The Journal of Neuroscience, August 11, 2004, ():
A Conserved Role But Different Partners for the Transcriptional Corepressor CoREST in Fly and Mammalian Nervous System Formation
J. Neurosci. Dallman et al.
24: 7186
Supplemental data
Files in this Data Supplement:
- Supplemental Fig. 1 -
Supplementary Figure 1. Because the Ttk88 binding sites are only 7 bp long and have not been characterized extensively, we compared the incidence of these sites in D. melanogaster and D. pseudoobscura for genes identified as potential Ttk88 targets by chromatin immunoprecipitation. The present analysis of Ttk69 sites is most likely an underestimate, because we only searched with a single consensus site. The number and distribution of Ttk88 binding sites in para, engrailed, and -tubulin genes are similar between D. melanogaster and D. pseudoobscura. For both synapsin and ELAV, there is an asymmetry in the number of sites between D. pseudoobscura and D. melanogaster, but the distribution of the sites is similar. The organization of the stathmin SCG10-like has changed dramatically in these two species, and so a direct comparison cannot be drawn. This comparison lends support to the idea that Ttk88 might regulate these genes.
- Supplemental Fig. 2
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Supplemental Figure 2. Ttk and REST are likely functional replacements of one another. Genome-wide searches for REST and Ttk show that REST homologs are restricted to the vertebrate lineage, that Ttk homologs are restricted to the fly lineage, and that neither REST nor Ttk occur in the nematode lineage. These searches were complicated by the fact that REST and Tramtrack are both zinc finger proteins, a large gene family in most organisms. Therefore, when searching for orthologs, we used other functional domains, such as the Sin3 and CoREST interacting domains of REST and the BTB domain of Tramtrack. Our findings raise the possibility that these structurally unrelated proteins are functionally replacing one another in their respective organisms. Numbers at the branch points are in millions of years.
