The Journal of Neuroscience, June 9, 2004, ():
Microdomains for Dopamine Volume Neurotransmission in Primate Prefrontal Cortex
J. Neurosci. Paspalas and Goldman-Rakic
24: 5292
Supplemental data
Supplemental Figures
Files in this Data Supplement:
- Supplemental Fig. 1
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Supplemental Figure 1. Positive immunocytochemical controls for the InsP3R (arrows; nanogold two-layer immunocytochemistry; see Materials and Methods) with a polyclonal antibody raised against a short amino acid sequence of the human InsP3R C-terminal domain. The immunogen peptide for this antibody shows no sequence homology with the peptide used for raising the InsP3R monoclonal antibodies used in dual immunocytochemistry (Table 1). Note the distinctive immunoreactivity of the spine apparatus (asterisk) in A (compare with Fig. 5A) and the selective labeling of cisternal (B) and other tubulo-vesicular SER endomembranes (C, D; compare with Fig. 5B,D). Double arrowheads point to SER profiles, and arrowheads point to synaptic vesicles within one of the rarely visualized InsP3R immunoreactive axonal varicosities. ax, Axon; den, dendrite. Scale bars, 200 nm.
- Supplemental Fig. 2
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Supplemental Figure 2. Particle-size segregation in the pilot experiments. Gold particles produced from the sequential nanogold enhancement comprised two distinct, nonoverlapping size groups with a mean diameter differing from 300 to 450% (arrows vs arrowheads). In A and B, nanogold-conjugated secondary antibodies were directly linked on tissue with glutaraldehyde (no primary antibody). C--E, Dual immunolabeling with mouse (Sigma, St. Louis, MO; or Swant, Bellinzona, Switzerland) and rabbit (Swant) anti-parvalbumin primary antibodies, followed by species-specific nanogold-secondary antibodies. The next panels illustrate dual immunolabeling with rabbit anti-D5R (F, arrowheads) and rat anti-D1R (F, arrows) or rat anti-D1R (G, arrowheads) and rabbit anti-InsP3R (G, arrows). Double arrowheads in F point to a perforated asymmetric synapse. ax, Axon; den, dendrite; sp, spine; *, spine apparatus. The detailed immunoprocedures are described in Materials and Methods. Scale bars: A--G, 200 nm; E, inset, 25 nm.
