The Journal of Neuroscience, June 23, 2004, ():
Control of Roof Plate Development and Signaling by Lmx1b in the Caudal Vertebrate CNS
J. Neurosci. Chizhikov and Millen
24: 5694
Supplemental data
Supplemental Figures
Files in this Data Supplement:
- Supplemental Fig. 1
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Figure 1. Lmx1b overexpression does not affect proliferation of neural progenitors in chick embryos. BrdU immunohistochemistry (red) alone (A) or together with GFP visualization (green) (B) in chick developing spinal cord 48 h.a.e. C, Higher magnification of the neural tube shown in B. D, Quantification of proliferation of cells expressing exogenous Lmx1b and EGFP alone in chick embryonic spinal cords 48 h.a.e.
- Supplemental Fig. 2
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Figure 2. Effects of Bmp4 and Wnt1 overexpression on chick dorsal spinal cord specification. Effect of Bmp4 (A--E) and Wnt1 (F--J) overexpression on expression of Pax7 (A, F), Pax6 (B, G), and dI1 (C, H) and dI4--dI6 (D, I) interneuron development. Insets show higher magnifications of selected neuronal populations. Quantitative analysis of numbers of dI1 and dI4--dI6 interneurons in neural tubes electroporated with Bmp4 (E) and Wnt1 (J).
- Supplemental Fig. 3
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Figure 3. Lmx1b is not involved in dI5 and dILB interneuron specification. A--D, Overexpression of Lmx1b in chick developing spinal cord has no effect on Lbx1 (E, F) and Brn3a (G, H) expression. E--H, Visualization of dI5 and dILB interneurons in dorsal spinal cords of wild-type (wt) and Lmx1b[minus]/[minus] embryos by Lbx1 and Lim1 double staining at the indicated stages.
