The Journal of Neuroscience, October 29, 2003, ():
Role of the G-Protein Subunit 
-Gustducin in Taste Cell Responses to Bitter Stimuli
J. Neurosci.
Caicedo et al. 23 (30): 9947.
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Files in this Data Supplement:
- Supplemental Movie 1
-
Ca2+ response to stimulation with the bitter compound cycloheximide in a taste bud that was subsequently immunostained for a-gustducin. Confocal images of lingual slices showing 4 taste cells loaded with calcium green dextran (CaGD). One cell (arrow) responds to cycloheximide with an increase in CaGD fluorescence intensity (shift from blue to green in the pseudocolor intensity scale). This cell was immunoreactive for a-gustducin (red fluorescence). a-Gustducin was also present in other cells. Triangle at the level of the taste pore (top left) indicates the time of stimulus application. Movie duration: 2.5 min; frame interval: 2.5 sec. The immunostaining image is superimposed on the calcium-imaging recording at the beginning and at the end of the movie.
- Supplemental Movie 2
-
Ca2+ response to stimulation with the bitter compound cycloheximide in taste buds that were subsequently immunostained for a-gustducin. Confocal images of lingual slices showing 5 taste cells loaded with calcium green dextran (CaGD) in 2 different taste buds. One cell (arrow) responds to cycloheximide with an increase in CaGD fluorescence intensity (shift from blue to green to red in the pseudocolor intensity scale). This cell was immunoreactive for a-gustducin (red fluorescence). a-Gustducin was also present in other cells. Triangle (left) indicates the time of stimulus application. Movie duration: 2.5 min; frame interval: 2.5 sec. The immunostaining image is superimposed on the calcium-imaging recording at the beginning and at the end of the movie.
- Supplemental Movie 3
-
Ca2+ response to stimulation with the bitter compound cycloheximide in a taste bud that was subsequently immunostained for a-gustducin. Confocal images of lingual slices showing 3 taste cells loaded with calcium green dextran (CaGD). Two cells (arrow and arrowhead) respond to cycloheximide with an increase in CaGD fluorescence intensity (shift from blue to green to red in the pseudocolor intensity scale), but only one cell (arrowhead) was immunoreactive for a-gustducin. Triangle (top left) indicates the time of stimulus application. Movie duration: 2.5 min; frame interval: 2.5 sec. The immunostaining image is superimposed on the calcium-imaging recording at the beginning and at the end of the movie.
