The Journal of Neuroscience, December 15, 2004, ():
Role of Actin Filaments in the Axonal Transport of Microtubules
J. Neurosci. Hasaka et al.
24: 11291
Supplemental data
Files in this Data Supplement:
- supplemental material
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Movie 1:? A pre-axonal cell body of a rat sympathetic neuron before and after filamentous actin depletion.? Before addition of latrunculin, microtubule plus ends do not typically invade the peripheral lamella of the cell.? Most of the GFP-EB3 excursions are outward or across the cell.? When the drug is added, the GFP-EB3 protein temporarily appears to dissociate from the plus-ends of the microtubules, but then recovers to again reveal growing microtubule plus ends.? As the filamentous actin is depleted, the microtubules can be seen to invade more peripheral regions of the cell and more of the plus ends extend outward toward the cell edge and explore the periphery.? The existing microtubules (lightly fluorescent) appear to re-configure into thicker bundles after actin depletion.? Frames were acquired every 4 seconds and playback rate is 24 frames per second.
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Movie 2:? A region of a neuronal cell body (rat sympathetic neuron) depleted of actin that was particularly favorable for imaging GFP-EB3 fluorescence.? At the periphery of the lamella, microtubule plus ends chaotically tumble about, similar to the tumbling observed in growth cones depleted of actin (see figure6K).? In the more interior regions of the cell, GFP-EB3 also labels the underlying microtubules lightly, revealing that growing microtubules use other microtubules as guides.? Frames were acquired every second and playback rate is 24 frames per second.
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Movie 3:? The distal segment and tip of an axon that extended in the absence of actin filaments.? The axonal shaft has a larger diameter; hence more GFP-EB3 comets are seen across the width of the axon.? No growth cone exists at the tip of the axon.? Instead, the tip of the axon is club-shaped and rich in microtubule plus-ends that extend to the end of the axon.? Most of the GFP-EB3 excursions are distally directed, indicating that microtubules can attain nearly correct polarity orientation in the absence of actin filaments (see figure 7).? Frames were acquired every second and playback rate is 24 frames per second.
