The Journal of Neuroscience, November 12, 2003, ():
Chromosome Segregation Defects Contribute to Aneuploidy in Normal Neural Progenitor Cells
J. Neurosci.
Yang et al. 23 (32): 10454.
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Lagging chromosomes in a live NPC mitosis lead to micronucleation. A-D, Prometaphase/metaphase. The cell in the dashed circle enters prometaphase at time 0:00 (denoting hour:minute). Prometaphase/metaphase lasts 160 min, accompanied by spindle rotations. E-G, Anaphase. Lagging chromosomes are visible during late anaphase (G; arrow). H, Telophase. Chromosome decondensation and nuclear envelope reformation take place, generating a micronucleus (arrow) enclosing lagging chromosomes in addition to two other daughter nuclei of unequal size. I, Interphase. Arrow indicates the micronucleus. Nuclear DNA is vitally stained with the cell-permeant dye Syto-16, which also labels mitochondrial DNA as bright dots. [Bar = 5 mm]
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A live NPC undergoes multipolar mitotic division. A-E, Prometaphase/metaphase. The cell in the dashed circle enters prometaphase at time 0:00 (denoting hour:minute). Prometaphase/metaphase lasts 120 min, accompanied by dynamic chromosome movements. F, G, Tripolar anaphase. Three segregating chromosome groups arise from this tripolar anaphase: the chromosome group circled in red is positioned approximately 4 mm above the other 2 groups circled in yellow (F). H, Tripolar telophase. I, Interphase. Nuclear DNA is vitally stained with the cell-permeant dye Syto-16, which also labels mitochondrial DNA as bright dots. [Bar = 5 mm]
