁺ induced a slightly larger potentiation of GABA-activated responses on 11 receptors compared with 12 receptors for 10 µM GABA-activated responses (potentiated by 197.6 ± 37.5 and 120.1 ± 15.5%, respectively; n = 5-7). An additional distinction between 11 and 12 GABA_A receptors was that at high GABA concentrations (>100 µM) in pH 5.4, the peak of the GABA concentration-response curve was depressed only for the 11 receptors, possibly indicative of inhibition or increased desensitization. Overall, these data suggested that the identity of the  subunit in 1i constructs, although partially influential, did not have a major effect on the extent and type of modulation by H⁺.

View larger version (22K): [in this window] [in a new window]   Figure 1.   External pH modulates the GABA concentration-response relationships. For 11 (A) and 12 (B) GABAA receptors, peak amplitude responses were measured at pH 7.4 () and pH 5.4 () and were normalized to the responses induced by 10 µM GABA at pH 7.4 (= 1). In this and comparable figures, the curves were generated according to Equations 1 and/or 2, presented in Materials and Methods. All points in this and comparable figures represent the mean ± SEM from n = 3-5 cells. The insets illustrate sample GABA-activated currents induced by 10 µM GABA applied for the duration of the solid line at the indicated external pH. Calibration: 200 pA, 2 sec.

Identifying the residues underlying H⁺ modulation of 1i GABA_A receptors

To establish the residue(s) that is important for the H⁺ modulation of 1i GABA_A receptors, we titrated the H⁺-induced potentiation for 11 and 12 GABA_A receptors against the external Krebs' pH; this yielded p_Kas of 6.73 ± 0.09 and 6.91 ± 0.13, respectively (n = 5). We therefore deduced that histidines (p_Ka 6) are the most likely candidates to be involved in proton modulation of GABA_A receptors. Although cysteine residues also have a comparable p_Ka (~8) they were considered unlikely to be involved, considering that the only two external cysteine residues, located within the N terminal, are postulated to form a disulfide bridge (Barnard et al., 1987; Pan et al., 1995, 2000; Amato et al., 1999), precluding their involvement with H⁺-induced modulation.

To investigate a potential role for histidine residues in H⁺ regulation of GABA_A receptor function, we used DEPC, a reagent that irreversibly converts neutral imidazole groups into N-carbethoxyhistidyl derivatives (Miles, 1977). HEK cells expressing 1i subunit constructs were exposed to 1 mM DEPC at pH 7.4. A brief application (4 min) of DEPC to 1i receptors irreversibly abolished the potentiation of GABA-activated responses observed at pH 5.4 in the absence of DEPC. Inspection of GABA concentration-response curves revealed that after DEPC treatment, lowering the external pH to 5.4 induced a small lateral shift in the GABA concentration-response relationship, causing a reduction in the potency of GABA (EC₅₀s for 11: pH 7.4, 5.1 ± 1.3 µM; pH 5.4, 18.8 ± 4.6 µM; p < 0.05) (Fig. 2). These data were in accord with one or more histidines underlying the H⁺-induced potentiation of GABA-activated responses, and this effect appeared to be masking a weak inhibitory action of H⁺ that was clearly manifest only when the histidines had been covalently modified.

View larger version (19K): [in this window] [in a new window]   Figure 2.   Modification of histidine residues by DEPC prevents proton-induced modulation of 11 GABAA receptors. Normalized GABA concentration-response relationships obtained at pH 7.4 () and pH 5.4 () after exposure to DEPC (1 mM) are shown. For comparison, the GABA concentration-response curves at pH 7.4 (dotted line) and pH 5.4 (dashed line) were taken from Figure 1A. Data are from n = 3-5 cells.

Site-specific mutagenesis of external histidines on 1i receptors

Considering the ablation of H⁺ modulation by DEPC, the exact molecular determinants of this potentiation were sought using site-specific mutagenesis. The 1i heteromers possess numerous external histidine residues, eight on the 1 subunit and four on the i subunits. External residues were selected because internal pH changes have little effect on GABA_A receptor function (Krishek et al., 1996). The  subunit was examined primarily because of the pivotal role this subunit family plays in the expression and function of GABA_A receptors (Connolly et al., 1996). The number of histidines considered to be potentially involved in the H⁺ modulation on the  subunit was reduced to three by including only those that are conserved between all  subunits, because H⁺ modulation of GABA_A receptor function is not critically dependent on the identity of the  subunit. The three candidate histidines on the 2 subunit were H107 and H119, located in the N terminal, and H267, located in TM2, the ion channel domain. Each histidine in the 2 subunit was sequentially substituted, initially to alanine, and coexpressed with wild-type 1 subunits in HEK cells.

