The Journal of Neuroscience, July 2, 2003, ():
Four-Dimensional Migratory Coordinates of GABAergic Interneurons in the Developing Mouse Cortex
J. Neurosci.
Ang et al. 23 (13): 5805.
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These movies of 2-photon time series experiments are best viewed with QuickTime Player V.5.0 or higher. Since each clip contains many cells and their associated movements, download the clip to your hard drive and set the movie to loop (under “Movie” on the taskbar of the application, or CTRL+L). For viewers with small or low resolution monitors, some of the larger movies will slow system performance. In this case, we recommend that the movie player be re-sized so that it is smaller.
Files in this Data Supplement:
- Movie 1: Tangential Migration in the Marginal Zone (coronal)
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Duration: 2 hrs
Frame Interval: 2 min
Stain: CellTracker Green
Preparation: E15 Coronal Slice
10 _m stack projection
In this coronal view of the cortical plate, two migrating neurons can be seen. The first neuron emerges obliquely to the left and uses its leading process (asterisk) to detect the surface of the MZ at which point it stops its migration. The second neuron travels tangentially from right to left in the marginal zone (train of white arrowheads). This coronal view belies the multi-directional movements possible in interneurons migrating on the neocortical surface (see movie 2). The movie selection is repeated three times.
- Movie 2: Tangential Migration in the Marginal Zone (en face)
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Duration: 14.5 hrs
Frame Interval: 25 min
Stain: CellTracker Green
Preparation: E15 Neocortical Flap
24 _m stack projection
In this tangential view of the neocortical surface, many tangentially oriented neurons are seen migrating orthogonally through the marginal zone during the local positioning phase of their migration. The movement is smooth and accomplished by leading process extension as well as somal translocation. Several cells descend into the underlying cortical plate (below the level of analysis) and disappear from view. The movie selection is repeated three times.
- Movie 3: Fast Caudal Migration Sampled from Stream III
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Duration: 4.1 hrs
Frame Interval: 20 min
Stain: CellTracker Green
Preparation: E15 Whole Brain
20 _m stack projection
In this movie, a “bird’s-eye” tangential view of an E15 wholebrain preparation, MZ cells are found moving rapidly through the first phase of their migration within Stream III (see Fig. 1). Stream III projects from the dorsal olfactory bulb caudally along the lateral surface of the telencephalic vesicle. Thus, these migrating cells, whose leading processes are not well stained most likely because they are moving so quickly, all move caudally (from left to right in the movie). The movie selection is repeated three times.
- Movie 4: Tangential Migration and Integration into the Neocortical Plate
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Duration: 9.7 hrs
Frame Interval: 5 min
Stain: CellTracker Green
Preparation: E14 Coronal Slice
20 _m stack projection
In the middle of the frame of this coronal slice, two cells are seen at different phases of their migration (see also Figure 5A). An initial cell, which had previously migrated tangentially (not shown), moves downward into the neocortical plate. A second cell, which appears in the marginal zone just above the first cell, moves tangentially to the right following its long leading process. The movie selection is repeated twice.
- Movie 5: Descent into the Neocortical Plate
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Duration: 16 hrs
Frame Interval: 30 min
Stain: CellTracker Green
Preparation: E15 Neocortical Flap
30 _m stack projection
Three cells (red arrows) in this tangential view dive deep into the cortical plate in a local sector of the dorsal neocortical wall (see also Figure 5B). Few cells integrate into the same position of the cortical plate, but local sectors appear to receive multiple cells within a relatively short time window. The movie selection is repeated three times.
- Movie 6: Searching the Neocortical Plate
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Duration: 9 hrs
Frame Interval: 10 min
Stain: CellTracker Green
Preparation: E14 Coronal Slice
10 _m stack projection
A tangentially migrating cell in the MZ (red arrow) uses its long leading process to search the underlying cortical plate (see also Figure 6). Despite its extensive searching, it continues to migrate tangentially. Contrast this with the downward migrating cell to the left (red arrow; second loop of the movie). This cell enters and positions itself within the layers of the underlying cortical plate. The movie selection is repeated twice.
