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The Journal of Neuroscience, October 20, 2004, 24(42):9282-9290; doi:10.1523/JNEUROSCI.2120-04.2004
Previous Article | Next Article
Development/Plasticity/Repair
Astrocyte-Associated Fibronectin Is Critical for Axonal Regeneration in Adult White Matter
Veronica J. Tom,1 Catherine M. Doller,1 Alfred T. Malouf,1,2 andJerry Silver1 Departments of 1Neurosciences and 2Pediatrics, Case Western Reserve University, Cleveland, Ohio 44106
Abstract
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Abstract
Introduction
Although it has been suggested that astroglia guide pioneering axons during development, the cellular and molecular substrates that direct axon regeneration in adult white matter have not been elucidated. We show that although adult cortical neurons were only able to elaborate very short, highly branched, dendritic-like processes when seeded onto organotypic slice cultures of postnatal day 35 (P35) rat brain containing the corpus callosum, adult dorsal root ganglion (DRG) neurons were able to regenerate lengthy axons within the reactive glial environment of this degenerating white matter tract. The callosum in both P35 slices and adult rat brain was rich in fibronectin, but not laminin. Furthermore, the fibronectin was intimately associated with the intratract astrocytes. Blockade of fibronectin function in situ with an anti-fibronectin antibody dramatically decreased outgrowth of DRG neurites, suggesting that fibronectin plays an important role in axon regeneration in mature white matter. The critical interaction between regrowing axons and astroglial-associated fibronectin in white matter may be an additional factor to consider when trying to understand regeneration failure and devising strategies to promote regeneration.
Key words: astrocyte; astroglia; callosum; dorsal root ganglion; DRG; fibronectin; regeneration; slice
Introduction
The guiding role of astroglia in axonal navigation was initially proposed after the observation that oriented glial structures presage the route of pioneering populations of axons during embryonic development (Silver et al., 1993; Pires-Neto et al., 1998
In addition to their patterned geometry, astrocytes are thought to provide both growth-promoting and growth-inhibiting molecules, the balance of which within the environment of navigating axons can either attract or deflect outgrowth. Various growth-promoting molecules, including laminin (Liesi, 1985
To better understand the cellular and molecular mechanisms that foster robust regeneration of adult axons through mature white matter, we developed an in situ slice culture system to study axon regeneration in a living, easily manipulated, three-dimensional environment. Just as they do after microtransplantation in vivo, dissociated adult dorsal root ganglion (DRG) neurons seeded onto postnatal day 35 (P35) coronal organotypic slice cultures of cortex containing the corpus callosum grow extremely well in this heavily myelinated white matter tract. Contrary to previous findings, the fully mature callosum is rich in astrocytic-associated fibronectin that appears to be critical for sensory axon outgrowth. Our findings suggest that astroglial-associated fibronectin in adult white matter tracts may be a critical extracellular molecule (ECM) that is provided to severed axons in the CNS. Whether adult axons are capable of responding maximally to this readily available extracellular substrate may be an additional factor to consider when trying to understand regeneration failure as well as devising strategies to promote regeneration.
Materials and Methods
DRG dissociation. DRG neurons were obtained as described previously (Davies et al., 199730 ganglion from the cervical, thoracic, and lumbar segments of the spinal cord), the roots were trimmed, and the ganglia were incubated in dispase-collagenase for 1.5 hr at room temperature (RT). Afterward, the ganglia were washed several times in calcium and magnesium-free-HBSS (CMF) and then gently triturated. The cell suspension was spun several times to help remove satellite glia (
Cortical neuron dissociation. Adult cortical neurons were obtained using a modified method of Brewer (1997
Organotypic slice culture. P35 Sprague Dawley rats were anesthetized and decapitated. The brains were quickly removed from the skull, blocked, and glued onto a sterile pedestal. Coronal slices (200 µm) containing the corpus callosum were cut in ice-cold CMF. Each slice was then placed on a 25 mm tissue culture insert with a 0.02 µm pore size (Nunc, Naperville, IL) in Neurobasal A/B27 (Invitrogen, San Diego, CA) with 100 µM CNQX and 100 µM AP-5 to block glutamate receptor-mediated toxicity (Hafidi et al., 1999
Tissue culture. Coverslips were coated with poly-L-lysine (1 mg/ml) overnight, rinsed several times with sterile water, and allowed to dry. Some coverslips were then incubated with fibronectin (10 µg/ml) or laminin (10 µg/ml). The remaining coverslips were not coated with anything other than poly-lysine. Freshly dissociated DRG neurons were plated onto the coverslips. Two hours after cell plating, cultures on the fibronectin-coated coverslips were treated with either anti-fibronectin or rabbit IgG (concentrations as mentioned above), and cultures on the laminin-coated coverslips were treated with either anti-laminin or rabbit IgG. To control for antibody-induced toxicity, cultures on the polylysine coverslips were treated similarly.
