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The Journal of Neuroscience, September 1, 2002, 22(17):7326-7330
BRIEF COMMUNICATION
Differential Resynchronisation of Circadian Clock Gene Expression within the Suprachiasmatic Nuclei of Mice Subjected to Experimental Jet LagA. B. Reddy1, *, M. D. Field2, *, E. S. Maywood1, and M. H. Hastings1 1 Medical Research Council Laboratory of Molecular Biology, Division of Neurobiology, University of Cambridge, Cambridge, CB2 2QH, United Kingdom, and 2 Department of Anatomy, University of Cambridge, Cambridge, CB2 3DY, United Kingdom
ABSTRACT
TOP
ABSTRACT
INTRODUCTION
Disruption of the circadian timing system arising from travel between time zones ("jet lag") and rotational shift work impairs mental and physical performance and severely compromises long-term health. Circadian disruption is more severe during adaptation to advances in local time, because the circadian clock takes much longer to phase advance than delay. The recent identification of mammalian circadian clock genes now makes it possible to examine time zone adjustments from the perspective of molecular events within the suprachiasmatic nucleus (SCN), the principal circadian oscillator. Current models of the clockwork posit interlocked transcriptional/post-translational feedback loops based on the light-sensitive Period (Per) genes and the Cryptochrome (Cry) genes, which are indirectly regulated by light. We show that circadian cycles of mPer expression in the mouse SCN react rapidly to an advance in the lighting schedule, whereas rhythmic mCry1 expression advances more slowly, in parallel to the gradual resetting of the activity-rest cycle. In contrast, during a delay in local time the mPer and mCry cycles react rapidly, completing the 6 hr shift together by the second cycle, in parallel with the activity-rest cycle. These results reveal the potential for dissociation of mPer and mCry expression within the central oscillator during circadian resetting and a differential molecular response of the clock during advance and delay resetting. They highlight the indirect photic regulation of mCry1 as a potentially rate-limiting factor in behavioral adjustment to time zone transitions.
Key words: entrainment; period; cryptochrome; circadian; suprachiasmatic nuclei; jet lag
INTRODUCTION
Disruption of circadian timing during jet lag and rotational shift work acutely impairs performance and causes chronic health problems (Siegel et al., 1969
; Costa, 1996
Identifying the origin of inertia during resetting has theoretical and practical relevance. Formal analyses indicate, paradoxically, that the phase response curve, an indication of directly light-responsive elements of the oscillator, resets rapidly, within 2 hr (Best et al., 1999
This model has important implications for understanding adjustment to time zone transitions. Whereas the Per1 and Per2 are acutely upregulated by nocturnal light (Shigeyoshi et al., 1997
MATERIALS AND METHODS
All experiments were licensed by the Home Office (United Kingdom). Adult male CD1(ICR) mice (6 weeks; Harlan Olac, Bicester, UK) were housed individually or in groups, with food and water available ad libitum, in light-proof, ventilated chambers, under a 12 hr bright white light (L) (220 µW/cm2), 12 hr dim red light (DR) (<5 µW/cm2) cycle. Activity-rest cycles were monitored with passive infrared movement detectors or running wheels linked to an automated recording system (Dataquest IV; DSI, Frankfurt, Germany). Lights-off was defined as zeitgeber time (ZT) 12. Circadian time (CT) 12 indicated activity onset under continuous DR.
Experiment 1. Groups of animals were killed by cervical dislocation hourly on the baseline day. Others were subjected to an acute advance in the lighting schedule (advanced lights-on) and were killed hourly for 18 hr intervals, commencing at the time of new lights-on, over the first, third, and eighth days after the shift.
Experiment 2. Mice were subjected to a 6 hr delay in the time of lights-on and lights-off and sampled in groups every hour for 44 hr. In both experiments wheel-running was recorded in five additional mice to monitor behavioral re-entrainment.
Frozen brains were processed for in situ hybridization as described previously (Field et al., 2000
RESULTS
Advance resetting is accompanied by dissociation of SCN mPer and mCry expression
Mice entrained to a 12 hr light/dark cycle (12L:12D) became active at lights-off (Fig. 1a,c). After a 6 hr advance in the photoschedule, the activity-rest cycle exhibited progressive re-entrainment. After 3 d, activity onset had advanced by 4.17 ± 0.25 SEM hr. Stable re-entrainment was only achieved after 1 week.
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Figure 1. Re-entrainment of mouse activity-rest cycles to shifts of the photoschedule. a, b, Representative double-plotted actograms of mice subjected to 6 hr phase advance or phase delay on days marked by asterisk. Top bars indicate initial L:D cycle; bottom bars below indicate shifted cycle. c, Activity onset (mean + SEM; n = 5) after the 6 hr advance (left panel) or delay (right panel).
