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The Journal of Neuroscience, October 1, 2002, 22(19):8391-8401

ER-X: A Novel, Plasma Membrane-Associated, Putative Estrogen Receptor That Is Regulated during Development and after Ischemic Brain Injury

C. Dominique Toran-Allerand^{1, 3, 5, 6}, Xiaoping Guan^{2, 6}, Neil J. MacLusky^{2, 6}, Tamas L. Horvath⁷, Sabrina Diano⁷, Meharvan Singh^{2, 6}, E. Sander Connolly Jr⁴, Imam S. Nethrapalli^{2, 6, *}, and Alexander A. Tinnikov^{2, 6, *}

Departments of ¹ Anatomy and Cell Biology, ² Obstetrics and Gynecology, ³ Neurology, and ⁴ Neurosurgery, and Centers for ⁵ Neurobiology and Behavior and ⁶ Reproductive Sciences, Columbia University College of Physicians and Surgeons, New York, New York 10032, and ⁷ Department of Obstetrics and Gynecology, Yale University School of Medicine, New Haven, Connecticut 06510

	ABSTRACT

TOP ABSTRACT INTRODUCTION MATERIALS AND METHODS RESULTS DISCUSSION REFERENCES

We showed previously in neocortical explants, derived from developing wild-type and estrogen receptor (ER)- gene-disrupted (ERKO) mice, that both 17- and 17-estradiol elicit the rapid and sustained phosphorylation and activation of the mitogen-activated protein kinase (MAPK) isoforms, the extracellular signal-regulated kinases ERK1 and ERK2. We proposed that the ER mediating activation of the MAPK cascade, a signaling pathway important for cell division, neuronal differentiation, and neuronal survival in the developing brain, is neither ER- nor ER- but a novel, plasma membrane-associated, putative ER with unique properties. The data presented here provide further evidence that points strongly to the existence of a high-affinity, saturable, ³H-estradiol binding site (K_d, ~1.6 nM) in the plasma membrane. Unlike neocortical ER-, which is intranuclear and developmentally regulated, and neocortical ER-, which is intranuclear and expressed throughout life, this functional, plasma membrane-associated ER, which we have designated "ER-X," is enriched in caveolar-like microdomains (CLMs) of postnatal, but not adult, wild-type and ERKO neocortical and uterine plasma membranes. We show further that ER-X is functionally distinct from ER- and ER-, and that, like ER-, it is re-expressed in the adult brain, after ischemic stroke injury. We also confirmed in a cell-free system that ER- is an inhibitory regulator of ERK activation, as we showed previously in neocortical cultures. Association with CLM complexes positions ER-X uniquely to interact rapidly with kinases of the MAPK cascade and other signaling pathways, providing a novel mechanism for mediation of the influences of estrogen on neuronal differentiation, survival, and plasticity.

Key words: caveolae/caveolar-like microdomains; 17-estradiol; 17-estradiol; ERK1/2; ERKO; brain; neocortex; uterus; development

	INTRODUCTION

TOP ABSTRACT INTRODUCTION MATERIALS AND METHODS RESULTS DISCUSSION REFERENCES

Two mammalian estrogen receptor (ER) genes are now known, encoding, respectively, ER- (~67 kDa) (White et al., 1987), which mediates many of the known transcriptional actions of estrogen in the brain, and the more recently cloned ER- (Kuiper et al., 1996) (60 kDa in mouse ovary) (Fitzpatrick et al., 1999), whose neural role is less well defined. A third, more distantly related member of the ER family, ER-, was recently cloned in teleosts only (Hawkins et al., 2000). ER- and ER- appear to be complementary but not redundant. Under steady-state conditions they are predominantly intranuclear and differ with respect to the homology of their functional domains, binding affinities, and ligand specificities (Kuiper et al., 1997). ER- and ER- act as ligand-inducible transcriptional enhancers (Landers and Spelsberg, 1992; Beato and Klug, 2000), binding to cognate estrogen response elements (EREs) in DNA to regulate gene expression. Their spatiotemporal expression and distribution differ with developmental stage. Thus, although neocortical ER- is present throughout life (Shughrue et al., 1997), neocortical ER- expression is developmentally regulated and normally expressed at high levels only during neocortical differentiation (Shughrue et al., 1990, 1997), suggesting a more restricted developmental role.

Some responses to estradiol cannot be attributed to ER- or ER- (Singh et al., 1999, 2000), such as the ability of estrogen to regulate non-ERE-containing genes (Sukovich et al., 1994) and the very rapid (seconds to minutes) effects of estrogen (Kelly et al., 1978; Chiaia et al., 1983; Garcia-Segura et al., 1987; Migliaccio et al., 1993). Whereas such rapid responses appear inconsistent with direct transcriptional modulation via intranuclear receptors, they could be explained by the presence of plasma membrane-associated ERs that may be coupled to signal transduction pathways, typically associated with rapid activation by growth factors.

Neurotrophin activation of the mitogen-activated protein kinase (MAPK) cascade is mediated by cognate transmembrane receptors associated with caveolar-like microdomains (CLMs) of neuronal plasma membranes, the neuronal homologs of caveolae found in most cell types other than neurons (Huang et al., 1999). Caveolae/CLMs form important signaling modules that compartmentalize, modulate, and integrate growth factor-induced signaling events at the cell surface (Anderson, 1998; Okamoto et al., 1998). Like the neurotrophins, estrogen is an important neural trophic factor throughout life, with influences on neuronal differentiation (Toran-Allerand, 1976, 1980), survival (Green and Simpkins, 2000; Garcia-Segura et al., 2001), and plasticity (Matsumoto and Arai, 1981). 17-estradiol activates many signaling kinases, including protein kinase C (PKC) (G. Sétáló, Jr. and C. D. Toran-Allerand, unpublished observations), c-src (Nethrapalli et al., 2001), and members of the MAPK cascade (Watters et al., 1997; Singh et al., 1999, 2000). Rapid and sustained activation of cytoplasmic ERK1/2 is followed by nuclear translocation of phosphorylated ERK (Sétáló et al., 2001). We have proposed (Singh et al., 1999, 2000) that the ER mediating estrogen-induced activation of ERK1/2 in the developing brain is neither ER- nor ER- and have hypothesized that, like neurotrophin receptors, this ER might be associated with CLMs (Toran-Allerand, 2000). Here we provide evidence for the existence of a novel and unique, plasma membrane-associated (CLM-associated) putative ER that we have designated "ER-X" (Toran-Allerand, 2000).

	MATERIALS AND METHODS

TOP ABSTRACT INTRODUCTION MATERIALS AND METHODS RESULTS DISCUSSION REFERENCES

All animal experiments were conducted in a humane manner, and animals were maintained according to protocols approved by the Institutional Animal Care and Use Committee at Columbia University. To identify and characterize ER-X, we analyzed, by immunoprecipitation, Western blotting and both light and electron microscopy, cell lysates, detergent-free, highly purified CLM preparations (Smart et al., 1995), plasma membranes, postnuclear supernatants (PNS), and tissue sections obtained from postnatal day 7 (P7) and adult wild-type and ER- gene-disrupted (ERKO) neocortex and uterus.

