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The Journal of Neuroscience, May 5, 2004, ():

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Interplay between Local GABAergic Interneurons and Relay Neurons Generates {gamma} Oscillations in the Rat Olfactory Bulb
J. Neurosci. Lagier et al. 24: 4382

Supplemental data

Supplementary Figures

Files in this Data Supplement:

  • Supplementary Fig. 1 - Supplemental Figure 1. Two examples of normalized PSTH processed on 40 consecutive sweeps of simultaneous GCL and MCL multiunit recordings. Left plots, PSTH calculated on 4 sec with bins of 5 msec. The dashed lines indicate the level of basal activity. Right plots, PSTH calculated on 500 msec (corresponding to the gray boxes in the left plots) with 2 msec bins. The dashed lines indicate the level of basal activity. The red lines are bimodal exponentially decaying fits. Offsets are null for both GCL unit PSTH fits (?2 test; p < 0.005 in both cases) and equal to 0.21 and 0.17 for MCL units 1 and 2, respectively (?2 test; p < 0.05 in both cases).
  • Supplementary Fig. 2 - Supplemental Figure 2. A1, Simultaneous mitral/tufted cell intracellular (Vm) and LFP recordings. The intracellular recording pipette contained K-acetate (KAc, 2 M). The cell was hyperpolarized to prevent spike emission (the holding membrane potential is indicated in parentheses). The star indicates the stimulation onset. A2, Enlargement of the sweeps presented in A1 (dashed boxes). Arrows point to hyperpolarizing, putative GABAergic synaptic events. B1, Simultaneous mitral/tufted cell intracellular (Vm) and LFP recordings with KCl (2 M) in the intracellular recording pipette. The cell was hyperpolarized to prevent spike emission. B2, Enlargement of the sweeps presented in B1 (dashed boxes). Arrowheads point to depolarizing events occurring at the same frequency as hyperpolarizing events seen in A2. C, Sensitivity of events amplitude to the holding voltage. Hyperpolarizing events recorded in KAc (2 M) are presented as open circles, and depolarizing events recorded in KCl (2 M) are presented as filled circles. The inferred reversal potential of these events is at 105 and +3 mV in KAc and KCl recording conditions, respectively. The slope of the linear regressions is 30 and 24 fV/mV; p < 0.05 and 0.005 in KAc and KCl recording conditions, respectively; n = 5 cells in both conditions. D, Intracellular recordings of mitral/tufted cells in KCl (2 M) under different pharmacological conditions. Depolarizing events (arrowheads) disappeared after SR95531 application and reappeared after a wash of the drug in all five recorded mitral/tufted cells.




This Article
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