The Journal of Neuroscience, December 8, 2004, ():
Behavioral Stress Modifies Hippocampal Synaptic Plasticity through Corticosterone-Induced Sustained Extracellular Signal-Regulated Kinase/Mitogen-Activated Protein Kinase Activation
J. Neurosci. Yang et al.
24: 11029
Supplemental data
Files in this Data Supplement:
- supplemental material
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Supplementary Figure S1. Temporal profiles of plasma ACTH (A) and corticosterone (B) levels before and after exposure to restrain-tailshock stress. Data are expressed as means ± SEM (n = 9).
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Supplementary Figure S2. U0124, K252b or BIS-V treatment does not affect the block of LTP and the facilitation of LTD by behavioral stress. A, Summary of experiments shows that in slices from stressed rats administrated with the inactive analogue U0124 (2 ?g/?l, bilateral intrahippocampal injection) 1 hr before the stress exposure, HFS failed to induce a reliable LTP that was not significantly different from that observed in slices from the stressed rats that received vehicle aCSF (10% DMSO) only. B, Summary of experiments shows that in slices from stressed rats that administrated U0124 before the stress exposure, LFS still induced a reliable LTD at the Schaffer collateral-CA1 synapses that was not significantly different from that observed in slices from the stressed rats that received vehicle aCSF (10% DMSO) only. C, Summary of experiments shows that in slices from stressed rats administrated with K252b (0.5 ?g/?l, bilateral intrahippocampal injection), a weaker inhibitor of Trk-type kinase, 1 hr before the stress exposure, HFS failed to induce a reliable LTP that was not significantly different from that observed in slices from the stressed rats that received vehicle aCSF (10% DMSO) only. D, Summary of experiments shows that in slices from stressed rats that administrated K252b before the stress exposure, LFS still induced a reliable LTD at the Schaffer collateral-CA1 synapses that was not significantly different from that observed in slices from the stressed rats that received vehicle aCSF (10% DMSO) only. E, Summary of experiments shows that in slices from stressed rats administrated with the inactive analogue BIS-V (0.2 ?g/?l, bilateral intrahippocampal injection) 1 hr before the stress exposure, HFS failed to induce a reliable LTP that was not significantly different from that observed in slices from the stressed rats that received vehicle aCSF (10% DMSO) only. F, Summary of experiments shows that in slices from stressed rats that administrated BIS-V before the stress exposure, LFS still induced a reliable LTD at the Schaffer collateral-CA1 synapses that was not significantly different from that observed in slices from the stressed rats that received vehicle aCSF (10% DMSO) only.
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Supplementary Figure S3. Representative immunoblot shows ERK1/2 MAPK phosphorylation after stress (S) with or without U0124, BIS-V and K252b treatment. The corresponding densitometric analysis is shown below V: vehicle CSF (10% DMSO).