Mice. Wild-type and ERKO mice were obtained from our breeding colony from matings of C57BL/6J × 129 mice heterozygous (+/) for the ER- gene disruption (Lubahn et al., 1993) and identified by genotyping (Singh et al., 2000) as either wild-type (+/+) or homozygous (/) for the disruption.

Genotyping. Tail snips were obtained from P3-P4 pups and used for genotyping, as previously described (Singh et al., 2000). Briefly, tissues were digested with Proteinase K at 56°C for 90 min, followed by a 99°C incubation for 10 min. The samples were then vortexed vigorously, and insoluble material was pelleted in a microfuge. Supernatants were used in a PCR reaction that used one primer pair [primer 1: 5'-CGG TCT ACG GCC AGT CGG GCA TC-3'; primer 2: 5'-GTA GAA GGC GGG AGG GCC GGT GTC-3'] for the ER- gene product (product size, 239 bp), and one primer pair [primer 2 from above with NEO Primer: 5'-GCT GAC CGC TTC CTC GTG CTT TAC-3'] for the neomycin insert-containing gene product (product size, 790 bp). The PCR program was performed as follows: 1 cycle at 94°C for 3 min, 30 cycles of 94°C for 45 sec, 62°C for 1 min, and 72°C for 1 min 40 sec, followed by a final extension cycle of 72°C for 7 min. Products were analyzed by agarose gel electrophoresis. Wild-type animals revealed the smaller 239 bp band, homozygous knock-outs (ERKO) showed the larger 790 bp band, and heterozygotes displayed both bands.

Neocortical cultures. Organotypic explant cultures, obtained from 360 µm hemicoronal slices of the frontal and cingulate neocortex of P2 wild-type and ERKO mice (day of birth = P1), were explanted onto collagen-coated, poly-D-lysine precoated coverslips and maintained in roller tube culture with gonadal steroid-deficient (gelding serum) and phenol red-free nutrient medium, as previously described (Singh et al., 1999, 2000). The nutrient medium was supplemented with 17-estradiol (2 nM; Sigma, St. Louis, MO) for 1 week, to optimize the development of CNS cultures from estrogen target regions (C. D. Toran-Allerand, unpublished observations).

Immunoprecipitation and Western blot analysis. Tissues were harvested into protease and phosphatase inhibitor-containing lysis buffer (50 mM Tris-base, pH 7.4, 150 mM NaCl, 10% glycerol, 1 mM EGTA, 1 mM Na₃VO₄, 5 µM ZnCl₂, 100 mM NaF, 10 µg/ml aprotinin, 1 µg/ml leupeptin, 1 mM PMSF, and 1% Triton X-100) and prepared for immunoprecipitation and polyacrylamide gel electrophoresis, as previously described (Singh et al., 1999, 2000). Immunoprecipitation was performed, using an indirect technique with magnetic Dynabead separation (Dynal ASA, Oslo, Norway). All procedures were performed at 4°C. In brief, P7 wild-type and ERKO cerebral cortices were homogenized by passing the sample eight times through a syringe fitted with a 20-gauge needle. The homogenate was centrifuged at 100,000 × g at 4°C for 15 min, and the protein concentration of the supernatant was determined (Lowry's method; Bio-Rad Detergent Compatible Protein Assay kit; Bio-Rad, Hercules, CA). For coimmunoprecipitation experiments, detergent was omitted from the lysis buffer. Depending on the species of the antibodies to be used, the clarified lysates were precleared with either anti-mouse or anti rabbit IgG-coated Dynabeads to reduce nonspecific antibody-antigen binding. For immunoprecipitation of ER-X, the precleared lysates, recovered from the supernatant, were then incubated at 4°C for 12-24 hr with gentle shaking on a Nutator with 6F11, a mouse monoclonal ER antibody raised against the full-length mouse ER- molecule, which has proven to be optimal for immunoprecipitation of ER-X (Novocastra; Vector Laboratories, Burlingame, CA; 1:50-1:100). Primary antibody incubation was followed by the addition of anti-mouse IgG-coated Dynabeads for 3 hr to capture and precipitate the antibody-antigen complexes. The ER antibodies and coimmunoprecipitated proteins were separated from the Dynabeads by the addition of 1× sample loading buffer, containing 5% -mercaptoethanol, and boiling for 5 min. The Dynabeads were removed from the supernatant, using Dynal Magnetic Particle concentrators. The immunoprecipitated proteins were boiled at 95-100°C for 5 min, and 300-500 µg samples were loaded onto 10% SDS-PAGE gels and separated based on molecular size. Prestained rainbow markers (Bio-Rad) were used as molecular mass standards. The gels were then electroblotted onto polyvinylidene difluoride (PVDF) membranes.

Immunodetection of the protein of interest was performed by first blocking the membrane in 5% nonfat dry milk (Carnation; Nestle USA) in TBS-Tween 20 (10 mM Tris-base, 150 mM NaCl, and 0.2% Tween 20, pH 8.0), followed by addition of the primary antibody. Wherever feasible, the PVDF membranes were probed with antibodies different from those used for immunoprecipitation to maximize the specificity of the immunoreactive product obtained. For ER-X in particular, we used either of two antibodies highly specific for ER-: one specific for the ligand binding domain (LBD) of ER- (MC20; 1:500; Santa Cruz Biotechnology, Santa Cruz, CA) and the other raised against amino acids 586-600 of the C terminus of ER- (C1355; 1:2000; Friend et al., 1997; Upstate Biotechnology, Lake Placid, NY). Both antibodies recognize ER-X on Western immunoblots and by immunohistochemistry, but C1355 is not effective for immunoprecipitation. ER- was identified with antibodies directed against the LBD of ER- (1:250; Zymed, South San Francisco, CA). Negative controls to test for the specificity of the interactions were run in parallel and were performed by immunoprecipitation of the precleared protein lysates with preimmune mouse IgG and subsequently probed with the appropriate antibody. Additionally, a control peptide or lysate (uterus, ovary) was always used as a positive control to verify the identity of the band in the experimental lanes. The specificity of the signal was determined by the apparent molecular weight (MW) of the protein detected.

Antibody binding to protein was detected, using a secondary antibody conjugated to horseradish peroxidase (1:40,000; Pierce, Rockford, IL), and visualized autoradiographically on film, using enzyme-linked chemiluminescence (ECL; Amersham Biosciences, Arlington Heights, IL), as previously described (Singh et al., 1999, 2000). All blots were stripped and reprobed with the appropriate antibody to verify equal loading of protein across lanes and were analyzed densitometrically. For studies of ERK phosphorylation, the blots were first probed with phosphospecific ERK antibodies to detect phospho-ERK1/2 [phospho-p44/42 ^{MAP Kinase}, (Thr202/Tyr204) (1:1000; Cell Signaling, Beverly, MA)]. The same blot was reprobed for total (nonphosphorylated) ERK protein to verify equal loading [ERK-1 (C-16), or ERK-2 (C-14)] (1:1000; Santa Cruz Biotechnology). All antibodies were diluted in the blocking solution.

