The Journal of Neuroscience, November 17, 2004, ():
A Genetic Switch for Epilepsy in Adult Mice
J. Neurosci. Krestel et al.
24: 10568
Supplemental data
Files in this Data Supplement:
- supplemental material -
Supplementary Figure 1. Regulated Cre expression and activity in mouse forebrain.
Cre expression and Cre-induced lacZ expression in Tga-CaMKII-tTA/LCR mice, which carried the Cre-indicator lacZ gene Rosa+/R26R to permit the visualization of Cre activity by X-Gal staining. At embryonic day 12.5 (E12.5) X-Gal staining was observed in telencephalon (te), mesencephalon (me) and spinal cord (sc) in whole embryos as well as hippocampus (hi) cortex (cx) and brain stem (pons and medulla) in paraffin sections of embryos. At postnatal days 0 (P0), P42, P20 and P52, the Cre expression pattern was dependent on the treatment of the mice with doxycycline (Dox). Cre expression was visualized by anti-Cre antibodies (Cre) and Cre activity by X-Gal staining (lacZ) in coronal brain sections containing piriform cortex (pi), amygdala (am), striatum (st) and the hippocampus (hi). Higher magnifications show the cellular Cre expression in the cortex and hippocampus (CA1, DG). Without Dox, Cre expression was abundant in the entire forebrain and substantially increased from P0 to P42. Cre expression could be suppressed by Dox treatment (P42 Dox). When Dox was present only during prenatal development (DoxP0), Cre expression by P20 was restricted to some cells in cortex, and to CA1 and DG regions in the hippocampus, but became more pronounced by P52, with Cre activity readily visible in CA1 and striatum, and to a lesser extent in the cortex and DG
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Supplementary Figure 2. Electrode positions after depth electrode EEG recording.
Cryostat brain sections from mouse number two (see Results) show the cell damage (arrows) in cortical layers 5/6 caused by the rostral cortical (C1) and the caudal cortical electrode (C2), positioned above the striatum and the hippocampus, respectively. The tip of the bipolar hippocampal electrode (H1/H2) was located in the dentate gyrus (arrow).