Clock gene expression within the SCN of mice entrained to 12L:12D was equivalent to that reported previously. mPer1 mRNA peaked around ZT06, and mPer2 and mCry1 mRNA around lights-off (ZT12) (Fig. 2a). On day 1 of the advanced schedule, light onset occurred in the middle of the former night, original ZT18. mPer1 mRNA was strongly induced, especially in the ventral SCN (Figs. 2a, 3a) (ANOVA over first 6 hr of day 1, treatment and time effects, p < 0.01; interaction p < 0.01). Despite the continuing illumination, however, mPer1 expression returned to control levels within 2-3 hr. For the remainder of day 1, the endogenous cycle of mPer1 expression was not significantly different from that of the control cycle. Acute induction of mPer2 mRNA also occurred on day 1, peaking in the first 4-5 hr of illumination (Figs. 2a, 3a), followed by a decline to control levels (ANOVA treatment effect, and treatment × time interaction, p < 0.01). The spontaneous cycle of mPer2 expression for the remainder of day 1 was not significantly different from the control cycle. In contrast to mPer, mCry1 in the SCN was not acutely affected by the advanced lighting (Figs. 2a, 3a). Although there was a trend for mCry1 expression to be advanced slightly, this was not significant.
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Figure 2. Re-entrainment of SCN gene expression cycles to an advanced lighting schedule. a, Intensity of mRNA hybridization signals for mPer1, mPer2, or mCry1 in SCN before (baseline) and on the first day of the advance phase shift. Baseline data are plotted as observed hourly values (closed circles, mean of 3 mice/hr), and as the three-point moving average (solid line, double-plotted on right as dotted line for clarity). Data from day 1 of the shift are single-plotted on the right (open circles, solid line), represented by the observed hourly mean (3 mice/hr, for the first 6 hr of light exposure) or as the three point moving average for the hourly means (3 mice/hr) for the subsequent 12 hr. b, Intensity of SCN mRNA hybridization signals before (baseline data, dotted line) and on the third (closed circles, dashed line) and eighth (open circles, solid line) days of the advance phase shift. Plotted as the three point moving average for the hourly means (3 mice/hr).
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Figure 3. Induction of circadian clock genes in SCN of phase-shifted mice. Representative dark-field autoradiograms of in situ hybridization. A, mPer and mCry1 expression in mice entrained to 12L:12D and sampled in darkness 1(mPer1) or 3 hr (mPer2, mCry1) after lights-off, or after acute 6 hr phase advance of the L:D cycle. b, mPer2 and mCry1 expression 3 d after a 6 hr advance, sampled 2, 12, or 17 hr after lights-on. Note dissociation of Per and Cry expression. Images are derived from the same animal at each time. c, mPer and mCry1 expression in mice subjected to an acute 6 hr delay of the light/dark cycle. Mice were sampled in darkness at ZT18 or after 6 hr of light exposure between former ZT12 to ZT18. Note coordinate upregulation of both mPer2 and mCry1 mRNA. Induction to mPer1 was weak and restricted to the ventral SCN. Images for the three mRNAs are derived from the same animal at either time. Representative coronal sections immunostained for mPER or mCRY. d, Mice entrained to 12L:12D, sampled at ZT02, or subjected to an acute 6 hr phase advance to lights-on (now on at original ZT18) and sampled after 8 hr of illumination (new ZT08). e, Mice entrained to 12L:12D, sampled at ZT22, or subjected to an acute 6 hr phase delay (lights now off at original ZT18) and sampled 4 hr after the end of illumination (original ZT22, new ZT16).
Dissociation of mPer and mCry1 expression was again apparent on the third day of the advance. Expression of both mPer genes filled the entire light phase, in contrast to the limited induction on day 1 (Fig. 2b). Consequently, their rhythmic expression on day 3 was not significantly different from day 8. In contrast, the peak of mCry1 expression on day 3 was in an intermediate position between the baseline day and day 8 (Fig. 2b). Moreover, the profile of mCry1 mRNA was distorted insofar as levels remained high for several hours after the peak of expression. There was no clear fall in mCry1 mRNA levels after lights-off, in contrast to that seen in entrained mice. This dissociation of gene cycles, with oppositely phased peaks and troughs, is apparent in Figure 3b. Two hours after lights-on, mPer2 levels were high, whereas mCry1 expression was basal in the same SCN. Both genes were highly expressed after 12 hr of illumination. Five hours after lights-off, however, mPer2 levels were basal, whereas mCry1 mRNA remained high. By day 8 the rhythm of mCry1 expression had re-entrained to the advanced lighting cycle, consistent with the completed behavioral response, and was again synchronized to the mPer2 cycle. Three-way ANOVA revealed a highly significant interaction between gene, time of day, and day of treatment, confirming that these two genes exhibited differential responses to time zone advances. A similar, highly significant interaction was also observed for the comparison between mPer1 and mCry1 (data not shown), demonstrating that the cycles of expression of these gene products were also dissociated during advance resetting.