Densitometric analyses. Densitometric analyses of ERK protein levels were performed to ensure similar levels of protein loaded across lanes. Autoradiograms were scanned in triplicate, using an HP Scanjet 6200C (Hewlett Packard Company, Greeley, CO) and analyzed using Kodak 1D Image Analysis Software (Eastman Kodak, Rochester NY). Net intensity values were calculated by subtracting the background within the area measured for each band from the total intensity within this same measured area to account for any variation in background intensity across the film.

CLM preparation. Membrane fractions were prepared by adapting the detergent-free method of Smart et al. (1995). Briefly, pools of 40-50 P7 ERKO neocortices were homogenized in 20 mM Tricine, pH 7.8, buffer, containing 1 mM EDTA, 0.25 M sucrose, and 1 mM dithiothreitol (TESD buffer), then centrifuged at 1000 × g at 4°C for 10 min. The pellet was resuspended in TESD buffer, recentrifuged, and the supernatants were pooled. The combined supernatants were subjected to Percoll gradient fractionation in the same buffer to isolate the plasma membrane fraction. In some binding experiments (indicated below) Percoll-purified plasma membranes were used without further fractionation. For preparation of CLMs, plasma membranes were sonicated and further separated by centrifugation on a linear 20 to 10% OptiPrep (iodixanol) gradient (Nycomed Pharma A. S, Oslo, Norway). Based on their light-buoyant density, CLMs were separated and purified from non-CLMs, using two OptiPrep density gradients. The purity of the CLM preparation was verified immunologically by demonstrating the presence of CLM-enriched proteins: flotillin (1:250), PKC- and PKC- (1:1000) (Transduction Labs, Lexington, KY), and absence of the non-CLM-associated cytoskeletal protein paxillin (1:10,000; Transduction Labs). Electrophoretically separated CLMs on PVDF membranes were probed with antibodies specific for ER- [C1355, (Upstate Biotechnology); MC20 (Santa Cruz Biotechnology)]; ER- (Zymed); flotillin (Transduction Labs) and other caveolar-resident proteins: e.g., PKC- and PKC- (Transduction Labs) and noncaveolar-resident proteins: e.g., paxillin (Transduction Labs)].

Phosphorylation of ERK1/2 in CLM and non-CLM preparations. Phosphorylation of ERK1/2 in ERKO CLM and non-CLM preparations was examined following the method of Liu et al. (1997), except that basal medium Eagle (BME) was used in the place of MEM. Nine parts of CLM or non-CLM preparations were mixed with one part of 10× BME, pH 7.4, containing BSA 800 µg/ml, 10 mM NaF, 2 mM Na3VO4, leupeptin 100 µg/ml, soybean trypsin inhibitor 100 µg/ml, 10 mM MgCl₂, and 1 mM ATP. For MAP kinase kinase (MEK)1/2 inhibition of ERK activation, the CLMs were pretreated with the MEK1/2 inhibitor, U0126 (10 µM; Cell Signaling, Beverly, MA) for 30 min before pulsing them with the appropriate estradiol. Aliquots of ERKO CLMs and non-CLMs were exposed for 30 min at 37°C to either 17-estradiol (0.1 nM), 17-estradiol (10 nM), U0126 (10 µM) or a sham control and processed for ERK1/2 phosphorylation, using antibodies to phosphorylated p44/42^{MAP Kinase} (ERK1/2) (Thr202/Tyr204) (Cell Signaling Technology), as previously described (Singh et al., 1999, 2000).

Isolation of PNS. To increase the yield of ER-X and to test in a cell-free system whether the presence of ER- is inhibitory for ERK activation, as we had shown previously in neocortical cultures (Singh et al., 2000), we also studied PNS, a cell-free system that contains all the cell organelles except the nucleus. PNS was isolated from P7 wild-type and ERKO neocortices, according to the method of Smart et al. (1995). Three or four P7 wild-type and ERKO neocortices were homogenized, using a Teflon homogenizer in 1 ml of 20 mM Tricine, pH 7.8, buffer, containing 1 mM EDTA, 0.25 M sucrose, 10 µg/ml aprotinin, and 1 µg/ml leupeptin. The homogenate was centrifuged at 1000 × g at 4°C for 10 min. The supernatant obtained is the PNS. The pellet was resuspended in 500 µl of the homogenization buffer, recentrifuged, and the PNS obtained was pooled with the first PNS. ERKO and wild-type PNS were mixed with 10× phosphorylation buffer, and the MAPK assay was performed as described above. PNS samples were exposed to 17-estradiol (0.1 nM), 17-estradiol (10 nM), the ER--selective ligand propylpyrazole triol (PPT) (100 nM) (Stauffer et al., 2000) (a gift from J. A. Katzenellenbogen, University of Illinois, Champaign/Urbana), the MEK inhibitor U0126 (10 µM), BDNF (100 ng/ml), ethanol (0.001%), DMSO (0.001%), and a sham control; first, for 10 min at 4°C, followed by 10 min at 37°C.

Cholesterol depletion. To determine whether disruption of CLMs impairs estrogen activation of the MAPK cascade, neocortical explants were pretreated on P9 with the sterol binding agent Nystatin (50 µg/ml) (Sigma), a compound used extensively to document the association of growth factor receptors with caveolae/CLMs (Huang et al., 1999). This concentration of Nystatin has been shown to result in a significant reduction of cellular cholesterol content without appreciably affecting cell viability (Rothberg et al., 1990). P9 neocortical explants were exposed to Nystatin (50 µg/ml) (Sigma), BDNF (100 ng/ml), or vehicle control (PBS) for 1 hr before pulsing with 10 nM 17-estradiol for 30 min, in the continued presence of inhibitor, BDNF, or vehicle. Explants were then processed by Western immunoblot analysis for phosphoERK expression, using antibodies to phosphorylated p44/42 ^{MAP Kinase} (ERK1/2) (Thr202/Tyr204) (Cell Signaling Technology), as previously described (Singh et al., 1999, 2000).