Cortical astrocyte dissociation. Astrocytes were obtained from P1 Sprague Dawley rats. The pups were decapitated, and the cortices were removed, finely minced, and treated with 0.1% trypsin in EDTA. Cells were rinsed in serum-containing media, gently spun down, and then seeded onto poly-lysine-coated flasks in DMEM/F12 with 10% FBS. Four hours later, the media was changed after the flasks were shaken to remove nonadherent cells. The astrocytes were maintained for 5 weeks, when they were gently trypsinized from the surface of the flasks, rinsed in fresh media, and plated on poly-lysine-coated coverslips at a density of 75,000-100,000 cells per coverslip. The next day, RIgG, anti-fibronectin, or anti-laminin was added to the media. The cultures were maintained for an additional 3 d before fixation in 4% paraformaldehyde.
Immunohistochemistry. Organotypic slice cultures were immersion fixed in 4% paraformaldehyde in 0.1 M PBS for 2 hr. Cultures on cover-slips were fixed in 4% paraformaldehyde for 30 min. In addition, rats were transcardially perfused with ice-cold 4% paraformaldehyde in PBS, and the brains were dissected out. After the tissue was postfixed in 4% paraformaldehyde overnight at 4°C, 50 µm sections were cut on a vibratome. After several rinses in PBS, coverslip cultures were blocked with 5% NGS, 0.1% BSA plus 0.1% Triton X-100 in PBS for 1 hr at RT before they were incubated in primary antibody overnight at 4°C. Slice cultures and brain sections were blocked with 3% NGS, 3% BSA, 0.1% DMSO, and 0.1% Triton X-100 (if the antigens were intracellular) in PBS for 1 hr at RT before they were incubated in primary antibody overnight at 4°C. The primary antibodies used were rabbit anti-GFP (1:1000; Chemicon), mouse anti-GFAP (1:500; Sigma, St. Louis, MO), mouse anti-myelin (MAB328, 1:1000; Roche Products, Hertfordshire, UK), mouse anti-
-tubulin type III (1:1000; Sigma), rabbit anti-fibronectin (1:400; Dako), and rabbit anti-laminin (1:1000; Biomedical Technologies). The cultures were rinsed in PBS and incubated in the appropriate secondary antibody either for 1.5 hr at RT (coverslips) or overnight at 4°C (slice cultures and sections). They were rinsed again in PBS, mounted in Citifluor (Ted Pella), coverslipped, and sealed with nail polish. Specimens were examined with a Leitz Orthoplan 2 microscope and a Zeiss (Oberkochen, Germany) LSM 410 confocal microscope.
Quantification. To quantify axonal outgrowth on fibronectin- or laminin-coated coverslips treated with RIgG, rabbit anti-fibronectin, or rabbit anti-laminin, montaged images were taken of semirandomly chosen neurons (n = 12 per group) maintained for 3 d in culture and stained for
To quantify outgrowth of neurons attached to either gray or white matter of the slices, confocal images were taken of the corpus callosum and the gray matter just adjacent to the callosum (n = 4 slices). Montages were made from the images, and all individual neurites that could be followed were digitally traced by hand using a consistent brush thickness that was approximately the width of an average neurite (Photoshop). The tracings alone were quantified for pixel area (MetaMorph), which was then divided by the number of cells extending neurites on gray or white matter to get a neurite length per cell average, which accounts for any differences in the numbers of cells that have attached on gray versus white matter. This process also eliminates any subtle differences in neurite width so that the only variable is the length of the neurites. These pixel area averages were analyzed using a Student's t test for statistical relevance.
