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Figure 1. A, Representative example of the ventrotemporal quadrant of a
whole-mount lesioned retina in which retinal fibers were retrogradely labeled
with DiI. The portion of the retina containing axotomized RGCs is clearly
visible (bottom right). An arrow points to the optic disk. V, Ventral; T,
temporal. Scale bar, 750 µm. B, Neurolucida drawing of a
flat-mount lesioned retina. The extent of the retinal damage is shown in gray.
V, Ventral; T, temporal. C, Confocal image of a coronal section
through the colliculus of a lesioned animal that was intravitreally injected
with Alexa 594-conjugated CTB. The collicular scotoma is visible as the
unstained area on the medial part of the superficial gray (left). The three
boxes indicate the locations of the fields used for the quantitative analysis:
region 1 within the undeprived SC and regions 2 and 3 at the border and in the
center of the scotoma, respectively. Scale bar, 200 µm. D,
Tridimensional reconstruction of the right SC (thin lines) containing the
collicular scotoma (thick lines). P, Posterior; A, anterior; M, medial; L,
lateral. E, F, Retinal fiber density at 7 and 21 d after the lesion.
Neither at the border (E) nor in the center of the scotoma
(F) are there significant differences between the two time points.
Error bars indicate SE. For each bar, n = 6 - 8 rats.