In situ hybridization. Explants of the ERKO neocortex were processed for in situ hybridization, after 7 d in vitro, by a very sensitive, nonisotopic (digoxigenin) method, using a 48 base oligodeoxyribonucleotide (oligonucleotide) to an -specific sequence of the ER- LBD (BER2), as previously described (Miranda and Toran-Allerand, 1992). Briefly, the probe was 3'-end-labeled with digoxigenin-labeled deoxyuridine triphosphate (dUTP) by terminal deoxynucleotidyl transferase (TdT) (Invitrogen, Grand Island, NY). After hybridization of the synthetic oligonucleotide to the target cDNA, the hybrids were detected by enzyme-linked immunohistochemistry, using anti-digoxigenin antibodies (Fab fragment), conjugated to alkaline phosphatase (1:500; Boehringer Mannheim, Indianapolis, IN), and an enzyme-catalyzed blue-color reaction (5-bromo-4-chloro-3-indolyl phosphate and nitro-blue tetrazolium salt).

Immunocytochemistry. P7 ERKO and wild-type mice were anesthetized by hypothermia and killed painlessly by transcardial perfusion of saline, followed by 4% paraformaldehyde and 1% glutaraldehyde fixation. The neocortex was processed by pre- and post-embedding immunocytochemistry for ER- and flotillin, respectively. Sections (50 µm) were incubated in anti-ER- antibodies (C1355, 1:1000; or 6F11, 1:50), washed, and incubated in biotinylated horse-anti-rabbit or anti-mouse IgG, 1:250 (Vector Laboratories), incubated with avidin-biotin-peroxidase, 1:50 (Vector Laboratories), and followed by diaminobenzidine (DAB) (brown reaction product). Sections were then processed for electron microscopy, dehydrated, and flat embedded in Durcupan (EM Science, Gibbstown, NJ). Alternate ultrathin sections (Reichert-Jung Ultramicrotome) of the neocortex, immunolabeled for ER-, were further labeled for flotillin (1:50). Sections were washed and incubated in gold-conjugated (15 nm) goat anti-rabbit IgG (1:20) (EM Science), then washed and contrasted with saturated uranyl acetate. Ultrathin sections were examined using a Philips CM-10 electron microscope.

Estrogen binding assay. Duplicate aliquots of 1 mg each of protein lysate from ERKO P7 neocortex or wild-type adult uterus were precleared for 30 min, using anti-rabbit IgG-coated magnetic beads (Dynal AS). Precleared protein lysates were immunoprecipitated with anti-ER- antibodies [6F11 (Novocastra) or MC20 (Santa Cruz Biotechnology)] at 4°C overnight. Immunoprecipitated samples, Percoll-purified plasma membrane fractions, and Optiprep-purified CLM preparations (50 µg each) from P7 wild-type or ERKO neocortex were incubated with ³H-estradiol (2, 4, 6, 7, 16, 17-³H-estradiol, 100 Ci/mmol; NEN Life Sciences, Boston, MA) at 4°C for 18 hr. The incubation was terminated by adsorption of the binding sites onto an equal volume of hydroxylapatite (HAP) slurry in TESD buffer. HAP pellets were washed four times with Tris-buffered saline containing 0.2% Tween 20 buffer and extracted with 1 ml of absolute ethanol overnight at room temperature. The ethanol supernatants were transferred to liquid scintillation fluid (5 ml) and counted. Control tubes, used in assessing HAP adsorption of free steroid, contained HAP and the same buffer constituents, without addition of the membranes. Nonspecific binding was assayed in the membranes using the same amount of radioactive ligand plus 200-fold molar excess of unlabeled diethylstilbestrol (DES) (Sigma). Specific binding was calculated by subtracting nonspecific from total binding. The apparent affinity of the membrane binding sites was determined by incubation with a range of concentrations of ³H-estradiol (0.25-10 nM). The specificity of the binding sites was studied by coincubation of purified membranes with 2 nM ³H-estradiol in the presence of unlabeled progesterone, 17-estradiol, or 17-estradiol, added at either 25-fold or 500-fold molar excess.

Transient cerebral ischemia model. Details of the murine model of focal cerebral ischemia, using an intraluminal suture, have been described previously (Huang et al., 2000). Briefly, mice were anesthetized with 0.3 ml of intraperitoneal ketamine (10 mg/ml) and xylazine (0.5 mg/ml) and positioned supine on a rectal temperature-controlled operating surface (Yellow Springs Instruments, Yellow Springs, OH). Animal core temperature was maintained at 37 ± 2°C during surgery and for 90 min after surgery. A midline neck incision exposed the right carotid sheath under the operating microscope (Leica). The common carotid artery was isolated and the occipital, pterygopalatine, and external carotid arteries were each isolated, cauterized, and divided. Middle cerebral artery occlusion was accomplished by advancing a 13 mm heat-blunted 6-0 nylon suture via an arteriotomy made in the external carotid stump. After placement of the occluding suture, the external carotid artery was cauterized to prevent bleeding through the arteriotomy, and arterial flow was established. After 45 min the occluding suture was removed, and electrocautery was used to close the arteriotomy. The wound was closed with surgical staples. After 24 hr, the mice were anesthetized, decapitated, and brains were removed intact and placed in a mouse brain matrix (Activational Systems Inc, Warren, MI) for 1 mm sectioning. Sections were immersed in 2% triphenyltetrazolium chloride (Sigma) in 0.9% saline and incubated for 12 min at 37°C. Infarcted brain was identified as an area of unstained tissue. Slices containing tissue from the region surrounding the infarct (penumbra) and from the comparable region of the noninfarcted hemisphere were processed for immunoprecipitation and Western analysis, using 6F11 and MC20 antibodies to ER-, respectively. A total of 8 wild-type mice were studied.

	RESULTS

TOP ABSTRACT INTRODUCTION MATERIALS AND METHODS RESULTS DISCUSSION REFERENCES

P7 neocortex contains an ~62-63 kDa protein that is neither ER- nor ER- and is enriched in CLMs of the plasma membrane

Using antibodies directed against ER- and ER-, we found, by immunoprecipitation and Western immunoblot analysis of wild-type and ERKO P7 neocortical cell lysates, PNS, and CLM preparations, an hitherto unidentified protein, immunoreactive for the LBD of ER- but not ER-. We propose that this protein represents ER-X. Although immunoreactive for ER-, this protein has an apparent MW of ~62-63 kDa that is clearly different from that of ovarian ER- (67 kDa) and ER- (60 kDa) (Fitzpatrick et al., 1999) (Fig. 1a). Cell lysates and detergent-free, highly purified, CLM preparations (Smart et al., 1995) of both P7 neocortical wild-type and ERKO plasma membranes expressed this ~62-63 kDa protein (Fig. 1b). Although P7 wild-type neocortex expressed both the 67 kDa ER- band and the ~62-63 kDa ER-X band, P7 ERKO neocortex contained only the ~62-63 kDa band. P7 wild-type and ERKO neocortical CLM preparations were greatly enriched with the ~62-63 kDa protein (Fig. 1b). A striking reversal of the 67 kDa/~62-63 kDa ratio was seen in wild-type P7 neocortical CLM preparations, which, although highly enriched in the ~62-63 kDa form, were greatly diminished in the 67 kDa ER- band. The specificity and significance of the association of the ~62-63 kDa protein with CLMs was emphasized by the failure to detect immunoreactivity for other steroid receptors, such as ER- in CLM, non-CLM, and plasma membrane preparations (Fig. 1c), although its presence was clearly demonstrable in P7 neocortical cell lysates and in the nuclear fraction and PNS (Fig. 1c).

