The Journal of Neuroscience, November 17, 2004, ():
Nitric Oxide Synthase (NOS)-Interacting Protein Interacts with Neuronal NOS and Regulates Its Distribution and Activity
J. Neurosci. Dreyer et al.
24: 10454
Supplemental data
Files in this Data Supplement:
- supplemental material
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Supplementary fig. 1: Anti-NOSIP immunoreactivity in terminal dendrites of cultured neurons acutely treated with NMDA (1 µM) and glycine (10 µM) or tetrodotoxin (TTX, 0.5 µM). Phalloidin-TRITC labelling was used to counterstain the actin cytoskeleton. Scale bar represents 40 µm.
- supplemental material
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Supplementary fig. 2: Colabelling of brain sections of mice treated with 25 mg/kg kainate with anti-NOSIP and anti-GABA antibodies. (A) Staining of interneurons in the hippocampal molecular layer (arrow points to a co-labelled cell). (B and C) Staining of piriform cortex with anti-GABA antibody reveals co-labelling of interneurons (arrow) with anti-NOSIP antibody, but not of additional, NOSIP-positive cells abundant in layer I, which resembled stellate astrocytes in morphology (arrowheads). CA2 – CA2 sector of the pyramidal cell layer; I – cortical layer I. Scale bars represent 200 µm in A and B and 40 µm in C.
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Supplementary fig. 3: Changes in immunoreactivity for NOSIP (A) and nNOS (B) in cultured neurons at 40 h following treatment with kainate (20 µM). Phalloidin-TRITC was used to counterstain the actin cytoskeleton. Scale bars represent 20 µm in A and B.
