The Journal of Neuroscience, December 10, 2003, ():
Apoptosis Induced by p75NTR Overexpression Requires Jun Kinase-Dependent Phosphorylation of Bad
J. Neurosci.
Bhakar et al. 23 (36): 11373.
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Files in this Data Supplement:
- Supplemental Figure 1
-
Validation of Bad RNA Interference Vector. (A) PC12 cells were
transfected with increasing amounts of the U6-driven RNAi plasmid or a
corresponding parental control vector and then analyzed for reductions
in endogenous Bad, IkB alpha, Erk1/2 or Actin by immunoblot as
indicated. Bad levels were reduced but levels of IkB alpha, Erk1/2 and
Actin were unchanged. (B) Bad protein knockdown was quantified on
immunoblots by densitometry. Data shown represents average and standard
deviation of three independent experiments. U6 = pcDNA3 + U6 promoter,
U6-RL1 = pcDNA3 + U6 promoter driving Bad RNAi.
- Supplemental Figure 2
-
Cleaved Caspase 3 Immunoreactivity Correlates with TUNEL. PC12 cells
were exposed to staurosporine (10 ug/ml) or were infected with p75NTR
adenovirus (100 MOI) and 24 hours later were fixed and immunostained for
TUNEL and cleaved Caspase 3. Cells were scored for coincidence of TUNEL
and Caspase 3 cleavage by a blinded observer (n = 300 cells/condition).
