The Journal of Neuroscience, August 4, 2004, ():
Coordinating Structural and Functional Synapse Development: Postsynaptic p21-Activated Kinase Independently Specifies Glutamate Receptor Abundance and Postsynaptic Morphology
J. Neurosci. Albin and Davis
24: 6871
Supplemental data
Files in this Data Supplement:
- supplemental Fig. 1
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Supplemental Figure 1. Decreased GluRIIA abundance at the newly formed NMJ in Pak mutant animals. A, NMJs from first-instar wild-type, Pak6, and Pak3 larvae that are stained with anti-Dlg (green; top row) and anti-GluRIIA (red; middle row). Merged images are shown in the bottom row. GluRIIA abundance at the NMJ is decreased in all Pak mutants in the first instar. Images are shown at the identical calibration. B, Wild-type first-instar synapse stained with anti-Pak (green; top) and anti-GluRIIA (red; middle). The merged image shows colocalization of the two proteins (bottom). C, Quantification of the GluRIIA fluorescence intensity in Pak mutants, expressed as a percentage of wild type. Pak3, Pak4, and Pak6 animals show a decrease in GluRIIA levels at the synapse (Pak3, 72.5 ± 1.3%, n = 3; Pak, 72.7 ± 5.2%, n = 7; Pak6, 42.3 ± 3.9%, n = 5; *p [lt] 0.002; Student’s t test). D, Cumulative probability curve of the maximum GluRIIA staining of individual GluRIIA clusters in wild-type and Pak4 mutant animals. The x-axis values are in arbitrary fluorescence units. E, Average GluRIIA receptor cluster density per synapse area was calculated for wild-type (4.8 ± 0.5) and Pak6/Df (4.5 ± 0.4; n = 11). There is no change in this parameter comparing these genotypes.