Outgrowth of neurites on white matter of slices treated with RIgG, anti-fibronectin, or anti-laminin was quantified similarly. Confocal images were taken of areas of the corpus callosum containing GFP+ neurites in RIgG-treated (n = 10, control for anti-fibronectin; n = 5, control for anti-laminin), anti-fibronectin-treated (n = 10), and anti-laminin-treated (n = 5) cultures. Montages were made from the images, and individual neurites were digitally traced using a consistent brush thickness that was approximately the width of an average neurite (Photoshop). The tracings were quantified for pixel area (MetaMorph), which was then divided by the number of cells (on the order of approximately two to three dozen per slice) that extended neurites to get a cell average. These pixel area averages were analyzed using a Student's t test for statistical relevance.
Results
Robust regeneration of adult sensory neurons on the living corpus callosum in culture
Coronal organotypic slices of the cerebrum from P35 rats (Fig. 1A,B, shown in bright-field at low magnification) were able to support the adherence of adult sensory neurons, especially in the region that included the callosum (Fig. 1C). Moreover, the attached neurons were able to regenerate lengthy axons only within GFAP+ astrocyte-rich regions (Fig. 2A, arrowhead; GFAP in red, GFP+ neurites in green) (see below for a quantification of axonal outgrowth). The sensory neurons attached less well to gray matter. When they did, they elaborated very short neurites (Fig. 2B, arrow, green). This significant difference in neurite outgrowth from neurons attached to either gray or white matter is quantified in Figure 2E (p < 0.005). The P35 gray matter, unlike the callosum, after 4 d in culture was filled with necrotic debris and devoid of any GFAP+ astrocytes, suggesting that the gray matter astrocytes either had died or (albeit unlikely) had dedifferentiated into a non-GFAP-expressing state.
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Figure 1. Dissociated adult DRGs attach to organotypic slices containing white matter. A, Low-magnification bright-field image of an organotypic coronal slice containing the white matter tract the corpus callosum that had been in culture for 4 d. B, C, Dissociated GFP+ DRGs were seeded onto the callosal region of 1 DIV slices and fixed and stained at 4 DIV. The bright-field image of the slice is shown in B. The fluorescent image of the GFP+ neurons is shown in C. The white matter tract is denoted by the white lines. Note that many neurons were able to adhere to the white matter. Scale bars: A, 500 µm; B, C, 200 µm.
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Figure 2. DRGs regenerate robustly on the corpus callosum. A, Confocal microscopy montage of GFP+ DRGs (green) on the GFAP+ astrocyte-rich corpus callosum (red). Insets are higher-magnification images of areas denoted by the white boxes. The DRGs were able to grow well on the callosum and extended long neurites (C, arrowhead) after 3 DIV; however, neurons on gray matter, which was virtually devoid of GFAP+ astrocytes, were able to extend only very short neurites (B, arrows in main figure A). Although much of the growth appeared to grossly follow the alignment of the host astrocyte network, there were numerous instances during which axons strayed and turned (C, D, arrowhead). E, Quantification of the disparity between outgrowth on gray and white matter. Neurites from DRGs seeded onto 1 DIV organotypic slice cultures and maintained for 3 DIV were traced digitally. Neurites originating from DRGs seeded on gray matter of the organotypic slice culture after 3 DIV were traced digitally and compared with those neurites that grew on the corpus callosum (n = 4 slices per group). The pixel areas of the tracings were quantified and analyzed statistically using a Student's t test. There were significantly shorter neurites per neuron on gray matter than on white matter (*p < 0.005). Scale bar, 100 µm.
Adult cortical neurons regenerate poorly on the corpus callosum in organotypic slices
Although the corpus callosum provided a highly growth-supportive environment for adult sensory neurons, it turned out to be a poor substrate for the outgrowth of adult cortical neurons. Adult GFP+ cortical neurons (Fig. 3A,C,D,F, green) seeded on the white matter tract were able to generate highly branched processes; however, they were relatively short, and there was never a clear-cut lengthy axon. Every process that extended from the cell body contained MAP-2, a dendritic marker (Fig. 3B,C,E,F, red).