View larger version (49K): [in this window] [in a new window]   Figure 1.   ER-X is neither ER- nor ER-. a, Western immunoblots of P7 wild-type and ERKO neocortex and adult wild-type mouse ovary, using antibodies to the LBDs of ER- (Santa Cruz Biotechnology; MC-20; ovary and neocortex) and ER- (Zymed; ovary). The apparent MW of ER-X (~62-63 kDa) is clearly different from the MW of the mouse ER- (67 kDa) and ER- (60 kDa) ovarian controls. b, Whereas P7 wild-type neocortex contained both the 67 kDa ER- and the ~62-63 kDa ER-X bands, P7 ERKO tissues expressed only the ~62-63 kDa ER-X band. P7 wild-type and ERKO neocortical CLM preparations were greatly enriched with the ~62-63 kDa protein. A striking reversal of the ER-/ER-X ratio was seen in wild-type CLM preparations, in which the ~62-63 kDa form was highly enriched, whereas authentic 67 kDa ER- was considerably diminished. c, Absence of ER- from the plasma membrane, CLM, and non-CLM regions. Note the total absence of ER- from the wild-type plasma membrane and the CLM and non-CLM fractions. Note also the nuclear concentration of the 60 and 64 kDa isoforms of ER-. PM, Plasma membrane; non-CLM, non-caveolar-like membrane; CLM, caveolar-like membrane.

The purity of the CLM preparations was verified by demonstrating the presence of the CLM integral protein flotillin (Bickel et al., 1997) (Fig. 2a) and such CLM-enriched resident proteins as PKC- (Fig. 2b) and PKC- (data not shown) (Smart et al., 1995), and by the absence of the cytosolic protein paxillin (Fig. 2c), a cytoskeletal component associated with non-CLM regions of plasma membranes (Smart et al., 1995).

View larger version (42K): [in this window] [in a new window]   Figure 2.   Characterization and purity of the CLM preparations. a, Western immunoblots of CLMs show enrichment in flotillin, the neuron-specific, integral CLM protein. The purity of CLM preparations was verified by the presence of caveolar-enriched resident proteins such as PKC- (b), and by the absence of the cytosolic protein paxillin, a cytoskeletal component associated with non-CLM regions (c).

ER-X has an entirely different steroid specificity than either ER- or ER-

The steroid specificity for estrogen-induced activation of ERK1/2 phosphorylation is radically different from that of either ER- or ER-: ERK1/2 is not activated by either ER--selective ligands such as 16-iodo-17-estradiol (Singh et al., 2000) and PPT (Stauffer et al., 2000) (100 nM) (see Fig. 4b,c) or by ER--selective ligands such as genistein and coumestrol (Singh et al., 2000) but is activated equally well by picomolar concentrations of 17- and 17-estradiol (Fig. 3a,b). In wild-type cultures 17-estradiol, a natural stereoisomer of 17-estradiol that is generally considered to be transcriptionally inactive, elicited a stronger, sustained activation of ERK1/2 at the 1-10 pM (10¹² M) range (Fig. 3b) than did 17-estradiol (0.1-10 nM) (Fig. 3a). What makes this response so astonishing is that 17-estradiol, which, like 17-estradiol, is derived from aromatization of androgens, but whose site of synthesis is unclear, has a 100-fold lower affinity for ER- than 17-estradiol (Hajek et al., 1997). Significantly, higher levels of 17-estradiol were required for ERK activation in wild-type neocortical cultures (Fig. 3a), perhaps reflecting the need to overcome the inhibitory effect of ER- on ERK1/2 phosphorylation (Singh et al., 2000) (Fig. 4), which, unlike 17-estradiol, 17-estradiol activates as well. That the inhibitory presence of ER- influences dose responsiveness is suggested by observation that in the ER--deficient ERKO neocortical explants, 17-estradiol, like 17-estradiol, is also able to elicit activation of ERK in the 1-10 pM range (data not shown).

View larger version (43K): [in this window] [in a new window]   Figure 3.   ER-X is exquisitely sensitive to picomolar concentrations of 17- and 17-estradiol. Western immunoblot of ERK1/2 phosphorylation elicited in wild-type neocortical explants by 17-estradiol (a) and 17-estradiol (b). Bottom blots, Reprobing with antibodies to total nonphosphorylated ERK1/2 to verify equal loading of ERK1/2 protein across lanes. pERK, phosphoERK. Densitometry confirmed equal loading. Note that significantly higher levels of 17-estradiol were required for ERK activation, perhaps reflecting the need in wild-type cultures to overcome the inhibitory effect of ER- on ERK phosphorylation, which, unlike 17-estradiol, 17-estradiol activates as well.

View larger version (36K): [in this window] [in a new window]   Figure 4.   Estrogen-induced activation of ERK1/2 in CLMs and PNS. Western immunoblots: a, exposure of highly purified, P7 ERKO neocortical CLMs to 17-estradiol (0.1 nM) and 17-estradiol (10 nM) for 30 min elicited MEK-dependent (U0126) phosphorylation of ERK1 and ERK2. Non-CLM regions were unresponsive. Densitometry confirmed equal loading of protein. b, Exposure of P7 wild-type neocortical PNS to 17-estradiol (0.1 nM) and 17-estradiol (10 nM) for 10 min, 37°C elicited MEK-dependent (U0126) phosphorylation of ERK1 and ERK2. Note that, not only did the ER--selective ligand PPT reduce ERK phosphorylation levels below baseline (0) very significantly, but that the level of ERK1/2 phosphorylation, elicited by 17-estradiol, was also significantly lower than after exposure to 17-estradiol. This difference may be attributed to the fact that P7 wild-type neocortex is also enriched in ER- which, because it is activated by 17- (but not 17-) estradiol and exerts its inhibitory effect on ERK1/2, as was also seen after exposure to PPT. Bottom blots, Reprobing with antibodies to nonphosphorylated ERK1/2 to verify equal loading of ERK protein across lanes. Densitometry confirmed equal loading. c, Densitometric analysis of ERK activation in wild-type PNS shown in b. These findings confirm that ER- is a strong inhibitor of ERK activation, a measure of which is shown by the ability of PPT to effectively prevent ERK activation even in the face of the strong activation of ERK elicited by the PPT vehicle ethanol.

