The Journal of Neuroscience, September 3, 2003, ():
The Biological Clock Nucleus: A Multiphasic Oscillator Network Regulated by Light
J. Neurosci.
Quintero et al. 23 (22): 8070.
HTML Page - index.htslp
Files in this Data Supplement:
- Supplementary Figure
-
No effect of different levels of GFP expression on ionic currents in N2A cells. Top panel shows the mean relative intensity\ies of GFP in low and high expressing cells (N=8 each). Middle and bottom panels show mean current-voltage curves for the cells in the top panel. Middle panel shows peak currents, whereas the bottom panel shows steady state currents. There no significant differences between groups.
- Supplementary Video
-
Time-lapse confocal video of neuronal Per1::GFP rhythms in an SCN slice from an LD entrained animal. Fluorescence intensity is shown in false color with warmer colors (red) indicating higher intensities. This coronal SCN slice is oriented with the ventral aspect to the bottom of the frame and the medial aspect to the right. Recording begins in the early subjective day, when Per1::GFP expression is low in a majority of neurons, and continues through 24 hours with images taken every 20 minutes. It best viewed as a continuous loop. Salient features include the “twinkling” of cells as they peak at different phases, and the presence of two pairs of adjacent neurons that cycle in approximate antiphase to one another – one pair on the ventral/lateral quadrant of the SCN (lower left) and one pair in the dorsal/medial quadrant (upper right).