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Figure 3. Cortical cells do not regenerate well on white matter. Adult cortical neurons seeded onto the corpus callosum of P35 organotypic slice cultures were double stained for GFP (A, C, D, F, green) and the dendritic marker MAP-2 (B, C, E, F) after 8 DIV. Even after such a long time in culture, the neurons were able to extend only short neurites. Furthermore, all neurites were positive for MAP-2, suggesting that they were of a dendritic nature. Scale bar, 25 µm.
Sensory axon regeneration on the callosum occurs despite the presence of degenerating myelin
There have been a great number of studies suggesting that myelin and/or inhibitory myelin proteins released on the degeneration of lesioned axon tracts inhibit cell attachment and axonal regeneration (for review, see Caroni et al., 1988
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Figure 4. DRGs regenerate neurites despite the presence of myelin. A, B, An abundance of myelin was present in the corpus callosi of both 1 DIV slices (when neurons were seeded) and 4 DIV slices (when cultures were fixed and regeneration was assessed). C, D, Z-series of confocal microscope images revealed that although there were some GFP+ neurites that remained on the surface of the slice (D, green, arrowhead), the majority of regenerating neurites penetrated and grew
Neurites appear to crudely follow host astrocyte alignment
In our assay, we found that the regenerated neurites followed the alignment of the host astrocytes only very generally. When the host astrocytes ran generally parallel in the corpus callosum (Fig. 5A,B, red), the neurites grew essentially in the same direction (Fig. 5A,B, arrowheads, green). When the host astrocytic processes extended radially in the cingulum (Fig. 5C,D, red), the neurites grew in that direction (Fig. 5C,D, arrows, green). This suggests that the orientation of the regenerating neurites is somewhat influenced by the alignment of the host astrocytes; however, it should be stressed that the neuritic alignment with astrocytes was only crudely aligned, at best, resulting in axon growth that meandered from one astrocytic process to another (Figs. 2C,D, 5B, asterisk).
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Figure 5. Regenerating neurites are only crudely aligned with host tract astrocytes. A, B, regenerating GFP+ neurites (green) generally followed host GFAP+ astrocytic processes in the callosum (red) and grew longitudinally (arrowheads). Alignment was not exact, however, and there were instances during which neurites wandered and jumped from one astrocytic process to another (B, asterisk). C, D, Regenerating neurites (green) in the cingulum grew radially away from the callosum, apparently guided by the astrocytes that were present there (arrows). Scale bar, 25 µm.
Fibronectin is expressed by callosal astrocytes
To determine which growth-promoting factor might reside on the surface of the mature astrocyte, fresh, living slices were immediately fixed and stained for both laminin and fibronectin, two growth-promoting ECMs known to be made by immature astrocytes (Liesi et al., 1983
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Figure 6. Fibronectin, not laminin, is expressed by astrocytes in the corpus callosum. A,B, Freshly fixed coronal slices stained for fibronectin (A) and laminin (B). Although laminin was visualized only along blood vessels, fibronectin was expressed all over the cortex, including the corpus callosum, demarcated by the blue dots. C, D, Perfused adult rat brain sections stained for fibronectin (C) and laminin (D). Again, the callosum was rich in fibronectin, whereas laminin was present only along blood vessels. E-G, A GFAP+ astrocyte in the callosum of a slice (F, G, red) was closely associated with fibronectin-expression patterns (E, G, arrows, green). Scale bars: A-D, 50 µm; E-G, 5 µm.