Neither the mutant 12^H107A nor 12^H119A GABA_A receptors differed in their response to changing the external pH to 5.4, which still potentiated responses to GABA to levels similar to those observed with the wild-type 12 receptors (EC₅₀s: 12^H107A: pH 7.4, 4.1 ± 0.6 µM; pH 5.4, 4.8 ± 0.9 µM; 12^H119A: pH 7.4, 3.0 ± 0.4 µM; pH 5.4, 4.9 ± 0.5 µM; p > 0.05), compared with 12 wild type for both mutants (Fig. 3A,B). However, substituting H267 with alanine, previously identified to play a major role in Zn²⁺ inhibition of GABA-activated responses (Wooltorton et al., 1997a; Horenstein and Akabas, 1998) on 1i receptors, ablated the potentiating effect of H⁺ (Fig. 4). Although the GABA concentration-response curve for the mutant 11^H267A did not exhibit an enhanced maximum response at pH 5.4, it was evident for both 11^H267A and 12^H267A receptors that low pH caused a small reduction in GABA potency (EC₅₀s for 12^H267A: pH 7.4, 3.2 ± 0.4 µM; pH 5.4, 7.1 ± 1.9 µM; p > 0.05) (Fig. 4). This weak inhibitory effect of H⁺ was manifest between GABA concentrations of 1 and 30 µM and appeared analogous to the effect of DEPC on the GABA concentration-response curve for the wild-type 11 (Fig. 2) or 12 receptors.

View larger version (17K): [in this window] [in a new window]   Figure 3.   Mutating histidines 107 or 119 in the 2 subunit do not alter proton-induced modulation of 12 GABAA receptors. Normalized GABA concentration-response relationships for 12H107A (A) and 12H119A (B) at pH 7.4 () and pH 5.4 (). The comparative concentration-response curves for the wild-type 12 receptor at pH 7.4 (dotted line) and pH 5.4 (dashed line) were taken from Figure 1B. Data are from n = 3-5 cells.

View larger version (23K): [in this window] [in a new window]   Figure 4.   Mutation of the TM2 histidine 267 in the 2 subunit ablates proton sensitivity of 12 GABAA receptors. A normalized GABA concentration-response relationship for 12H267A GABAA receptors at pH 7.4 () and pH 5.4 () is shown, including the comparison with the curve fits for 12 receptors at pH 7.4 (dotted line) and pH 5.4 (dashed line). The inset demonstrates typical responses to 10 µM GABA recorded at pH 7.4 and pH 5.4 for 12H267A receptors. Calibration: 200 pA, 2 sec.

The lack of any potentiating effect of H⁺ on the GABA_A receptor incorporating the mutant H267A suggested that this histidine residue may form a key protonation site for H⁺ ions and is likely to be the only histidine involved. This latter point was reassessed by exposing 11^H267A GABA_A receptors to 1 mM DEPC. This modifying agent did not further affect the GABA concentration-response curves, nor did it affect the weak inhibitory effect revealed once the H⁺-induced potentiating effect had been abolished (data not shown).

Charged mutations at H267 in the  subunit

To further investigate whether H⁺ could be binding to H267, the residue that also has a pivotal role in Zn²⁺ inhibition, two additional substitutions were made in the 2 subunit for subsequent coexpression with wild-type 1 subunits. These substitutions introduced amino acids with charged side chains: glutamate (E, negative) and lysine (K, positive) at position H267. Glutamate was selected because it has a projected p_Ka for the side chain carboxyl group of 4 and probably less in the GABA_A receptor protein and would not be protonated at pH 7.4 (100% anionic) and only minimally at pH 5.4 (96.2% anionic). Thus, we predict that H⁺ will be ineffective at potentiating GABA-activated responses if they do indeed bind to H267. However, for Zn²⁺ inhibition, the ability of the glutamate carboxyl group to act as an electron donor suggests that it will be capable of binding Zn²⁺, a role that it fulfills in Zn²⁺-containing metalloenzymes (Vallee and Auld, 1990). Thus, if Zn²⁺ binds to H267, we would expect the retention of some inhibitory activity at the mutant 12^H267E receptor but possibly at a reduced level compared with the wild-type receptor containing 2^H267.

Exposure of 12^H267E receptors to low external pH 5.4 abolished the effect of H⁺ on GABA-activated responses, as predicted if this residue forms a site for protonation (EC₅₀: pH 7.4, 3.4 ± 0.5 µM; pH 5.4, 4.8 ± 1 µM; p > 0.05 compared with 12 wild type) (Fig. 5A). However, the level of Zn²⁺ inhibition over the concentration range of 1 µM to 10 mM for the 2^H267E substitution was less than expected (Fig. 6). The mutation disrupted antagonism, but the Zn²⁺ IC₅₀ was 30.6 ± 7.3 µM, almost identical to that determined previously for GABA_A receptors incorporating the mutation i^H267A (Wooltorton et al., 1997a) but greater than that for the 12 wild type (IC₅₀: 0.65 ± 0.03 µM; n = 4) (Fig. 6).

