The Journal of Neuroscience, August 6, 2003, ():

Transgenic Mice Expressing Green Fluorescent Protein under the Control of the Melanocortin-4 Receptor Promoter
J. Neurosci.
Liu et al. 23 (18): 7143.
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Qualitative estimates of GFP expression were made by considering the number of immunoreactive cells, as described in the Results section. The following five-point density scale was used; ++++, highest density; +++, high density; ++, moderate density; +, low density; –, undetectable. Asterisks denote that in the wild-type mice, MC4-R mRNA was undetectable in layer 1 of the cerebral cortex or in the subcortical structure.