The Journal of Neuroscience, June 30, 2004, ():
Retroinhibition of Presynaptic Ca2+ Currents by Endocannabinoids Released via Postsynaptic mGluR Activation at a Calyx Synapse
J. Neurosci. Kushmerick et al.
24: 5955
Supplemental data
Supplementary Figure
Files in this Data Supplement:
- Supplemental Fig. 1
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Supplemental Figure 1. Controls for the immunohistochemistry results. A, Positive control for CB1 staining using the cerebellum. The pre-embedding immunoperoxidase method shows CB1 immunoreactivity in the cerebellum of P14 rats. Granule cells (arrowheads) and some Golgi cells (asterisk) are stained. B, Positive control for CB1 staining using the interpeduncular nucleus. CB1 immunoreactivity is clearly seen in the interpeduncular nucleus of P14 rats and is limited to this tissue. The intensely stained dense mesh of fibers within the interpeduncular nucleus creates a sharp edge with respect to the surrounding tissue. C, Negative control for the DAB peroxidase method of detection. No immunoreactivity is seen in the P14 MNTB after substitution of primary antiserum by nonimmune serum, indicating that the staining patterns generated for mGluR1a and CB1 immunoreactivity are specific.
