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The Journal of Neuroscience, September 1, 2002, ():

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Ionomycin-induced translocation of myrVILIP-1-GFP in COS-7 cells and complete reversal after calcium depletion with EGTA. Living COS-7 cells expressing myrVILIP-1-GFP were stimulated by addition of 2 µM ionomycin (+IONOMYCIN) leading to increased fluorescence at the plasma membrane and intracellular structures. The ionomycin-dependent localization of myrVILIP-1-GFP was reversed after addition of 5 mM EGTA (+EGTA) to lower the calcium concentration in the incubation buffer. Fluorescence of myrVILIP-1-GFP-transfected COS-7 cells was monitored by taking images every 30 seconds using a Leica DMR upright fluorescence microscope and a Visitron CCD camera.

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