The contrast between the rapid response of mPer genes and the gradual advance of mCry1 to the shifted photoschedule is consistent with the model in which mCry is reset through light-dependent changes in mPER2 and thence of mBMAL1. We examined mPER expression in the SCN of control mice at ZT02 or mice sampled at an equivalent time, 8 hr after the advanced light onset on day 1 (n = 3 both groups). Nuclear mPER1 and mPER2 expression in control, nonshifted animals exhibited the very restricted pattern of immunoreactive cells in the dorsal SCN, typical of this phase (Fig. 3d) (mPER1 108.6 ± 7.0, mPER2 160.3 ± 25.8, cells per SCN section, mean + SEM). In contrast, mice subjected to the advance expressed significantly higher mPER1-IR (321.7 ± 32.5) and mPER2-IR (618.7 ± 74.5), predominantly in the ventral SCN, confirming translation of the acutely induced mRNAs.
Adjustment to a delay in local time is accompanied by coordinated SCN expression of mCry1 and mPer2
Our working hypothesis predicted that coordinate and rapid readjustment of the Per and Cry genes would accompany rapid behavioral resetting during phase delays. We therefore examined SCN clock gene expression in mice subjected to a 6 hr delay of the photo schedule. The resulting delay in the rhythm of wheel-running was completed within 2 cycles, with little evidence of gradual resetting (Fig. 1b,c).
The delayed photo schedule had little immediate impact on the expression of mPer1 (Figs. 3c, 4a). Levels were already falling because the delayed lighting illuminated the original dark phase (ZT12-ZT18). There was limited induction of mRNA in the ventralmost SCN, but less marked than accompanied the advance. This was mirrored by only sporadic mPER1 protein in the ventral SCN 4 hr after the delayed illumination ended (Fig. 3e). Nevertheless, on the following cycle, the profile of mPer1 expression was not significantly different from the projected 6 hr delay of the baseline cycle. The phase delay was complete by the second night (Fig. 4).
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Figure 4. Rapid readjustment of rhythmic expression of mPer and mCry mRNAs in SCN of mice subjected to an acute 6 hr phase delay. Data plotted as hourly means (circles; 3 mice/hr) and as a three-point moving average for the hourly data (solid line). For comparison, the baseline data are plotted in their original phase (dotted line, left panel) and with a predicted 6 hr delay on the second cycle after the shift (dotted line, right panel). Bars depict original (top bar) and shifted photoschedules (bottom bars). Arrow indicates time of exposure to unanticipated nocturnal light.
In contrast to mPer1, the peak of mPer2 expression in the SCN was dramatically extended during illumination of the original ZT12 and ZT18 (Figs. 3c, 4). Levels then fell after lights-off. On the second day and night after the shift, the 6 hr phase delay to the expression of mPer2 had been completed. Exposure to a delayed photoschedule also upregulated the expression of mCry1 in the SCN. On the first cycle of the delay there was a pronounced extension to the mCry1 peak, and levels did not fall until after the new time of lights-off (Figs. 3c, 4). This was mirrored by an extension in mCRY1 expression. In control mice sampled at original ZT22, sporadic mCRY1-IR nuclei were located in the dorsal SCN (98 ± 12 cells per section; n = 3). During the 6 hr delay, mCRY1-IR nuclei were abundant across the SCN, including the ventral retinorecipient regions (389 ± 62; n = 3; p < 0.01 vs controls) (Fig. 3e). As for mPers, the rhythm of mCry1 on the second cycle after the shift matched that predicted by an acute 6 hr delay of the baseline profile.
The mechanism underlying mCry1 induction is not clear, although the simultaneous and prolonged rise in mPer2 mRNA levels was translated into an extended expression of mPER2 protein. In control mice at ZT22, mPER2-IR nuclei were infrequent and predominantly in the dorsal SCN (mean ± SEM; 68 ± 7 cells per section; n = 3). In mice subjected to the delay, mPER2-IR nuclei were abundant across the SCN (509 ± 25; n = 3; p < 0.01 vs controls) (Fig. 3e).
