The Journal of Neuroscience, November 19, 2003, ():
Dopamine and Octopamine Differentiate between Aversive and Appetitive Olfactory Memories in Drosophila
J. Neurosci.
Schwaerzel et al. 23 (33): 10495.
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Files in this Data Supplement:
- Supplemental Figure 1
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Putative dopaminergic (DA) neurons innervating the mushroom body (MB) neuropil. TH-Gal4 / UAS-tau flies (Ito et al., 1997) were double stained with anti-TAU and the general neuropil marker antibody nc82 (curtsey E. Buchner, Würzburg). Complete3D data sets of adult brains were obtained by CLM microscopy and reconstructed using Amira and Standardbrain visualization software (Rein et al., 2002). (a) Transparent MBs (light blue, surface view) and tau-expressing fibers (yellow) within the MB neuropil. MBs were labeled manually, tau expressing fibers were detected by a threshold criterion (see Rein et al., 2002 for further details). In (b) a stack of 10 and in (c) of 30 virtual sections are compiled. MB neuropils are highlighted by white dotted contour lines. (b) The calyx is innervated only marginally by few non-branching fibers [arrows in (a) and (b)]. (c) In the heel [double arrow in (a) and (c)] and α-lobe [arrowhead in (a) and (c)] profusely arborizing fibers are found. Scale bar: 50 micro-meters.
