The Journal of Neuroscience, May 15, 2003, ():
Multiple Cell Populations in the Early Postnatal Subventricular Zone Take Distinct Migratory Pathways: A Dynamic Study of Glial and Neuronal Progenitor Migration
J. Neurosci.
Suzuki and Goldman 23 (10): 4240.
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Files in this Data Supplement:
- Supplemental Movie 1
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In the SVZ, GFP-positive cells are oriented perpendicular to the vimentin-positive processes. This movie shows rotation of Fig 7A (See Text) along the Z-axis.
- Supplemental Movie 2
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Time-lapse videoimaging of GFP-labeled cells in living slices. Lateral sagittal slice at the striatal level after a middle injection. The level of the slice corresponds to the sagittal section shown in Fig 3A and B. The slice was imaged from 20 hours post injection (hpi) to 44 hpi. GFP-labeled cells residing in the SVZ as well as migrating radially into the overlying white matter and cortex in the vicinity of the injection site are shown. Tracking lines of representative cell movements are shown in different colors; Orange lines: Cells migrating within the SVZ. They largely migrate rostro-caudally, however, some change direction. Red lines: Cells radially migrating out of the SVZ into the white matter. None of them migrates in a rostro-caudal direction within the SVZ before their emigration into the white matter. Cells also show radial, straight migration in the white matter. One of the tracked cells shows a ventrally-oriented movement. Blue lines: Cells showing movements off the radial path. Bar=200mm.
- Supplemental Movie 3
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Horizontal slice at the level of the striatum 3 days after a caudal injection. The level corresponds to that of Fig 5. The slice was imaged 3 days post injection for 4 hr 24 min. Labeled cells are largely confined to the SVZ but a few migrate in the striatum only near the injection level. Tracking lines of representative cell movements are shown in different colors; Orange lines: Cells within the SVZ showing straight rostro-caudal migration. Green: A cell turning around within the SVZ. Blue lines: Cells turning at the SVZ border with the striatum. Pink line: A cell migrating in the striatum. Bar=200mm.
- Supplemental Movie 4
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A medial sagittal slice 3 days after a middle injection showing the anterior part of the SVZ and the vertical limb of the RMS (rostral to left). Labeled cells have migrated from the injection site (not appearing in this imaging field) towards the RMS. The slice was imaged for 5 hr 33 min. Tracking lines of representative cell movements are shown in different colors; Red lines: Cells migrating along the dorso-caudal to rostro-ventral bend formed by the SVZ and the vertical limb of the RMS. Cells show bidirectional movements. Blue lines: Cells changing directions in the middle of the SVZ. Yellow lines: Cells turning at the SVZ border with the white matter. Bar=200mm.
- Supplemental Movie 5
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Cell migration in the anterior SVZ continuing into the RMS in a medial sagittal slice 3 days after middle injection (rostral to left). The slice was imaged for 4 hr 3 min. Tracking lines of representative cell movements are shown in different colors; Blue and pink lines: Tangential movements. Some cells are migrating in a distal to proximal direction (pink lines). Yellow lines: A cell turning at the SVZ-white matter border. V: lateral ventricle, WM: white matter, Ctx: cortex, Str: striatum Sep Nuc: septal nuclei. Bar=200mm.
