The Journal of Neuroscience, July 16, 2003, ():
Dual Modes of Endoplasmic Reticulum-to-Golgi Transport in Dendrites Revealed by Live-Cell Imaging
J. Neurosci.
Horton and Ehlers 23 (15): 6188.
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Files in this Data Supplement:
- Supplementary Movie 1a
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VSVG--YFP proceeds through the early secretory pathway in neuronal dendrites. Stacks of confocal images were acquired every 7.5 sec for a total elapsed time of 1:08 (minutes:seconds). The frames are the maximum point projections of confocal stacks. The image is 10 ƒÝm wide. A, Time lapse of the dendrite of a neuron expressing VSVG--YFP (15 DIV) immediately after release from the 39.5¢XC block. The movie shows the interval from 0:00 to 1:08. B, Same dendrite as above, but imaged 5 min after release from the 39.5¢XC block to show that VSVG--YFP accumulated at relatively stationary puncta within the dendrites. The arrowhead points to one of these puncta. Not all of the structures are stationary, because VSVG--YFP is beginning to bud from these sites into tubulovesicular carriers. This movie shows the interval from 5:00 to 6:08. C, The same dendrite was imaged 15 min after release from the 39.5¢XC block to show the VSVG--YFP trafficking in highly mobile tubulovesicular carriers. The arrowheads point to mobile carriers This movie represents the interval from 15:00 to 16:08.
- Supplementary Movie 1b
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VSVG--YFP proceeds through the early secretory pathway in neuronal dendrites. Stacks of confocal images were acquired every 7.5 sec for a total elapsed time of 1:08 (minutes:seconds). The frames are the maximum point projections of confocal stacks. The image is 10 ƒÝm wide. A, Time lapse of the dendrite of a neuron expressing VSVG--YFP (15 DIV) immediately after release from the 39.5¢XC block. The movie shows the interval from 0:00 to 1:08. B, Same dendrite as above, but imaged 5 min after release from the 39.5¢XC block to show that VSVG--YFP accumulated at relatively stationary puncta within the dendrites. The arrowhead points to one of these puncta. Not all of the structures are stationary, because VSVG--YFP is beginning to bud from these sites into tubulovesicular carriers. This movie shows the interval from 5:00 to 6:08. C, The same dendrite was imaged 15 min after release from the 39.5¢XC block to show the VSVG--YFP trafficking in highly mobile tubulovesicular carriers. The arrowheads point to mobile carriers This movie represents the interval from 15:00 to 16:08.
- Supplementary Movie 1c
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VSVG--YFP proceeds through the early secretory pathway in neuronal dendrites. Stacks of confocal images were acquired every 7.5 sec for a total elapsed time of 1:08 (minutes:seconds). The frames are the maximum point projections of confocal stacks. The image is 10 ƒÝm wide. A, Time lapse of the dendrite of a neuron expressing VSVG--YFP (15 DIV) immediately after release from the 39.5¢XC block. The movie shows the interval from 0:00 to 1:08. B, Same dendrite as above, but imaged 5 min after release from the 39.5¢XC block to show that VSVG--YFP accumulated at relatively stationary puncta within the dendrites. The arrowhead points to one of these puncta. Not all of the structures are stationary, because VSVG--YFP is beginning to bud from these sites into tubulovesicular carriers. This movie shows the interval from 5:00 to 6:08. C, The same dendrite was imaged 15 min after release from the 39.5¢XC block to show the VSVG--YFP trafficking in highly mobile tubulovesicular carriers. The arrowheads point to mobile carriers This movie represents the interval from 15:00 to 16:08.
- Supplementary Movie 2
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VSVG--GFP traffics in highly mobile carriers that move bidirectionally throughout the dendrites. Time lapse of a neuron (21 DIV) expressing VSVG--GFP is shown, beginning 5 min after release from the 39.5¢XC block. Stacks of confocal images were acquired 1 per 3.9 sec for a total elapsed time of 6:29. The movie shows the interval from 5:00 to 11:29 after release from the ER. The frames are maximum point projections of confocal stacks. The image is 40 ƒÝm wide.
- Supplementary Movie 3
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Mobile post-ER carriers merge with more stable, stationary structures. Time lapse of VSVG--GFP trafficking via a tubulovesicular carrier along the dendrite of an expressing neuron is shown. The carrier (arrowhead) traffics along a curvilinear path before merging with the stationary structure marked by the arrow. The frames are maximum point projections of confocal stacks. The stacks of confocal images were acquired every 7.7 sec. The movie shows the interval from 9:45 to 13:47 after the release from the ER. The image is 10 ƒÝm wide.
- Supplementary Movie 4
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ER exit sites are stable and stationary domains within neuronal dendrites. Sec24-YFP labels ER exit sites along the dendrite of an expressing neuron (15 DIV). The time lapse of the frames was acquired every 3.8 sec for 1:40 min. Frames are maximum point projections of confocal stacks. The image is 2.5 ƒÝm wide.
- Supplementary Movie 5
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Dendritic ER exit sites function in secretory trafficking. VSVG--CFP buds from an ER exit site. Some of the cargo buds into a carrier that traffics to the left (arrow), whereas the remaining VSVG--CFP is concentrated at the stationary ER exit site (arrowhead). The frames are maximum point projections of confocal stacks. The stacks were acquired every 8 sec. The image is 5 ƒÝm wide.
- Supplementary Movie 6
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Cargo carriers fuse with dendritic Golgi outposts. A mobile post-ER carrier (arrow) fuses with a stationary dendritic Golgi outpost (arrowhead). The frames are maximum point projections of confocal stacks. The stacks were acquired every 7.5 sec from 15:00 to 16:08 (minutes:seconds) after release from the 39.5¢XC block. The image is 4 ƒÝm wide.
