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Figure 2. Transgenic TH overexpression: coexpression of TH and helper genes enhances the number of TH+ hNS1 cells. A, Microphotographs illustrating the increased number of TH+ cells after cotransfection of a TH-coding vector with other vectors coding for BDNF, GDNF, SOD1cit, Bcl-XL, or the empty vector, as a control. Scale bar, 100 µm. B, Quantification of the number of TH+ cells in the cotransfection experiments shown in A (n = 12; four independent samples in each of three separate experimental rounds); #p < 0.0001, Bcl-XL vs all other groups; *p < 0.005, different from the empty vector group (Student's t test).