The Journal of Neuroscience, November 5, 2003, ():
Multipolar Migration: The Third Mode of Radial Neuronal Migration in the Developing Cerebral Cortex
J. Neurosci.
Tabata and Nakajima 23 (31): 9996.
HTML Page - index.htslp
Files in this Data Supplement:
- Supplemental Movie 3a
-
An animated version of Figure 3a recorded for 7 hours (30 minutes interval).
The movement of the multipolar cells was observed on living slices prepared from EF1a-GFP/E12.5:E14 brains. Multipolar cells advanced toward the pial surface (upper side) very slowly, while dynamically extending and retracting multiple processes.
- Supplemental Movie 3b
-
An animated version of Figure 3b recorded for 14.5 hours (30 minutes interval).
DsRed-positive multipolar cells were frequently colocalized with DsRed-positive radial glial cells. In this specimen, three labeled multipolar cells were closely colocalized with a labeled radial glial cell, whose cell body was undergoing mitosis (M phase at t=7.5 hr). One of the multipolar cells advanced toward the pial surface by means of the dynamic movement of its processes and passed another multipolar cell.
- Supplemental Movie 3c
-
An animated version of Figure 3c recorded for 11.5 hours (30 minutes interval).
The locomotion cells within the CP were observed on the living slices taken from the CAG-GFP/E14.5:E18 brains
- Supplemental Movie 4b
-
An animated version of Figure 4b recorded for 13.5 hours (30 minutes interval).
Tangential “jump” of multipolar cells. One multipolar cell temporarily assumed locomotion-cell-like morphology by extending a thick process tangentially and jumping in that direction (t=2.5-8.5hr). The cell then retracted the thick tangential process and extended a radially oriented thick process. Finally, the cell resumed radial multipolar migration toward the pial surface (upper side) (t=10.5-14.0hr). The other cell (pointed by arrowhead in the printed version, t=6.5) also jumped tangentially (t=4.5-8.5hr).
