The Journal of Neuroscience, October 13, 2004, ():
Optical Postsynaptic Measurement of Vesicle Release Rates for Hippocampal Synapses Undergoing Asynchronous Release during Train Stimulation
J. Neurosci. Otsu and Murphy
24: 9076
Supplemental data
Videos 1,2, and 3: mpeg videos representing 20 s of continuous imaging. The videos were made from the cell shown in Fig. 3A. The first image of each is a mag-fura-2 image (unstimulated to show dendrite structure). The axon of the cell is also labeled but is not visible because of its significantly smaller volume than the dendrite. The next 300 images (~20 s) are raw fluo-4 fluorescence (median filtered 2x2): a baseline was taken for 2.2 sec, followed by a train 20 Hz for 0.5 s (beginning at image #33), and asynchronous release monitored for the next 17 s in the presence or absence of NMDAR blockers to discriminate between Ca2+ signals mediated by NMDAR activity and other sources including action potentials (APs).
Files in this Data Supplement:
- supplemental material
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Video 1, AP mediated dendritic Ca2+ transients. Train stimulation in the continuous presence of NMDAR blockers showing only the contribution of voltage gated Ca2+ channels activated as a consequence of evoked transmitter release. Somatic APs that trigger transmitter release back propagate into the dendrites since these neurons have autaptic synapses. No asynchronous miniature synaptic calcium transient (MSCT) events were observed under these conditions.
- supplemental material
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Video 2, AP mediated dendritic Ca2+ transients and asynchronous NMDAR synaptic activity. Response recorded with NMDAR blockers washed out just after train stimulation to promote asynchronous NMDAR activity. MSCTs are observed over a background of AP mediated Ca2+ signals.
- supplemental material
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Video 3, (Video 2-Video 1, difference sequence) shows asynchronous MSCT events better because the AP-mediated component has been subtracted out.