These results demonstrate that acute phase delays of the circadian system are associated with coordinate shifts of both components of the oscillator, mPer and mCry. This rapid mCry response may underlie the rapid adjustment of behavioral rhythms to delays in local time. It contrasts markedly with gradual advance resetting characterized by dissociation of rapidly adjusting mPer and slowly adjusting mCry1.
DISCUSSION
During phase advances of the SCN clockwork the mPer and mCry genes dissociate as the mPers react rapidly to the new lighting regime, whereas the mCry1 cycle lags behind, advancing in phase with the activity-rest rhythm. Conversely, rapid behavioral responses during delay resetting are accompanied by coordinate, rapid induction of both mPer and mCry expression in the SCN. The degree of molecular dissociation in the SCN accompanying time zone transitions is therefore a function of the direction of resetting and arises from the differential sensitivity of core circadian genes to light.
The differential speed of advance and delay resetting corresponds directly to previous reports in animals exposed either to an acute shift in the complete lighting cycle (Yamazaki et al., 2000
The rapid readjustment of mPer1 to advanced lighting is consistent with an earlier report in C57BL/6 mice sampled every 6 hr (Sun et al., 1997
We therefore propose an alternative, although not necessarily contradictory, model in which inertia during time zone adjustment occurs within the SCN, and that it arises from the slow reaction of the mCry1 gene, a consequence of its indirect photic regulation. This model does not, of course, preclude further elaboration, and the rate of resetting may also be limited by intercommunication between SCN neurons, especially light-responsive and nonresponsive populations. Moreover, lags in signaling between the SCN and dependent peripheral tissues via slow neuroendocrine pathways will also contribute to the observed slow resetting of peripheral tissues (Yamazaki et al., 2000
Our model is supported by the temporal correlation between behavioral resetting and readjustment of the SCN mCry1 cycle. During both gradual advances and immediate delays, the shift of the mCry1 loop matched that for the overt activity-rest rhythm, suggesting that the response of mCry1 in the SCN may be sufficient to explain gradual resetting of locomotor behavior. Although not tested in the current study, it is strongly predicted that progressive advances of mCry, dissociated from mPer expression, would also accompany gradual advance resetting arising from single light pulses presented under free-running conditions. mCRY proteins are the principal mediators of transcriptional suppression within the clockwork and also on clock-controlled genes (Kume et al., 1999
Photic regulation of mCry is presumably a consequence of altered expression of mPER2, although the relative contribution of mPer1 and mPer2 to resetting is not known. Antisense oligonucleotides to mPer1 can block resetting (Akiyama et al., 1999
Why does mCry1 shift rapidly during delays but not during advances? Acute light pulses do not affect mCry expression, although sustained exposure to light clearly can. One obvious difference is that advances follow exposure to light during late subjective night, when mCry1 expression is spontaneously low. Delay resetting with "dusk" light occurs when mCry1 levels are high. The molecular substrate for mCry1 expression may be easier to sustain once spontaneously active, rather than to activate de novo in a cellular environment that will not support spontaneous (i.e., circadian) mCry1 expression. Further clarification of the protein:DNA interactions driving mCry1 expression is necessary to address this, although mPER2 is likely to be an important contributor. In delay resetting, mPER2 protein expression is spontaneously high and sustained by exposure to light, and photic activation of mCry1 and mPer2 occurred over the same 6 hr of light exposure (this study), raising the possibility that mPER2 has an effect on mCry1 expression independently of its proposed regulation of mBMAL1. With the advance shifts, mPER2 protein levels are initially low and may take some time to increase in response to light, thereby compromising induction of mCry1.
In conclusion, our results identify mPer genes, in particular mPer2, as critical components of the photic entrainment pathway, whereas mCry1 appears to be a rate-limiting factor in the expression of behavioral resetting. As such, this gene may be an important therapeutic target for management of circadian disorders such as sleep disturbance, jet lag, and shift work.
FOOTNOTES Received Feb. 26, 2002; revised June 19, 2002; accepted June 19, 2002.
* A.B.R. and M.D.F. contributed equally to this work.
We thank J. Bashford for expert assistance with photomicrography, Profs. H. Okamura (Kobe University, Japan) and S. M. Reppert, (University of Massachusetts, Boston, MA) for plasmid templates, and W. Rashband (National Institutes of Health, Bethesda, MD) for Image software.
Correspondence should be addressed to Michael H. Hastings, Medical Research Council Laboratory of Molecular Biology, Hills Road, Cambridge, CB2 2QH, UK. E-mail: mh105{at}cus.cam.ac.uk.
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