View larger version (20K): [in this window] [in a new window]   Figure 5.   Effect of charged mutations at position H267 in the  subunit on 12 GABAA receptor sensitivity to protons. Normalized GABA concentration-response relationships for 12H267E (A) and 12H267K (B) GABAA receptors at pH 7.4 () and pH 5.4 () are shown, together with curve fits for the 12 wild-type receptor at pH 7.4 (dotted line) and pH 5.4 (dashed line). Data are from n = 3-5 cells. The inset illustrates 10 µM GABA-activated currents at pH 7.4 and pH 5.4 obtained from 12H267K receptors. Note the increased rate and extent of receptor desensitization. Calibration: 400 pA, 2 sec.

View larger version (21K): [in this window] [in a new window]   Figure 6.   Zn2+ concentration inhibition curves for 12 (), 12H267E (), and 12H267K () receptors determined by coapplication of Zn2+ with 10 µM GABA. The ordinate describes the response amplitude to GABA in Zn2+ as a percentage of the control response to GABA in the absence of Zn2+. After each Zn2+ application, full recoveries from inhibition were obtained. The data were obtained from n = 3-5 cells and were fitted using Equation 3, presented in Materials and Methods.

The lysine substitution at position 267 was performed because the side chain, with a projected p_Ka of 10, is positively charged at pH 7.4 (99.7% cationic) and also at pH 5.4 (100%). Therefore, this residue would already be protonated and could not participate in the modulation of GABA-activated currents between pH 5.4 and pH 7.4. In addition, Zn²⁺ inhibition should be markedly reduced, as observed with the 2^H267A substitution. However, the charged side chain presented by the lysine residue conferred unusual and profoundly different functional properties on the GABA_A receptor. First, this mutation did not ablate the H⁺ sensitivity of 10 µM GABA-activated currents, with potentiation by 52 ± 7% observed at pH 5.4 compared with pH 7.4 (EC₅₀: pH 7.4, 17.2 ± 7.4 µM; pH 5.4, 11 ± 1.9 µM; p < 0.05 compared with 12 wild type; n = 5) (Fig. 5B). Second, the lysine mutation further reduced the sensitivity to Zn²⁺, with the inhibition curve then defined by an IC₅₀ of 990 ± 116 µM (Fig. 6). Finally, the desensitization profile of the GABA-induced currents for 12^H267K receptors was markedly affected, with increased rates of desensitization and peak currents declining back to the baseline holding current, even during GABA application (Fig. 5B, inset). These results clearly emphasized the crucial nature of the identity of the residue at position 267 in the ion channel lining of the  subunit in shaping the response profile of the GABA_A receptor.

	DISCUSSION

TOP ABSTRACT INTRODUCTION MATERIALS AND METHODS RESULTS DISCUSSION REFERENCES

This study concludes that for 1i GABA_A receptors, H⁺-induced potentiation completely relies on a single histidine residue, H267, in  subunits; this residue resides at the external portal of the ion channel. Previous site-specific mutagenesis revealed that H267 also underpinned a substantial component of Zn²⁺ inhibition of GABA-activated responses, suggesting that it is important for the allosteric modulation of GABA_A receptor function.

The involvement of a histidine residue, conserved between the  subunit isoforms, in the regulation of GABA_A receptor function was suggested from the similar pH titration profiles for 11 and 12 receptors. This was also supported by the unequivocal effect of DEPCs completely removing any potentiation by H⁺ on 11 and 12 receptors. Interestingly, this action unveiled a small inhibitory effect of H⁺ that was observed only at low pH values with a projected p_Ka approaching 4, indicating the involvement of aspartate and/or glutamate residues. Mutagenesis subsequently confirmed that only H267 in the  subunits was critically involved in H⁺-induced potentiation. Because H⁺ can compete and prevent the inhibitory effect of Zn²⁺ on recombinant and native GABA_A receptors (Krishek et al., 1998) and because H267 has already been suggested to be an important coordinating residue for Zn²⁺ inhibition at 1i subunit GABA_A receptors (Wooltorton et al., 1997a; Horenstein and Akabas, 1998), we suggest that protonation of this residue is likely to underlie the potentiating effect of H⁺ on 1i GABA_A receptors. Furthermore, mutating H267 does not affect GABA activation of the receptor, thus suggesting that this residue is not part of the main agonist signal transduction process.

