The Journal of Neuroscience, October 27, 2004, ():
Regulation of Brain Proteolytic Activity Is Necessary for the In Vivo Function of NMDA Receptors
J. Neurosci. Kvajo et al.
24: 9734
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Supplemental Figure.
Cell types expressing PN-1. (A) Distribution of PN-1 upregulating cells upon single whisker experience. PN-1 upregulating cells could be distinguished by their small, light blue nuclei. SMI32 antibody (brown), specific for layers III and V pyramidal neurons was used to distinguish cortical layers. Note that cells upregulating PN-1 are found mainly in the in layer II/III (arrowheads). (B) In layer III, PN-1 upregulating cells did not express SMI32 (empty arrowheads), whereas in layer V most of the SMI32 positive cells did (arrowheads) (C). (D) No inhibitory neurons in layers III or IV, detected with an anti-GAD antibody were X-gal positive (empty arrowheads). (E, F) Most of the GFAP-positive glial cells expressed PN-1 (arrowheads), but these were not the cells upregulating it upon sensory experience (empty arrowheads). (G) Some PN-1 upregulating cells co-expressed NG2, a chondroitin sulphate proteoglycan (arrowheads). Other cells were immunoreactive for NG2 and expressed PN-1 but appeared to be different from those upregulating it upon sensory experience (arrows). Scale bar, (A) 100 mm; (B-E), (shown in D) 50 mm; F), 10 mm, (G), 100 mm.
