The Journal of Neuroscience, April 1, 2003, ():
Imaging Calcium Entry Sites and Ribbon Structures in Two Presynaptic Cells
J. Neurosci.
Zenisek et al. 23 (7): 2538.
Movies
Files in this Data Supplement:
- Movie 1
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Evanescent field fluorescence movie of a bipolar cell loaded with 200 mM Fluo-5f and 10 mM EGTA. Calcium current is elicited by a step depolarization to 0 mV from a resting potential of -60 mV. Depolarization is coincident with square in upper left hand corner. Time is 1/3rd real speed; scale bar is 2 mm. Same cell as in figure 1 A - F.
- Movie 2
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Same as movie 1 for different cell. Movie is shown at actual speed; scale bar is 2 mm. Same cell as in figure 1 G, H.
- Movie 3
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Evanescent field fluorescence movie of a hair cell loaded with 200 mM Fluo-3 and 10 mM EGTA. Calcium current is elicited by a step depolarization to 0 mV from a resting potential of -60 mV. Note that during the depolarization fluorescence is localized to two spots within the cell footprint. Depolarization is coincident with square in upper left hand corner. Time is one-half actual speed; scale bar is 5 mm.
- Movie 4
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Same movie as movie 3, except with contrast exaggerated so that fluorescent “flickers” are more readily visible. Arrows show time and location of flickers.
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