Estrogen elicits ERK1/2 activation in CLMs

To provide direct evidence that the CLM-associated ~62-63 kDa ER-X protein is connected with estrogen-induced ERK1/2 activation, we showed that exposure of highly purified, P7 ERKO neocortical CLMs to 17-estradiol (10 nM) and 17-estradiol (0.1 nM) for 30 min elicited phosphorylation of ERK1/2 (Fig. 4a). In both instances ERK activation was inhibited by the MEK inhibitor U0126 (Fig. 4a). In contrast, non-CLM regions of the plasma membrane, exposed similarly, did not respond (Fig. 4a).

ER- is an inhibitory regulator of ERK1/2 activation in PNS

We then investigated in wild-type PNS, a cell-free system, whether ER- is an inhibitory regulator of estrogen-induced ERK1/2 activation, as we had shown previously in neocortical explants (Singh et al., 2000). Using the ER--selective ligand PPT (Stauffer et al., 2000) (100 nM) in wild-type neocortical PNS, we found that MEK-inducible ERK1/2 phosphorylation was dramatically reduced below baseline (Fig. 4b). Of particular note, furthermore, were the findings that the levels of 17-estradiol-induced ERK1/2 phosphorylation were significantly less than after exposure to 17-estradiol, although both were inhibited by the MEK inhibitor U0126. This difference in responsiveness may be attributed to the fact that, at P7, wild-type neocortex is also enriched with maximal levels of ER- (Gerlach et al., 1983) which, when activated by 17- (but not 17-) estradiol, is able to exert its inhibitory effect on ERK1/2, as is also seen after exposure to PPT. These findings confirm that ER- is a strong inhibitor of ERK1/2 activation, a measure of which is given by the ability of PPT to effectively prevent activation of ERK1/2 even in the face of strong ERK1/2 activation, elicited by the PPT vehicle ethanol (Fig. 4b,c). These findings provide not only additional proof that ER- does not mediate activation of the MAPK cascade but also compelling evidence confirming the role of ER- as an inhibitory modulator of ERK1/2 activation.

Cholesterol disruption in CLMs decreases estrogen activation of ERK

CLMs, like caveolae, are highly enriched in cholesterol, glycosphingolipids, sphingomyelin, and lipid-anchored membrane proteins, which serve as multivalent scaffolding onto which many signaling kinases assemble to generate preassembled signaling complexes. Eighty to ninety percent of plasma membrane cholesterol is concentrated within caveolae/CLMs, where it plays a critical role in maintaining receptor protein association within the CLM domain (Rothberg et al., 1990). The sterol-binding agent Nystatin has been used extensively to document the association of growth factor receptors with caveolae/CLMs (Huang et al., 1999). To determine whether selective disruption of cholesterol in CLMs impairs the ability of estrogen to elicit ERK1/2 phosphorylation, we exposed P9 neocortical explants to Nystatin (50 µg/ml) for 1 hr before pulsing with 17-estradiol (10 nM), BDNF (100 ng/ml), or the vehicle control (PBS) for 30 min (Fig. 5) and then processing for ERK1/2 phosphorylation by Western blot analysis. We found that disruption of membrane cholesterol decreased the ability of both estradiol and BDNF to elicit ERK1/2 phosphorylation, providing additional evidence of the contributions of CLMs to estradiol-induced ERK1/2 activation.

View larger version (75K): [in this window] [in a new window]   Figure 5.   Disruption of cholesterol in CLMs impairs ERK activation. Selective disruption of membrane cholesterol by Nystatin in 9-d-old wild-type neocortical explants decreased the ability of estradiol and the BDNF control to elicit ERK phosphorylation. Bottom blots, Reprobing with antibodies to nonphosphorylated ERK1/2 to verify equal loading of ERK protein across lanes. Densitometry confirmed equal loading.

ER-X has homology with ER- LBD and is expressed in the plasma membrane

Using an oligonucleotide probe directed against an -specific region of the ER- LBD (BER2) (Miranda and Toran-Allerand, 1992), we found widespread distribution of the blue ER--like hybridization signal in neurons of cultured slices of the ER--deficient P2 ERKO neocortex, 17 d in vitro (Fig. 6). This pattern of hybridization in ERKO neocortex suggests that, in view of the absence of ER-, the oligonucleotide sequence used may share some homology with ER-X mRNA.

View larger version (90K): [in this window] [in a new window]   Figure 6.   ER-X has homology with the LBD of ER-. Whole-mount of a P2 ERKO neocortical explant, 17 d in vitro. The culture was stained for ER- mRNA by in situ hybridization with a 48 base oligonucleotide probe to an -specific region of the ER- LBD (BER2; Miranda and Toran-Allerand, 1992) and shows the ER--like mRNA hybridization signal in neocortical neurons. Residual, untranslated ER- mRNA? A splice variant of ER- mRNA? Or the mRNA for a novel ER, ER-X?

Direct evidence that ER-X may be a neuronal plasma membrane-associated ER protein with some homology to ER- was also obtained in the ERKO neocortex by means of light and electron microscopic immunohistochemistry (Fig. 7a-e). Using polyclonal antibodies, generated against the final 14 C-terminal amino acids of the rat ER and highly specific for ER- (C1355, Upstate Biotechnology) (Schreihofer et al., 1999), large numbers of immature ERKO neocortical neurons with unstained nuclei were seen (Fig. 7a,b). Immunoreactivity was clearly localized to the cell membrane and cytoplasm and not in the nucleus. In Figure 7b, a blood vessel (V) is in close proximity to a labeled dendrite, an association which suggests a mechanism by which estrogen could get even more efficiently onto ER-X. On the other hand, using monoclonal antibodies generated against full-length mouse ER- (6F11; Novocastra) (Fig. 7D,E) and said to recognize the 5' N terminus region, the opposite result was obtained: nuclear labeling was observed but no cytoplasmic or membrane labeling was seen. Because we have found that 6F11 cross-reacts significantly with ER- by Western blotting (data not shown), the nuclear labeling observed here most likely reflects intranuclear ER-, which is normally expressed in both wild-type and ERKO neocortex. Association of the ~62-63 kDa protein with CLMs was further documented at the ultrastructural level on ultrathin cryostat sections of P7 ERKO neocortex by demonstrating immunoreactive flotillin, labeled by gold particles, colocalized with horseradish peroxidase-labeled immunoreactivity for ER- on a neocortical dendritic spine (Fig. 7C).

View larger version (132K): [in this window] [in a new window]   Figure 7.   Direct evidence in ERKO that ER-X is a neuronal plasma membrane-associated receptor with some homology to the ER- LBD. A, Using antibodies highly specific for an -specific region of the LBD of ER- (C1355), large numbers of immature immunoreactive neocortical ERKO neurons with unstained nuclei are seen. B, The immunoreactivity is clearly localized to the cell membrane and cytoplasm and not in the nucleus. D, E, Antibodies, raised against the full-length ER- molecule, said to recognize epitopes in the 5', N-terminal region (6F11), but which we have found also to cross-react significantly with ER-, show widespread nuclear labeling with no cytoplasmic or membrane labeling seen. The nuclear labeling observed most likely reflects intranuclear ER-, which is normally expressed in both wild-type and ERKO neocortical neurons. C, CLM association of ER-X in ERKO neocortical neurons was further documented at the ultrastructural level by demonstrating immunoreactive flotillin (gold particles), colocalized with immunoreactivity for the ER- LBD (horseradish peroxidase) on a mushroom-like neocortical dendritic spine. Scale bars, 10 µm.

