The Journal of Neuroscience, July 21, 2004, ():
Stereotyped Odor-Evoked Activity in the Mushroom Body of Drosophila Revealed by Green Fluorescent Protein-Based Ca2+ Imaging
J. Neurosci. Wang et al.
24: 6507
Supplemental data
Supplementary Figure
Files in this Data Supplement:
- Supplemental Fig. 1
-
Supplemental Figure 1. Identifying KCs with odor-evoked large G-CaMP fluorescence
transients. A, Original fluorescent images taken in the KC somata region at a depth of
12 m before and during stimulation by 10?3 benzaldehyde. KCs with large
fluorescence increases are clearly visible. B, Processing of ?F images of benzaldehyde evoked G-CaMP fluorescence transients shown in A with different threshold levels. The ?F image was obtained by subtracting the average of 10 background fluorescence images taken before odor stimulation from the average of three fluorescence images at the peak of the G-CaMP fluorescence response. The ?F image was further filtered pixel by pixel with a threshold corresponding to 2, 3, and 4' SEM of fluorescence fluctuation at each pixel before odor stimulation. C, ?F image of fluorescence activities in response to air control thresholded pixel by pixel with 3' SEM of fluorescence fluctuations at each pixel. D, Histograms of the amplitudes of the fluorescence intensity changes in 30 KC somata including the three marked KCs in B in response to air control (dark bars) as well as in 30 randomly chosen presumed nonresponsive KCs in response to benzaldehyde stimulation (light gray bars). Data were pooled from five odor trials and five air controls recorded at different depths of the brain in a single fly. The peak amplitudes of benzaldehyde-evoked fluorescence transients in the three KCs labeled as 1, 2, and 3 in B are 23.2, 67.6, and 35.9%, respectively. Along with seven other KCs showing a similar range of fluorescence transients, they are significantly above the fluorescence activities in air control and in randomly chosen presumed nonresponsive KCs (Student’s t test; p < 0.001).
