The Journal of Neuroscience, October 6, 2004, ():
Switching of NMDA Receptor 2A and 2B Subunits at Thalamic and Cortical Synapses during Early Postnatal Development
J. Neurosci. Liu et al.
24: 8885
Supplemental data
Files in this Data Supplement:
- supplemental material
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Supplemental figure 1: P19 cortical sections are stained with NR2B antibody using postembedding immunogold electron microscopy. A-C: Serial electron micrographs show synaptic and non-synaptic NR2B localization. Boxed regions in B and C show two immunogold particles consistently present at a PSD of an asymmetrical synapse in adjacent sections. Insets show high power views of particles at PSD. Large arrows indicate NR2B particles localized at extrasynaptic regions but they can not be traced in serial sections. Bar in C: 0.2ìm; bar in the B inset: 0.02ìm. D-G: Serial electron micrographs show NR2B immunogold particles associated with extrasynaptic (regions 1, 2, 3 and 4, large arrows in D, E, F). Insets are high power images showing particles in regions 1, 2, 3. Note the immunogold labeling in each region can not be detected at corresponding regions in adjacent sections. Bar in G: 0.2ìm; bar in the D inset: 0.1ìm.
- supplemental material
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Supplemental figure 2: Immunofluorescent colocalization of PSD-95 (green, Alexa-488) and GABA (red, Alexa-647) in somatosensory cortex (CTX), hippocampal region CA1, the thalamic reticular nucleus (RTN), the medial habenula (mHab) and substantia nigra (SN). Tissue was counterstained with DAPI (blue) to delineate brain regions. In both cortex and hippocampus, PSD-95 (open arrowhead) was not expressed in GABAergic (closed arrowheads) neurons. Note the strong expression of PSD-95 in apical dendrites of cortical pyramidal neurons (arrows) and pyramidal neurons of CA1 (arrows). Very little PSD-95 was expressed in RTN, which is exclusively GABAergic, while PSD-95 expression was heavy in mHab, where little GABA expression was found. In contrast, some GABAergic neurons in substantia nigra expressed PSD-95 (asterisks). Bar: 100|*mu*|m.