Polyclonal fibronectin and laminin antibodies block axonal outgrowth on their respective purified substrates
To determine what role fibronectin plays in neurite outgrowth in white matter, we first needed to ensure that the antibody that we were using to detect fibronectin was function blocking. Dissociated DRGs were plated onto purified fibronectin or laminin-coated coverslips, and after 2 hr to allow for neuronal attachment, the polyclonal fibronectin antibody, the polyclonal laminin antibody, or RIgG was added to the media. Three days later the cultures were fixed, and neurite outgrowth was assessed. DRGs incubated with RIgG extended long neurites (Fig. 7A,G); however, both the fibronectin antibody (Fig. 7B,G)(*p < 0.00001) and the laminin antibody (Fig. 7C,G) (**p < 0.00000001) dramatically decreased neurite outgrowth on their appropriate substrates. To control for potential antibody-induced toxicity, RIgG (Fig. 7D), anti-fibronectin (Fig. 7E), or anti-laminin (Fig. 7F) was added to the media of DRG cultures plated on poly-lysine-coated coverslips. In all three cases, DRGs survived and were able to send out long processes. This suggests that the effect seen with the fibronectin and laminin antibodies on the fibronectin- and laminin-coated coverslips, respectively, is caused by a specific blockade of fibronectin- and laminin-mediated outgrowth and not by antibody-mediated toxicity.
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Figure 7. Polyclonal fibronectin (FN) and laminin (LN) antibodies are function blocking. A-C, G, Dissociated, adult DRGs were plated onto fibronectin-coated (A, B) or laminin-coated coverslips (C) and treated with control rabbit IgG (A), rabbit anti-fibronectin (B), or rabbit anti-laminin (C) for 3 d and then stained for
Fibronectin is critical for sensory axon regeneration in the corpus callosum
Because we had a function-blocking antibody against fibronectin, we used it in our slice culture model to determine how necessary fibronectin is for axonal outgrowth in white matter. The cultures were prepared as described above. Several hours after the dissociated DRGs were seeded onto the callosum, the fibronectin antibody was added both to the surface of the callosum and to the media. RIgG was added to alternate slices as a control. Although we were not able to visualize laminin in the corpus callosum via immunohistochemistry, we wanted to ensure that callosal laminin, which perhaps is present but difficult to visualize, is not playing a role in mediating outgrowth. Therefore, some slices were treated with either the function-blocking anti-laminin antibody or the appropriate concentration of RIgG as a control. Three days later, the slices were fixed and stained for GFP (for seeded neurons). GFP+ DRGs on RIgG-treated slices appeared similar to those on untreated slices (Fig. 8A) and displayed robust outgrowth on the callosum. The laminin antibody also did not have an effect on neurite outgrowth on the callosum (Fig. 8D,E). Furthermore, treating slices with antisense against thechain of laminin to inhibit laminin synthesis more broadly (Grimpe et al., 2002
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Figure 8. Anti-fibronectin, but not anti-laminin, decreases regeneration on white matter. After neuron seeding, the slice cultures were treated with control RIgG (A), anti-fibronectin (B), or anti-laminin (C) for 3 d. GFP immunohistochemistry is shown in A, B, and D. Blood vessels were also stained because the anti-rabbit secondary used to stain the GFP+ neurites also recognized the anti-laminin and anti-fibronectin antibodies. Although there was extensive outgrowth along the corpus callosum in RIgG- and anti-laminin-treated slices, there was a diminished amount of outgrowth in anti-fibronectin-treated slices. C, High-magnification image of a fibronectin-treated slice double stained for GFP (green) and GFAP (red). Although the GFAP+ astrocytes in the fibronectin-treated slices appeared smaller than in RIgG- or anti-laminin-treated slices (compare with Figs. 2 and 5 A, B), GFP+ neurites (green) were not able to grow well even on an intact, longitudinal astrocytic terrain (arrowhead). E, Outgrowth on white matter of slices treated with RIgG (n = 10), anti-fibronectin (n = 10), or anti-laminin (n = 5) was digitally traced. The pixel areas of the tracings were quantified and statistically compared using a Student's t test. Although there was no significant difference between RIgG- and anti-laminin-treated slices, anti-fibronectin statistically decreased outgrowth when compared with the RIgG-treated controls (*p < 0.0001). These findings suggest that astrocyte-associated fibronectin plays a critical role in regeneration on the corpus callosum. Scale bars: A, B, D, 100 µm; C, 25 µm.