To further examine the role of H267, we substituted this histidine for glutamate and lysine to vary the charge in the side chain. Although inclusion of the carboxyl side chain for 12^H267E abolished H⁺-induced potentiation, Zn²⁺ inhibition was reduced to an extent similar to that observed after alanine substitution. This suggested that the orientation of the side chain in this position must be important, because glutamate, by virtue of electron donation, would be expected to bind Zn²⁺ and thus substitute, at least partly, for histidine. However, it is clear that this did not occur. The lysine substitution, however, yielded unexpected results, with the H⁺-induced potentiation only partly reduced when we might have expected abolition; however, the level of Zn²⁺ inhibition was markedly reduced, far more than expected from the results with the neutral alanine substitution at position 267. We had predicted that the H⁺-induced potentiation would be ablated because of the positively charged lysine side chain (p_Ka 10); however, if H⁺ is protonating the lysine residue at position 267, this residue cannot be 100% charged, and the p_Ka must be reduced by at least 2000- to 3000-fold (2-3 pH units) to ~7 for this to occur. Such a shift in p_Ka has been reported in the inward rectifier potassium channel, K_ir 1.1, where an intracellular pH-sensitive lysine can be protonated at pH 5.4 (Schulte et al., 1999). Such an effect can be achieved only if other positively charged residues (e.g., arginines and lysines) electrostatically shield the proton-sensitive lysine from basal H⁺ (Schulte et al., 1999). Indeed, both R269 and K274 (conserved throughout the GABA_A receptor , , and  subunit families) lie close, at least in primary sequence order, to H267K. In the  subunits, such proximity might act to shield the lysine at 267 from H⁺. The results with Zn²⁺ and H267K could be explained by the action this residue has in further disrupting, beyond that achieved with H267A, the allosteric mechanism for Zn²⁺ inhibition.

Although mutagenesis cannot unequivocally indicate the presence of a binding site on a receptor, the lack of any relatively comparable effects on H⁺ and Zn²⁺ modulation by E267 and K267 suggests that this position is probably not involved in a common signal transduction pathway that is "downstream" of ion binding sites. Furthermore, because H⁺ and Zn²⁺ have opposite effects on GABA_A receptor function, it is also unlikely that H267 is an element in part of a common signal transduction mechanism.

The identification of H267 as a potential binding site for H⁺ and Zn²⁺ presented a curious problem. If these positively charged ions are capable of binding to the same residue, how can they induce completely opposite effects of inhibition (Zn²⁺) and potentiation (H⁺) on GABA_A receptor function? The key to this question may involve the different ways in which H⁺ and Zn²⁺ interact with the imidazole groups of histidines. The addition of H⁺ will cause protonation of individual imidazole rings, independent of any other residues. This protonation and subsequent potentiation of the GABA response might involve electrostatic repulsion between adjacent imidazole groups on the  subunits in the channel (two to three depending on subunit stoichiometry), causing a local conformational change in the receptor. In contrast, zinc ions will form a coordinated bond with one of the imidazole ring nitrogens and at least three other residues (which could include other imidazole rings from additional  subunits in the receptor) and an activated water molecule. This would be typical of many Zn²⁺ binding sites in metalloenzymes (Vallee and Auld, 1990). The coordination of Zn²⁺ with H267 would inhibit receptor function allosterically rather than by a physical channel block mechanism (Legendre and Westbrook, 1991; Smart, 1992; Gingrich and Burkat, 1998). This coordination, compared with protonation, would not polarize adjacent imidazole rings. Thus, mechanistically, at the level of amino acid side chains, it is conceivable that H⁺ and Zn²⁺ could cause differential effects on channel function, but the precise movements of amino acids will need to await x-ray crystallographic study. A similar scenario accounts for the differential effects of Zn²⁺ and H⁺ on the M2 ion channel from influenza A virus (Okada et al., 2001).

Overall, these data indicate the importance of a single TM2 residue in the ion channel lining of the  subunits for H⁺-induced modulation of GABA_A receptor function and also suggest that H267 could be a potential binding site for at least two types of cations.

	FOOTNOTES

Received Jan. 31, 2002; revised April 18, 2002; accepted April 18, 2002.

This work was supported by the Medical Research Council. We thank Robert Harvey for providing 1/2^H267A clones.

Correspondence should be addressed to T. G. Smart, Department of Pharmacology, University College London, WC1E 6BT, UK. E-mail: t.smart{at}ucl.ac.uk.

M. E. Wilkin's and A. M. Hosie's present address: Department of Pharmacology, University College London, London, WC1E 6BT, UK.

	REFERENCES

TOP ABSTRACT INTRODUCTION MATERIALS AND METHODS RESULTS DISCUSSION REFERENCES

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• Connolly CN, Krishek BJ, McDonald BJ, Smart TG, Moss SJ (1996) Assembly and cell surface expression of heteromeric and homomeric gamma-aminobutyric acid type A receptors. J Biol Chem 271:89-96[Abstract/Free Full Text].
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