ERKO neocortical plasma membranes contain an estrogen-binding protein (ER-X)

We determined that neocortical plasma membranes contain a unique estrogen-binding protein by scintillation counting of ³H-estradiol binding to the ~62-63 kDa ER-X protein in highly purified P7 ERKO CLM preparations. In these preparations, the only detectable ER-immunoreactive material present was the ~62-63 kDa protein (Fig. 1). Binding of 10 nM ³H-estradiol to P7 ERKO CLMs appeared to be specific and saturable, in that it was suppressed in the presence of unlabeled diethylstilbestrol (DES). Neocortical CLM preparations from P7 ERKO mice, shown to be highly enriched in ER-X, were similarly highly enriched in DES-sensitive estrogen binding (282.12 fmol/mg CLM protein), as compared with P7 ERKO neocortical lysates (9.94 fmol/mg lysate protein) and wild-type adult uterine lysates (38.85 fmol/mg lysate protein). Further characterization of the membrane binding sites was achieved using Percoll-fractionated plasma membranes, containing both CLM and non-CLM components, to increase the yield of total membrane sufficiently to allow construction of binding isotherms and performance of specificity studies. In Percoll-purified membranes from P7 ERKO neocortices, as in CLMs, the only detectable ER-immunoreactive protein present was the ~62-63 kDa band (data not shown). Membranes from both P7 ERKO and P7 wild type neocortex contained a high-affinity, saturable ³H-estradiol binding site (K_d, ~1.6 nM) (Fig. 8A). Addition of 50 nM unlabelled 17-estradiol or 17-estradiol markedly inhibited binding of ³H-estradiol. In the presence of a 1 µM concentration of either estrogen, binding of the tritiated ligand was reduced to the nonspecific levels observed in the presence of excess DES (Fig. 8B). Unlabelled progesterone, by contrast, was less effective than either estrogen, progesterone only partially suppressing binding of ³H-estradiol when added in 500-fold molar excess (Fig. 8B).

View larger version (24K): [in this window] [in a new window]   Figure 8.   Binding of 3H-estradiol to Percoll-purified plasma membranes from P7 ERKO and wild-type mouse neocortex. A, Identical amounts of membrane protein (50 µg/tube) were incubated with varying concentrations of 3H-estradiol (0.3-8 nM) for 18 hr at 4C. The reaction was terminated by addition of hydroxylapatite (HAP). The membranes and HAP were sedimented by centrifugation in a microfuge, and the pellet was washed four times to remove free steroid. Radioactivity in the pellets was extracted with ethanol and counted. Nonsaturable binding, assessed in the presence of 1 µM unlabeled DES, was subtracted from the total counts, and the saturable binding was plotted as the ratio of bound-unbound ligand versus the concentration of bound 3H-estradiol. Similar concentrations of high-affinity binding (equilibrium dissociation constant, Kd, ~1.6 nM) were observed in wild-type and ERKO membranes. B, Specificity of the binding site in Percoll-purified membranes from P7 ERKO mouse neocortex. Aliquots of plasma membrane were incubated with 2 nM 3H-estradiol for 18 hr at 4°C in the presence and absence of different concentrations (50 nM and 1 µM) of 17-estradiol, 17-estradiol, or progesterone. Bound 3H-estradiol was separated by sedimentation with HAP and counted at an efficiency of 50%. Data represent the number of bound counts (after subtraction of HAP-only blank control tubes, containing no membrane protein) expressed as the means ± SD of triplicate determinations. The horizontal dashed line indicates the level of nonspecific binding observed in the presence of 1 µM DES.

ER-X is developmentally regulated in the brain and uterus

Expression of the ~62-63 kDa ER-X protein is developmentally regulated and is maximally expressed ~P7-P10 in both the neocortex and uterus (Fig. 9a,b). During the first postnatal month, wild-type and ERKO neocortical and uterine levels of the ~62-63 kDa protein declined until P21 and became dramatically reduced in the adult, which expressed little of this protein.

View larger version (57K): [in this window] [in a new window]   Figure 9.   ER-X is developmentally regulated. ER-X expression is developmentally regulated and is maximally expressed at ~P7-P10 in the neocortex (a) and the uterus (b). During the first postnatal month, wild-type and ERKO neocortical ER-X levels decline dramatically and become barely visible in the adult.

ER-X is upregulated in a rodent model of brain injury

To test whether re-expression of the developmentally regulated ER-X might return after brain injury in the adult, as has been reported for the developmentally regulated ER- (Dubal et al., 2001), we analyzed a mouse ischemic stroke model, elicited by transient intraluminal middle cerebral artery occlusion (Huang et al., 2000). Tissue from the region surrounding the infarct (the penumbra) was compared with the comparable region of the noninfarcted neocortex of the opposite side, 24 hr after occlusion. Using immunoprecipitation, followed by Western blotting, we found upregulation of the ~62-63 kDa protein in the penumbra (Fig. 10) to levels comparable with those present during development, as compared with the noninfarcted side that remained unchanged. There was also upregulation of ER- (Fig. 10), as has been shown previously (Dubal et al., 2001).

View larger version (49K): [in this window] [in a new window]   Figure 10.   ER-X is upregulated after ischemic brain injury in the adult. Comparison of ER- and ER-X expression in the infarcted and noninfarcted adult neocortex. After a large ischemic infarct in the neocortex produced by middle cerebral artery occlusion, there was not only upregulation of ER- expression in the penumbra of the ligated, ischemic side but also upregulation of ER-X therein as well, suggesting re-expression of a developmental mechanism normally latent in the adult. Note the lack of significant ER-X expression on the noninfarcted side. MCF-7 mammary tumor cells and adult uterus = ER- controls; P7 neocortex = ER-X control.