Although it is possible that the abnormal astrocytic patterning may be partially responsible for the decreased outgrowth in anti-fibronectin-treated cultures, it seems unlikely that a change in substrate geometry is the sole reason for the reduced outgrowth. Many DRG neurites in slices treated with anti-fibronectin were short, despite the fact that they had a significant length of contiguous astrocytic terrain remaining ahead of them (Fig. 8C, arrowhead, green). Such curtailed outgrowth in the presence of antibody suggests that axons were directly affected by a lack of astrocytic fibronectin. Total neurite outgrowth per cell was decreased byTo further ensure that the astrocytic phenotype change seen in the anti-fibronectin-treated slice cultures is not caused by antibody-induced glial toxicity, RIgG, anti-fibronectin, or anti-laminin was added to confluent monolayers of astrocytes plated onto poly-lysine-coated coverslips. After 3 d, the cultures were fixed and stained for GFAP. With all three treatments, astrocytes looked healthy and remained confluent, suggesting that none of the treatments are toxic to astrocytes (data not shown). Unlike the antibody-mediated change in astroglial shape in the organotypic slice cultures, astroglial morphology in dissociated culture was comparable in all treatment groups.
Discussion
To investigate what cellular and molecular factors promote robust sensory axon regeneration in white matter, dissociated adult DRG and cortical neurons were seeded onto organotypic slices containing the corpus callosum. Although cortical neurons extended very short, branched MAP-2+ neurites, sensory neurons regenerated long axons within the degenerating myelin along host astrocytes. Fibronectin was associated with astrocytes both in P35 slices and in the adult callosum, despite literature suggesting that fibronectin is expressed only transiently during development (Stewart and Pearlman, 1987Using fresh organotypic slices satisfied our aim to develop an easily manipulated system to study axonal regeneration in white matter; however, the white matter within our in situ model may be different from that in vivo. Much of the slice is undergoing cell death, and although the astrocytes are clearly in a gliotic state, this type of reactive phenotype as well as the integrity of myelin in the slice may be quite different from that found in vivo, even after an axotomizing injury. Nonetheless, because the expression patterns of both fibronectin and laminin in cultured slices and perfused rat brain sections are similar, and DRGs in the in situ assay behaved similarly to neurons microtransplanted into white matter tracts in vivo, we speculate that the molecular and cellular environments encountered by the seeded neurons in our model are fundamentally similar to that in vivo.
Anti-fibronectin treatment of our slices caused a morphological change in callosal astrocytes not seen in those treated similarly on coverslips. It is likely that the mechanisms used by astrocytes to bind to the poly-lysine culture surface are different from those used to bind to each other in situ. In situ, astroglia may use fibronectin and its binding to astrocytic fibronectin receptors, including
5
The abundant expression of fibronectin in white matter may be a contributing factor to the failure of axon regeneration by central neurons in the adult. We observed that although adult sensory neurons displayed robust outgrowth, adult cortical neurons did not grow well on slices containing white matter. It has been shown that CNS neurons can grow well on laminin (Rogers et al., 1983
Interestingly, adult cortical neurons appeared to elaborate dendrites on the callosum. According to Goldberg et al. (2000a,b), the mature CNS neuron switches to a primarily dendrite-producing cell and focuses its transport mechanisms toward the production of dendrite-specific proteins. Also, Le Roux and Reh (1995
The finding that sensory neurons are able to regenerate at all in white matter appears to challenge a great number of studies in the literature which suggest that myelin-related proteins potently inhibit outgrowth and regeneration (Caroni et al., 1988
Footnotes
Received June 1, 2004; revised September 2, 2004; accepted September 3, 2004.This work was supported by National Institute of Neurological Disorders and Stroke Grants NS25713 (J.S.) and NS41383 (A.T.M.), the Daniel Heumann Fund, and the Brumagin Memorial Fund. We are grateful to Maryanne Pendergast for her excellent technical assistance with the confocal microscope and Dr. John Houlé for generously providing the computer software used for some of the quantification.
Correspondence should be addressed to Dr. Jerry Silver, Case Western Reserve University, Department of Neurosciences, 10900 Euclid Avenue, Cleveland, OH 44106. E-mail: jxs10{at}cwru.edu.
Copyright © 2004 Society for Neuroscience 0270-6474/04/249282-09$15.00/0
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