The Journal of Neuroscience, December 17, 2003, ():
Generation of Functional Radial Glial Cells by Embryonic and Adult Forebrain Neural Stem Cells
J. Neurosci.
Gregg and Weiss 23 (37): 11587.
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Files in this Data Supplement:
- Supplemental Figure 1
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EGF promotes the differentiation of RGCs generated by NSCs. A, B, Cells from dissociated EGF-generated E14 neurospheres, identified with the nuclear marker Hoechst (blue), that expressed the radial cell marker RC2 (green, arrowheads) did not adopt a radial morphology when differentiated in basal media for 14-16 hr. C, D, RC2-expressing cells differentiated in the presence of EGF (arrows) displayed a highly elongated, radial morphology. E, F, All radial RC2-expressing cells also expressed GLAST, thereby displaying both the neuroepithelial and astroglial characteristics indicative of RGCs. Scale bar, 20 mm.
- Supplemental Figure 2
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Closer analysis of the absence of RC2 + BLBP coexpressing cells in the subependyma of adult vehicle-infused control animals. A[en]D, Immunohistochemical analysis of RC2 and BLBP expression in vehicle-infused animals by double labeling revealed the complete absence of RC2 expression in the subependyma (A, arrowhead; B, close up of boxed area; n = 4; scale bar, 100 mm), unlike growth factor-infused animals in which RC2-expressing cells, having clear cellular morphologies, were present around the entire circumference of the ventricles (Figs. 6, 7). We cannot exclude the possibility that low levels of RC2 expression are present in the adult subependyma of vehicle controls that are below the level of detection of our immunohistochemical protocol. Although no RC2 immunoreactivity was observed in the subepedendyma, small amounts of diffuse RC2 immunoreactivity were observed in the corpus collosum of some but not all control animals (A, arrows; n = 2 of 4). We observed a small number of cells expressing BLBP in the subependyma of control infused animals (C, close up of boxed area in D; an example of a positive cell is indicated by the arrow in C and D), but these cells did not coexpress RC2.
- Supplemental Movie 1
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RGC processes generated within the adult subependymal zone of an EGF-infused mouse. This movie rotates confocal z-stacked images of RC2 immunolabeling (red) and provides a three-dimensional perspective of single, elongated RC2-expressing basal RGC processes that extend perpendicularly from the surface of the lateral ventricles into the striatum.
- Supplemental Movie 2
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Adult ependymal cells extend single basal processes in response to the activation of EGFR signaling. This movie rotates confocal z-stacked images providing a three-dimensional perspective of single, basal processes extending from adult ependymal cells residing around the lateral ventricles (s100[beta] labeling shown in green) in response to the infusion of EGF.
