The Journal of Neuroscience, November 10, 2004, ():
Axonal Regeneration and Lack of Astrocytic Gliosis in EphA4-Deficient Mice
J. Neurosci. Goldshmit et al.
24: 10064
Supplemental data
Files in this Data Supplement:
- supplemental material
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Fig. S1. Anterograde tracer injected into cervical spinal cord of unlesioned mice labels descending axon tracts on the ipsilateral but not the contralateral side.
Asterisks indicate injection site; arrows indicate the midline, the dotted line shows the edge of the contralateral side of the section; scale bar, 500µm
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Fig. S2. Anterogradely labeled spinal cords 6 weeks after lesion.
(a-d) Similar to Fig. 2a, panels show further examples of anterograde tracing 6 weeks after spinal cord lesion in another 4 EphA4-/- mice, with axons passing through and beyond the lesion site. In all panels rostral is to the right and caudal to the left. Dotted lines and arrows indicate the lesion site. Panel (ii) in all cases shows H&E stained adjacent sections, indicating the lesion site. Scale bars, 200µm.
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Fig. S3. Macrophage/microglial activation after spinal cord lesion.
The macrophage/microglial marker CD11b was used to examine activation of the cells at 1 week and 6 weeks after spinal cord lesion. There was no apparent difference in the activation of these cells in wildtype or EphA4-/- spinal cords.
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Fig. S4. EphrinB3 and EphA4 expression on spinal cord axons.
(a) As shown by immunostaining upstream of the lesion site at 6 weeks, ephrinB3 was expressed on multiple axons as well as on glial cells (arrowheads). The rabbit anti-ephrinB3 antibody was raised to a peptide corresponding to amino acids 281-299 of the C-terminus of human Ephrin-B3. (c-e) In unlesioned spinal cord EphA4 (c) was not expressed on GFAP-expressing astrocytes (b) but was expressed on motor neurons (c). Panel (e) is a merged image of panels (c & d). (b, f-h) EphA4 was expressed on lesioned axons upstream of the lesion site. Panel (f) shows anterogradely labeled axons (arrows) upstream of the lesion site in wildtype mice, which express EphA4 (g). Panel (h) is a merged image of panels (f & g). Scale bars in a, b & e, 50µm; in h, 125µm.