	DISCUSSION

TOP ABSTRACT INTRODUCTION MATERIALS AND METHODS RESULTS DISCUSSION REFERENCES

These data point strongly to the existence of a novel, plasma membrane-associated, putative estrogen receptor (ER-X). Although membrane ERs have been identified immunologically as ER- in several cell and tissue systems (Blaustein, 1992; Watson et al., 1999; Razandi et al., 1999; Milner et al., 2001), our findings suggest that ER-X is a unique, functionally distinct, and hitherto unidentified receptor, based on its MW, ligand specificity, cellular localization, and apparent response characteristics. Although ER-X reacts with antibodies to the ER- LBD, ER-X is not membrane-associated ER-. Its apparent MW of ~62-63 kDa is clearly different from that of both ER- (67 kDa) and ER- (60 kDa) (Fig. 1a). Although a functional isoform of ER- with an additional 18 amino acids inserted in the LBD has been identified in rat and mouse tissues (ER-2) (Petersen et al., 1998), ER-X cannot represent ER-2, because (1) antibodies directed against the ER- LBD cross-react with ER-X and do not recognize intranuclear ER-. (2) The anti-ER- antibody we used (Zymed) does not react with ER- but does cross-react on Western blots with the molecular isoforms of rat ER- observed in tissue lysates (N. J. MacLusky and I. S. Nethrapalli, unpublished observations): no immunoreactivity was detected with this antibody in blots from CLMs enriched in ER-X. (3) ERK1/2 is not activated by ER- or ER--selective agonists (Singh et al., 2000) (Fig. 4b). (4) Unlike ER- or ER-, ER-X is not stereospecific, responding equally well to picomolar concentrations of 17- and 17-estradiol (Fig. 3a,b), whereas ER- and ER- exhibit a markedly higher affinity for 17- than for 17-estradiol (Kuiper et al., 1997).

ER-X is part of a multimolecular CLM complex, comprising immunoreactivity for ER- (but not ER-) in association with hsp90, members of the MAPK cascade (Toran-Allerand et al., 1999; Singh et al., 1999; Toran-Allerand, 2000) and flotillin, the multivalent, 48 kDa scaffolding protein and neuronal homolog of the caveolar protein caveolin (Bickel et al., 1997). Two recent studies (Levin, 2002; Razandi et al., 2002), published after this paper was submitted, reported association of ER- immunoreactivity with caveolae in vascular and breast cancer (MCF-7) cells. Although caveolin-associated ER was identified by the authors as ER- (Razandi et al., 2002), the MW of the immunoreactive band was stated to be 62 kDa, not 67 kDa, as would be expected for authentic full-length ER-. Rather than demonstrating caveolar association of ER-, as Razandi et al. (2002) concluded, these data are consistent with the observations presented here. In vascular and MCF-7 cells, like neuronal CLMs, caveolar-associated ER- immunoreactivity represents primarily a protein with an apparent MW ~5 kDa less than that of authentic ER-. In brain, both P7 wild-type and ERKO neocortical CLM preparations were greatly enriched with the immunoreactive ~62-63 kDa ER-X protein (Fig. 1b) and depleted of ER- and ER- (Fig. 1b,c), supporting the selectivity and specificity of the ER-X association with CLMs.

Surprisingly, in both wild-type and ERKO neocortical explants and CLMs, 17-estradiol, the natural stereoisomer of 17-estradiol with 100-fold lower affinity for ER-, (Hajek et al., 1997) also elicited sustained MEK-dependent activation of ERK1/2 in the picomolar range (Figs. 3b, 4a,b). We showed earlier that ER-- and ER--selective ligands failed to elicit ERK1/2 activation in wild-type neocortical explants (Singh et al., 2000) and suggested that ER- may be an inhibitory regulator of ERK activation. This has been confirmed in the PNS cell-free system (Fig. 4b,c). The absence of an inhibitory response in ERKO PNS (data not shown) is consistent with the absence of authentic 67 kDa ER- from ERKO brains.

Nystatin disrupts cholesterol in cell membranes (Iwabuchi et al., 2000) by forming globular deposits that alter the planar organization of the membrane (McGookey et al., 1983), thereby selectively inhibiting caveolar trafficking without altering other cell functions such clathrin-mediated endocytosis (Ros-Baro et al., 2001) or intracellular receptor trafficking back to the cell surface (Subtil et al., 1999). Nystatin (50 µg/ml) has been shown to significantly reduce cellular cholesterol content without appreciably affecting cell viability. This concentration of Nystatin impaired estradiol induced ERK1/2 activation (Fig. 5).

The existence of plasma membrane-associated ERs (Pietras and Szego, 1977) has been controversial because of previous failures to isolate and characterize such a membrane-associated receptor. Hypothetical mechanisms have included plasma membrane versions of classical intranuclear ER- and ER- (Blaustein, 1992; Watson et al., 1999; Razandi et al., 1999; Milner et al., 2001), novel members of the ER family (Das et al., 1997; Gu et al., 1999; Nadal et al., 2000), G-protein-coupled receptors (Kelly and Wagner, 1999; Filardo et al., 2000; Wyckoff et al., 2001), or even growth factor-like receptor tyrosine kinases (Anuradha et al., 1994).

That ER-X may have sequence homology with the ER- LBD was suggested by (1) the strong hybridization signal obtained in ERKO neocortical explants with an oligonucleotide probe specific for the ER- LBD (Miranda and Toran-Allerand, 1992) (Fig. 6) and (2) ER--like immunoreactivity in ERKO neocortex, using antibodies to the ER- LBD (Fig. 7A,B) but not with those recognizing the N-terminal region (Fig. 7D,E). To generate ERKO, the ER- gene was disrupted by insertion of a 1.8 kb PGK-Neomycin sequence in the region of exon 2, ~280 bp downstream of the transcription start codon (N terminus) (Lubahn et al., 1993), a region far upstream from the LBD (exons 4-8). Therefore, ER--like mRNA found in ERKO neocortex may represent either (1) residual, untranslated ER- mRNA, (2) a splice variant of ER-, or (3) ER-X mRNA itself. Residual, weak estrogen binding not attributable to ER- has been reported in both ER- (ERKO) and ER-/ER-- (double) knock-out adult mouse brains (Shughrue et al., 2002). This binding was identified in ERKO only as a splice variant of ER- at exon 2 that may regulate the progesterone receptor. Nonetheless, there are compelling reasons to believe that ER-X does not represent the protein product of such a splice variant. A splice variant at exon 2 would contain exactly the same LBD sequence as authentic ER-. However, the ligand specificity of ER-X is clearly different from that of ER- in that ER-X responds equally well to picomolar concentrations of 17- and 17-estradiol (Fig. 3a,b). Finally, ER-X simply cannot represent expression of a protein derived from the targeted gene disruption, used to generate ERKO mice, because ER-X is present at comparable levels in P7 wild-type and ERKO neocortex (Fig. 1b). Earlier studies of cellular variations in ER mRNA translation (Toran-Allerand et al., 1992) have provided data consistent with the hypothesis that some of the ER--like mRNA detected by in situ hybridization may actually represent ER-X mRNA. Although estrogen binding and ER mRNA expression always colocalized, neurons expressing ER mRNA did not always exhibit nuclear binding, and there was no clear-cut relationship between the widespread hybridization signal (Miranda and Toran-Allerand, 1992) and the limited extent of estrogen binding (Gerlach et al., 1983); evidence of ER-X mRNA?

Our data do not prove that the ~62-63 kDa ER--immunoreactive protein binds estrogen. The SDS-PAGE conditions required to separate the ~62-63 kDa protein are incompatible with retention of binding site integrity. Neve